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泥鳅几项正常血液指标及其与性别的关系 总被引：5，自引：0，他引：5
以60尾泥鳅为材料,测定了红细胞数、白细胞总数、血红蛋白值、白细胞分类记数、红细胞脆性与红细胞大小等正常血液指标,并统计了性别对其血液指标的影响。结果如下:红细胞数为(227 28±43 96)×104个 mm3;白细胞数为(2 56±0 86)×104个 mm3;血红蛋白含量为(12 26±1 89)g 100ml;白细胞分类计数中淋巴细胞占(28 67±8 27)%,血栓细胞占(26 46±5 33)%,中性粒细胞占(43 83±8 37)%;而单核细胞、嗜碱性白细胞以及嗜酸性白细胞观测到的数目很少;红细胞最大抵抗强度为(0 31±0 04)g 100ml;红细胞大小(长径×短径)为:(11 50±1 55)μm×(8 66±1 29)μm;红细胞核大小(长径×短径)为:(5 59±1 04)μm×(3 90±0 89)μm。性别对红细胞数量及红细胞核大小均有显著影响,对其它的血液常数指标则无显著影响。 相似文献
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乌鳢血液指标的研究 总被引：6，自引：2，他引：4
对乌鳢的红、白细胞数、血红蛋白等血液指标进行了研究。结果如下:乌鳢的红细胞数为(369.83±51.03)万个/mm3;白细胞数为(5.88±1.27)万个/mm3;血红蛋白为(9.54±0.61)g/100ml;白细胞分类计数中淋巴细胞占(58.23±5.67)%;嗜中性细胞占(28.10±4.36)%;单核细胞占(9.10±4.02)%;嗜碱性细胞和嗜酸性细胞观测到的数目很少;血沉为(1.50±0.12)mm/h;红细胞膜最大抵抗值为(0.38±0.02)%;红细胞大小为(10.02±0.52)μm×(7.01±0.24)μm(长径×短径);红细胞核为(4.22±0.27)μm×(1.96±0.18)μm(长径×短径)。 相似文献
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军曹鱼稚鱼外周血细胞及其形态学观察 总被引：4，自引：0，他引：4
以出膜后第60天的军曹鱼外周血液为材料,利用光镜显微技术研究了其血细胞的种类和形态。外周血液包括5种类型的血细胞,红细胞、淋巴细胞、单核细胞、嗜中性粒细胞和血栓细胞。血细胞计数测得红细胞密度为(2.97±0.82)×109ind.mL-1,白细胞密度为(1.39±0.94)×106ind.mL-1,淋巴细胞、嗜中性粒细胞、单核细胞和血栓细胞在白细胞中所占的比例分别为(53.00±8.51)%、(17.59±4.28)%、(4.78±1.37)%和(24.63±4.08)%。未见到嗜酸性粒细胞和嗜碱性粒细胞。血细胞计数结果表明,红细胞数量远远大于白细胞,白细胞中淋巴细胞数量最多,单核细胞数量最少。实验还观察到处于分裂状态的红细胞、细胞表面有伪足样胞突的小淋巴细胞和细胞表面有许多微绒毛突起的大淋巴细胞、核呈多种形态的单核细胞和中性粒细胞及不同形态的血栓细胞等。 相似文献
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《水产科学》2015,(4)
对20尾点带石斑鱼进行了血液指标、血细胞分类和发生的研究。血液指标经测定:红细胞密度为(1.89±0.48)×106个/mm3,白细胞密度为(1.65±0.34)×104个/mm3;血红蛋白含量为(7.11±0.67)g/L;红细胞的渗透脆性为(0.54±0.02)%,红细胞比容为(36.7±0.67)%,红细胞沉降率为(2.50±0.25)mm/h。利用Wright’s-Giemsa混染法对点带石斑鱼外周血液以及肝脏、脾脏、头肾和体肾四种造血组织染色观察发现,在外周血涂片上可区分出红细胞(包括未成熟的红细胞)、血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞,未发现嗜酸性粒细胞和嗜碱性粒细胞。其中大淋巴细胞、小淋巴细胞、嗜中性粒细胞和单核细胞在白细胞中所占的比例分别为(23.13±6.99)%、(48.97±7.42)%、(21.03±4.42)%和(6.87±2.27)%。点带石斑鱼的头肾、体肾和脾脏可以产生各种类型的血细胞,是主要的造血器官;肝脏未观察到原红细胞。 相似文献
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对40尾野生青石爬鮡进行了血液生化指标、血细胞分类与发生研究。测得血浆的总蛋白为(48.35±3.60) g/L,白蛋白为(8.73±0.54) g/L,球蛋白为(39.63±3.10) g/L,血浆白蛋白与球蛋白比为(0.22±0.01),甘油三酯为(5.05±2.14) mmol/L,总胆固醇为(16.66±3.64) mmol/L,极低密度脂蛋白为(1.01±0.43) mmol/L,葡萄糖为(5.27±2.43) mmol/L。血液生化指标检测结果表明,青石爬鮡血浆中总蛋白与球蛋白、甘油三酯与胆固醇较高,葡萄糖较低,具有活动量较大和冷水性鱼类的特点,主要利用脂肪和蛋白质作为能量。细胞学显示出其外周血液包含红细胞,核影,淋巴细胞,血栓细胞,嗜中性粒细胞和单核细胞。血涂片计数为红细胞96.58％,其中成熟红细胞占65.92％,幼稚红细胞占30.66％。白细胞为3.42％,其中嗜中性粒细胞0.14％、单核细胞0.07％、淋巴细胞2.37％、血栓细胞0.85％。白细胞中淋巴细胞和血栓细胞数量较多。原始血细胞主要在中肾和头肾中,在脾脏中也有部分原始白细胞,而肝脏和外周血液中未见原始血细胞。中肾和头肾是青石爬鮡的主要造血器官,其中红细胞主要由头肾产生,白细胞主要由中肾产生。脾脏亦产生少量白细胞。 相似文献
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采用血细胞常规染色和细胞化学染色对厚颌鲂(Megalobrama pellegrini)外周血细胞形态及细胞化学特征进行观察。结果显示:厚颌鲂外周血细胞可分为红细胞、血栓细胞、单核细胞、淋巴细胞和三种类型粒细胞。红细胞有幼稚、成熟和衰老三种形态,其中幼稚和衰老红细胞较少见;血栓细胞数量最多,其次是淋巴细胞。粒细胞中Ⅰ型和Ⅱ型较常见,Ⅲ型粒细胞少有。细胞化学染色显示幼红细胞、血栓细胞和淋巴细胞均呈ACP弱阳性;单核细胞呈ACP和ANAE阳性;Ⅰ型粒细胞是嗜中性粒细胞,POX、ACP和ANAE阳性;Ⅱ型粒细胞是PAS-GL,PAS强阳性和ACP阳性;Ⅲ型粒细胞是Ⅰ型粒细胞的晚期形态。厚颌鲂的PAS-GL可能是嗜酸性/嗜碱性粒细胞,而不是嗜碱性粒细胞的前体。 相似文献
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淡水鲳六项血液指标的测定及血细胞结构的显微观察 总被引：13，自引：0，他引：13
本文对淡水鲳六项血液指标进行了测定,结果是红细胞(RBC):179×10~4/mm~3;白细胞(WBC):7.22×10~4/mm3,血红蛋白含量(Hb):6.4g/100ml;红细胞脆性(开始溶×完全溶):0.333Nacl％×0.217Nacl％;红细胞大小(长×短):13.69×10.20um~2;红细胞核大小(长×短):5.51×3.39um~2。白细胞的百分式中以淋巴细胞为主,嗜中性细胞次之,嗜碱性、嗜酸性粒细胞和单核细胞数量极少。此外,还对淡水鲳的血液指标与其它养殖鱼类的血液指标进行了比较,并对其数量、形态、生理功能之间的关系进行了探讨。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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