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1.
为了将马铃薯提取淀粉的废弃物——马铃薯渣变废为宝,利用联合酶解法提取薯渣中的膳食纤维。分别通过单因素试验和正交试验来确定α-淀粉酶和糖化酶联合酶解法提取膳食纤维的最佳工艺条件。首先,在保证糖化酶酶解工艺条件不变的情况下,以膳食纤维百分含量为评价指标,利用单因素试验和正交试验确定提取马铃薯渣膳食纤维α-淀粉酶的工艺条件;然后,利用确定的条件进行α-淀粉酶酶解,再利用单因素试验和正交试验确定糖化酶酶解的最优工艺条件。确定的酶联法提取膳食纤维的最优工艺条件为先添加300 U/g的α-淀粉酶(酶解时间60 min,酶解温度55℃,p H值6.5);灭活酶后,再利用糖化酶进行酶解,添加250 U/g的糖化酶酶解(酶解时间30 min,酶解温度65℃,p H值4.0)。在最佳组合条件下,试验取平均值得到膳食纤维百分含量为76.92%,同时提取后的膳食纤维其持水性和持油性显著高于马铃薯渣。  相似文献   

2.
以大豆为原料,研究双螺杆挤压机各参数对大豆膳食纤维可溶性的影响,并以水溶性膳食纤维的含量为指标,通过单因素及正交试验筛选出最佳工艺条件:物料粒度50目,物料含水量16%,膨化温度140℃,螺杆转速450 r/min。该条件下水溶性膳食纤维质量分数为21.35%。  相似文献   

3.
制备高品质的沙果渣膳食纤维,以沙果渣为原料,通过单因素试验和正交试验,研究纤维素酶酶解法制备高活性沙果渣膳食纤维的最佳工艺条件,并对优化后沙果渣膳食纤维的持水力、持油力和膨胀力等理化性质进行了分析。结果表明,最佳工艺条件为纤维素酶用量50 U/g,酶解时间80 min,酶解温度45℃,pH值4.6,此时测得SDF/TDF为18.86%。该条件下所得的膳食纤维呈淡黄色,其持水力为6.87 g/g,持油力为7.36 g/g,膨胀力为6.54 mL/g。  相似文献   

4.
以蕨菜为试验材料,研究超声波辅助酸法提取蕨菜中水溶性膳食纤维的影响因素,通过单因素试验和正交试验确定提取的最佳工艺参数。结果表明,影响蕨菜中水溶性膳食纤维得率的因素主次顺序为提取温度超声功率料液比柠檬酸质量分数,最佳提取的工艺条件为提取时间60 min,料液比1∶19 (g∶mL),超声功率180 W,柠檬酸质量分数2%,提取温度60℃,实际测得水溶性膳食纤维得率为5.76%。  相似文献   

5.
苜蓿营养丰富,富含叶蛋白、叶黄素及膳食纤维。以"肇东"紫花苜蓿弃用粗茎为原料,采用酸热法提取苜蓿水溶性膳食纤维。研究水浴时间、水浴温度、酸液浓度和酸液用量对苜蓿水溶性膳食纤维得率的影响。利用正交设计试验,确定了最佳提取工艺,其最佳工艺参数为:加热时间100 min,加热温度80℃,酸液体积分数3%,酸液用量60 mL。在该最佳条件下,苜蓿水溶性膳食纤维的得率为6.46%。  相似文献   

6.
葵花子皮中水溶性膳食纤维初步研究   总被引:2,自引:1,他引:1  
为了提高葵花子皮中水溶性膳食纤维得率,在单因素实验基础上,通过响应面法对水溶性膳食纤维提取工艺进行优化研究。结果表明:提取瓜子皮中水溶性膳食纤维的最佳工艺参数为NaOH的质量分数为7.83%、提取时间为69.46 min、提取温度为42.10℃、液料比为40 mL/g;在最优工艺条件下水溶性膳食纤维的得率可达31.1112%,同时经测定得知葵花子皮膳食纤维具有良好的持水性和溶胀性:持水性为7.293 g/g,溶胀性为3.997 mL/g。因此瓜子皮可以作为提取水溶性膳食纤维的良好来源。  相似文献   

7.
以荔枝渣为原料,采用酶-化学提取技术从荔枝渣中提取、纯化膳食纤维。在单因素试验基础上,通过正交试验优化酶-化学法的工艺条件,再把提取、纯化所得的荔枝渣膳食纤维进行物化性质的测定。结果表明,提取、纯化荔枝渣膳食纤维的最佳工艺条件是当α-淀粉酶质量分数为0.4%,酶处理温度为65℃,酶处理时间为75 min,碱液质量分数为0.25%,碱液处理温度为60℃,碱液处理时间为30 min时,提取率最高为36.35%;物化性质测定结果显示膨胀率为1.64 mL/g,持水率为4.86,且膨胀率和持水性都较荔枝壳膳食纤维好。  相似文献   

8.
采用化学法从光皮木瓜渣中提取水不溶性膳食纤维,对液料比、Na OH浓度、提取温度、提取时间4个因素进行单因素试验,利用正交试验确定最佳提取工艺条件。结果表明:光皮木瓜渣中水不溶性膳食纤维提取的最佳工艺条件为:液料比20∶1(m L/g),Na OH浓度0.75 mol/L,提取时间70 min,提取温度60℃,在此工艺条件下的提取率为25.229%。  相似文献   

9.
以柚子皮为原料,采用超声辅助酶法提取柚子皮中水溶性膳食纤维,探讨料液比、加酶量、超声时间对提取率的影响。在单因素试验的基础上,通过正交试验优化工艺条件,并对其抗氧化活性进行研究。结果表明,超声辅助酶法提取柚子皮水溶性膳食纤维的最佳工艺条件为:加酶量400μL,料液比1∶16(g/m L),超声时间25 min,在该工艺条件下的平均提取率为4.67%。浓度为2 mg/m L的柚子皮水溶性膳食纤维对超氧阴离子自由基和羟自由基的清除率分别为(11.72±1.41)%和(40.79±4.06)%,对铁的还原能力为(0.128 3±0.004 7)mmol/g,表现出较好的抗氧化活性。  相似文献   

10.
酶法提取豆渣水溶性膳食纤维的研究   总被引:1,自引:0,他引:1  
为了提高豆渣水溶性膳食纤维的得率,通过正交试验确定了提取水溶性膳食纤维的最佳工艺。研究结果表明,当纤维素酶添加量为4%,加水量为25mL/g,反应时间为2h,反应温度为50℃,反应pH值为5时,水溶性膳食纤维得率为13.7%,达到最大。  相似文献   

11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans.  相似文献   

12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes.  相似文献   

13.
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol...  相似文献   

14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed.  相似文献   

15.
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献   

16.
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination...  相似文献   

17.
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums.  相似文献   

18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf 1) and recessive for the other (Rf 2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph 3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf 1(one of two dominant additive genes controlling early season leafing out) indicated that Rf 1and Rf 2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf 1, and 0.15 for Lf 1-Sph 3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph 3.It is suggested that competitive disadvantage of lf 1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph 3. Poor performance of lf 1- (and possibly lf 2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off).  相似文献   

19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho...  相似文献   

20.
T. Visser  E. H. Oost 《Euphytica》1981,30(1):65-70
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed.  相似文献   

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