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布鲁氏菌病（布病）活疫苗是世界各国防控布病的重要生物资源,我国目前主要生产和应用的布病活疫苗为S2株和A19株。生物安全是布病活疫苗生产过程中的必须优先考虑的重要环节,随着兽药GMP（2020年版）的即将实施,规范布病活疫苗的生产与检验,防控生产环节中的生物安全风险,成为迫切需要研究和解决的突出问题。本文通过对布病活疫苗生产检验、设施维修维护、生产人员个人防护等环节的生物安全风险点进行分析,对布病活疫苗生产线提出改进建议,为相关企业动物布病活疫苗生产提供参考。 相似文献
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《中国兽医杂志》2014,(11)
为建立鸡马立克病(MD)活疫苗中污染禽网状内皮组织增生症病毒(REV)的间接免疫荧光检测方法,本研究通过向MD活疫苗中添加不同剂量的REV,用拟定的方法对样品检测REV。结果表明,Ⅰ型MD活疫苗、火鸡疱疹病毒活疫苗(Fc-126株)和MD二价活疫苗(HVT+CVI988株)中污染REV的最低检出量分别为每500羽份疫苗中污染5TCID50、10TCID50和15TCID50的REV。应用建立的方法对国内10家企业生产的43批MD活疫苗进行了检验,结果显示,2个企业生产的4批疫苗REV检测阳性。随机选取了5批IFA检测阴性样品和4批IFA检测阳性样品,按鸡检查法进行外源病毒检验,结果两种方法对REV污染检验结果的符合率为100%。因此,本研究建立的IFA法可替代鸡检查法,用于疫苗中REV污染的检验。 相似文献
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近日,内蒙古金宇集团生物药品厂冻干弱病毒活疫苗生产车间、产品检验楼及检验动物房通过了农业部兽药GMP验收专家组的现场验收。涉及检查项目共199项经专家组综合评定合格率为94.32%,从而成为亚洲最大的冻干弱病毒活疫苗生产车间,该车间可年产上百亿冻干弱病毒活疫苗。冻干弱病毒活疫苗生产车间的投产,对及时有效地控制畜禽疫病的暴发,保护畜禽健康和保障动物性食品安全将发挥重要作用。内蒙古建冻干活疫苗车间@古明 相似文献
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按中国兽药典-生产、检验用动物标准中的实验动物普通级标准的要求,猪瘟活疫苗原料兔中不得检出体外寄生虫,主要指跳蚤和兔疥螨。尤以跳蚤对以人工密集型的猪瘟活疫苗（兔源）生产和人身健康的影响最大。 相似文献
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对二〇〇五年版《中华人民共和国兽药典》中鸭瘟活疫苗外源病毒检验的鸡检查法进行了修订,确定选用9-12周龄SPF鸡,在原药典规定方法的基础上增加低剂量基础免疫程序,并用该外源病毒检验方法对5批鸭瘟活疫苗进行了验证,结果表明修订后的鸭瘟活疫苗外源病毒检验方法可靠,可操作性强。 相似文献
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新型疫苗研究概况 总被引：1，自引：1，他引：0
新型疫苗研究对于畜禽传染病的防控具有重要意义.传统疫苗是基于抗原刺激机体产生特异性抗体的原理,但大多数激发机体产生体液免疫,很难启动细胞免疫.鉴于传统疫苗所存在的缺点,开发新型疫苗成为必然趋势.随着免疫学和分子生物学技术的快速发展,近年来出现了各种不同的新型疫苗,这类疫苗的出现弥补了传统疫苗的缺点,也填补了疫苗种类的空白.文章中重点论述了重组活载体疫苗、树突状细胞疫苗、T细胞疫苗、食物疫苗、DNA疫苗、RNA复制子疫苗、多肽疫苗和抗独特型抗体疫苗等8种新型疫苗的作用机制和研究概况,并对各新型疫苗的优缺点、存在的问题及应用前景等进行了论述. 相似文献
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国内外常用新城疫活疫苗的毒力和血凝滴度测定试验报告 总被引：1，自引：0，他引：1
本试验通过对国内外常用新城疫弱毒疫苗最小致死量（MLD）测定、鸡胚平均致死时间（MDT）测定，对疫苗的毒力强弱和致病型进行分析。对疫苗的血凝试验（HA）滴度进行测定。根据疫苗的抗原含量推断免疫效力。测定新城疫不同毒株的弱毒疫苗单苗和与传染性支气管火H120等的二联苗或三联苗共14种，对其中9种疫苗进行了鸡胚最小致死量（MLD）测定和鸡胚平均致死时间（MDT）试验，对所有的疫苗都进行了HA试验。不管是进口疫苗还是国产疫苗其MDT有较大的差异，其毒力有强有弱，有的为中发型（中等毒力）疫苗，主要是温和型（低毒力）疫苗，最小致死量（MLD）范围为10^-8-10^-10/0.1ml。不同厂家生产的疫苗HA滴度有较大的区别。国产疫苗的质量与进口疫苗无明显的差异，有的比进口疫苗质量还好。对出口养禽企业选用低毒力、HA滴度高的疫苗具有较好的指导作用。 相似文献
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H D Stone 《Avian diseases》1991,35(1):8-16
Experimental Newcastle disease oil-emulsion vaccines were prepared by manually shaking small quantities of antigen, surfactant, and mineral oil and compared with oil-emulsion vaccines made by the more conventional mechanical methods. Emulsion stability (prolonged emulsification of aqueous antigen) of the manually prepared vaccines was achieved by four different processes and tested for efficacy in broilers. White rock broilers were vaccinated at 3-5 weeks of age and bled at 1- to 2 week intervals thereafter for 8 weeks. Cumulative 8-week hemagglutination-inhibition (HI) mean titers (reciprocals) ranged from 15 to 250 for manually emulsified vaccines and from 18 to 240 for mechanically emulsified vaccines. Eight-week cumulative mean HI responses induced by manually emulsified vaccines were never significantly (P less than 0.05) lower than their specific mechanically emulsified comparison vaccines and were occasionally significantly higher. Highest HI titers were induced when hydrophile-lipophile balances of 7, 9, and 6 were used for the preparation of manually emulsified vaccines. In general, most of the vaccine emulsions were stable for more than 30 days. Vaccine efficacy, however, was not always diminished by poor emulsion stability. These results indicate that manual emulsification can be used for the production of oil-emulsion vaccines of high efficacy that can provide benefits over existing mechanical methods. 相似文献
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M M Rweyemamu O Umehara D de Lucca-Neto M C Baltazar F E Vicente R da R Medeiros-Neto 《American journal of veterinary research》1986,47(6):1243-1248
Avridine, a lipoidal amine with interferon-inducing and adjuvant properties, was an effective adjuvant for Newcastle disease antigen (NDA) in chickens. Eleven vaccine lots were evaluated: 2 commercial water-in-oil vaccines, 4 experimental oil emulsion vaccines, 4 avridine-containing vaccines, and a control lot of nonadjuvanted antigen. Avridine significantly enhanced the immunologic responses of chickens against NDA. Chickens vaccinated with the avridine-containing vaccines had significantly higher antibody titers (hemagglutination inhibition) than did chickens vaccinated with the commercial vaccines. Experimental oil emulsion vaccines prepared from the same antigens as avridine-adjuvanted vaccines induced higher hemagglutination inhibition antibody titers after primary but not after booster vaccination. Use of avridine as an adjuvant for NDA in vaccines for chickens induced immunologic protection rates similar to those induced by oil emulsion vaccines, without causing the reactogenic and tissue residue problems associated with the use of oil vaccines in chickens. 相似文献
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口蹄疫双佐剂灭活疫苗的研究 总被引：2，自引：1，他引：1
在口蹄疫二价灭活疫苗（O型、AsiaⅠ型）常规使用法国SEPPIC公司206佐剂的基础上,设计了一种缓释作用更强的双佐剂成分疫苗。通过MTT法检测淋巴细胞增殖能力、液相阻断ELISA法检测口蹄疫抗体效价及攻毒试验测定PD50来评判该双佐剂疫苗与常规疫苗的效果差异。结果显示,双佐剂疫苗组细胞免疫水平（淋巴细胞刺激指数SI为1.235±0.060）比常规佐剂疫苗组（SI为1.115±0.035）和对照组（SI为1.010±0.045）高,与常规疫苗组相比差异显著（P＜0.05）,与空白对照组相比,差异极显著（P＜0.01）。双佐剂疫苗组O型和AsiaⅠ型抗体与常规佐剂疫苗组相比,全剂量组抗原量较充分,两组抗体水平差距不大;1/3剂量组和1/9剂量组由于抗原量较少和强缓释作用,导致抗体水平明显较低。攻毒保护结果为双佐剂疫苗组略高于常规佐剂疫苗组,前者每头份疫苗AsiaⅠ型为9.0 PD50,O型为11.84 PD50,后者每头份疫苗AsiaⅠ型为9.0 PD50,O型为9.0 PD50。由分析结果可见,双佐剂疫苗可引起较好的细胞免疫应答和缓释作用,达到好的攻毒保护效果。 相似文献
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The immunogenicity of DNA vaccines encoding three different Taenia ovis host-protective antigens was compared in mice and sheep. DNA vaccines encoding the 45W, 18k and 16k antigens of T. ovis were constructed. The ability of DNA vaccines encoding the 45W and 18k genes to express antigen was confirmed by Western blotting of transfected Cos-7 cells. BALB/c mice were vaccinated intramuscularly with 45W, 18k or 16k DNA vaccines and the humoral immune response analysed by ELISA. DNA vaccines expressing 45W, 18k or 16k antigen were immunogenic in mice and generated significant titres of antigen-specific antibody. Intramuscular vaccination of outbred sheep with the T. ovis DNA vaccines generated significantly lower titres of 45W-specific antibody and failed to generate 18k or 16k-specific antibody. The findings of this study show that each of the three T. ovis host-protective antigens are amenable to delivery via DNA vaccines, and that the parameters governing the efficacy of DNA vaccines in sheep require further investigation. 相似文献
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抗菌药对马立克氏病活疫苗毒性的作用 总被引：1，自引：0，他引：1
用不同剂量的青霉素钾、硫酸链霉素、硫酸卡那霉素、硫酸庆大霉素和恩诺沙星等５种常见抗菌药加入ＨＶＴ冻干菌、ＣＶＩ９８８细胞结合苗和Ｚ４＋ＦＣ１２６双价活疫苗中,作用１ｈ后,分别取样计数,研究其对马立克氏病（ＭＤ）疫苗的毒性作用。结果表明：５种抗菌药加入ＭＤ疫苗稀释液后,ｐＨ值变化较大,ＭＤ疫苗空斑数显著减少,以至使ＭＤ疫苗失效。 相似文献