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该文对亚洲分布的E .barbeyanum ,E .dipsacoides,E .cristatum ,E .echinulatum ,E .hamiltonianum ,E .henryanum ,E .hookerianum ,E .kradungense,E .nautiliforme ,E .nigrum和E .thwaitesii等谷精草属 (EriocaulonL .) 11个种的分类问题进行了详细的研究 .补充并纠正了E .nigrumLec .和E .halmitoniamumMartius的形态描写 .公开发表新种E .pseudotruncatum和新变种E .truncatumHam .exMartiusvar.florensense.E .seticuspeOhwi,E .echinulatumMartiusvar.seticuspeOhwi,E .echinulatumMartiusvar.tenueSatake ,E .robinsoniiMoldenke和E .poilaneiMoldenke 5个种 (变种 )处理为同物异名 相似文献
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该文对亚洲分布的E.barbeyanum,E.dipsacoides,E.cristatum,E.echinulatum,E.hamiltonianum,E.henryanum,E.hookerianum,E.kradungense,E.nautiliforme,E.nigrum和E.thwaitesii等谷精草属(Eriocaulon L.)11个种的分类问题进行了详细的研究.补充并纠正了E.nigrum Lec.和E.halmitoniamum Martius的形态描写.公开发表新种E.pseudotruncatum和新变种E.truncatum Ham.ex Martius var.florensense.E.seticuspe Ohwi,E.echinulatum Martius var.seticuspe Ohwi,E.echinulatum Martius var.tenue Satake,E.robinsonii Moldenke和E.poilanei Moldenke 5个种(变种)处理为同物异名. 相似文献
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安徽省薹草属(Carex Linn.)植物增补 总被引：2，自引：0，他引：2
苏宋旺 《安徽农业大学学报》2009,36(4)
报道采自安徽的薹草属3个新种、2个亚种和1个变种以及薹草属1个新组.即牯牛薹草Carex guniuensis S.W.Su,琅琊薹草Carex langyaensis S.W.Su,X.M.Fang,et al,缘喙薹草Carex rhynchophora Franch.var.margineorostris S.W.Su,大通薹草Carex datongensis S.W.Su,华阳薹草Carex truncatigluma C.B.Clarke subsp.huayangensis S.W.Su,长穗刻鳞薹草Carex incisa Boott subsp.longissima S.W.Su;1个新组:隐匿薹草组Sect.Infos-sae S.W.Su.Sect.nov. 相似文献
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亚洲谷精草属植物研究(Ⅰ) 总被引：2，自引：1，他引：1
讨论谷精草属 (Eriocaulon L.) 世界性及亚洲分布的E. setaceum, E. australe, E. exangulare, E.willdenovianum, E. decemflorum, E.wightianum, E.cinereum, E. alpestre 8个种的分类问题. 补充纠正了E. alpestre Koern. 的形态描写.10个种(变种)处理为同物异名. 相似文献
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Ning SUN Sanfeng CHEN Xiangming XIE Yueju WANG Gangqiang LI Nan WANG Dehu LIU 《农业科学与技术》2014,(3):321-325
T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophiliza-tion. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cel wal of Xan-thomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb. nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. mal-vacearum, Fusarium oxysporium sp. vasinfectum, Verticil ium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasin-fectum and V. d. kleb were also analyzed. 相似文献
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桉树伞房属4个种在广东清新的早期生长表现 总被引：2，自引：1，他引：1
分析了引自澳大利亚东部的桉树伞房属4个种在广东省清新试验点5年生时的生长表现.方差分析表明,树种间、种源间、家系间在树高、胸径、材积生长量上均存在极显著的差异.表现最好的是大叶斑皮桉Corymbia henryi (S.T. Blake) K.D. Hill & L.A.S. Johnson,其次是斑皮柠檬桉C. variegata (F. Muell.) K.D. Hill & L.A.S. Johnson、柠檬桉C. citriodora (Hook.) K.D. Hill & L.A.S. Johnson和斑皮桉C. maculata (Hook.) K.D. Hill & L.A.S. Johnson.4个树种的平均树高为8.04 m,平均胸径为7.28 cm,平均单株材积为0.021 9 m3.其中斑皮柠檬桉10248种源44号家系的生长量最大,其平均树高为10.35 m,平均胸径为9.70 cm,平均单株材积为0.040 00 m3.初步选出20个家系,占家系总数的21.98%. 相似文献
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ZONG Xu-xiao Rebecca Ford Robert R Redden GUAN Jian-ping WANG Shu-min 《中国农业科学(英文版)》2009,8(3):257-267
To assesse the genetic diversity among wild and cultivated accessions of 8 taxonomic groups in 2 species, and 5 subspecies under Pisum genus, and to analyze population structure and their genetic relationships among various groups of taxonomy, the study tried to verify the fitness of traditionally botanical taxonomic system under Pisum genus and to provide essential information for the exploration and utilization of wild relatives of pea genetic resources. 197 Pisum accessions from 62 counties of 5 continents were employed for SSR analysis using 21 polymorphic primer pairs in this study. Except for cultivated field pea Pisum sativum ssp. sativum var. sativum （94 genotypes）, also included were wild relative genotypes that were classified as belonging to P. fulvum, P. sativum ssp.abyssinicum, P. sativum ssp. asiaticum, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. elatius, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. arvense （103 genotypes）. The PCA analyses and 3-dimension PCA graphs were conducted and drawn by NTSYSpc 2.2d statistical package. Nei78 genetic distances among groups of genetic resources were calculated, and cluster analysis using UPGMA method was carried out by using Popgene V1.32 statistical package, the dendrogram was drawn by MEGA3.1 statistical package. Allelic statistics were carried out by Popgene V1.32. The significance test between groups of genotypes was carried out by Fstat V2.9.3.2 statistical package. 104 polymorphic bands were amplified using 21 SSR primer pairs with unambiguous unique polymorphic bands. 4.95 alleles were detected by each SSR primer pair in average, of which 65.56% were effective alleles for diversity. PSAD270, PSAC58, PSAA18, PSAC75, PSAA175 and PSAB72 were the most effective SSR pairs. SSR alleles were uniformly distributed among botanical taxon units under Pisum genus, but significant difference appeared in most pairwise comparisons for genetic diversity between taxon unit based groups of genetic resources. Genetic diversity level of wild species P. fulvum was much lower than the cultivated species P. sativum. Under species P. sativum, P. sativum ssp. sativum var. sativum and P. sativum ssp. asiaticum were the highest in gentic diversity, followed by P. sativum ssp. elatius var. elatius and P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio, P. sativum ssp. sativum vat. arvense and P. sativum ssp. abyssinicum were the lowest. Four gene pool clusters were detected under Pisum genus by using PCA analysis. Gene pool ＂fulvum＂ mainly consisted of wild species Pisum fulvum, gene pool ＂abyssinicum＂ mainly consisted of P. sativum ssp. abyssinicum, and gene pool ＂arvense＂ mainly consisted of P. sativum ssp. sativum var. arvense. While gene pool ＂sativum＂ were composed by 5 botanical taxon units, they are P. sativum ssp. asiaticum, P. sativum ssp. elatius var. elatius, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. sativum. ＂sativum＂ gene pool constructed the primary gene pool of cultivated genetic resources; ＂fulvum＂ gene pool, ＂abyssinicum＂ gene pool and ＂arvense＂ gene pool together constructed the secondary gene pool of cultivated genetic resources. Pairwise Nei78 genetic distance among botanical taxon based groups of pea genetic resources ranged from 7.531 to 35.956, 3 large cluster groups were identified based on the UPGMA dendrogram. Group Ⅰ equals to ＂sativum＂ and ＂arvense＂ gene pools, Group Ⅱ equals to ＂abyssinicum＂ gene pool, and Group Ⅲ equals to ＂fulvum＂ gene pool. The UPGMA clustering results generally supporting the PCA clusting results. There were significant differences among most botanical groups under Pisum genus, with clear separation of four gene pools for genetic diversity structure. The research results partially support the traditional botanical taxonomy under Pisum genus, and pointed out its advantage and shortcoming. In order to broaden the genetic bases of pea varieties, the genetic potentials in the four gene pools should be thoroughly exploited. 相似文献
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The phylogenic and genetic relationships in germplasm resources of Chinese Ziziphus were studied at molecular levels, for providing new molecular evidences of classification, protection, and utilization of germplasm resources of Ziziphus. The sequence-related amplified polymorphism （SRAP） was assessed to analyse the genetic relationships among 14 species of Ziziphus, 11 cultivars of Z. jujuba Mill., and one outgroup. A total of 580 DNA bands were amplified by 19 selective primers, 570 of which （98.28%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 30. The genetic similarities of 26 sample materials were between 0.22 and 0.99. UPGMA method cluster analysis showed that 26 sample materials were classified into six cluster groups with the genetic similarity of 0.28. The results showed that SRAP technique is efficient in studying genetic relationships among Ziziphus, Z. jujuba Mill. and Z. acidojujuba C. Y. Cheng et M. J. Liu which should be treated as one species, and further infraspecific classification of Z. jujuba Mill. should be classified into two subspecies; the scientific names of new taxa, Chinese jujube, and wild jujube were Ziziphus jujuba Mill. subsp, jujuba and Ziziphus jujuba Mill. subsp, spinosa （Bunge） J. Y. Peng, X. Y. Li et L. Li, respectively. Z. xiangchengensis Y. L.Chen et P. K. Chou, Z. montana W.W. Smith and Z. mairei Dode might be originated from same ancestry. Z xiangchengensis Y. L. Chen et P. K. Chou and Z. montana W.W. Smith should be treated as one species. The infrasubspecific taxon of Z. jujuba Mill. was not suitable to set up varieties. 相似文献
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亚洲谷精草属植物研究(I) 总被引：5，自引：5，他引：0
讨论谷精草属 (EriocaulonL .)世界性及亚洲分布的E .setaceum ,E .australe,E .exangulare,E .willde novianum ,E .decemflorum ,E .wightianum ,E .cinereum ,E .alpestre 8个种的分类问题 .补充纠正了E .alpestreKoern .的形态描写 .10个种 (变种 )处理为同物异名 . 相似文献
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猪繁殖与呼吸综合症免疫学研究 总被引：16，自引：1，他引：16
猪繁殖与呼吸综合症是由猪繁殖与呼吸综合症病毒引起的高度传染性病毒病。笔者就呼吸综合症病毒的理化特性、生物学特征、基因组构成、病毒复制、免疫学特征以及疫苗等作一简述 相似文献
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高致病性猪繁殖与呼吸综合征（PRRS）俗称蓝耳病,是由猪繁殖和呼吸综合征病毒变异株引起的一种急性高致死性疫病,该病是一种免疫抑制病,其感染宿主唯一,且常常继发其他病原感染。PRRS发病时间间隔为2～37d,一般为5d左右,任何年龄、品种猪只均可发病。猪对很多PRRSV的感染途径敏感,包括口腔、鼻腔、肌肉、腹腔和生殖道。该病传染性高,并且只要有10个或者更少的病毒粒子,就可发生PRRS。疫苗免疫是预防PRRS的重要手段之一,目前有灭活疫苗和弱毒疫苗可供使用。针对高致病性猪蓝耳病的有效防控研究正在许多科研机构深入开展,能引起猪体对PRRSV产生保护性抗体的免疫原性基因的筛选、改造和利用等相关研究正加紧进行,以期研制一种安全高效的PRRS疫苗。 相似文献
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3种疫苗单独和联合免疫对猪抗体水平的影响 总被引：1，自引：0，他引：1
{目的]寻求有效结合注射的新型免疫程序,以有效控制猪瘟（CSF）、猪口蹄疫（FMD）和猪繁殖与呼吸综合征（PRRs）疫情的发生和流行[方法]在陕西省横山和米脂两县将140头猪随机分为7组,每组20头,采用CSF活疫苗（脾淋源）、FMD灭活疫苗（0型Ⅱ）、PRRS灭活疫苗（NVDC—JXA1株）分别进行单独、2种及3种同时分．点免疫注射试验,用正向间接血凝和EUSA法检测猪抗体水平。l结果10）3种疫苗都可以刺激猪产生特异性抗体,并且抗体在21、28和35d呈规律性增长。②CSF疫苗与FMD、PRRS疫苗分开和三者同时使用时．虽然FMD、PRRS疫苗推迟CSF抗体的上升时问,但是对CSF的免疫效果产生的影响不明显。（3）PRRS与FMD疫苗同时注射后,所产生抗体效价在21、28和35d均比单独注射PRRS疫苗阳性率高,说明FMD疫苗可对PRRs疫苗注射后有效抗体的产生达到积极协同作用,促进PRRS有效免疫抗体的产生,同时免疫CSF疫苗和PRRS疫苗的抗体水平低于单独注射PRRS疫苗,说明共同免疫CSF和PRRS疫苗时,2种疫苗会相互影响,使免疫效果不如单独免疫。④FMD和PRRS疫苗同时注射后,FMD疫苗所产生的抗体水平（OD值）最高,且一直呈上升趋势,优于FMD疫苗单独注射所产生的抗体水平,说明FMD和PRRS疫苗同时注射,更能促进FMD抗体的产生．[结论]3种疫苗同时分点注射和单注CSF产生抗体后再同时分点注射FMD和PRRS都是较好的防疫体系,具体应根据养殖规模及地域选择合适的防疫体系. 相似文献
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猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是由PRRS病毒(PRRSV)引起的以母猪繁殖障碍和仔猪呼吸困难、病毒血症为典型症状的一种传染性疾病,给世界养猪业带来巨大的经济损失。深入研究PRRSV的相关致病机制可为该病防控奠定理论依据。在对PRRSV的研究中,非结构蛋白2(non-structural protein 2, Nsp2)一直是研究的热点,文章综述了Nsp2与遗传变异、病毒致病性、病毒复制、免疫调控和重组疫苗与分子标签等相关的研究进展。Nsp2基因序列在对疫苗毒株的鉴别诊断中发挥重要作用。 相似文献
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间接血凝试验检测猪繁殖与呼吸综合征病毒抗体 总被引：3，自引：0，他引：3
以猪繁殖与呼吸综合征（PRRS）病毒致敏猪红细胞作诊断液,用间接血凝试验（IHA）检测猪繁殖与呼吸综合征病毒抗体。检测了可疑猪场80份血清样本,PRRS阳性32份,阳性率40%,与ELISA试验比较符合率98%。用此方法检测PRRS疫苗免疫猪血清,发现其抗体水平在初免后15天升高,二免后15天达最高峰,二免后45天抗体水平消失。 相似文献
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为提高目前高致病性蓝耳病（HR—PRRS）灭活疫苗的免疫效果，将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍，再经灭活后用Sephamse 4 Fast Flow分子筛柱层析，收集完整的病毒粒子，加油佐剂制成纯化HP—PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到415以上，血清抗体检测阳转率达86％以上，均高于同批病毒液制成的未纯化灭活疫苗，且差异显著。试验证明，HR—PRRS病毒液经浓缩纯化制成疫苗后，增强了免疫效果。 相似文献
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