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1.
采用C-带技术对二倍体野燕麦根尖细胞染色体进行了带型分析,以研究该野燕麦染色体的C-带特点.结果表明:二倍体野燕麦具有7对染色体,其上共有35条带,其中长臂具有带纹19条,包括13条中间带(I),6条末端带(T);短臂具有8条带纹,包括2条中间带(I),6条末端带(T),另外还有l条随体带(S)以及7条着丝点带(C),其带型公式为2 n=14=2CIT+ +2 CIT+8 CI+ T+2 CI+ T+S.二倍体野燕麦染色体组成为CC,核型为2A,属于较对称核型,进化指数为7. 相似文献
2.
淡黄花百合根尖染色体C-带分析 总被引：3，自引：0，他引：3
利用Giemsa C-带方法对淡黄花百合(Lilium sulphuFeum Baker)进行了研究.结果表明:淡黄花百合(Lilium sulphureum)的染色体数目为2n=2x=24,每条染色体上都显示出特征带,带纹的深浅差异明显,其带 .型公式为:2n=24:2CI+2I+4CI++2CI++8I++2I+T++2IT++2I+N.染色体F有两条强弱不同的中间带和一条次缢痕带.通过Cdemsa C-带方法可以将淡黄花百合(Lilium sulphureum Baker)的每条染色体区分开. 相似文献
3.
岷江百合根尖染色体的C-分带和FISH分析 总被引：5，自引：0，他引：5
利用Giemsa C-分带和荧光原位杂交(FISH)的方法对岷江百合(L.regale)根尖染色体进行了研究.结果表明岷江百合的带型公式为:2n=24=4I 8CL 2I 6I 2I T 2I T ,每条染色体都显示出了可以明显区别的特征带,带纹强弱差异明显.以45S rDNA为探针对岷江百合根尖染色体进行了荧光原位杂交,杂交点数为5对,分别位于A、B、H和K 4对同源染色体的着丝点区域和一对G染色体的长臂上.通过Giemsa C-带和HSH的方法可以将岷江百合的每条染色体区分开来. 相似文献
4.
提莫菲维小麦与葡萄牙野燕麦远缘杂交后代的SRAP分析 总被引：4，自引：0，他引：4
采用SRAP技术分析提莫菲维小麦与葡萄牙野燕麦远缘杂交后代的真实性及其特点。结果表明,22对SRAP引物中,20对引物在双亲间扩增出多态性条带,其多态性比率为73.81%。me4-em1、me3-em5、me4-em3和me3-em3四对引物在提莫菲维小麦与葡萄牙野燕麦杂交F3株系中扩增出双亲特异带,表明该F3株系具有双亲的遗传物质,该F3株系是提莫菲维小麦和葡萄牙野燕麦成功属间杂交的真实杂种后代。在F3株系的扩增结果中部分双亲带型消失,并且提莫菲维小麦消失的带数远少于野燕麦的;同时有非父母标记新带型出现。杂种后代DNA序列的这种变化可能有利于新形成异源多倍体小麦的快速进化、遗传协调和遗传稳定性。 相似文献
5.
6.
西藏小麦及半野生小麦异染色质分化的C-带研究 总被引：2，自引：0，他引：2
以Giemsa C-带技术研究了西藏小麦、西藏半野生小麦和中国春的异染色质分化。它们之间的带型没有很大的差异,但出现了C-带多态性。多态性主要表现在A、B组染色体和分布于染色体臂的中部及端部。以中国春C-带为标准的比较表明,半野生小麦的多态性所属染色体主要为A组的2A、6A和7A,B组的2B、3B、4B和7B,西藏小麦为2A、7A和7B。推测它们同属一个类群,西藏半野生小麦比西藏小麦和中国春原始,并非是来自栽培小麦的杂交后裔。 相似文献
7.
一粒小麦染色体高分辨显带 总被引：1，自引：0，他引：1
AMD 法显带技术对一粒小麦的染色体进行染色体高分辨显带,经过放线菌素 D 预处理、Ohnuki′s 溶液低渗、固定液中捣碎涂片法制片、Wright-Giemsa 染色等显带程序,可以在一粒小麦的所有染色体上成功地显示丰富的高分辨带纹,并对其进行了分析与配对,平均每条染色体上有20多条深带,整个染色体组共显143条带纹。这将对一粒小麦染 相似文献
8.
抗白粉病普通小麦-簇毛麦新种质的遗传学及生化鉴定 总被引：10，自引：0，他引：10
普通小麦-簇毛麦抗白粉病新种质94G22-1和94-G33-1都对白粉病免疫。其体细胞染色体数为42,花粉母细胞减数分裂中期Ⅰ染色体构型为21个二价体。用感病小麦品种对其测交,F_1代全部对白粉病表现免疫,F_1代花粉母细胞减数分裂中期Ⅰ染色体构型为20个二价体加2个单价体。测交F_2抗感比符合3:1的预期分离比例。体细胞快速荧光原位杂交表明94G22-1、94G33-1都带有两条簇毛麦染色体;染色体C-分带证明这两条簇毛麦染色体是一对6V;重双端体分析确认6V代换的普通小麦染色体是6D。在谷草转氨酶(GOT)同工酶电泳图潜的GOT-2活性区,94G22-1、94G33-1显示了3条酶带,其中1条来自簇毛麦,由6V上的基因编码,亚基组成分析亦确证94G22-1、94G33-1是6D(6V)异代换系。 相似文献
9.
百合属绝大多数种是2n=24的二倍体,其核型具有稳定性,一般为3B型,存在少数多倍化现象,但广泛存在B染色体,百合染色体核型的差异正是环境因素和结构变异共同作用的结果。通过百合属C-带带型中单套染色体条带数及特征染色体可以清晰地区分形态学相似的百合属植物,但采用尿素法进行G带带纹的鉴定有更高的分辨率。原位杂交技术已运用于百合属植物的区分和杂种后代的鉴定,而GISH较FISH更适用于杂种百合的鉴定。 相似文献
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11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
13.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
15.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
16.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
17.
Mohammed Riyazaddin Polavarapu B. Kavi Kishor Are Ashok Kumar Belum V. S. Reddy Rajendra S. Munghate Hari C. Sharma 《Plant Breeding》2015,134(4):423-436
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums. 相似文献
18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf
1) and recessive for the other (Rf
2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph
3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf
1(one of two dominant additive genes controlling early season leafing out) indicated that Rf
1and Rf
2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf
1, and 0.15 for Lf
1-Sph
3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph
3.It is suggested that competitive disadvantage of lf
1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph
3. Poor performance of lf
1- (and possibly lf
2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off). 相似文献
19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho... 相似文献
20.
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed. 相似文献