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1.
侵染南瓜的西瓜花叶病毒和黄瓜花叶病毒CP基因的克隆和序列分析 总被引：1，自引：0，他引：1
采用RT-PCR的方法,用马铃薯Y病毒属病毒3′-末端序列的简并引物和黄瓜花叶病毒（Cucumber mosaic virus, CMV）外壳蛋白（CP）基因的特异引物,对采自山东聊城的一表现花叶、黄化、蕨叶及果实畸形的南瓜样品进行了检测,同时扩增到了西瓜花叶病毒（Watermelon mosaic virus, WMV）和CMV 2种病毒的基因组片段,说明该样品受到WMV和CMV 2种病毒的复合侵染。这2个病毒分离物分别被命名为WMV-liaocheng和CMV-liaocheng。序列测定及分析结果表明,它们与其它相应病毒分离物CP基因核苷酸序列的同源性分别为91.2-98.0%和77.0-97.9%,推导的氨基酸序列同源性分别为96.4-98.5%和81.2-99.1%。根据完整CP 基因核苷酸序列构建的系统进化树显示：18个WMV分离物可分为3组,其中WMV-liaocheng 与HLJ、CHN及Habenaria等分离物表现出较近的亲缘关系,形成Ⅲ组;30个CMV分为两个亚组,其中CMV-liaocheng属于亚组I,CMV-liaocheng可能发生过重组。 相似文献
2.
研究旨在通过逆转录聚合酶链式反应（RT-PCR）从分子方面鉴定附子地道产区四川江油乌头花叶病植株的病原物种类及分析其特性。通过对黄瓜花叶病毒（Cucumber mosaic virus, CMV）的外壳蛋白基因(Coat Protein,CP)设计特异引物,对江油中坝附子GAP生产基地的乌头花叶病植株进行克隆、转化并测序。结果表明：从江油花叶病乌头植株中克隆得到基因大小为375 bp的片段,鉴定为黄瓜花叶病毒,命名为CMV-FZ,该序列为一段有效的长为375 nt的氨基酸编码区,推测编码125个氨基酸残基。依据外壳蛋白核苷酸序列建立了CMV不同分离物的系统进化树并对该序列进行蛋白组学分析,CMV-FZ与国内外已报道代表性CMV亚组分离物CP基因的核苷酸与氨基酸的序列相似性为74.8%~98.2%和73.4%~99.2%,其中与与CMV亚组II的核苷酸和氨基酸序列相似性为96.1%-98.2%和96.0%-99.2%,该分离物与中国分离物（CMV-PCT2）氨基酸序列相似性最高,达到99.2%。遗传进化分析显示,CMV-FZ与中国分离物（Zin和Hubei-1）和美国分离物（DKRD）亲缘关系较近,聚在同一分支,属于CMV亚组II。以上结果表明伴有花叶症状的乌头受到黄瓜花叶病毒的侵染。 相似文献
4.
山东葫芦科蔬菜上病毒病种类检测及黄瓜花叶病毒分离物的亚组鉴定 总被引：1，自引：0，他引：1
为了检测和鉴定山东地区葫芦科蔬菜上病毒病种类,从山东诸地11个蔬菜种植区采集了867个葫芦科蔬菜疑似感病样品。利用黄瓜花叶病毒、黄瓜绿斑驳花叶病毒、西瓜花叶病毒、烟草花叶病毒、小西葫芦黄花叶病毒、南瓜花叶病毒、甜瓜黄斑病毒、瓜类褪绿黄化病毒、李属坏死环斑病毒及甜瓜坏死斑点病毒等特异性引物对疑似感病样品分别进行RT-PCR检测,各病毒检出率分别为34.8%,10.4%,20.0%,41.7%,27.0%,7.8%,2.6%,1.7%,0,0.9%。表明除PNRSV外,其他9种病毒在山东各地均有发生,且CMV和TMV发病率较高,2种或2种以上病毒复合侵染也很普遍。同时为明确山东地区CMV分离物的株系分化状况,选取11个地区有代表性的CMV阳性样品,测定其外壳蛋白(Coat protein,CP)和RNA3核苷酸序列并进行序列比对和进化分析,结果显示侵染山东地区葫芦科蔬菜的CMV分离物均属于IB亚组,与韩国分离物As(AF013291)相似性最高,未发现其他株系。 相似文献
5.
《华北农学报》2021,36(5)
为了明确引起天津地区番茄果实褐化的病因,采用小RNA深度测序技术和RT-PCR技术对采自天津的番茄病样进行鉴定分析。结果表明,引起天津地区番茄果实变褐的病原为番茄花叶病毒。进一步对该病毒进行了全基因组测序,并对该病毒的外壳蛋白(CP)、运动蛋白(MP)和126蛋白进行了序列分析。进化树分析结果表明,天津分离物的3个蛋白基因均与美国分离物USA_99-1)的进化关系最近。相似性分析结果表明,CP基因与其他分离物的平均相似性为95.53%。MP基因与其他基因分离物的平均相似性为95.21%。126蛋白基因与其他分离物的平均相似性为92.72%。对这3个基因编码的蛋白进行分析,结果表明,不同分离物之间的平均相似性均在99.0%及以上。初步表明,在天津发生的番茄花叶病毒可能是由于贸易活动由国外传入天津市。 相似文献
6.
烟草花叶病毒南瓜分离物CP基因的克隆、序列分析及其原核表达 总被引：2，自引：0，他引：2
为从分子水平鉴定山东聊城的一表现明显花叶、黄化、蕨叶及果实畸形的南瓜病毒病的病原.采用RT-PCR的方法,用TMV外壳蛋白(CP)基因的特异引物,对该样品进行了检测,克隆到的基因序列进行分析,并使其在大肠杆菌中表达.结果表明,从该样品中扩增到了TMV的CP基因,说明该样品受到TMV的侵染,首次发现TMV自然侵染南瓜.对CP基因序列测定及分析表明,与GenBank上其他TMV分离物CP基因核苷酸序列的同源性为86.5%～99.0%,推导的氨基酸序列同源性为93.7%～99.4%.根据完整CP 基因核苷酸序列构建的系统进化树显示:32个TMV分离物可分为5个组,其中TMV-liaocheng与Nakron Pathom、Fujian、017等分离物属于Ⅳ组.TMV-liaocheng可能发生过重组.将TMV-liaocheng CP基因与原核表达载体pET-22b(+)连接,在大肠杆菌BL21(DE3)pLysS诱导表达出分子量约20 kDa的融合蛋白,并用Ni2+ -NTA His · Bind(R)树脂纯化,纯化后的蛋白可直接用于制备特异性的抗血清,为准确、快速地检测TMV奠定了基础. 相似文献
7.
对2021年发生在银川、徐州、赤峰的番茄叶片出现黄绿相间的斑驳、叶片皱缩卷曲、植株矮化等疑似番茄病毒病的症状进行了鉴定。利用12种番茄病毒病的特异性引物进行RT-PCR检测，发现所检测的不同地区样品中，侵染番茄最多的病毒为黄瓜花叶病毒（CMV），检出率为60.00%，其次是番茄斑萎病毒（TSWV），检出率为46.67%。通过核苷酸序列比对发现，检测出的这2种病毒与CMV(AJ131619.1)和TSWV(MK318839.1)同源性最高，相似度分别为97%和99%。同时，基于CMV和TSWV基因序列构建系统发育树表明，宁夏银川地区番茄病毒样本为CMV和TSWV复合侵染。 相似文献
8.
葡萄A病毒外壳蛋白基因克隆及分子变异分析 总被引：1，自引：0，他引：1
为获得葡萄A病毒(Grapevine virus A,GVA)外壳蛋白(CP)基因,并为开展GVA-CP介导的抗病毒转基因研究提供基础,本研究从葡萄休眠枝条中扩增获得12个GVA分离物的CP基因序列,全长597nt,编码198个氨基酸。所得分离物间核苷酸和氨基酸序列同源性分别为83.9%~99.8%和92.4%~99.5%,与GenBank中12个GVA CP序列比较,核苷酸和氨基酸序列同源性分别为71.0%~93.1%和81.8%~98.0%。系统进化分析表明,本研究12个分离物CP序列与已报道的GVA Ⅰ组亲缘关系最近。种群变异分析表明,GVA种群具有多种变异体类型,并且多个样品存在不同变异体类型的复合侵染。但绝大多数变异体克隆聚集在GVA Ⅰ组分支,有60%的克隆序列间同源性较高,并且聚集在同一个次级分支Ⅰ Aa,表明其为该GVA种群的优势序列。克隆的GVA CP基因以及优势序列群体的分析为葡萄抗病毒转基因研究奠定了基础。 相似文献
9.
为了明确引起北京地区草莓病毒病的病毒种类和病毒序列的分子变异特点,从北京地区表现畸形症状的草莓叶片中提取总RNA,利用RT-PCR技术扩增得到草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)的含有外壳蛋白(CP)基因的729 nt特异性核苷酸片段。经序列测定和分析可知,北京地区存在2种不同的SMYEV分离物（BJ1和BJ2）,两者间的核苷酸序列同源性仅为82.72%~82.96%。将北京分离物与分别来自德国、美国、智利、捷克、澳大利亚、比利时、意大利、韩国和中国沈阳等国家或地区的不同分离物的CP基因序列进行分析比较。结果显示,33个分离物可以分为四大组群,BJI和BJ2分别归属不同组群,BJ1与19个其他国家的分离物亲缘关系较近,BJ2与中国沈阳的2个分离物亲缘关系较近。BJ1和BJ2与其他分离物的CP基因核苷酸序列同源性分别为81.10%~98.35%和82.03%~98.63%,氨基酸序列同源性介于82.17%~99.17%和90.91%~94.63%。 相似文献
10.
天津地区番茄褪绿病毒的分子检测与基因组部分序列分析 总被引：1，自引：0，他引：1
为了确定天津番茄产区是否发生了番茄褪绿病毒病,了解该病毒天津分离物的主要基因是否发生变异,对该地区发生的To CV进行了分子检测,并对该病毒的外壳蛋白(CP)和热激蛋白70类似物(Hsp70h)的核苷酸和氨基酸进行了序列分析。结果表明,天津分离物CP蛋白的核苷酸和氨基酸序列与已报道的具有代表性的不同国家的分离物相似性均在97.3%以上。Hsp70h蛋白的核酸和氨基酸序列与已报道的具有代表性的不同国家的分离物相似性均在97.2%以上。表明CP和Hsp70h蛋白是2个最保守的蛋白。这是首次在分子水平上证明番茄褪绿病毒在天津地区为害。 相似文献
11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
13.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
15.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
16.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
17.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf
1) and recessive for the other (Rf
2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph
3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf
1(one of two dominant additive genes controlling early season leafing out) indicated that Rf
1and Rf
2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf
1, and 0.15 for Lf
1-Sph
3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph
3.It is suggested that competitive disadvantage of lf
1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph
3. Poor performance of lf
1- (and possibly lf
2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off). 相似文献
18.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho... 相似文献
19.
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed. 相似文献
20.
Parasitic angiosperms cause great losses in many important crops under different climatic conditions and soil types. The most widespread and important parasitic angiosperms belong to the genera Orobanche, Striga, and Cuscuta. The most important economical hosts belong to the Poaceae, Asteraceae, Solanaceae, Cucurbitaceae, and Fabaceae. Although some resistant cultivars have been identified in several crops, great gaps exist in our knowledge of the parasites and the genetic basis of the resistance, as well as the availability of in vitro screening techniques. Screening techniques are based on reactions of the host root or foliage. In vitro or greenhouse screening methods based on the reaction of root and/or foliar tissues are usually superior to field screenings and can be used with many species. To utilize them in plant breeding, it is necessary to demonstrate a strong correlation between in vitro and field data. The correlation should be calculated for every environment in which selection is practiced. Using biochemical analysis as a screening technique has had limited success. The reason seems to be the complex host-parasite interactions which lead to germination, rhizotropism, infection, and growth of the parasite. Germination results from chemicals produced by the host. Resistance is only available in a small group of crops. Resistance has been found in cultivated, primitive and wild forms, depending on the specific host-parasite system. An additional problem is the existence of pathotypes in the parasites. Inheritance of host resistance is usually polygenic and its transfer is slow and tedious. Molecular techniques have yet to be used to locate resistance to parasitic angiosperms. While intensifying the search for genes that control resistance to specific parasitic angiosperms, the best strategy to screen for resistance is to improve the already existing in vitro or greenhouse screening techniques. 相似文献