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1.
NDVLaSota、V4疫苗免疫鸡及免疫攻毒鸡的主要特异性血清抗体动态变化规律比较 总被引：2，自引：0，他引：2
本研究应用间接ELISA方法对新城疫LaSota和V4疫苗免疫SPF鸡及免疫后攻毒SPF鸡的血清中新城疫病毒（NDV）特异性HI抗体、IgM和IgG抗体水平的动态变化进行了检测。结果表明，V4较LaSota疫苗免疫鸡HI抗体提前3天左右出现，但除高峰期（1周左右）外，其HI抗体水平均低于LaSota免疫鸡。LaSota免疫鸡。LaSota疫羁免疫鸡血清中NDV特异性IgM和IgGr抗体高峰较V4免疫鸡提前约2周出现，攻毒后，LaSota免疫鸡血清中特异性IgG和IgG回忆应答显著，而V4免疫鸡IgM和IgG回忆应答不明显。 相似文献
2.
新城疫La Sota、V4疫苗免疫鸡和免疫攻毒鸡的特异性IgA抗体动态变化规律比较 总被引：4，自引：0，他引：4
应用间接ELISA对新城疫LaSota、V4疫苗免疫鸡及免疫攻毒鸡的IgA抗体动态变化进行了测定和比较。试验表明，LaSota和V4免疫鸡泪液中特异性IgA均在免疫后第5天出现，8天开始明显升高，与血清中IgG出现时间相似，免疫后21天达到高峰。LaSota免疫鸡的泪液IgA抗体水平略高于V4免疫鸡；而两免疫组哈德氏腺(HG)中的特异性IgA高峰出现较迟。免疫攻毒鸡泪液中的特异性IgA抗体水平均先呈短暂的升高，之后下降的趋势，而HG中的IgA则首先表现降低，之后很快升高，5天后趋于下降，两攻毒组差异不显著，对IgA回忆应答均不明显。 相似文献
3.
ELISA检测鸡新城疫病毒特异性IgM抗体的研究 总被引：3，自引：0，他引：3
以新城疫病毒单克隆抗体包被板,用10％小牛血清-PBS封闭后,捕获尿囊液中的新城疫病毒作固相抗原.在此板上应用酶标抗鸡IgM单克隆抗体进行间接ELISA试验检侧鸡血清中新城疫病毒的特异性IgM抗体.试验证明该方法特异性强、敏惑性高.兔抗新城疫病毒阳性血清可特异性阻断反应,将新城疫病IgM阳性血清用2-ME处理可使ELISA反应呈阴性,与禽源多杀性巴氏杆菌鸡IgM阳性血清、鸡传染性支气管炎病毒IgM阳性血清无交叉反应.该试验可检测到La Sota免疫后3天鸡血清中的特异性IgM,对鸡新城疫病毒IgM阳性血清的检测效价可达1:320以上,并可检测到临床新城疫病鸡血清中的特异性IgM抗体. 相似文献
4.
植物血凝素对鸡新城疫疫苗免疫效果的影响 总被引：4，自引：0，他引：4
试验对植物血凝素在艾维菌雏鸡新城疫疫苗免疫过程中的效果进行了研究.经过雏鸡新城疫母源抗体水平的检测后.选择抗体滴度约为2.5 log2的雏鸡90羽,随机分成3组,分别为溶剂对照组、单免组和PHA组.单免组和PHA组分别于7.21 d接种Ⅳ系LaSota弱毒疫苗,PHA组还同时肌肉注射植物血凝素0.01 mL/只.各组鸡于第1次免疫后的14.21,28.35,42.49和56d测定血液中鸡新城疫特异性抗体水平.结果显示,经2次免疫后PHA组鸡的抗体滴度比单免组和对照组鸡的高.差异显著(P<0.05),说明PHA能显著增强雏鸡对LaSota弱毒苗的免疫反应. 相似文献
5.
为研究左旋咪唑对鸡新城疫LaSota疫苗免疫效果的影响,试验采用1日龄健康AA+肉仔鸡200只,随机分为4组,每组50只,第4天开始,Ⅰ组、Ⅱ组和Ⅲ组分别以左旋咪唑5、10和20mg/kg·W,一天1次,连饮3d,Ⅳ组为对照组。7日龄时I组、Ⅱ组、Ⅲ组和Ⅳ组分别按说明书注射新城疫LaSota疫苗。分别于14、21、35、42日龄采用反向间接血凝抑制试验、ANAE+法测定新城疫抗体水平和免疫器官中T淋巴细胞数,同时测定免疫器官指数。结果表明,左旋咪唑中、低剂量组能明显提高新城疫LaSota疫苗特异性免疫应答抗体水平,免疫器官指数、血液中ANAE+T淋巴细胞百分率显著提高。说明中、低剂量左旋咪唑对鸡新城疫LaSota疫苗有免疫增强作用,且适合剂量为10mg/kg·W。 相似文献
6.
新城疫是危害我国养禽业的重要疫病之一,新城疫病毒(NDV)流行毒株有多种基因型,其中基因Ⅶ型毒株流行较为普遍。本研究利用流行病学调查中筛选出的NDV LY1分离株(基因Ⅶ型)和传统的LaSota株(基因Ⅱ型)与油佐剂按1∶3比例混合乳化,分别制成油包水型LY1、LY1+LaSota和LaSota油乳剂灭活苗。免疫试验结果显示,LY1灭活疫苗组HI抗体效价较低,LY1+LaSota疫苗组HI抗体水平稍高于商品化LaSota疫苗组(P>0.05),抗体产生规律相似;利用LY1分离株攻击,LY1+LaSota株组免疫保护率达100%,明显高于LY1分离株灭活疫苗组及商品化灭活疫苗组。本试验为新城疫新的疫苗研究开发奠定了基础。 相似文献
7.
通过抽取鸡血清作血凝(HA)实验来进行新城疫检疫,作血凝抑制(HI)实验进行抗体水平的监测,可以了解养鸡场的抗体水平和掌握其动态变化。本次检测是从门源县某鸡场随机采取的60个样品血清进行抗体水平监测,结果样品抗体水平大于5log2的占67.65%为免疫合格部分。而整场免疫不合格,注射疫苗后为能达到最佳效果,需要补免和再次免疫,以防新城疫病毒入侵鸡场。 相似文献
8.
CpG ODN对鸡新城疫LaSota活疫苗的免疫增强效应 总被引：4，自引：0，他引：4
将3种不同的未甲基化CpGODN分别与新城疫LaSota活疫苗混合后，经滴鼻和点眼免疫鸡，通过检测鸡血清中HI抗体、外周血T淋巴细胞增殖活性、诱导巨噬细胞分泌NO含量，以及刺激外周血淋巴细胞表达IFN-γ、IL-6与IL-1β mRNA量，分析各CpGODN对新城疫LaSota活疫苗免疫效果的影响。结果表明，经2次免疫后，含GTCGTT核心基序的CpG ODN1组，鸡血清平均HI抗体效价最高达8．2log2、淋巴细胞刺激指数达9．836、NO分泌量达35．833μmol／L，分别比疫苗单独免疫组高出2个滴度（P〈0．05）、4．4（P〈0．01）、27．6μmol／L（P〈0．01）；含GACGTT核心基序的CpG ODN2组增强作用不明显．与疫苗单独免疫组无差异；而CpGODN3的免疫刺激活性由于受其侧翼序列的影响，作用明显减弱甚至丧失。对细胞因子的影响，CpGODN3组IFN-γ mRNA表达量稍高于CpG ODN1组，而其余细胞因子均以CpG ODN1组表达水平最高（P〈0．05）。由此证明CpG ODN1能显著增强鸡对新城疫LaSota活疫苗的体液和细胞免疫反应，可以作为高效的免疫增强剂。 相似文献
9.
低毒力新城疫抗原抗体复合物疫苗研究 总被引：2，自引：1，他引：1
用低毒力新城疫病毒LaSota株作为复合物疫苗抗原,与新城疫特异性中和抗体混匀配制了不同比例的8种复合物疫苗,用1日龄SPF雏鸡进行免疫效果试验。试验l组～8组分别免疫复合物疫苗1～8,试验9组免疫常规新城疫活疫苗,试验10组为空白对照组。免疫后3周采血测定HI抗体效价,同时用新城疫强毒北京株F48E9攻击。试验l组～10组HI抗体效价分别为2^3.6、2^3.1、2^2.3、2^4.85、2^4.85、2^5.0、2^5.0、2^4.5、2^5.1、2^2.14。攻毒后1组～10组鸡的死亡率分别为30％、50％、90％、10％、0％、0％、0％、0％、09,6、100％。结果表明,低毒力新城疫病毒LaSota株与特异性抗体制备的复合物疫苗,可以减轻疫苗的毒副作用,提高疫苗的安全性,其免疫效果比常规活苗好。 相似文献
10.
利用小鼠骨髓瘤Sp2/0细胞系与纯化鸡IgG免疫的BALB/C小鼠脾细胞融合,成功地建立了5株能持续分泌抗鸡IgG单克隆抗体(McAbs)的杂交瘤细胞株。选择产生抗体效价高、生长快的C_(44)株,扩大培养后注射于小鼠制备腹水,经硫酸铵盐析粗提后,用HRP标记,制备出McAbs酶结合物。将制备的McAbs酶结合物和多克隆抗体(PcAbs)酶结合物,采用间接ELISA,对35份鸡血清中鸡新城疫病毒(NDV)抗体以及19份鸡血清中鸡嗜血杆菌(HG)抗体进行检测,McAbs和PcAbs两者的相关系数分别为0.928和0.921,证明制备的抗鸡IgG McAbs具有特异性强、效价高的特点. 相似文献
11.
ZHANG Zhen-hua LI Lin ZHANG Jian-wei SHEN Jia SHI Ai-hua HUANG Feng-jun ZHENG Xiao-lan JIANG Bei-yu 《中国畜牧兽医》2016,43(5):1374-1378
1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens. 相似文献
12.
Wambura PN 《Tropical animal health and production》2009,41(2):149-152
The efficacy of green-coloured (GC) I-2 Newcastle disease vaccine was determined in the present study. I-2 vaccine was mixed
with a green coloured dye and stored at 4°C for 6 months while assayed for the virus infectivity at a monthly interval. Chickens
were vaccinated with the GC vaccine by eye drop. Serum samples were collected from all birds before and after vaccination
at weekly interval for 4 weeks and tested for haemagglutination-inhibition (HI) antibody against Newcastle disease virus (NDV). These chickens were challenged with NDV virulent strain four weeks after vaccination. The results showed that there
was no difference between the infectivity titres of GC and uncoloured vaccines. However, chickens vaccinated with GC vaccine
produced higher HI antibody titres than chickens vaccinated with uncoloured vaccine. Results from the challenge trial showed
that all vaccinated chickens survived whereas all unvaccinated chickens died. The findings from this study have shown that
the GC vaccine is safe and produced protective antibodies against NDV in vaccinated chickens.
Wambura, P. N., 2008. Protective antibody response produced by the chickens vaccinated with green coloured thermostable Newcastle
disease virus. Tropical Animal Health and Production. 相似文献
13.
Naoyuki AIHARA Noriyuki HORIUCHI Nanase HIKICHI Mariko OCHIAI Yuko HOSODA Yoko ISHIKAWA Yoko SHIMAZAKI Koji OISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):913-918
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV. 相似文献
14.
鸽禽Ⅰ型副粘病毒油佐剂灭活苗对雏鸡免疫效果评价 总被引：1，自引：1，他引：0
用鸽Ａ／ＰＭＶ－１油佐剂灭活苗与ＮＤＶ油佐剂灭活苗分别免疫雏鸡,免疫后２１ｄ抗体水平达到峰值,免疫后４２ｄ用新城疫强毒对两种疫苗免疫鸡分别进行攻击,鸽Ａ／ＰＭＶ－１油佐剂灭活苗免疫组保护率为７３．３３％,ＮＤＶ油佐剂灭活苗免疫组保护率为９９．６７％。 相似文献
15.
IG BELL PJ NICHOLLS C. NORMAN AINI IDERIS† GM CROSS† 《Australian veterinary journal》1991,68(3):97-101
Meat chickens housed on a commercial broiler farm in Australia were vaccinated once at 10 to 11 days-of-age by aerosol with live V4 Newcastle disease virus (NDV) vaccine. Groups of vaccinated and unvaccinated birds were flown to Malaysia, where they were challenged with a virulent strain of NDV. Survival rates in vaccinated chickens challenged 7, 14, 21 or 31 d after vaccination were 0.47, 0.77, 0.97 and 0.92, respectively. All unvaccinated chickens died due to Newcastle disease (ND) following challenge. Chickens in Australia and Malaysia were bled and the serums tested for haemagglutination-inhibiting (HI) antibody to NDV. Many vaccinated birds with no detectable antibody, and all birds with a log2 titre of 2 or greater, survived challenge. The results showed that this V4 vaccine induced protective immunity in a significant proportion of chickens within 7 d of mass aerosol vaccination. This early immunity occurred in the absence of detectable circulating HI antibody. Non-HI antibody mediated immunity continued to provide protection up to 31 d after vaccination. Almost all vaccinated birds were protected within 3 w of vaccination. It is concluded that the V4 vaccine is efficacious and could be useful during an outbreak of virulent ND in Australia. 相似文献
16.
本研究以新城疫病毒(NDV)V蛋白羧基端结构域(Vc)的重组蛋白为包被抗原,建立了用于检测NDV V蛋白抗体的间接ELISA方法,并采用该方法检测了鸡群免疫或接毒后血清中的V蛋白抗体水平。结果显示:两组不同NDV灭活疫苗组在免疫后的3周内检测结果均为阴性;两组灭活疫苗免疫3周后再人工感染NDV强毒的鸡群,攻毒后第7、14和21 d,NDV阳性率分别为60%、80%、70%和50%、80%、70%;两组不同的NDV弱毒疫苗免疫组鸡群,仅在免疫后第21 d阳性率分别为20%和10%。以上结果表明,NDV疫苗免疫组与强毒感染组的V蛋白抗体阳性率存在明显差异,本方法可在群体水平上区分新城疫疫苗免疫与强毒感染鸡群,为NDV血清学诊断和流行病学调查提供了一种新的检测手段。 相似文献
17.
18.
鸡新城疫重组鸡痘病毒活疫苗的免疫持续期和加强免疫研究 总被引：1，自引：0，他引：1
本研究旨在评价表达新城疫病毒（NDV）血凝素-神经氨酸酶（HN）基因的重组鸡痘病毒（rFPV-12LSHN）活疫苗的免疫持续期和加强免疫对疫苗免疫效力的影响。用rFPV-12LSHN活疫苗免疫14日龄SPF鸡,103PFU/羽,7d即可检测到NDV HI抗体应答,对NDV强毒F48E8株攻毒保护率达100%。一次免疫18周后,对NDV强毒攻击依然提供完全保护。鸡痘病毒（FPV）疫苗免疫4周,再接种rFPV-12LSHN活疫苗,攻毒保护率降低至50%。相反,rFPV-12LSHN免疫4周,随后二次免疫可显著提高对NDV的体液免疫应答水平（P〈0.01）,对NDV强毒攻击的保护率仍然为100%。结果表明,表达NDV HN基因的重组鸡痘病毒（rFPV-12LSHN）活疫苗,能够快速建立坚强免疫力,免疫持续期至少可达18周,rFPV-12LSHN的二次免疫可以提高疫苗的免疫力。 相似文献
19.
为提高新城疫病毒（Newcasti Disease Virus,NDV）F基因DNA疫苗的表达量,增强其免疫效果。按照鸡体内偏嗜性密码子人工合成了NDV F48E9株的F基因optiF,插入到真核表达载体pVAX1中获得pVAX1-optiF。将F48E9株的F基因pVAX1-F与pVAX1-optiF分别转染COS-7细胞,72h后间接免疫荧光和Western blot检测细胞中瞬时表达的F蛋白。将质粒pVAX1、pVAX1-F和pVAX1-optiF以200μg／只的剂量分别股四头肌多点注射18只2周龄SPF鸡,2周后加强免疫1次,同时设立PBS对照。二免2周后每组取12只鸡以100EID50的F48E9株NDV强毒进行攻毒,评价这2种DNA疫苗的免疫效果。结果表明,F基因密码子的优化可显著提高NDVDNA疫苗诱导的保护性体液免疫和细胞免疫应答水平,攻毒后所有pVAX1-optiF免疫鸡均获得保护（12／12）,而pVAX1-F组保护率只有17％（2／12）。DNA疫苗的免疫效果与抗原基因的表达量及表达抗原的免疫原性有直接关系。与未经修饰的F基因相比,修饰后的F基因体外瞬时表达水平明显提高。 相似文献
20.
从山东省发病鸡群分离鉴定了一株新城疫病毒(NDV),命名为SDLY01。经蚀斑纯化后进行毒力测定和序列分析表明分离株SDLY01属于基因Ⅶ型NDV强毒。20只7日龄SPF鸡免疫新城疫活疫苗LaSot a后14 d分别用NDV标准强毒F48E8和分离株SDLY01攻毒,同时设同日龄SPF鸡为对照组,未免疫任何疫苗。攻毒后观察10 d,免疫组在攻毒后食欲、精神均正常;对照组在攻毒后2～4d发病死亡,并表现ND典型的临床症状和病理变化。攻毒后第3、5、7、9 d对免疫组试验鸡取喉头、泄殖腔棉拭进行病毒分离,F48E8攻毒组病毒分离均为NDV阴性,SDLYO1攻毒组第5 d病毒分离NDV阳性,第3、7和9d病毒分离阴性。本研究结果表明LaSot a活疫苗对F48E8和SDLY01均能提供100%免疫保护,但不能完全抑制基因Ⅶ NDV分离株在体内的复制和排毒。 相似文献