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1.
利用超声波法提取野生薄荷精油,针对总抗氧化能力、超氧阴离子自由基清除能力、羟基自由基清除能力及DPPH自由基清除能力薄荷精油的抗氧化活性进行研究,同时与合成抗氧化剂没食子酸丙酯(PG)的抗氧化性进行比较分析。结果表明,新疆伊犁野生薄荷精油是一种天然的抗氧化剂,并呈现一定的量效关系,其抗氧化能力在各方面均比人工合成的抗氧化剂PG要弱,对清除DPPH自由基的能力远不如人工合成的抗氧化剂PG,而在对清除超氧阴离子自由基能力和清除羟基自由基能力上具有显著的能力与效果。  相似文献   

2.
以薏苡仁为原料,研究薏苡仁多糖对超氧阴离子自由基(O_2·)、羟基自由基(·OH)及DPPH自由基的抗氧化清除能力。将VC的抗氧化能力作为参照,研究多糖质量浓度与抗氧化能力的关系。结果表明,当质量浓度为5 mg/m L时,对超氧阴离子自由基(O_2·)、羟基自由基(·OH)以及DPPH自由基的抗氧化清除能力分别为44.81%,10.12%,29.25%,且随着薏苡仁多糖质量浓度的增加其抗氧化能力越强。  相似文献   

3.
核桃青皮多酚的抗氧化性研究   总被引:3,自引:0,他引:3  
通过体外抗氧化体系对核桃青皮多酚的抗氧化能力进行研究。结果表明,核桃青皮多酚在各体系中均有一定的抗氧化性能,并且其抗氧化能力随着浓度的增加而增强;在所选浓度下,核桃青皮多酚对DPPH自由基和过氧化氢的清除能力、对三价铁离子的还原能力均明显强于VC。当其质量浓度高于10 mg/L时,核桃青皮多酚对羟基自由基的清除作用强于VC对超氧阴离子自由基的清除作用;当其质量浓度低于22.3 mg/L时,核桃青皮多酚对超氧阴离子自由基的清除力强于VC对超氧阴离子自由基的清除作用。  相似文献   

4.
本试验对山丹鳞茎中总黄酮的抗氧化性和抑菌性进行研究。试验利用三种体外抗氧化体系即超氧阴离子清除能力(O_2~-)、羟基自由基(OH)清除能力和二苯基苦基苯肼自由基(DPPH)清除能力对山丹鳞茎总黄酮的体外抗氧化活性进行了综合评价,采用打孔法测定其抑菌活性。试验显示山丹鳞茎总黄酮对O_2~-的清除率为63.27%,对OH的清除率为70.36%,对DPPH的清除率为52.83%,在试验浓度范围内优于BHT的清除能力,并对9种菌均有不同程度的抑制作用,说明山丹鳞茎总黄酮适宜作为一种天然的抗氧化剂和抑菌剂而广泛利用。  相似文献   

5.
羊栖菜中多酚的提取制备及体外抗氧化活性研究   总被引:2,自引:0,他引:2  
为研究羊栖菜多酚的提取工艺及其体外抗氧化活性,在单因素试验的基础上,以多酚浸出率为响应值,采用响应面法优化羊栖菜多酚提取工艺,并考察其对DPPH自由基、超氧阴离子自由基、羟自由基的清除能力以及总抗氧化能力。结果表明:在温度为70℃,乙醇浓度为40%,浸提时间46 min,液料比20:1,羊栖菜多酚的浸出率为4.048%;粗提液经XDA-7大孔树脂柱层析分离纯化其纯度为61.86%;羊栖菜多酚对DPPH自由基、超氧阴离子自由基、羟自由基清除作用的IC50分别为6.65、6000、125.42μg/m L,总抗氧化能力为65.04 U/mg。该结果表明:羊栖菜多酚是一种极具开发潜力的天然抗氧化剂。  相似文献   

6.
本实验对山丹鳞茎中总皂苷的抗氧化性和抑菌活性进行研究。实验利用三种体外抗氧化体系即羟基自由基(OH)清除能力、超氧阴离子清除能力(O2-)和二苯基苦基苯肼自由基(DPPH)清除能力对山丹鳞茎总皂苷的体外抗氧化活性进行了综合评价,采用滤纸片法测定其抑菌活性。实验表明:在实验浓度范围内,其对OH的清除率为59.68%,O2-的清除率为40.58%,对DPPH的清除率为76.06%,并对10种菌均有不同程度的抑制作用,说明山丹鳞茎总皂苷适宜作为一种天然的抗氧化剂和抑菌剂而广泛利用。  相似文献   

7.
采用热水浸提法分别在不同提取温度(40,60,80,100℃)下提取得到4种蛤蒌叶粗多糖,并测定其总多酚和蛋白质的含量。然后测定4种粗多糖对羟基自由基、DPPH自由基和超氧阴离子自由基的清除能力,以及粗多糖的还原能力来评价提取温度对其抗氧化活性的影响。结果显示,蛤蒌叶粗多糖中总多酚和蛋白质的含量随着提取温度的升高而下降。提取温度在40~100℃内,随着提取温度的升高,蛤蒌叶粗多糖对羟基自由基、DPPH自由基和超氧阴离子自由基的清除能力均呈下降趋势,而提取温度对其还原能力的影响没有明显差别。结果表明,提取温度对于蛤篓叶粗多糖的抗氧化活性有显著影响,主要是通过影响粗多糖中的总多酚和蛋白质含量来影响其抗氧化活性。  相似文献   

8.
鱼腥草挥发油成分分析及其抗氧化性研究   总被引:1,自引:0,他引:1  
吕都  刘嘉  刘辉  王辉  李俊  刘永翔 《保鲜与加工》2016,16(6):120-124
以鱼腥草挥发油为研究对象,采用气相色谱-质谱联用仪(GC-MS)测定其成分和相对百分含量,并就鱼腥草挥发油对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟自由基(·OH)和超氧阴离子自由基(O_2~-·)的清除能力进行研究,同时与合成抗氧化剂没食子酸丙酯的抗氧化能力进行比较。结果表明,鱼腥草挥发油中的主要成分为萜类化合物、酯类化合物、烯烃、醇类化合物等;鱼腥草挥发油是一种天然的抗氧化剂,随浓度的升高,其抗氧化能力逐渐增强,呈现一定的量效关系,但其抗氧化能力低于没食子酸丙酯溶液;同等浓度下,鱼腥草挥发油对3种自由基的清除能力由大到小为:DPPH··OHO_2~-·。  相似文献   

9.
以废弃竹笋壳所提取的木质素为原料,乙酸方法用来处理竹木质素和木质素磺酸盐的制备,对所提取的木质素抗氧化活性进行了研究。木质素DPPH自由基清除指数(RSI)值为1.63,远高于商业合成抗氧化剂二丁基羟基甲苯(BHT) RSI值(0.84),作为天然抗氧化剂具有一定的开发利用价值。比较抗坏血酸(维C)和商业木质素磺酸钠与制备的木质素磺酸钠,结果表明,同一浓度条件下,对亚硝酸根(NO_2~-)的清除能力依次为自制木质素磺酸钠商品木质素磺酸钠维C,对羟基自由基(·OH)的清除能力依次为维C商品木质素磺酸钠自制木质素磺酸钠,对超氧阴离子(O_2~-)的清除能力依次为自制木质素磺酸钠商品木质素磺酸钠维C,对二苯代苦味酰基自由基(DPPH·)的清除能力依次为维C自制木质素磺酸钠商品木质素磺酸钠,说明在一定程度上,笋壳木质素具有广泛应用于食品及其他工业作为抗氧化剂的潜力。  相似文献   

10.
为研发优质葛根汁及葛根饮料产品,以葛根为试材,通过对比试验和感官评价,确定葛根汁及葛根汁饮料生产工艺过程和产品配方,通过抗氧化试验,评价葛根汁及葛根饮料对羟基自由基、超氧阴离子自由基、DPPH自由基的清除能力及总还原能力。结果表明,鲜葛根经清洗、破碎和打浆,于90℃,30 min条件下糊化,用α-淀粉酶在90℃条件下液化2 h,再用β-淀粉酶于60℃条件下糖化2 h,经过滤压榨后获得的葛根汁品质最好,葛根汁中黄酮含量为0.45 mg/m L。对比试验优选的葛根饮料黄酮含量为0.20 mg/m L;葛根原汁与葛根饮料对DPPH自由基清除能力接近,对比羟基自由基和超氧阴离子自由基的清除能力,葛根汁远高于葛根饮料。  相似文献   

11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans.  相似文献   

12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes.  相似文献   

13.
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol...  相似文献   

14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed.  相似文献   

15.
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献   

16.
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination...  相似文献   

17.
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums.  相似文献   

18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf 1) and recessive for the other (Rf 2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph 3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf 1(one of two dominant additive genes controlling early season leafing out) indicated that Rf 1and Rf 2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf 1, and 0.15 for Lf 1-Sph 3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph 3.It is suggested that competitive disadvantage of lf 1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph 3. Poor performance of lf 1- (and possibly lf 2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off).  相似文献   

19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho...  相似文献   

20.
T. Visser  E. H. Oost 《Euphytica》1981,30(1):65-70
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed.  相似文献   

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