共查询到20条相似文献，搜索用时 62 毫秒
1.
杂交水稻种子纯度SSR指纹图谱标记鉴定技术研究 总被引：15，自引：1，他引：15
用SSR指纹图谱标记对10个杂交水稻组合的子一代种子纯度进行鉴定,并进行人为掺杂与田间种植验证.结果表明,分布于染色体1～12的232对微卫星引物中,有62对引物能够分别在1～10个杂交稻组合及其亲本中显示稳定的多态性,据此有效鉴定出杂交组合中的亲本种子;人为掺杂与田间种植验证均与室内SSR鉴定结果完全一一对应. 相似文献
2.
3.
4.
5.
6.
7.
SSR分子标记在杂交稻种子纯度精确鉴定中的应用 总被引：1，自引：0，他引：1
传统的田间小区种植纯度鉴定方法由于时间滞后不能满足种子企业的需求,而现有的SSR标记检测杂交水稻种子纯度的方法,虽能有效鉴定出杂交种的父、母本,但不能将一些混杂的杂株、串粉所致的杂交种区分开,也无法满足种子企业的全面质量信息需求。本文结合自身工作实践,介绍了一种利用筛选的多对SSR分子标记深入检测杂交水稻种子纯度及开展杂株类型定量鉴定的方法,这种方法不仅能更精确的检测杂交水稻种子纯度,还能将种子所含的杂株类型及其各自占比鉴定出来,为种子企业提供更准确、更全面的质量信息和可操作性强的加工处理办法。本方法广泛适用于各种子企业对主营骨干水稻品种的质量监控和生产经营决策。 相似文献
8.
玉米品种纯度SSR鉴定与田间鉴定的相关性 总被引：5，自引：0，他引：5
建立快速、准确鉴定玉米种子纯度技术是控制种子质量的有效措施。本研究针对SSR鉴定结果低于种植鉴定结果的问题,分析了可能的原因,提出了解决办法。利用筛选的特异标记测定了标记在原种、大田用自交系、套袋配制杂交种中的检出率,测定了2个杂交种(莱农14和莱农15)共16份样品的纯度。特异标记检出率随着自交系繁殖代数增加呈现逐渐降低的趋势;杂交种出苗率越低,SSR鉴定结果与田间鉴定结果的差值越大,是导致SSR检测纯度偏低的两个主要原因。将SSR结果与田间种植结果相比较,建立了SSR鉴定与田间种植鉴定的回归方程,可准确鉴定杂交种的纯度。 相似文献
9.
10.
11.
介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
12.
DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
13.
香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。 相似文献
14.
Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value. 相似文献
15.
16.
17.
南瓜降糖功能及其系列食品加工技术 总被引：3，自引：0，他引：3
阐述了南瓜在糖尿病辅助治疗方面的功能特性,介绍了南瓜降糖主要制品南瓜全粉、南瓜脆片、南瓜果酱和南瓜肉汁的工艺流程和操作要点。 相似文献
18.
母猪繁殖障碍成因分析 总被引：3，自引：0，他引：3
繁殖障碍是导致母猪淘汰的最主要因素,直接造成母猪非生产天数延长,繁殖猪群生产效率降低,严重影响猪场的经济效益。本文作者对引起母猪繁殖障碍的多方面因素,包括营养因素（如饲养水平、饲料品质、断奶日龄）、环境因素（如温度、湿度、通风、光照）、疫病因素、生产技术及管理因素进行了详细阐述。 相似文献
19.
Applicability of molecular markers in the context of protection of new varieties of cucumber 总被引：2，自引：0，他引：2
G. P. Bernet S. Bramardi D. Calvache E. A. Carbonell M. J. Asins 《Plant Breeding》2003,122(2):146-152
The growing number of candidate varieties presented every year at each national Plant Variety Protection Office and their decreasing genetic variability forces strategies to be adopted that will reduce costs without losing rigour when deciding about the acceptance or rejection of a candidate variety. Molecular markers have been envisaged as a reliable tool to establish differences, but can molecular markers be used for assessing distinctness? A comparison between a molecular and a morphological characterization of 41 seed samples belonging to 36 cucumber varieties and including several external controls has been carried out to investigate the applicability of molecular markers in the context of distinctness, uniformity and stability (DUS) tests and the protection of new varieties. Both types of character indicate the same relationships among main groups, Dutch and Beth‐Alpha types, and Gerking and Slice types, the correlation between distance matrices being only 0.6489. Varieties considered uniform for morphological traits proved not to be so for molecular markers. Therefore, molecular characterization is not offering the same estimates of uniformity and relatedness between varieties as does morphological characterization. External controls were used to establish distinction limits with morphological and molecular dendrograms to focus on those varieties, which were suspiciously similar. The results with cucumber show that molecular identity could be used to assess any lack of distinctness and so corroborate the morphological assessment of candidate varieties. The morphological trait ‘type’ provides distinct characterization of varieties and the molecular characterization of candidate varieties could be used to design better field experiments to assess distinction within each group of morphological cucumber type. 相似文献