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1.
将小白鼠生殖系统影响实验筛选的3个中药方剂分别应用于10只处于间情期的健康京巴母犬。每只母犬每天经口灌服复方中草药煎剂4 mL,连用20 d,每天观察试验犬的行为变化、外观变化,在用药后的第14、18、20、25天捕杀试验犬,取其卵巢、子宫和阴道,制成切片,于光学显微镜下观察卵巢、子宫内膜和阴道等的组织学变化,中药方C应用的健康京巴母犬,在用药期间定期静脉采血,分离血清,测定E2、P4和IL-1β、IL-6的浓度变化。结果表明：中药组方C诱导犬发情时阴道黏膜增厚、充血、上皮脱落,子宫内膜和子宫腺体增生明显,卵巢出现了成熟卵泡和排卵点,E2在应用15 d达到峰值,峰值过后,E2浓度迅速下降;P4浓度在用药时及整个发情前期均很低,到17 d浓度开始持续升高;IL-1β浓度在17 d达到峰值,以后开始下降;IL-6浓度在5 d开始持续升高,到21 d排卵前后达到峰值。说明中药诱导非繁殖季节母犬发情是可行的。 相似文献
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为筛选具有显著治疗犬蠕形螨的中药制剂,试验将中药小茴香、蛇床子、羊蹄根采用水煮醇沉及蒸馏等提取方法制备成中药制剂犬螨灵,并观察其对犬蠕形螨的疗效。将犬螨灵设为高、中、低3个浓度,其生药含量分别为2.0、1.0和0.5 g/mL,采用点滴法进行体外抑杀蠕形螨试验,在此基础上对12只接种蠕形螨的试验犬进行治疗试验,为验证治疗效果,对临床上已确诊的45只患有蠕形螨的病犬分3组进行临床治疗试验。结果显示,中药犬螨灵3种剂量均有较强的体外抑杀螨虫作用,高剂量组作用后4 h即可完全抑杀蠕形螨,1%伊维菌素对照组则为作用后8 h;高剂量组在治疗3周后,患处有大量新毛生长,红疹结节和皮屑消失,患处皮肤已痊愈,1%伊维菌素治疗组患犬皮肤好转,仅有少量新毛生长,不能达到痊愈标准;高、中剂量组皮肤刮取物螨虫数量平均比例及停药1个月后复发率均为0,1%伊维菌素治疗组皮肤刮取物螨虫数量平均比例及停药1月后的复发率分别为10.63%和9.17%。高剂量中药犬螨灵与1%伊维菌素两种药物联合治疗组,对临床上患病犬进行3周治疗,其虫卵平均转阴率、治愈率与平均治愈天数分别为100.00%、93.33%和19.56 d,其效果均优于中药犬螨灵与1%伊维菌素单独治疗组。结果表明,高剂量自制中药制剂犬螨灵对犬蠕形螨体外抑杀作用、接种蠕形螨试验犬与临床自然感染病例均有显著的治疗效果,优于1%伊维菌素对照组,犬螨灵作为中药杀螨虫制剂具有较好的市场开发前景。 相似文献
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In order to screen the Chinese herbal medicine for the treatment of Demodex canis, canine acariasis spirit was prepared by Foeniculum vulgare, Fructus cnidii and Bauhinia by water alcohol precipitation and distillation extraction methods. To observe the effect on Demodex canis, canine acariasis spirit was set to high, medium and low concentrations, the content of crude drug were 2.0, 1.0 and 0.5 g/mL, respectively. The experiment of killing Demodex canis was carried out by drop method. On this basis, 12 dogs inoculated with Demodex canis were treated. In order to verify the therapeutic effect, 45 clinically diagnosed dogs with Demodex canis were divided into three groups. The results showed that 3 doses of the traditional Chinese medicine canine acariasis spirit all had strong inhibition effect to kill mites, the high dose group could completely kill Demodex canis only 4 h after treatment, the action of 1% ivermectin control group was 8 h after treatment; 3 weeks later, a large number of new hair grew, rash and skin nodules disappeared, the skin lesion had healed in the affected area of high dose group, in 1% ivermectin treatment group, the dog skin improved, only a small amount of new hair grew, it couldn't cure standard; In the high and middle dose group, the average number of the ratio of skin scraping mites and the recurrence rate after drug withdrawal was 0 in one month later; The average number of mites proportion and recurrence rates of skin scrapings in 1% ivermectin treatment group were 10.63% and 9.17% in one month later; After 3 weeks of treatment, the total average eggs negative rate, cure rate and average cure days were 100.00%, 93.33% and 19.56 d in high dose traditional Chinese medicine canine acariasis spirit and 1% ivermectin combined treatment group, the results were better than the traditional Chinese medicine canine acariasis spirit and 1% ivermectin alone treatment group. The results showed that the effect of the high dose group self-made Chinese medicine canine acariasis spirit on killing Demodex canis in vitro, inoculation of Demodex canis and clinical therapeutic effect of natural infection cases were significantly better than 1% ivermectin control group, canine acariasis spirit as a traditional Chinese medicine preparation would have a good market development prospects. 相似文献
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试验旨在研究中草药、益生素及其合剂对肉仔鸡生产性能的影响。选择3500只1日龄罗斯308肉仔鸡随机分为7组,每组设5个重复,每个重复100只鸡,对照组饲喂基础日粮,试验1、2、3、4、5组分别在基础日粮的基础上添加0.5%的药方1、药方2、益生素、中草药益生素合剂药方3、中草药益生素合剂药方4组;抗生素组为基础日粮中添加500 mg/kg杆菌肽锌。结果表明,中草药益生素合剂药方3组的体重、料重比、死淘率与对照组相比差异显著(P＜0.05),与抗生素组相比差异不显著(P＞0.05),优于其它处理组。因此,药方3促生长抗病效果较好,可以替代抗生素。 相似文献
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试验探讨在不同精粗比日粮中添加中草药对滩羊增重、肉理化性能、血液生化及免疫指标的影响。试验1组日粮精粗比为1.59,不添加中草药添加剂;试验2、3、4组日粮精粗比分别为1.59、2.31、0.98,且添加0.65%中草药添加剂。结果表明:各试验组间增重、日增重差异不显著(P>0.05)。试验3组增重和日增重高于试验2组和试验4组;饲喂中草药添加剂的试验2、3、4组IgA、IgG、IgM、WBC、LYM指标均高于不添加中草药添加剂的试验1组,且试验4组IgA、IgG、IgM、WBC、LYM均高于其他各组。试验90 d,试验4组与试验3组之间白细胞差异显著(P<0.05),试验30 d与试验90 d,试验3组淋巴细胞差异显著(P<0.05),试验4组淋巴细胞差异显著(P<0.05),中草药添加剂与合适的精粗比日粮可显著提高滩羊的血液生化指标和免疫水平;试验4组与试验3组间熟肉率差异显著(P<0.05),试验3组与其他各组pH值差异显著(P<0.05),且在各组中最高。说明滩羊育肥期日粮中添加0.65%中草药添加剂精粗比为0.98对其增重、肉理化性能、血液生化及免疫指标效果较好。 相似文献
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试验旨在探究复方中草药制剂对黄羽肉鸡育雏期间免疫器官指数及脾脏中IL-2、IL-4、IFN-γ因子表达的动态影响。选取1日龄健康黄羽肉鸡450只,随机分成3个处理组(对照组、抗生素组和中草药组),每个处理5个重复,每个重复30只。分别在21、35、49日龄测定鸡免疫器官指数和脾脏细胞中IL-4、IL-2、IFN-γ的动态表达量。结果显示:中草药组的免疫器官指数显著高于对照组(P<0.05),血清中免疫球蛋白IgG含量与对照组相比显著增加(P<0.05);中草药制剂组黄羽肉鸡脾脏中的IL-4 mRNA表达量在35日龄显著高于对照组和抗生素组(P<0.05),IL-2和IFN-γmRNA表达量在试验期呈增加趋势,其表达量在49日龄时中草药组显著高于对照组和抗生素组(P<0.05),说明复方中草药有提高免疫细胞因子mRNA表达量的作用。试验结果表明,基础日粮中添加复方中草药对黄羽肉鸡免疫器官发育和细胞免疫水平有不同程度的促进作用。 相似文献
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Acupuncture and traditional Chinese veterinary medicine for treatment of a mare with rectal paralysis 
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下载免费PDF全文 M. M. Sleeper K. Fishman R. J. MacKay M. Brown A. G. Bauck H. Xie 《Equine Veterinary Education》2018,30(9):460-463
An 8‐year‐old Appaloosa mare with rectal paralysis due to a cosmetic ethanol ‘tail block’ was treated with traditional Chinese veterinary medicine treatments including acupuncture and herbal medicine. Her rectal and tail tone gradually improved after the treatment. At 4 months after initial presentation, the mare was able to produce faecal piles on a regular basis, and manual evacuations were no longer needed. Significant improvement was within 30 days of beginning treatment. At 8 months, the owner indicated that the mare had normal defaecation, was able to swish the tail from side to side and lift the tail to urinate, and had no evidence of straining to defaecate or colic. 相似文献
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试验旨在研究日粮添加不同水平的中草药添加剂对母兔泌乳力影响。选取99只体重相近、胎次相同的母獭兔,随机分为3组,每组33只,分别饲喂基础日粮、基础日粮+1%中草药添加剂和基础日粮+2%中草药添加剂。预试期4 d,正试期26 d（分娩后第0天至下一次配种前1天）。检测其产仔性能及分娩第0、7、14、21和28天的泌乳量。试验结果表明,饲喂2%中草药添加剂的母兔产后不同时间泌乳量极显著（P<0.01）或显著（P<0.05）高于对照组;1%添加剂组母兔泌乳量与对照组相比有增加趋势,但差异不显著（P>0.05）;随着中草药添加量的增加,仔兔断奶成活率逐渐提高,但差异不显著（P>0.05）。综合以上试验结果,中草药添加剂有提高母兔泌乳力及仔兔断奶成活率的效果,以添加量为2%为宜。 相似文献
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This study was designed to explore the effect of Chinese herbal medicine additives on milk production and reproduction performance of rabbit does. Total 99 does were separated into 3 treatments (33 does in each treatment) and fed with the basal diet adding 0, 1%, 2% Chinese herbal medicine additives,respectively. Pre-feeding period was 4 d and test period was 26 d (from the first day of postpartum to the last day before next mating). Daily milk production of does was detected after delivery 0, 7, 14, 21 and 28 d. Both litter size and the number of weaned rabbits were recorded. The results showed that milk production of does fed with 2% Chinese herbal medicine additives were increased extremely significantly (P<0.01) or significantly (P<0.05) compared to control group, and there was no significant difference between control group and 1% Chinese herbal medicine additives group (P>0.05). Survival rate of weaned rabbits increased with the increasing dosage of the additives, but did not differ significantly (P>0.05). In conclusion, the Chinese herbal medicine additives could improve milk production of rabbit does and survival rate of weaned rabbits, and the optimal dosage should be 2%. 相似文献
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Berry WL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(6):609-612
Seven dogs with Spirocerca lupi esophageal granulomas were identified based on the site of involvement (ie, distal esophagus) and characteristic endoscopic appearance. Six dogs presented with signs of esophageal disease and 1 dog was asymptomatic. Ova were only identified in the feces of 2 dogs. On thoracic radiographs, 4 dogs had evidence of a caudodorsal mediastinal mass, and 2 of these dogs had spondylitis of midthoracic vertebrae. On endoscopy, single esophageal nodules were observed in 5 dogs, 1 dog had 3 nodules, and 1 dog had 6 nodules. All 7 dogs were treated with doramectin at a dosage of 200 microg/kg SC at 14-day intervals for 3 treatments. Dogs had physical and endoscopic examinations at 2, 4, and 6 weeks after treatment. By 6 weeks, clinical signs had resolved in 6 dogs (1 dog was asymptomatic), and the esophageal nodules had completely resolved in 4 dogs, and incompletely resolved in 3 dogs. Two dogs with incomplete resolution were treated again with doramectin at 500 microg/kg PO daily for an additional 6 weeks. Complete resolution of the esophageal nodules was confirmed by endoscopy in all dogs. Nodules had resolved in 4 dogs by 6 weeks, in 2 dogs by 12 weeks (after 6 weeks additional daily oral therapy), and in 1 dog 22 months after the initial 200 microg/kg treatment regimen. No dog experienced adverse effects to the drug, and all symptomatic dogs have been free of disease for periods ranging from 3 to 4 years. 相似文献
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试验选择756羽开产50周龄左右的罗曼褐壳蛋鸡,随机分为7组,试验1组为对照组,饲喂基础日粮;试验2组饲喂基础日粮+中草药(0.04%);试验3组饲喂基础日粮+中草药(0.04%)+酶制剂(0.01%);试验4组饲喂基础日粮+中草药(0.04%)+酶制剂(0.01%)+抗菌肽(0.1%);试验5组饲喂基础日粮+益生菌(2‰);试验6组饲喂基础日粮+益生菌(2‰)+酶制剂(0.01%);试验7组饲喂基础日粮+益生菌(2‰)+酶制剂(0.01%)+抗菌肽(0.1%)。结果表明,以试验4组效果最好,显著提高了产蛋率(P<0.05),降低了料蛋比(P<0.05),极显著提高了鸡蛋哈氏单位(P<0.01)。同时,与其他试验组相比,试验4组氨基酸总量最高,胆固醇含量最低。因此,推荐在蛋鸡日粮中添加中草药+酶制剂+抗菌肽组合对蛋鸡产蛋性能及蛋品质具有较好的效果。 相似文献
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为了探明中草药复方对雏鸡感染肠炎沙门氏菌（S. Enteritidis）的防治效果,本研究选取黄连、鹿蹄草、白头翁等23味中草药组成的复方中草药制剂进行体外和体内抑菌试验。体外抑菌试验中草药复方的抑菌直径为17.4 mm,说明沙门氏菌对其高度敏感。体内抑菌试验中,将中草药复方以低（0.1%）、中（0.3%）、高（0.5%）3个剂量进行治疗试验,结果表明,肠炎沙门氏菌感染后,与对照组相比,3个试验组均极显著提高了试验鸡的成活率（P<0.01）,显著降低了试验鸡的料重比（F/G）（P<0.05）,中、高剂量组的平均日增重（ADG）显著高于对照组（P<0.05）,中剂量组的平均日采食量（ADFI）显著低于对照组（P<0.05）。3个试验组试验鸡的脾脏指数、白细胞数量（WBC）、血小板计数（PLT）、免疫球蛋白A（IgA）、IgG、IgM、补体C4含量均显著低于对照组（P<0.05）,而胸腺指数、法氏囊指数、红细胞数量（RBC）、血红蛋白浓度（HGB）、红细胞压积（HCT）均显著高于对照组（P<0.05）。实时荧光定量PCR结果显示,抗菌肽基因（CATH1、CATH2、CATH3）在脾脏、胸腺和法氏囊中的表达量均不同程度地高于对照组。综合试验结果,日粮中添加中草药复方可以降低雏鸡感染肠炎沙门氏菌后的死亡率,提高雏鸡的免疫能力和抗菌能力,改善生长性能,其中,中剂量组（0.3%）的防治效果最佳。 相似文献
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中草药饲料添加剂对母羊繁殖性能的影响 总被引：3，自引：0，他引：3
试验设3个处理组（A组为中药组、B组为中草药＋激素组、C组为激素组）,于母羊配种开始时将中草药添加于饲料中,每只羊每天25克,连用20 d,C组不添加。结果表明：1）A组使阴道涂片上皮细胞变化明显,间质细胞最终转化成角化细胞;使母羊发情并排卵,但排卵作用较弱;使处于非繁殖季节母羊于用药9 d后进入发情前期,用药15 d后进入发情期,并且出现发情外观变化;可有效诱导非繁殖季节羊的发情,并且阴道细胞学变化明显;诱导非繁殖季节母羊发情时,发情率达到80%,受胎率仅有25%。2）B组可有效诱导非繁殖季节母羊发情,效果显著,发情率达87.5%,受胎率高到85.5%。 相似文献
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目的 研究旨在通过试验观察中草药治疗奶牛子宫内膜炎疗效,以其为临床应用中草药治疗奶牛子宫内膜炎提供参考。方法 将60 头患子宫内膜炎奶牛,随机分成5 组,每组12 头,复方中草药高、中、低剂量组（分别子宫灌注150 mL/头、100 mL/头和50 mL/头）、土霉素组（子宫灌注50 mL/头）和对照组。结果 复方中草药中剂量组和高剂量组的总有效率为75.00%和83.33%,均高于土霉素组的总有效率66.67%;土霉素组在治疗结束后的平均发情天数缩短了14 d,所有复方中草药组的发情天数也缩短了11~19 d;3 个情期内土霉素组受胎率为50.00%,复方中草药组受胎率介于41.67%~66.67%之间。结论 复方中草药对奶牛子宫内膜炎的治疗效果优于土霉素,其中以高剂量组效果最佳。 相似文献
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试验旨在探讨黄柏、诃子、知母、苏木等常用中草药对水产动物致病菌的体外抑菌效果,为今后水产养殖细菌性疾病的防控提供参考。以美人鱼发光杆菌、嗜水气单胞菌、哈维氏弧菌、创伤弧菌临床分离株为试验菌株,以抑菌圈直径、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）及联合抑菌指数（FICI）作为检测指标,研究常用中草药对水产病原菌的抑菌活性。采用水提法提取中草药有效成分,并通过琼脂平板打孔法测定中草药对水产致病菌抑菌圈,微量肉汤稀释法测定中草药MIC和MBC及微量肉汤棋盘稀释法测定联合抑菌指数（FICI）。结果显示,16种中草药对水产致病菌具有不同程度的抑菌作用。药敏试验结果显示,苏木、女贞子、夏枯草、青皮对4种水产致病菌均具有抑菌效果,抑菌圈直径在10.5~29.0 mm之间,而4种病原菌对马齿苋均表现为耐药;MIC检测结果显示,苏木、女贞子、夏枯草、青皮对4种病原菌的MIC值为7.81~250.00 mg/mL;MBC检测结果显示,苏木、女贞子、夏枯草、青皮对4种病原菌的MBC值为7.81~500.00 mg/mL;联合抑菌试验结果显示,苏木与夏枯草、女贞子及青皮联用仅对美人鱼发光杆菌起到协同抑菌作用,对嗜水气单胞菌、创伤弧菌及哈维氏弧菌均无抑菌作用,女贞子和青皮组合仅对哈维氏弧菌抑菌作用为相加作用,该组合在抑菌效果上对其他病原菌表现为无关或颉颃作用,其他药物组合在抑菌效果上对4种病原菌则表现为无关或颉颃作用;联合抑菌圈测定结果表明,中草药联合使用对4种病原菌抑菌作用不一。本试验结果表明常用中草药对水产动物致病菌具有一定程度的抑菌作用。 相似文献
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选用体质健康,体重、产蛋率基本一致的60周龄海蓝白蛋鸡250只,随机分为5组:中药复方Ⅰ、Ⅱ、Ⅲ组、电解多维组、高温对照组。本试验检测蛋鸡肺脏中钙含量,并采用ELISA法测定蛋鸡肺脏组织热休克蛋白70(HSP70)表达量。结果显示,复方中药能够提高肺脏钙离子(Ca2+)浓度和HSP70表达量。复方Ⅰ组能明显提高Ca2+浓度。各药物组的肺脏HSP70含量均高于高温对照组,其中复方Ⅲ组最为明显,但是复方Ⅰ组对HSP70的增加效果最显著。结果表明,HSP70与钙含量变化呈一定的正相关,中药有可能通过提高钙离子浓度而实现对HSP70的调控。 相似文献
18.
选择45头体况良好60日龄左右杜长大三元杂交健康仔猪,分为3组,每组设3个重复,每组重复5头。各组添加药物成分,检测其血液指标的变化。A组添加0．5％中药制剂,B组添加0．5％木醋液,C组添加0．5％中药＋木醋液（1：1）。试验结果表明,淋巴细胞A、B、C3组之间差异极显著（P〈0．01）;谷草转氨酶、谷丙转氨酶A、C与B组之间差异极显著（P〈0．01）,总蛋白、白蛋白、球蛋白A组、C组与B组之间差异极显著（P〈0．01）,甘油三酯、总胆固醇B组与A组、C组之间差异极显著（P〈0．01）,这说明A、C两组在提高动物机体对营养物质消化吸收和代谢率、提高育肥猪免疫力方面优于B组,而B组在降低胆固醇和甘油三酯方面好于A、C两组。 相似文献
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David F. Edwards DVM Thomas G. Nyland DVM Joseph P. Weigel DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1988,2(4):184-191
The clinical, laboratory, radiographic, and histologic features and the response to therapy in three dogs with actinomycosis are reported. One dog (dog 1) had a 12-cm nonresectable mass extending from the ventrolateral chest wall into the left ventricular myocardium. Another dog (dog 2) had a diffuse peritonitis with "sulfur granules" and two large masses. One of these masses was nonresectable involving adjacent abdominal structures. A third dog (dog 3) had a subvertebral mass at T1-3 producing quadraplegia. Two dogs had periosteal reactions involving adjacent sternebrae (dog 1) or ribs and vertebral bodies (dog 3) that are characteristic of Actinomyces spp infections. In dogs 1 and 2 the diagnosis was based on the morphologic and tinctorial properties of free sulfur granules and/or tissue granules. Culture results were variable. Tissue from dog 1 yielded no growth, while polymicrobial infections, which included Actinomyces spp, were identified in dogs 2 and 3. Actinomyces odontolyticus was isolated from dog 3. Although the actinomycotic granulomas were either not excised or only partially excised from dogs 1 and 2, both animals were cured by the oral administration of high doses of penicillin G for 19 and 6 months, respectively. Dog 3 responded dramatically to the same antibiotic therapy given for 5 months. However, within 4 months of discontinuing treatment an abscess and draining fistulous tracts developed in the left axillary region. Two surgical fistulectomies and additional penicillin therapy were required to cure this animal. These cases and the current veterinary and human literature on actinomycosis are used to propose a rational approach to the treatment of actinomycosis in the dog. 相似文献