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1.
利用进口FSH超排12月龄的波尔母山羊6只,获卵83枚,只均13.8枚,获可用胚74枚,最高一只19枚,只均12.33枚。共移植奶山羊受体37只,两个情期不返情27只,情期不返情率72.97%。35~40d用B超探测仪检查怀孕结果(以观察到胎儿为准),实际怀孕22只,移植妊娠率59.46%,其中移植双胚的妊娠率66.67%,单胚的妊娠率50.00%。  相似文献   

2.
本试验对中国美利奴成年母羊60只,幼龄母羊(8月龄)19只。用同期发情处理。其中,A组17只成年母羊发情配种。发情率为100%,双胎率为73.3%;B组43只成年母羊同期处理后,利用腹腔内窥镜观察卵巢排卵情况。其中排双卵的羊35只,占81.4%,明显高于自然发情母羊双胎率(23.3%)(P〈0.01);C组同期处理8月龄母羊19只,配种后妊娠14只,其中以胎6只,双胎率为31.6%。  相似文献   

3.
2003年5月和2004年6月分两批从澳大利亚引进三个绵羊品种:道塞特(Dorset)、萨福克(Suffork)和特克塞尔(Texel)共389只,选择其中116只作为供体母羊进行胚胎移植,使甩CIDR+FSH递减法对供体超数排卵处理,其中111只供体发情,配种、采胚(反应率95.6%,111/116),平均采胚10.04枚(1114/111),可用胚平均6.23枚(691/111),可用胚率62.03%(691/1114)。将691枚2~16细胞可用胚移植给618只受体小尾寒羊,受体采用PG两次处理法同期处理。妊娠485只,妊娠率78.48%,共产羔537只,产羔率110.72%。  相似文献   

4.
试验于2004年4月8日开始,随机选择12只夏洛莱羊作为供体,当地普通绵羊100只作为受体,进行诱导发情处理和超排处理。供体诱导发情率83.3%(10/12),受体诱导发情率88%(88/100)。回收卵128枚,其中获得有效胚胎96枚、未受精卵32枚。移植妊娠率64.8%(57/88)。  相似文献   

5.
波尔山羊冷冻胚胎移植试验   总被引:1,自引:0,他引:1  
2003年5月至2004年7月在沿河县歇凉坪、肖家坝两个养羊场实施了波尔山羊冷冻胚胎移植项目,并取得了成功。试验应用孕酮海绵栓埋法,结合注射苯甲酸雌二醇对153只沿河山羊进行了诱导同期发情处理,结果在24~48小时内发情141只.同期发情率为92.16%,其中有72.34%的母羊集中在36~48小时内发情。发情母羊中检出黄体合格母羊90只,合格率为63.83%;对90只黄体合格母羊移植波尔山羊冻胚,借腹怀胎,经两个情期后检查妊娠48只,移植妊娠率53.33%,高于项目计划50%的指标。  相似文献   

6.
冲取32只超排或自然发情配种的母兔早期胚胎427枚,选其中导入抗猪瘟Ribozyme基因后的347枚优质胚胎分组移植不同受体。第一组将93枚微注射卵移植到7只冲卵后的供体输卵管内,6只妊娠并产仔32只。经PCR检测32只仔兔中有2只整合了外源基因。第二组将254枚微注射卵移植异体受体13只,妊娠6只并产仔20只。经PCR检测20只仔兔中有2只整合了外源基因。  相似文献   

7.
布尔山羊超数排卵胚胎移植试验   总被引:2,自引:0,他引:2  
本试验采用置入炔诺酮阴道栓 FSH 氯前列烯醇同期发情、超数排卵处理方法,对8只布尔山羊(供体羊)、90只洪洞奶山羊(受体羊)进行了同期发情和超排数排卵胚胎移植试验。其结果如下:(1)8只供体羊回收胚胎数量、可用胚胎数量、羊均回收胚胎数量、羊均可用胚胎数量、可用胚比率分别为182枚、172枚、22.8枚、21.5枚、94.5%;(2)90只受体羊共有81只羊达到同期发情效果,发情同期率为90.0%;(3)150枚可用胚胎共移植洪洞奶山羊73只,妊娠51只,产羔80只,妊娠率69.9%,胚胎成羔率53.5%。  相似文献   

8.
胚胎移植在奶牛生产中的推广应用研究   总被引:5,自引:0,他引:5  
3年共计超排供体高产奶牛68头次,获卵57头次,获卵率为83.82%;共获卵537枚,每头供体平均7.89枚,共获可用胚372枚,头平获可用胚5.47枚。移植低产奶牛受体253头,妊娠121头,移植妊娠率为48%。共产犊111头,其中母犊53头。  相似文献   

9.
利用进口FSH超排12月龄的波尔母山羊6只,获卵83枚 ,只均13.8枚,获可用胚74枚,最高一只19枚,只均12.33枚。共移植奶山羊受体37只,两 个情期不返情27只,情期不返情率72.97%。35~40 d用B超探测仪检查怀孕结果(以观 察到胎儿为准),实际怀孕22只,移植妊娠率59.46%,其中移植双胚的妊娠率66.67%,单胚 的妊娠率50.00%。  相似文献   

10.
我们于1988年10至11月份,在本院进行了绵羊胚胎冷冻保存及冻胚移植试验。使用国产FSH和LH对5只母羊做了超数排卵处理,共排卵82枚(以黄体数计),平均每只排卵16.4±3.6枚,采用手术法收集胚胎38枚,采卵率为46.3%。其中可利用胚胎32枚,占回收胚胎数的84.2%。超排母羊头均采可用胚胎6.4枚。使用日本产计算机控制的胚胎冷冻装置冷冻胚胎22枚。解冻后存活胚胎12枚,存活率为54.5%。将10枚冻胚手术移植给5只受体母羊。现有2只受体母羊返情,情期不返回率为60%。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

20.
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