首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 312 毫秒
1.
国内外很多学者都在进行着传染性法氏囊(Infectious bursal disease,IBD)各种基因工程疫苗的研制,IBDV-vp2基因在多种载体中得到表达并取得了较好的保护效力。本实验室已构建了表达IBDV-vp2基因的重组马立克氏病病毒。本研究对该重组马立克氏病病毒疫苗的遗传稳定性以及对SPF鸡和含有母源抗体的商品鸡的免疫保护作用进行了评价。结果表明,103 PFU和104 PFU剂量的重组病毒免疫后对SPF鸡抗传染性法氏囊标准强毒的保护率分别为53%和73%。104 PFU剂量的重组病毒免疫后对含有母源抗体的商品鸡抗传染性法氏囊标准强毒的保护率为87%,结果显示重组疫苗具有一定的应用前景。  相似文献   

2.
将鸡传染性法代囊病病毒(IBDV)BX株抗原和IBDV高免血清按一定比例混合,初步制备了无菌的鸡传染性法氏囊病免疫复合物疫苗1~5(分别含有32、8、4、0.5及0.125单位IBDV中和抗体),用鸡传染性法氏囊病免疫复合物疫苗1~5及BX株活疫苗分别免疫1日龄低母源抗体水平雏鸡,免疫后9 d观察法氏囊病变,免疫后28 d采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后9 d,复合物疫苗1~3免疫组鸡法氏囊正常,复合物疫苗4、5免疫组和BX株活疫苗免疫组分别有2/10、4/10和5/10的试验鸡法氏囊出现了病变;免疫后28 d,复合物疫苗1~5免疫组和BX株活疫苗免疫组IBDV中和抗体效价分别为8.34log2、9.60log2、9.21log2、7.88log2、9.50log2和9.12log2,攻毒保护率分别为90%、100%、100%、80%、90%和80%。试验结果表明鸡传染性法氏囊病免疫复合物疫苗2、3(分别含8、4单位IBDV中和抗体)免疫1日龄低母源抗体水平的雏鸡效果最好,攻毒保护率能达到100%。  相似文献   

3.
将鸡传染性法氏囊病免疫复合物疫苗和BX株活疫苗分别免疫1日龄中等母源抗体水平京红雏鸡,同时免疫1日龄SPF雏鸡作对照,免疫后第9天观察法氏囊病变,免疫后第28天采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后第9天,免疫复合物疫苗组SPF雏鸡和京红雏鸡法氏囊均正常,BX株活疫苗组SPF雏鸡法氏囊出现了萎缩病变;免疫后第28天,对照SPF雏鸡和京红雏鸡的免疫复合物疫苗组、BX活疫苗组IBDV中和抗体效价分别为9.2log2、8.5log2和 7.2log2、4.4log2,攻毒保护率均为100%。试验结果表明免疫复合物疫苗免疫1日龄中等水平母源抗体雏鸡效果很好,能产生较高的中和抗体,攻毒保护率能达到100%。  相似文献   

4.
Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%.  相似文献   

5.
One-day-old moderate maternal antibody Jinghong chickens and SPF chickens were vaccinated with infectious bursal disease (IBD) immune complex (IC) vaccine and BX strain live vaccine,respectively,pathological changes of bursa of fabricius in chickens were observed at the 9th day after immunization.On the 28th day after immunization,blood samples were taken and the IBDV neutralizing antibody were tested,meanwhile experimental chickens were challenged with high virulent IBDV,the protective rates of experimental groups were calculated.The results showed that at the 9th day after immunization,the bursa of fabricius in SPF and Jinghong chickens were normal in IC vaccine group,however atrophic changes of bursa of fabricius in BX strain live vaccine group were shown.At the 28th day after immunization,the IBDV neutralizing antibody of IC vaccine group and BX strain live vaccine group in SPF and Jinghong chickens were 9.2log2,8.5log2,7.2log2 and 4.4log2,respectively,while 100% protection rates were provided.The results provided that IC vaccine had better immunity effect on one-day-old moderate maternal antibody chicken,high levels of neutralizing antibodies against IBDV were produced and protection rates were 100%.  相似文献   

6.
以脂质体转染技术构建了表达鸡传染性法氏囊病病毒（ＩＢＤＶ）ＶＰ２基因的重组鸡痘病毒ＦＰＶ－ＶＰ２，该病毒在鸡胚成纤维细胞及鸡体内均能稳定产生子代病毒，经翅皮下５×１０５ＰＦＵ／羽免疫１日龄ＳＰＦ鸡，免疫后４周以１００ＬＤ５０／羽ＩＢＤＶ超强毒株Ｇ株攻毒，获得了５／６的保护，但不能有效预防临床发病及法氏囊受损萎缩。实验结果证明了ＶＰ２是ＩＢＤＶ的宿主保护性抗原，提示Ｔ细胞介导的免疫可能在ＩＢＤＶ的免疫中起着较为重要的作用。本研究为ＩＢＤＶ重组病毒疫苗研制进行了有益探索。  相似文献   

7.
对利用IBDV地方野毒株所制备的囊毒和胚毒二价灭活油乳苗免疫效果进行了比较研究。结果表明,所制备的囊毒灭活苗免疫效果显著优于胚毒灭活苗和活疫苗,在接种后各个时期所产生的抗体效价均显著高于其它两种疫苗(P<0.01),最高AGP效价可达4.8log2;胚苗的免疫效果虽然差于囊苗,但明显优于活疫苗,当活苗免疫组鸡血清中已无可测性抗体时,胚苗组仍具有1.0log2的AGP效价。在攻毒试验中,囊苗组鸡对IBDV标准毒、两株野毒株的攻击均100%地获得了保护;胚苗组也均可获得80%的保护,这两种由地方毒株所制备的疫苗均未呈现出对不同毒株抵抗性的不同,而活苗组对不同毒株的攻击则分别只有53.3%、40%和46.7%的保护率,呈现出一定的差异性。  相似文献   

8.
鸡传染性法氏囊病(IBD)是由鸡传染性法氏囊病病毒(IBDV)引起的雏鸡的一种高度接触性传染病,自1962年报道以来,世界上主要养禽国家和地区均有流行,给养禽业带来严重经济损失。目前,IBD防控的主要方法是采用疫苗接种,使易感雏鸡获得主动或被动免疫保护,因此疫苗的质量对临床上IBD的防控起着至关重要的作用。虽然各国均有较好的商品化疫苗,但随着IBDV毒株的不断变异,商品化疫苗的抗原性与流行毒株不能完全匹配,临床上免疫失败时有发生,因此迫切需要研发与临床流行毒株相匹配的新型疫苗用于IBD的防控。对近期IBD的基因缺失苗、亚单位疫苗、DNA疫苗以及活载体疫苗等新型疫苗的研究进展进行概述,以此为IBD新型疫苗的研究提供参考。  相似文献   

9.
CpG寡核苷酸对IBDV VP2基因真核表达质粒免疫增效作用   总被引：1，自引：1，他引：1  
以传染性法氏囊病病毒(IBDV)VP2蛋白基因表达质粒DNA为免疫原，以CpG的寡核苷酸(CpG-0DN)为免疫佐剂，肌肉注射于14日龄SPF鸡，1周后加强免疫1次，2次免疫后15d和21d分别测定血清ELISA抗体效价，并于免疫后21d用IBDV99儿强毒株攻毒和进行病理学观察。结果显示，(1)VP2基因重组质粒DNA与CpG共同免疫组的ELISA抗体水平明显高于VP2重组质粒免疫组；(2)IBD弱毒苗与VP2重组质粒免疫组抗体水平明显高于VP2重组质粒免疫组，且比VP2基因重组质粒DNA与CpG共同免疫组略高；(3)VP2基因重组质粒DNA与CpG共同免疫组及IBD弱毒苗与VP2重组质粒免疫组可明显降低IBDV强毒攻击后引起的急性发病率和死亡率。由此表明，CpG寡核苷酸对IBDV VP2蛋白基因真核表达质粒免疫具有明显增强作用，有很大的应用前景。  相似文献   

10.
The effect of the infectious bursal disease (IBD) live virus vaccine on the immune response of chicken was evaluated by the assessment of antibody response following vaccination as well as resistance to challenge with virulent virus. Birds were vaccinated at various ages and later challenged with a heterologous vaccine (NDV) or wild-type IBD virus. The BF was examined for histological changes at regular intervals. Antibody levels to NDV were monitored.

Significantly higher mortality rates were observed in birds vaccinated with IBD vaccine than unvaccinated birds (P < 0.01) following challenge, BF from vaccinated birds showed marked lymphocyte depletion and cellular infiltration with mononuclear cells.

Intraocular NDV (NDV-i/o) vaccine given at day old largely prevented the immunodepressive effect of IBD vaccination on NDV vaccine. Groups that received IBD vaccine on day 14 but no NDV i/o suffered higher mortality (41.2%) and showed lower antibody response than those vaccinated on day 1 (0%) or controls which did not receive IBDV (11.8%).  相似文献   


11.
鸡传染性法氏囊病（IBD）是一种严重危害养禽业的高度致死性和免疫抑制性传染病。为研制IBD重组火鸡疱疹病毒（HVT）活载体疫苗,本研究构建了表达鸡传染性法氏囊病病毒（IBDV）保护性抗原VP2基因的重组HVT并对其体外生物学特性进行了分析。通过RT-PCR扩增IBDV超强毒株VP2基因并克隆入pCI载体,获得重组真核表达质粒pCI-VP2。用限制性内切酶将携带CMV启动子的VP2基因表达框架切下,连接于入门质粒pENTR,构建获得重组入门质粒pENTR-VP2。将pENTR-VP2与HVT重组黏粒H3-Kan/ccdB进行LR重组反应,构建重组表达黏粒H3-VP2。用H3-VP2与其他4个相互重叠并覆盖HVT全基因组的黏粒共同转染鸡胚成纤维细胞（CEF）,拯救获得重组病毒rHVT-VP2。将重组病毒在CEF中连续传至20代后用PCR、间接免疫荧光试验和免疫印迹试验进行检测,并绘制重组病毒体外生长曲线,分析其体外复制特性。结果表明,重组病毒rHVT-VP2能够稳定表达VP2蛋白,rHVT-VP2在CEF中的复制能力与亲本病毒无明显差异。重组病毒rHVT-VP2免疫鸡后能够诱导产生IBDV中和抗体,并对IBDV强毒株攻击引起的死亡提供90%免疫保护。重组病毒rHVT-VP2的构建为研制IBD重组HVT活载体疫苗奠定了基础,对IBD的防控具有重要意义。  相似文献   

12.
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV.  相似文献   

13.
传染性法氏囊病疫苗免疫雏鸡局部体液的免疫学变化   总被引：1，自引：0，他引：1  
应用间接ELISA法,检测了传染性法氏囊病疫苗免疫雏鸡4种局部体液(泪液、气管液、胆汁、肠液)免疫球蛋白IgG、IgM、IgA含量的动态变化。结果发现免疫雏鸡局部体液3种免疫球蛋白含量较未免疫对照雏鸡均有不同程度地升高;IBD超强毒株攻击后,对照雏鸡上述指标均明显低于疫苗免疫雏鸡,发生典型IBD,并全部死亡,而疫苗免疫雏鸡免疫保护率达60%,表明疫苗免疫后,可增强雏鸡的局部体液免疫功能。  相似文献   

14.
为制备外源病毒检验用抗鸡传染性法氏囊病病毒(Infectious Bursal Disease Virus,IBDV)的特异性血清,对300只3~4周龄的SPF鸡进行了基础免疫和加强免疫。最后一次免疫后21d采血并分离血清,血清经混合、分装、冷冻真空干燥后,对其进行了无菌检验、外源病毒检验、剩余水分测定、中和效价测定和特异性检验。结果表明,本研究制备的抗IBDV特异性血清无菌检验、外源病毒检验和剩余水分测定均符合《中华人民共和国兽药典》三部(二〇一〇年版)规定;血清中和效价为1:5747,与IBDV B87株毒种中和指数为104.3,与鸡新城疫病毒La Sota株、鸡传染性支气管炎病毒H120株、鸡传染性支气管炎病毒H52株、鸡传染性喉气管炎病毒、鸡痘病毒、禽呼肠孤病毒S1133株的中和指数均不大于10,通过AGP、ELISA、HI等试验确定血清中不含其他禽源外源病毒抗体。本研究为鸡传染性法氏囊病活疫苗或毒种的外源病毒检验提供了具有良好特异性和高效价的中和用血清。  相似文献   

15.
Wang X  Jiang P  Deen S  Wu J  Liu X  Xu J 《Avian diseases》2003,47(4):1305-1312
  相似文献   

16.
刘文利  高崧 《中国家禽》2004,8(Z1):52-56
选用两种在山东省普遍使用的中等毒力IBD疫苗,将试验分成12组,分别在12日龄和19日龄两次免疫商品鸡,同时在11日龄时用低聚异麦芽糖饮水,连用7天,二免后13天人工接种vvIBDV,连续观察14天。根据血清学检测指标,囊指数,BBIX值,病理组织学检查,临床保护率和病理保护率结果,都充分表明低聚异麦芽糖对传染性法氏囊病中等毒力疫苗具有显著的免疫增强作用,而且对提高法氏囊等免疫器官的保护作用及疫苗对其损伤后的修复作用也有显著效果。  相似文献   

17.
应用免疫SPA菌体花环法和间接ELISE法对传染性法氏囊病（IBD）疫苗免疫母鸡后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量进行了动态研究。结果发现：IBD疫苗免疫后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量均不同程度地高于未免疫的相应对照雏鸡，表明IBD疫苗免疫母鸡后，其子代雏鸡外周血液的体液免疫功能明显增强。而传染性法氏囊病超强毒株攻击子代雏鸡后，未免疫子代雏鸡外周血液的上述各项指标均明显低于疫苗免疫的子代雏鸡，这与IBDV感染雏鸡后，其体液免疫中枢器官法氏囊严重损害，淋巴细胞变性坏死等有关，也是导致感染雏鸡免疫抑制的基础。  相似文献   

18.
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-ã was constructed. Twice at 2-week intervals, two-week-old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2 after the second immunization, the chickens were orally challenged with the highly virulent IBDV. The groups that received the DNA vaccines plus either DDA or CpG-ODN showed significantly lower survival rates than the group that received the DNA vaccine alone. However, the survival rates for the DNA vaccine alone and for the DNA vaccine plus ChIFN-γ were similar. The chickens had no detectable antibodies to the IBDV before the challenge but all the surviving chickens in all groups except for the normal control group showed the induction of antibodies to the IBDV at day 10 after the challenge. As judged by the lymphocyte proliferation assays using the a WST-8 solution performed on the peripheral blood and splenic lymphocytes, the stimulation indices (SI) of the peripheral blood lymphocytes in all groups except for the normal control group were similar immediately before the challenge. At 10 days post-challenge, the SI for DNA vaccine plus either CpG-ODN or ChIFN-γ was similar to that of the DNA vaccine control group. For splenic lymphocytes, the SI in the DNA vaccine plus CpG-ODN and DNA vaccine plus ChIFN-γ groups were higher than for the DNA vaccine control. These results suggest that DDA actually compromises the protection against the IBDV by DNA vaccine, and CpG-ODN and IFN-γ had no significant effect.  相似文献   

19.
J M Sharma 《Avian diseases》1985,29(4):1155-1169
Studies with specific-pathogen-free chickens revealed that chicks hatching from eggs inoculated at the 18th day of embryonation with infectious bursal disease (IBD) vaccine viruses of low virulence (isolates TC-IBDV and BVM-IBDV) developed antibody against IBD virus (IBDV) and resisted challenge with virulent IBDV at 3 weeks of age or older. Embryo vaccination did not adversely affect hatchability of chicks or survival of hatched chicks. Chicks embryonally vaccinated with TC-IBDV had transient histologic lesions in the bursa of Fabricius at hatch. Similar but milder lesions were also noted in chickens that received TC-IBDV at hatch. The level of protection following embryo vaccination with TC-IBDV and BVM-IBDV was similar to that following vaccination with the same vaccines at hatch. Vaccine viruses of moderate virulence (isolates BV-IBDV and 2512-IBDV) were not suitable as vaccines in embryos lacking maternal antibody to IBDV, because the vaccinated chicks developed acute IBD after hatch. Isolate 2512-IBDV was not pathogenic for embryos bearing maternal antibody to IBDV. Maternal antibody against IBDV interfered with efficacy of embryo vaccination with BVM-IBDV but not with 2512-IBDV. Embryo vaccination with a mixture of vaccines against IBD and Marek's disease resulted in protection of hatched chicks against challenge with virulent IBDV and Marek's disease virus.  相似文献   

20.
Infectious bursal disease (IBD) is still a significant threat facing the Egyptian poultry industry. In this study, eight very virulent IBD viruses (vvIBDV), originating from acute IBD outbreaks recorded in lower and upper Egypt, were studied with respect to their ability to replicate in cell culture, antigenicity and immunogenicity of classic vaccine. Six continuous cell lines and one primary cell culture were tested for replication of the vvIBDVs. None of the vvIBDV isolates could be adapted to BGM-70, Vero, BHK, RK-13 or MDBK cell lines or chicken embryo fibroblast cells after six blind passages. Serological typing with three neutralizing monoclonal antibodies showed antigenic variation among vvIBDVs. The immunogenicity induced by graded doses of classic-intermediate vaccine in both IBD-resistant (Mandarah) and susceptible (Gimmizah) Egyptian chickens was investigated. The protection of the tested doses was evaluated by measurement of the serological response and resistance to vvIBDV challenge 10 days post vaccination. Similar antibody responses to the vaccine were generated over a wide (100-fold) dose range. It was concluded that single vaccination, by eye drops, with classic-intermediate vaccine (1 x) could protect chickens against clinical disease and mortality. However, the immune responses generated by 1 x, 10 x or 100 x vaccine doses did not protect against bursal damage following challenge. This finding points to the highly invasive nature of the prevailing vvIBDVs in Egypt.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号