共查询到18条相似文献，搜索用时 93 毫秒
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镉中毒对鸡体内GSH-Px、SOD的影响及硒颉颃效应的研究 总被引：2，自引：0，他引：2
选用90只100日龄依沙褐公鸡,随机平均分为3组,对照组饲喂基础日粮,加镉组饲喂基础日粮 CdCl2150 mg/kg,加硒镉组饲喂基础日粮 CdCl2150 mg/kg Na2SeO322 mg/kg,通过在日粮中添加一定剂量的镉或硒与镉,建立亚慢性镉中毒及硒颉颃模型;亚慢性镉中毒可致鸡体内血清和肝脏谷胱甘肽过氧化物酶、超氧化物歧化酶活性降低,构成了镉损伤肝脏的病理学基础。加硒后可有效地提高机体的抗氧化能力。 相似文献
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镉对鸡抗氧化系统功能的影响及硒颉颃的效应 总被引：6，自引：0，他引：6
30只100日龄海兰公鸡随机分为对照组、加镉组、加硒镉组,对照组饲喂基础日粮,加镉组饲喂基础日粮 CdCl2150 mg/kg,加硒镉组饲喂基础日粮 CdCl2150 mg/kg Se 10 mg/kg,分笼常规饲养,试验期为60 d,复制出鸡亚慢性镉中毒及硒颉颃模型.试验结果显示,睾丸脏器系数为对照组、加硒镉组至加镉组依次降低;血清和睾丸中谷胱甘肽过氧化物酶(GSH-Px)活性为对照组、加硒镉组至加镉组依次降低,变化比较明显;血清和睾丸中超氧化物歧化酶(SOD)活性为对照组、加硒镉组至加镉组依次降低,变化比较明显;血清和睾丸中丙二醛(MDA)含量为对照组、加硒镉组至加镉组依次升高.揭示了镉对鸡抗氧化系统功能的影响,构成了镉损伤机体的病理学基础.补硒后可降低镉的损伤. 相似文献
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氯化镉对小鼠的病理损伤和硒的保护效应 总被引：2，自引：0，他引：2
采用腹腔注射的方法在９０只昆明种小鼠中进行了镉中毒与硒解毒的研究。结果表明,镉中毒组在注射后１～５周内,表现出明显的病理变化,特征为全身小动脉血管及肝、肾、心、肺、脑等器官有严重的病理损伤,且肝脏超微病变明显;而硒解毒组小鼠上述变化明显减轻,以肝、心、脾表现最为明显,结果表明硒可颉颃镉在小鼠的毒性。 相似文献
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氧化应激在亚慢性镉中毒引起的肝肾细胞凋亡中的作用 总被引：2，自引：0，他引：2
为了探明镉中毒时氧化应激与细胞凋亡的关系，以公鸡为试验动物，在日粮中添加CdCl2 150mg／kg，肝脏、肾脏和血清中谷胱甘肽过氧化物酶（GSH～Px）活性、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量以及肝、肾细胞凋亡的检测显示镉使鸡血清、肝脏和肾脏中GSH～Px和SOD活性降低，MDA含量增多，并诱导肝肾细胞凋亡的发生。结果表明。镉能够诱发鸡肝肾细胞发生氧化胁迫，并诱导细胞凋亡的发生。提示氧化应激与细胞凋亡存在一定的关系。 相似文献
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为研究亚硒酸钠对游离脂肪酸 (free fatty acid,FFA) 致体外培养大鼠肝细胞凋亡和线粒体膜电位降低的保护作用,本试验通过胶原酶两步灌流法分离培养肝细胞,在细胞培养液中添加FFA和亚硒酸钠 (0.1 μmol/L)测定细胞的凋亡情况、细胞功能和线粒体膜电位。结果表明,随着培养液中FFA浓度的升高,细胞凋亡加重,肝细胞功能受到影响,线粒体膜电位降低且FFA的这种作用呈剂量依赖性;亚硒酸钠能抑制由FFA引起的肝细胞的上述变化。 相似文献
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Levels of trace elements in liver from Norwegian moose, reindeer and red deer in relation to atmospheric deposition 总被引：1，自引：0，他引：1
The levels of antimony, arsenic, cadmium, copper, lead, mercury, molybdenum, selenium and zinc were determined in liver of moose, reindeer and red deer from differen localities in Norway. Reindeer had considerably higher levels of mercury, lead and arsenic than moose and red deer. These findings are considered to be related to differences in feeding habits since reindeer on highland plateaus feed mainly on lichens which have been shown to accumulate trace elements. The levels of arsenic, and to some extent also selenium and cadmium, in moose and reindeer liver showed a north-south gradient closely related to the regional distribution of these elements in the forest moss. As regards lead, a north-south gradient and a relationship to moss levels, were found in moose liver only. It therefore seems that atmospheric deposition of the elements mercury, lead, arsenic, cadmium and selenium contribute to the body burden of these elements in cervides in Norway. There was no evidence of these elements accumulating to toxic levels. 相似文献
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为探讨铅和（或）镉的毒性机制及硒的拮抗作用,在大鼠肾细胞培养过程中添加不同浓度的铅、镉和硒,在显微镜下观察细胞形态,测定细胞凋亡率和细胞周期及上清液脂质过氧化指标。结果表明：（1）20μmol/L以上铅、镉单独染毒组细胞损伤严重,加硒后细胞的形态损伤有降低的趋势;（2）铅、镉单独和联合染毒组GSH-Px、SOD活性随染毒剂量的增加,呈逐渐下降趋势（P〈0.05）,而MDA含量和细胞凋亡率则呈升高趋势（P〈0.05）,具有明显的剂量-效应关系和剂量-时间关系;（3）在铅、镉单独和联合染毒组加硒,脂质过氧化指标呈下降趋势（P〈0.05）,说明硒对铅、镉及其联合暴露所致的脂质过氧化损伤有一定的保护作用（P〈0.05）。 相似文献
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The content of cadmium and selenium in horse kidneys from Jutland , Denmark, in relation to age, local geographical variation and possible relationship between the two elements has been investigated. During the winter of 1982-1983 kidneys from 50 horses were sampled and analysed for cadmium and selenium. The cadmium content of the horse kidneys was recorded in connection with the age of the horses. The cadmium level increases until the animal has reached approximately 7 years of age. At this age the cadmium concentration levels off. A significant regional difference was shown. The cadmium content is higher in horse kidneys from South and Central Jutland than in kidneys from North Jutland (Fig. 2). This geographic pattern is consistent with the one for atmospheric deposition of cadmium and cadmium content in Danish cattle kidneys from Jutland . The cadmium level in kidneys from Danish horses is considerably lower than the level in Norwegian and Swedish horse kidneys. Differences in water hardness and atmospheric deposition may explain some of this difference within Scandinavian countries. The selenium concentration shows no relationship with neither age, cadmium content nor place of rearing. So, the study did not reveal any relationship between the cadmium and selenium concentrations in horse kidneys. The high level of cadmium found in Danish horse kidneys emphasizes the importance of a prohibition against the use of the kidneys in animal and human nutrition, as proposed by others. 相似文献
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旨在研究铁死亡在镉致鸡肝组织损伤中的作用。选取30只1日龄海兰白蛋公鸡，预饲7 d后，随机平均分为对照组(C组)和镉暴露试验组(Cd组),C组鸡饲喂基础日粮，Cd组鸡饲喂混有140 mg·kg-1 CdCl2的基础日粮。镉暴露后20、40和60 d取样，观察肝组织病理及超微病理学变化，检测肝功能酶活性，肝组织氧化应激水平，肝中炎症因子、铁死亡相关因子及线粒体融合相关因子表达水平；建立肝癌细胞系(LMH)染镉细胞模型，同时设立铁死亡激活剂和铁死亡抑制剂染镉细胞组，镉暴露24 h,检测细胞活性、炎症因子表达、铁死亡相关因子和线粒体融合相关因子表达。结果发现，Cd组鸡血清中肝功能酶活性明显升高；肝细胞排列紊乱、空泡变性甚至坏死，肝组织淤血，浆液-纤维素性渗出，炎性细胞浸润；肝细胞线粒体体积缩小、嵴断裂。肝中炎症因子LOX、NF-κB、TNF-α、IL-6和IL-1β mRNA相对表达上调，TNF-α、IL-6和IL-1β含量显著上升；过氧化物MDA含量明显升高、抗氧化酶GSH-Px与SOD活性显著降低；铁死亡代谢相关因子铁蛋白重链1(FTH1)和转... 相似文献
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An examination was made of the relationship in Romney sheep between selenium deficiency and (i) the state of the liver drug metabolising enzyme system, (ii) subclinical liver damage, (iii) susceptibility to facial eczema, and (iv) susceptibility to ryegrasss staggers. The microsomal electron transport detoxifying enzymes based on cytochrome P-450 were unaffected by Se deficiency, suggesting that these enzymes are uninduced on ryegrass/clover pastures. Selenium deficiency had no effect on sporidesmin-induced liver damage, which makes it unlikely that peroxidative events countered by Se-dependent glutathione peroxidase (E.C.1.11.10) have a major role in the mechanism of sporidesmin toxicity. Selenium deficiency did appear to suppress the development of photosensitisation. There was no major effect on ryegrass staggers and no deficiency-induced subclinical liver damage. 相似文献
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硒和碘营养对蛋鸡不同组织抗氧化酶活性的影响 总被引：2，自引：1，他引：2
本试验旨在探讨饲粮中添加不同剂量的硒和碘对蛋鸡心脏、肝脏、脾脏和甲状腺中几种抗氧化酶活性的影响。288只33周龄的新罗曼蛋鸡,饲喂添加不同剂量硒和碘(0,0;0.2,0:0,0.2:0.2,0.2mgkg)的日粮12周,试验结果如下:硒缺乏时,心脏和肝脏中的GSHPx活性下降(P=0.073);碘缺乏时,心脏和肝脏中的CAT活性下降(P=0.055,P=0.097),肝脏中的GSHPx活性降低(P=0.093);硒碘互作对心脏(P<0.01)和肝脏(P<0.05)中的TSOD和CAT活性影响显著,对脾脏中的TSOD(CAT+GSHPx)值影响极显著(P<0.01)。结果表明:碘能够影响肝脏中含硒酶的活性,硒碘的互作能够影响心脏、肝脏和脾脏的抗氧化酶活性。 相似文献
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Nadine Pa?lack Barbara Mainzer Monika Lahrssen-Wiederholt Helmut Schafft Richard Palavinskas Angele Breithaupt Jürgen Zentek 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):57-66
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations. 相似文献
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The medical records of eight horses with histological evidence of myodegeneration of the masseter muscles were examined. While they were alive their most common clinical signs had included difficulty in eating or opening their mouths, weight loss, difficulty in moving, and noticeable atrophy of the masseter muscles. The serum activities of muscle enzymes were abnormally high in all of the horses. Whole blood and/or liver selenium and vitamin E concentrations were less than the reference ranges in some of the horses. The lesions varied with the stage of the disease and consisted of swelling and discoloration, or muscle atrophy and fibrosis. Histologically, the muscle changes ranged from acute to subacute degeneration, with regenerative changes accompanying ongoing degeneration, to chronic degeneration with fibrotic replacement of muscle tissue. There were changes in the masseter muscle of all the horses, but some had widespread lesions in skeletal muscle, and a few also had myocardial lesions. 相似文献