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1.
用弓形虫的重组蛋白GST-SAG1作为ELISA诊断抗原,采用ELISA检测方法,对青海省祁连县喜马拉雅旱獭血清进行了弓形虫病的血清学检测。结果表明,被检92份喜马拉雅旱獭共检出阳性血清25份,阳性率约为27.17%。结果提示,青海省祁连县的喜马拉雅旱獭中存在弓形虫病的感染。 相似文献
2.
应用ELISA诊断方法,对来自青海省的部分孕妇、藏系绵羊、马、藏狗、骆驼、喜马拉雅旱獭、高原鼠兔、中华鼢鼠、藏羚羊和实验小鼠的1 090份血清样品进行弓形虫特异性抗体的检测。结果检出75份阳性血清,阳性率为6.88%。 相似文献
3.
应用间接血凝试验(IHA),对青海省部分地区的藏獒、喜马拉雅旱獭、高原鼠兔及中华鼢鼠的311份血清样品,进行弓形虫特异性抗体的检测。结果检出33份阳性血清,阳性率为10.61%。 相似文献
4.
应用弓形虫的间接血凝试验（IHA）,对来自青海省境内的部分土种绵羊、马属动物、藏獒、骆驼、喜马拉雅旱獭、高原鼠兔、中华鼢鼠、藏羚羊和实验小鼠等动物的1039份血清样品,进行弓形虫特异性抗体的检测。结果检出68份阳性血清,阳性率为6.54%。 相似文献
5.
用弓形虫的重组蛋白SAG1作为ELISA诊断抗原,建立了ELISA诊断试剂盒,并对青海省牛群进行弓形虫病的血清学诊断。经对所收集的1856份牛血清进行弓形虫抗体检测,共检出阳性血清71份,阳性率约为3.83%。结果表明青海省牛群中存在弓形虫病的感染。 相似文献
6.
采集青海省都兰县境内动物血清:野生马鹿15份,野生岩羊6份,野生黄羊血清20份,高原鼠兔100份,喜马拉雅旱獭20份进行布鲁菌病检测,结果:被检野生马鹿血清15份,试管凝集阳性反应的2份,占13.3%,虎红凝集试验阳性反应1份,占6%;被检野生岩羊6份,试管凝集阳性反应1份,占16.7%,虎红凝集试验为阴性反应;被检野生黄羊血清20份,试管凝集阳性反应的3份,占15%,虎红凝集试验阳性反应1份,占5%;被检高原鼠兔血清100份,试管凝集阳性反应的3份,占3%,虎红凝集试验有阳性反应1份,占1%;被检喜马拉雅旱獭20只,试管凝集阳性反应的1只,占5%,虎红凝集试验为阴性反应。 相似文献
7.
用弓形虫的重组蛋白P30作为ELISA诊断抗原,进行了ELISA诊断试剂盒的建立,并对互助县猪弓形虫病的血清学诊断。经对所收集的139份猪血清抗体进行检测,共检出阳性血清8份,阳性率为5.76%。结果说明互助县猪群中存在弓形虫病的感染。 相似文献
8.
分析新疆维吾尔自治区巴音郭楞蒙古自治州若羌县阿尔金山国家级自然保护区喜马拉雅旱獭夏季食物组成及营养生态位,为喜马拉雅旱獭科学管理提供基础资料。2012年7~8月,对采集的喜马拉雅旱獭60份粪样组合为10个复合样本进行粪便显微组织分析,同时将研究区域采集的植物标本进行鉴别,之后通过显微镜对比分析确定食物组成及比例,并计算生态位宽度。喜马拉雅旱獭夏季采食11科18属20种植物,按科统计,禾本科(24.55%)、莎草科(17.82%)、豆科(16.31%)和菊科(10.57%)是喜马拉雅旱獭的主要食物。参考装片集合的46种植物的大约一半(43.48%)出现在粪样中。喜马拉雅旱獭夏季的食物以禾草和杂类草为主。 相似文献
9.
用试管凝集法(SAT)、间接血凝抑制试验(IHA)和酶联免疫吸附试验(ELISA)三种方法,对来自青海省湟中县田家寨镇某奶牛场发生过流产群的95份奶牛血清,进行布氏杆菌病、衣原体病和弓形虫病的血清学调查。结果:检出布氏杆菌病阳性血清1份,衣原体阳性血清15份,弓形虫阳性血清2份,血清阳性率分别为1.05%、15.79%和2.11%。初步说明引起该牛场流产的病因为布氏杆菌病、衣原体病及弓形虫混合感染。 相似文献
10.
<正>为了解青海省部分地区牛羊弓形虫病的感染情况,并为该地区预防和治疗弓形虫病提供科学依据,笔者于2010年3至5月应用间接血凝试验(IHA)对青海省泽库县的牛羊进行弓形虫病的血清学调查,现报告如下。1材料与方法1.1被检血清牛105份、羊97份被检血清均采集青海省泽库县,血样采自牛羊的颈静脉,摆成斜面,置4℃冰箱5 h后,放在室温下待血清析出后收集血清,冷冻保存待检。1.2诊断液弓形虫IHA抗原、阴性标准血清、阳性标准血清、稀释液,均购自中国农科院兰州兽医研究所。 相似文献
12.
Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
13.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
14.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
15.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
17.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
19.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
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