首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到19条相似文献，搜索用时 203 毫秒
1.
[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.  相似文献   

2.
Soybean Dongnong 42 from northeast of China was used as an experimental material. The samples were tested by spraying different concentrations of LaCl3, CeCl3 and LaCl3+CeCl3 solutions on the leaves at the seedling stage and the early flowering stage of the soybeans. Gas chromatography was used to determine the fatty acid content and ratio of the soybean seeds at the maturity stage. The fatty acid ratio of soybean seeds were evaluated by calculating saturated fatty acid(SFA), monounsaturated fatty acid(MUFA), polyunsaturated fatty acid(PUFA), unsaturated fatty acids(UFA), UFA/SFA, index unsaturated fatty acid(IUFA) and double bond index(DBI).(MUFA+PUFA)/SFA was used to study the effects of rare earths on the quality of soybean seeds. The results showed that spraying the rare earth solution could significantly optimize the fatty acid distribution ratio of soybean seeds. Compared with the control group(CK), stearic acid was reduced by 5% and linolenic acid was reduced by 3.72%. Except for La10, the oleic acid content increased significantly by 2.22%. Under the treatment of 30 mg·L~(-1) CeCl_3, UFA, UFA/SFA and DBI were 83.56, 5.08 and 9.47, respectively, which were the highest in each group. Under the treatment of 150 mg·L~(-1) LaCl_3,(MUFA+PUFA)/SFA was 4.89, the closest meal to the recommended ratio. The above results showed that spraying appropriate concentrations of rare earth at the seedling stage and the early flowering stage could optimize the fatty acid ratio of soybean seeds and improve dietary fatty acid ratios.  相似文献   

3.
This study investigated the effects of the degree of unsaturation(unsaturity) of long-chain fatty acids on microbial protein content and the activities of transaminases and dehydrogenase in vitro using goat rumen fluid as the cultural medium.Six types of fatty acids,stearic acid(C18:0,group A,control group),oleic acid(C18:1,n-9,group B),linoleic acid(C18:2,n-6,group C),α-linolenic acid(C18:3,n-3,group D),arachidonic acid(C20:4,n-6,group E),and eicosapentaenoic acid(C20:5,n-3,group F),were tested,and the inclusion ratio of each fatty acid was 3%(w/w) in total of culture substrate.Samples were taken at 0,3,6,9,12,18 and 24 h,respectively,during culture for analyses.Compared with stearic acid,linoleic acid,a-linolenic acid,and arachidonic acid increased the bacterial protein content,while oleic acid and eicosapentaenoic acid had no significant effects;the protozoal protein content was reduced for all the unsaturated fatty acids,and the magnitude of the reduction appeared to be associated with the degree of unsaturity of fatty acids.The total microbial protein content was dominantly accounted by the protozoal protein content(about 4-9 folds of the bacterial protein),and only increased by linoleic acid,but reduced by oleic acid,arachidonic acid and eicosapentaenoic acid.There were no significant effects in the activities of both glutamic oxaloacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) for all the other fatty acids,except for arachidonic acid which enhanced GOT activity and oleic acid which enhanced GPT activity.The total dehydrogenase activity was positively correlated with the degree of unsaturation of fatty acids.In conclusion,the inclusion of 3%of long-chain unsaturated fatty acids increased bacterial protein content,whereas reduced protozoal protein content and enhanced dehydrogenase activity.The fatty acids with more than three double bonds had detrimental effects on the microbial protein content.This work demonstrates for the first time the effect rule of the unsaturation degree of long-chain fatty acids on the rumen microbial protein in vitro.  相似文献   

4.
The feeding trial was conducted to determine the effects of flaxseed on layer＇s performance and egg quality, and the effects of the dietary ratio of ω-6 fatty acid to ω-3 fatty acid on fatty acid deposition in yolk using flaxseed as resource of ω-3 fatty acid and sunflower seed as resource of ω-6 fatty acid. 192 20-wk-old Newroman commercial hens were randomly divided into 4 treatment groups with 6 replicates containing 8 birds. The rations corresponding with the four treatments were formulated with 4% flaxseed ＋ 9% sunflower seed, 8% flaxseed ＋ 18% sunflower seed, 15% flaxseed, and soybean meal, respectively. Sunflower seed was unshelled and flaxseed was whole. Ratios of ω-6 fatty acid to ω-3 fatty acid in 4 dietary treatments were 5.67, 5.21, 1.07, and 11.83, respectively. It can be concluded that long-term feeding 15% flaxseed had no significant （P〉 0.05） influence on egg production, egg weight, eggshell quality, yolk weight, and percentage of yolk weight. Dietary ratio of ω-6 fatty acid to ω-3 fatty acid had impact on the 0-3 fatty acid levels in yolk, nevertheless yolk fatty acid profiles were different from those of dietary fatty acid compositions.  相似文献   

5.
Intramuscular fat （IMF） content is considered to be a key factor that affects the marbling, tenderness, juiciness and lfavor of pork. To investigate the effects of myristic acid （MA） on the differentiation of porcine intramuscular adipocytes, cells were isolated from longissimus dorsi muscle （LDM） and treated with 0, 10, 50 or 100μmol L-1 MA. The results showed that MA signiifcantly promotes the differentiation of intramuscular adipocytes in a dose-dependent manner. MA also led to a parallel increase in the expression of peroxisome proliferator activated receptor-γ（PPARγ） and adipose-related genes, such as glucose transporter 1 （GLUT1）, lipoprotein lipase （LPL）, adipocyte fatty acid binding protein 4 （FABP4/aP2）, fatty acid translocase （FAT）, acetyl-CoA carboxylaseα（ACCα）, adipose triglyceride lipase （ATGL） and fatty acid synthase （FASN）. However, no signiifcant effects of MA were observed on the expression of CAAT enhancer binding protein-α（C/EBPα） or hormone sensitive lipase （HSL）. The expression of pyruvate dehydrogenase kinase 4 （PDK4） was increased by MA during the early stages of differentiation （day 1-3）. In addition, MA also increased the absolute content of C14 （P〈0.001） and saturated fatty acids （SFA） （P〈0.05） to varying degrees, but no effects were observed on other fatty acids. These results suggest that MA might be able to enhance the IMF content of pork and increase the accumulation of myristic and myristoleic acid in muscle, which might have beneifcial implications for human health.  相似文献   

6.
盐胁迫对水稻幼苗类囊体膜脂肪酸组分的影响(英文)   总被引：11，自引：3，他引：8  
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

7.
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

8.
This study investigated the impacts of the degree of unsaturation(unsaturity) of long-chain fatty acids on volatile fatty acid(VFA) profiles of rumen fermentation in vitro. Six types of long-chain fatty acids, including stearic acid(C18:0, control group), oleic acid(C18:1, n-9), linoleic acid(C18:2, n-6), α-linolenic acid(C18:3, n-3), arachidonic acid(C20:4, n-6) and eicosapentaenoic acid(C20:5, n-3), were tested. Rumen fluid from three goats fitted with ruminal fistulae was used as inoculum and the inclusion rate of long-chain fatty acid was at 3%(w/w) of substrate. Samples were taken for VFA analysis at 0, 3, 6, 9, 12, 18 and 24 h of incubation, respectively. The analysis showed that there were significant differences in the total VFA among treatments, sampling time points, and treatment×time point interactions(P0.01). α-Linolenic acid had the highest total VFA(P0.01) among different long-chain fatty acids tested. The molar proportion of acetate in total VFA significantly differed among treatments(P0.01) and sampling time points(P0.01), but not treatment×time point interactions(P0.05). In contrast, the molar proportion of propionate did not differ among treatments during the whole incubation(P0.05). However, for butyrate molar proportions, significant differences were found not only among sampling time points but also among treatments and treatment×time point interactions(P0.01), with eicosapentaenoic acid having the highest value(P0.01). Additionally, no statistically significant differences were found in the acetate to propionate ratios among treatments groups(P0.05), even the treatments stearic acid and α-linolenic acid were numerically higher than the others. The inclusion of 3% long-chain unsaturated fatty acids differing in the degree of unsaturation brought out a significant quadratic regression relation between the total VFA concentration and the double bond number of fatty acid. In conclusion, the α-linolenic acid with 3 double bonds appeared better for improving rumen microbial fermentation and the total VFA concentration.  相似文献   

9.
[Objective] The aim of the study is to understand the changes of fatty acid composition of rice thylakoid membrane under salt stress.[Method] Under salt stress of different concentrations of NaCl,rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes,and gas chromatograph(1890)was used to analyze fatty acid composition.[Result] Fatty acid component 14∶0,18∶0,16∶1(3t),18∶1 in both the two experimental materials showed little variations in the first four days of salt stress,whereafter they increased slightly;while the fatty acid component 16∶0 and level of saturation of fatty acid(LSFA)showed the similar variation trend in the first four days of treatment compared to those of the fatty acid components mentioned above,whereafter they rose in Pokkali and presented an opposite variation trend in Peta;fatty acid component 18∶3 and level of unsaturation of fatty acid(LUFA)reduced all the time under stress condition,and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group,and in Pokkali was smaller than that in Peta under specific conditions.Meanwhile,level of saturation of fatty acid in both experimental materials increased,and the rising amplitude in Peta was smaller than that of Pokkali.[Conclusion] With regard to LUFA,Pokkali is endowed with more salt tolerance than Peta.  相似文献   

10.
This study evaluated the lard enriched with n-3 polyunsaturated fatty acids(PUFAs) on serum lipid composition in rats.The experiment contained two parts:(A) 20 crossed pigs(Landrace×Min, 60±2 kg) were assigned randomly into two dietary groups: a control group and a group fed the diet added 10% linseed based on the control diet.This part lasted 56 days and the results showed that the levels of the total n-3 PUFA in lard were improved approximately 2-fold(p0.05), when pigs were fed 10% flaxseed compared to a control diet of corn.The lard taken from pigs was melted and preserved for rat experiment;(B) 45 Sprague-Dawley rats at day 36 were randomly fed one of three diets for 42 days: the diet without added fat, the diet contained 10% common lard from pigs fed a conventional corn ration and the diet contained 10% lard enriched with n-3 PUFA from pigs fed 10% flaxseed.Rats fed lard significantly increased(p0.05) serum cholesterol levels compared with rats maintained on a diet without lard.However, rats fed the lard enriched with n-3 PUFA significantly decreased the level of low-density lipoprotein cholesterol in serum(p0.05) and better hepatic antioxidant defense capacities, when compared with rats fed common lard(p0.05).The results showed that pigs fed diet with n-3 PUFA produced lard enriched with n-3 PUFA and thus provided positive health benefits to the consumers.  相似文献   

11.
青海大通牦牛肌间脂肪酸组成分析   总被引：7，自引：0，他引：7  
1材料与方法 1．1材料①供试动物。大通牦牛和大通黄牛。二者分布地区及其生态条件见表1。②试剂及药品。氯仿,氢氧化钾,氯化钠,硫酸氢钠,甲醇（以上试剂均为分析纯）,正己烷（色谱纯）,有机相过滤膜（0．5μm）,高纯氮气（99％）等。21种常规脂肪酸甲酯（FAME）标准品为：C6：0、C8：0、C10：0、C12：0、C13：0、C14：0、C15：0、C16：0、C16：1、C17：0、C18：0、C18：1、C18：2、c9t11C18：2、t10c12C18：2、C18：3、C19：0、C20：0、C20：4、C20：5、C22：6（均购自Nu—Chek公司,纯度≥98％）。  相似文献   

12.
青海大通牦牛肌间脂肪酸组成分析   总被引：1，自引：0，他引：1  
[目的]为进一步合理利用大通牦牛资源提供理论依据。[方法]采用气相色谱仪对大通牦牛肌间脂肪酸进行定性定量测定,分析其组成及含量,并与当地大通黄牛进行比较。[结果]大通牦牛肌间脂肪中饱和脂肪酸总含量极显著低于大通黄牛（P〈0.01）,多不饱和脂肪酸总含量极显著高于大通黄牛（P〈0.01）,单不饱和脂肪酸总量与大通黄牛无明显差异（P〉0.05）。大通牦牛PUFA/SFA和n-6/n-3PUFA均在推荐比例之内。[结论]大通牦牛肌间脂肪酸构成优于当地黄牛,营养价值更高,具有广阔的开发空间。  相似文献   

13.
[目的]探讨犊牦牛线粒体氧自由基代谢水平以及能量代谢的生物学机制。[方法]对青海省大通种牛场6月龄1/4野血犊牦牛心肌和骨骼肌线粒体总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)活性以及丙二醛(MDA)、一氧化氮(NO)含量进行了测定。[结果]野血犊牦牛心肌线粒体中T-AOC、GSH-Px、SOD活性和NO含量均高于骨骼肌,但差异均不显著(P0.05)。心肌线粒体中MDA含量显著高于骨骼肌(P0.05)。[结论]该研究从发育学角度为探讨1/4野血牦牛高原低氧适应性提供理论依据。  相似文献   

14.
乳铁蛋白素（Lactoferricin,Lfcin）是从乳铁蛋白（LF）上被胃蛋白酶水解下来的25个氨基酸残基的小肽,位于乳铁蛋白第二外显子,是乳铁蛋白的抗菌活性中心,其抗菌活性是LF的几百倍。Lfcin具有广谱抗菌活性,对革兰氏阳性菌和革兰氏阴性菌均有效,同时具有抗真菌、抗病毒、抑制肿瘤细胞生长、参与免疫调节等多种生物学功能和维持动物肠道菌群正常生态稳定的功能,从而起到提高动物生产性能的作用。  相似文献   

15.
【目的】比较不同年龄阶段安格斯牛与湘西黄牛屠宰性能和肉营养品质的差异,为丰富湘西黄牛和安格斯牛育种数据,开展湘西黄牛品种改良及新品种（系）培育提供科学依据。【方法】选取初始重基本一致的纯种湘西黄牛与安格斯牛种各30头,统一标准饲养条件单栏饲喂,于6、18和30月龄分批屠宰,测定屠宰性能、肉品质及营养价值。【结果】随着年龄的增加,安格斯牛和湘西黄牛的屠宰性能逐渐提高,在30月龄时安格斯牛屠宰率可达49.98%,熟肉率67.56%,眼肌面积4333.49 mm2,剪切力44.13 N;而湘西黄牛屠宰率为48.98%,熟肉率65.94%,眼肌面积4042.78 mm2,剪切力50.18 N。在同一年龄阶段,安格斯牛与湘西黄牛屠宰率、滴水率、失水率及粗蛋白、铜（Cu）、铁（Fe）和锌（Zn）含量差异不显著（P>0.05,下同）,而眼肌面积和剪切力在各年龄阶段均差异显著（P<0.05,下同）。安格斯牛总氨基酸（TAA）含量在18.61%～19.11%,必需氨基酸（EAA）在7.70%～7.95%;湘西黄牛总氨基酸含量在18.28%～19.43%,必需氨基酸在7.27%～7.82%,同一年龄阶段2个牛种间差异不显著;3个年龄阶段的EAA/TAA均在40.00%左右,但同一年龄阶段2个牛种间差异显著。安格斯牛肉中饱和脂肪酸含量在44.51%～49.58%,单不饱和脂肪酸含量在31.93%～37.74%,多不饱和脂肪酸含量在13.03%～23.19%;湘西黄牛肉中饱和脂肪含量在47.22%～49.70%,单不饱和脂肪酸含量在32.41%～39.53%,多不饱和脂肪酸含量在12.89%～19.33%,在同一年龄阶段安格斯牛肉饱和脂肪酸含量显著低于湘西黄牛,而不饱和脂肪酸含量高于湘西黄牛。【结论】湘西黄牛与安格斯牛在屠宰率和肉品质方面基本一致,适合生产制作优质高端牛肉。该结论也为引进安格斯牛来改良湘西黄牛提高本地牛品种的个体大小及屠宰率提供了科学依据。  相似文献   

16.
大通牦牛皮肤发育规律的研究   总被引：1，自引：0，他引：1  
[目的]探讨大通牦牛的皮肤发育规律。[方法]采用组织学方法对4个年龄段（1日龄、30日龄、180日龄和成年牦牛）大通牦牛的皮肤组织结构进行研究。[结果]4个年龄段大通牦牛的皮肤均表现为表皮薄、真皮特厚的特点,且真皮层内毛细血管较少,表皮层内含大量色素细胞;4个年龄段大通牦牛的全皮厚均表现为出生后至30日龄逐渐变厚、至180日龄时又变薄、至成年时又变厚的一种变化趋势。[结论]为深入了解大通牦牛皮肤适应高原恶劣环境的组织结构特点提供了理论依据。  相似文献   

17.
基于mtDNA cyt b基因全长序列,对中国黄牛、牦牛和水牛的遗传多态性和系统发育关系进行了分析。结果表明,中国黄牛、牦牛和水牛cyt b基因序列中碱基A和T的含量均比较高,A T的平均含量分别为56.6%,58.2%和56.0%。黄牛在cyt b基因全长序列具有较丰富的核苷酸多样性(Pi=0.016 84),水牛的核苷酸多样性相对贫乏(Pi=0.003 51),牦牛的核苷酸多样性介于二者之间(Pi=0.012 28)。中国黄牛与牦牛和欧洲野牛之间的亲缘关系相对较近,而与水牛间的亲缘关系则相对较远,黄牛有普通牛和瘤牛2个母系起源;中国牦牛和斑腾牛以及羯牛的亲缘关系相对较近;中国水牛和沼泽型水牛具有较近的亲缘关系,也可能有2个或者2个以上的母系起源。  相似文献   

18.
藏北牦牛肉成分和营养品质的分析研究   总被引：4，自引：0，他引：4  
洛桑  旦增  布多  马红梅  白玛卓嘎 《安徽农业科学》2009,37(29):14198-14199
［目的］分析藏北牦牛肉营养成分,评价牦牛肉品质特点。［方法］ 在西藏藏北地区,随机选取自然放牧的3-5岁、平均体重约300 kg的牦牛10头为试验组,测定屠宰后牦牛肉的肉色、pH值、嫩度、失水率、系水力和熟肉率以及肉的营养成分。［结果］ 藏北牦牛肉色泽深红,肉的营养成分较全面且均衡。牦牛肉的干物质含量为31.64 g/100 g、蛋白质含量为21.43 g/100 g,脂肪含量为3.12 g/100 g,富含铁、钙等矿物质;与内地黄牛相比,干物质高5.94 g/100 g,脂肪含量低0.49 g/100 g,蛋白质含量高1.24 g/100 g。牦牛肉肌肉嫩度剪切值黑牦牛稍高于当地黄牛,牦牛肉比当地黄牛系水力高6.10%、失水率低3.63%、熟肉率高10.06%。［结论］ 藏北牦牛肉是一种高蛋白质、低脂肪、富含矿物质的优质肉类资源,具有良好的加工性能和较高的生产加工效益。  相似文献   

19.
[目的]探讨大通牦牛的皮肤发育规律。[方法]采用组织学方法对4个年龄段（1、30、180日龄和成年牦牛）大通牦牛的皮肤组织结构进行研究。[结果]4个年龄段大通牦牛的皮肤均表现为表皮薄、真皮特厚的特点,且真皮层内毛细血管较少,表皮层内含大量色素细胞;4个年龄段大通牦牛的全皮厚均表现为出生后至30日龄逐渐变厚、至180日龄时又变薄、至成年时又变厚的一种变化趋势。[结论]为深入了解大通牦牛皮肤适应高原恶劣环境的组织结构特点提供了理论依据。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号