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B型副鸡嗜血杆菌的分离鉴定 总被引：11，自引：2，他引：9
从辽宁某公司鸡场疑似鸡传染性鼻炎的病鸡眶下窦分离到一株副鸡嗜血杆菌，用Page程序和Kume程序对其进行血清型鉴定，确认为B型副鸡嗜血杆菌，这是首次在我国分离到B型副鸡嗜血杆菌。 相似文献
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从国外引进的鸡败血支原体R株和副鸡嗜血杆菌221株和668株的冻干品,经我所扩增复壮和系统的鉴定试验证明:鸡败血支原体R株属典型的强毒株,副鸡嗜血杆菌221和668株分别属典型的A型和C型强毒株.经过免疫原性试验、毒力鉴定试验及效力试验证明,这三种菌株是制备鸡败血支原体、鸡传染性鼻炎二联疫苗最好的菌株. 相似文献
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锦州地区鸡传染性鼻炎的流行病学调查 总被引：1，自引：0，他引：1
对发生过鸡传染性鼻炎的8个鸡场,从病史调查、现场调查、临床检查、病理剖检、细菌分离鉴定、血清定型、动物致病性试验、药物敏感试验以及防治等方面进行了较为详细的研究。结果表明,锦州地区鸡传染性鼻炎的发病率为5.2%～40.7%,死亡率约为1%～3%。分离获得8株副鸡嗜血杆菌,血清型主要为A型,个别为C型,未发现B型。不同时间分离的副鸡嗜血杆菌对抗菌药物的敏感性不同。 相似文献
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为确定某规模化鸡场疑似鸡传染性鼻炎病例的病原,试验从同群发病鸡的眶下窦采样进行细菌性病原分离,并进行PCR和生化试验鉴定,利用A、B、C 3个型标准分型血清对疑似分离菌株进行HI分型,用纸片扩散法（K-B法）对分离菌株进行药物敏感性试验,并对分离菌株进行16S rRNA核苷酸遗传进化分析。结果显示：分离的菌株均为副鸡禽杆菌,这些分离株包括A型和C型两种血清型,两种血清型的分离菌株具有较强的致病性,对21种治疗畜禽疾病常用抗菌药物的敏感性也不完全相同;两个血清型分离菌株亲缘关系较远,A型与澳大利亚某株副鸡禽杆菌亲缘关系很近,C型与部分国内副鸡禽杆菌菌株亲缘关系接近。研究结果提示在该规模化养殖场发生鸡传染性鼻炎的鸡群中,同时存在2种不同血清型的副鸡禽杆菌,加大了防控的难度。 相似文献
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一鸡传染性鼻炎1病原及流行特点本病是由副鸡嗜血杆菌引起的一种鸡的急性上呼吸道传染病。副鸡嗜血杆菌属于革兰氏阴性菌,有A、B、C三个血清型。前几年,我国流行的血清型主要是A型和零星C型,但近年来主要血清型是A和B型,且A型已经出现变异,发病比例有明显增加的趋势。 相似文献
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2011年11月,山东某肉鸡场发生以肉鸡眼睑及眶下窦周围明显肿胀为主要特征的传染性疾病。从采集的发病鸡眶下窦中分离到1株细菌,根据发病鸡群的临床症状、细菌的分离培养、形态观察、过氧化氢酶试验、革兰氏染色、动物回归试验、免疫攻毒试验和PCR等方法初步鉴定为副鸡嗜血杆菌(Hpg)。此外通过分离菌株的水平传播试验结果表明,造成该鸡场Hpg的水平传播和鸡传染性鼻炎的反复发作与饲养密度有很大的关系。最后将分离菌株送匈牙利诗华研发中心进行分型鉴定,确定为A型副鸡嗜血杆菌。根据分离地点将分离菌株命名为A型副鸡嗜血杆菌(山东株)。另外通过与GenBank已发表的Hpg基因序列进行同源性比对分析,结果显示,该基因序列与参考株的基因核苷酸序列同源性达99.0%。 相似文献
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抗副鸡嗜血杆菌血清A和C型株所制备的两个血清型单克隆抗体（MAbs），分别对副鸡嗜血杆菌血清型A、B、C中的各型参考株作HI和dot－blotting试验。一种MAb（E5C12D10）为抗血清型A代表株221，另一种MAb（F2E6）为抗血清型C代表株S1。在两种试验中，不同血清型的MAbs可与对应的血清型中的副鸡嗜血杆菌株血凝（HA）抗原反应，而与血清型B代表株91、147均无反应。故这些MAbs可用于dot－blotting或HI试验进行副鸡嗜血杆菌定型。 相似文献
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鸡支原体,鸡传染性鼻炎双价二联油乳剂灭活疫苗的研究 总被引：4，自引：2，他引：2
应用鸡败血支原体国际标准株（ＭＧＲ）和鸡副嗜血杆菌（ＨＰＧ）国际标准株Ａ型（ＨＰＧ２２１）和Ｃ型（ＨＰＧ６６８）制备了抗鸡支原体病（ＡＭ）和鸡传染性鼻炎（ＩＣ）的双价二联油乳剂灭活疫苗。通过多批次ＳＰＦ鸡免疫试验,进行了疫苗安全性、抗体产生时间及抗体动态变化规律、攻毒保护率、免疫期及现地鸡群的中间试验。结果表明,疫苗接种后１０～１５天可相继产生ＭＧ和ＨＰＧ血清抗体;接种６个月内,强毒攻击的免疫保护率为９１％～１００％;各项试验的指标不低于各自的单苗,具有很好的推广应用价值。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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