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1.
该试验采用过碘酸钠氧化法研制成功了抗鸡传染性法氏囊病病毒酶标抗体。通过进行双抗体夹心法ＥＬＩＳＡ试验，对不同送检病料中的传染性法氏囊病病毒进行了检测。结果表明：用过碘酸钠氧化法制备酶标抗体方法简便、省时；用双抗体夹心法ＥＬＩＳＡ试验检测传染性法氏囊病病毒，具有灵敏度高、特异性强等优点。  相似文献   

2.
间接酶联免疫吸附试验检测禽流感抗体的最佳工作条件   总被引：2，自引：0，他引：2  
禽流感病毒（ＡＩＶ）感染的鸡胚尿囊液经差速离心后,再经蔗糖密度梯度离心,提纯ＡＩＶ,纯化的ＡＩＶ经ＮＰ４０处理并反复冻融,即为ＡＩＥＬＩＳＡ抗原,用该抗原包被聚苯乙烯微量反应板。将健康鸡ＩｇＧ提纯后免疫兔,制备兔抗鸡ＩｇＧ,用过碘酸钠法制备辣根过氧化物酶标记的兔抗鸡ＩｇＧ。确立了间接酶联免疫吸附试验（ＥＬＩＳＡ）检测禽流感抗体的最适工作条件,即：抗原包被浓度１．９～３．８μｇ／ｍｌ,每孔１００μｌ,４℃冰箱过夜;用含０．５％牛血清白蛋白（ＢＳＡ）的磷酸盐缓冲液,３７℃封闭６０分钟;待检血清最佳稀释度为１８０～１６４０,作用６０分钟;酶标抗体作１２０００稀释,作用６０分钟。根据对５２份ＳＰＦ鸡血清的检测结果制定了判定标准。  相似文献   

3.
现代诊断技术在兽医学上的应用及其进展   总被引：2，自引：0，他引：2  
1　免疫酶技术 (ＥＩＡ)ＥＩＡ是根据抗原 -抗体结合的特异性 ,以酶作标记物 ,酶对底物具高效催化作用的原理而设计 ,既可用于抗原的检查 ,也常用于血清抗体的检测。1 .1　酶联免疫吸附试验 (ＥＬＩＳＡ)ＥＬＩＳＡ是应用最广泛的免疫酶技术 ,包括间接法、夹心法、竞争法等。自ＶＯＬＬＥＲ和ＢＩＤＷＥＬＬ(1 975 )首先报道了应用间接ＥＬＩＳＡ方法检测病毒特异性抗体以来 ,ＥＬＩＳＡ方法已普遍应用于传染病的流行病学普查、抗体水平的评价以及疫苗质量的监控和标化等 ,迄今为止大多数动物病原体均已发展或报道了ＥＬＩＳＡ方法的研究与…  相似文献   

4.
ELISA双抗体夹心法诊断犬冠状病毒肠炎   总被引：1，自引：0，他引：1  
从冠状病毒（ＣＣＶ）阳性病犬粪便提取物中提纯抗原，制备豚鼠抗血清，用饱和硫酸铵盐析法粗提，再经ＱＡＥ－ＳｅｐｈａｄｅｘＡ５０离子柱层析，提取ＩｇＧ，以辣根过氧化物酶（ＨＲＰ）标记，制备酶标抗体，按ＥＬＩＳＡ双抗体夹心法操作术式，建立检测犬冠状病毒的ＥＬＩＳＡ双抗体夹心法，诊断犬冠状病毒肠炎粪样，并以电镜检查比较，证明该法特异、敏感，可在４小时内报告结果。  相似文献   

5.
酶联免疫吸附测定法在饲料毒素检测中的应用   总被引：8，自引：0，他引：8  
1　酶联免疫吸附测定法 (ＥＬＩＳＡ)的基本原理及特点ＥＬＩＳＡ法是把抗原抗体的免疫反应和酶的高效催化作用原理有机地结合起来的一种检测技术。该技术的主要依据有 3点 :1 )抗体吸附在固相载体表面后 ,仍具有和抗原产生免疫反应的特性 ;2 )抗原和酶结合所形成的结合物 (酶标抗原 ) ,仍具有免疫反应特性和酶的催化特性 ;3)酶标抗原和抗体结合后 ,结合物中的酶 ,能催化底物生成有色物质 ,且颜色的深浅 ,可作为判定抗原含量的依据。ＥＬＩＳＡ测定法主要分 3类 :间接法、夹心法和竞争法。饲料中毒素大多数是小分子半抗原物质 ,因此主要采用…  相似文献   

6.
在内蒙古牙克石市图里河镇非马传贫注苗区，用琼脂扩散试验（ＩＤ）和酶联免疫吸附试验（ＥＬＩＳＡ）两种方法对６４匹马进行了对比检查，检出ＩＤ、ＥＬＩＳＡ双阳性４匹、ＤＬＩＳＡ单阳性马３匹。对３匹ＩＤ阴性，ＥＬＩＳＡ阳性马血清用不同浓度琼扩抗原重复检测结果为阴性，而用不同批次的ＥＬＩＳＡ抗原包被板及酶标抗体检测结果均为陧阳性，用马传盆病毒抗原对３匹单阳性马血清中和后，再进行ＥＬＩＳＡ测定，ＯＤ值均降低５  相似文献   

7.
Dot—ELISA检测猪生殖和呼吸综合征抗体的研究   总被引：7，自引：3，他引：7  
用差速离心法提纯ＰＲＲＳ病毒，利用ＮＣ膜作为载体，在国内外首次成功建立了检测ＰＲＲＳ血清抗体的斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）。抗原包被浓度为１００μｇ／ｍｌ，被检血清工作浓度为１：１０，酶标兔抗猪ＩｇＧ工作浓度为１：６００。对７２份北京地区猪血清分别用Ｄｏｔ－ＥＬＩＳＡ和ＩＦ检测，Ｄｏｔ－ＥＬＩＳＡ检测阳性率为３３．３％，ＩＦ检测阳履率为３０．６％，与ＩＦ符合率较高，对部分待检血清检测  相似文献   

8.
杨永钦 《畜牧与兽医》1995,27(4):181-182
ＥＬＩＳＡ的放大系统杨永钦（云南省畜牧兽医研究所，６５０２２４）ＥＬＩＳＡ广泛用于抗原抗体的分析检测，八十年代以来，研究者从多方面探索了提高ＥＬＩＳＡ敏感性方法，包括选择理想的标记酶［１］，用荧光法检测标记酶的活性［２］，使用高亲和力的抗体及引进放大...  相似文献   

9.
间接酶联免疫吸附试验检测禽流感抗体的最佳工作条件   总被引：26，自引：0，他引：26  
禽流感病毒（ＡＩＶ）感染的鸡胚囊液经差速离心后,再经蔗糖密度梯度离心,提纯ＡＩＶ,纯化的ＡＩＶ经ＮＰ４０处理并反复冻融,即为ＡＩＥＬＩＳＡ抗原,用该抗原包被聚苯乙烯微量反应板,将健康鸡ＩｇＧ提纯后免疫兔,制备兔抗鸡ＩｇＧ用过碘酸钠法制备辣根过氧化物酶标记的兔抗鸡ＩｇＧ,确立了间接酶联免疫吸附试验（ＥＬＩＳＡ）检测禽流感抗最适工作条件,即：抗原包被浓度１．９～３．８μｇ／ｍｌ,每孔１００μｌ４℃冰箱  相似文献   

10.
辣根过氧化物酶(HRP)3种方法标记兔抗牛IgG效果的比较   总被引：3，自引：0，他引：3  
酶联免疫吸附试验（ＥＬＩＳＡ）已广泛应用于各种疾病的诊断，作为一种理想的酶标记物，应具备标记率高、制作简便、保持活性、稳定显色。这和酶及ＩｇＧ的含量以及酶标记物的稀释度有密切关系。因此，我们在酶标记物制备中，对过碘酸钠氧化法，简易戊二醛二步法和戊二醛过碘酸钠法３种方法所制作的酶标记物的含量和浓度作了测定和比较，现将结果报告如下。１　材料与方法１．１　试验材料　辣根过氧化物酶：采用国产上海东风生化试剂厂ＨＲＰ（ＲＺ＜３．０）；抗原：人工感染犬后从血中获得可溶性锥虫抗原；抗体：兔抗牛ＩｇＧ；１０头…  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
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17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

20.
Tropical Animal Health and Production -  相似文献   

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