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1.
本试验对56头娟姗牛与179头荷斯坦青年母牛进行超排处理,分别获得可用胚胎227枚和1242枚;可用胚率分别为57.80%和70.65%,娟姗牛和荷斯坦牛超排黄体数无明显差异(P>0.05),但采胚数明显低于荷斯坦牛,娟姗牛的超排结果明显低于荷斯坦牛的结果(P<0.05),移植受体牛270头,怀孕157头。移植妊娠率分别达到52.54%和50.20%,同期娟姗牛胚胎与荷斯坦牛胚胎移植结果比较,无显著差异(P>0.05)。娟姗牛超排使用FSH剂量为9mg更为经济。青年荷斯坦牛和娟姗牛可用于胚胎生产,但青年娟姗牛的可用胚胎数低于荷斯坦牛。  相似文献   

2.
为探讨荷斯坦奶牛连续超数排卵的效果,对8头供体以30d为间隔连续超排3次,3次超排有效率分别为:100(8/8)、100(8/8)和100(8/8),3次头均荻胚数分别为:10.63±3.18枚、10.13±1.78枚和10.38±1.58枚,3次头均可用胚数分别为:7.13±1.29枚、6.38±0.92枚和5.88±1.17枚。3次超排的头均荻胚数间和头均可用胚数间差异不显著。结果表明,荷斯坦奶牛能够以30d为间隔进行连续超排。  相似文献   

3.
本试验比较了不同剂量的促卵泡素对高产荷斯坦奶牛超排效果的影响,试验分2组,利用FSH减量法对供体荷斯坦奶牛进行超数排卵。A组FSH剂量为13mg/头,B组为12mg/头。试验结果A组平均黄体数13.5个,平均获可用胚数9.5枚,均高于B组的平均黄体数3个和平均获可用胚数2.5枚。试验结果初步表明:应用13mgFSH组的超排效果好于12mg组。  相似文献   

4.
促排卵3号在荷斯坦供体母牛超排中的应用效果   总被引:1,自引:1,他引:0  
采用CIDR+FSH+PG法对114头进口新西兰荷斯坦供体母牛进行超排。其中LRH—A3处理60头,共回收卵651枚,回收率95.0%(651/685),可用胚362枚,头均6.0枚,可用率56.0%;对照组54头未用LRH—A3处理,共回收卵526枚,回收率93.8%(526/561),可用胚总数250枚,头均4.6枚,可用率47.6%。结果表明,LRH—A3对荷斯坦母牛超数排卵有显著影响。  相似文献   

5.
用两种不同剂量的FSH进行超排处理 ,结果表明 ,用 9mg的FSH处理 ,头均获得胚胎 6 2枚、可用胚 3 8枚 ;分别比 8mg处理组多 3 4枚、1 8枚 (P <0 0 5 )。表明用 9mgFSH进行超排处理效果较好。  相似文献   

6.
试验旨在探索优化超排方案,提高肉羊胚胎生产效率。在新疆哈密市牧祥合作社用FSH+PG递减4 d 8针注射法处理供体羊44只,冲胚数503枚、只均冲胚数11.43枚±0.79枚,可用胚数394枚、只均可用胚数8.95枚±0.95枚;同样的方法用FSH+PG+PMSG处理供体45只,冲胚数608枚、只均冲胚数13.51枚±1.04枚,可用胚数435枚、只均可用胚数9.67枚±1.06枚,差异均极显著(P0.01);对39只供体羊分别采用开膣器输精+自然交配+开膣器输精和自然本交方法配种,重复超排跟踪统计,重复超排和不同授精方式冲胚数无明显差异(P0.05);选用自然发情与人工诱情法处理,自然发情组可用胚胎数289枚,诱情组可用胚胎数221枚,两者差异极显著(P0.01);供体羊在不同营养状态下超排处理,营养良好者与营养中等者只均回收胚胎数分别为14.4枚和8.52枚,只均可用胚胎平均数分别为7.0枚和5.25枚,差异也极显著(P0.01)。该研究优化了绵羊超排方法和配种授精方案,初步建立了高效胚胎移植体系。  相似文献   

7.
青年荷斯坦牛的超数排卵试验   总被引:4,自引:0,他引:4  
采用不同剂量的FSH PG的超排处理方法对 75头澳大利亚牛分别在冬、春季进行超数排卵试验。结果显示 :春季的超排反应率比冬季提高 4 77个百分点 ,春季的直检黄体数、回收卵数和可用胚数分别增加 4 2 1(P <0 0 1)、4 5 3 (P <0 0 1)和 3 13 (P <0 0 5 )枚 ,可用胚率则减少 2 3 2个百分点 ;10mgFSH组的超排反应率比 7 5mgFSH组提高 3 7个百分点 ,直检黄体数、回收卵数和可用胚数分别增加 4 71(P <0 0 1)、5 0 9(P <0 0 1)、3 78(P <0 0 1)枚 ,可用胚率增加 0 3 1个百分点 ;在供体牛发情周期的第 812天对其进行超排处理 ,结果直检黄体数、回收卵数、可用胚、可用胚率均无显著差异 ,但 910天的直检黄体数、回收卵数和可用胚数量最多。  相似文献   

8.
青年荷斯坦牛的超数排卵试验   总被引:1,自引:0,他引:1  
采用不同剂量的FSH +PG的超排处理方法 ,分别在冬、春季对 75头澳大利亚牛进行超数排卵试验。结果显示 :春季的超排反应率比冬季提高了 4 .77个百分点 ,春季的直检黄体数、回收卵数和可用胚数分别增加 4 .2 1枚 (P <0 .0 1)、4 .5 3枚 (P<0 .0 1)和 3.13枚 ,(P <0 .0 5 )可用胚率则减少了 2 .32个百分点 ;10 .0mgFSH组的超排反应率比 7.5mgFSH组提高了 3.70个百分点 ,直检黄体数、回收卵数和可用胚数分别增加了 4 .71枚 (P <0 .0 1)、5 .0 9枚 (P <0 .0 1)和 3.78枚 ,(P<0 .0 1)可用胚率增加了 0 .31个百分点 ;在供体牛发情周期的第 8~ 12天对其进行超排处理 ,结果直检黄体数、回收卵数、可用胚和可用胚率均无显著差异 ,但 9~ 10d的直检黄体数、回收卵数和可用胚数量最多。  相似文献   

9.
应用X精子冷冻精液生产体内性控胚胎的试验   总被引:1,自引:1,他引:0  
采用CIDR FSH PG法对206头优秀荷斯坦供体母牛进行超数排卵,其中实验组73头超排后用X性控冷冻精液输精(每头3支),获可用胚310枚,头均可用胚4.25枚;对照组133头,采用常规冷冻精液输精(每头3支),获得可用胚787枚,头均可用胚5.92枚,差异不显著。结果表明,用已分离的X精子冷冻精液对超排母牛进行人工授精生产体内性控胚胎是可行的。  相似文献   

10.
对79只波尔山羊采用不同制剂FSH进行超排处理。结果:①宁波产FSH在38Iu、42Iu剂量下所获得的平均可用胚胎数(12.39±3.31)枚(38Iu)、(13.06±4.26)枚(42Iu)无显著差异(P>0.05)。②进口FSH在8mL、10mL剂量下获得的平均可用胚胎数(18.35±4.45)枚、(17.40±5.12)枚无显著差异(P>0.05)。③用进口FSH超排处理时所获得的平均排卵数、采集胚胎数、采集的可用胚胎数均显著高于国产FSH(P<0.05)。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

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