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1.
滩羊种质及血液遗传标记研究 总被引：5，自引：0，他引：5
应用聚丙烯酰胺凝胶电泳技术测定我省白银平川区泉乡９４只滩羊的Ｈｂ系统和绵羊一样，在Ｈｂ位点上有两个基因Ｈｂ＾Ａ和Ｈｂ＾Ｂ，具有三种表现型ＨｂＡＡ，ＨｂＢＢＨｂＡＢ，其中等位基因Ｈｂ＾Ｂ和表型ＨｂＢＢ的频率最大，分别为０．８２４５和０．６８０９。 相似文献
2.
撒坝猪血清酯酶多态性与日增重关系的研究 总被引：1，自引：0，他引：1
本研究采用垂直板聚丙烯酰胺凝胶电泳法对１０２头撒坝猪的血清酯酶（Ｅｓ）多态性进行了检测，计算了该位点的基因型频率，基因频率及遗传多样性指数，并用普通方差分析和二因素有互作的最小二乘模型对不同酯酶基因型与几个阶段日增重的关系进行了分析。结果表明，撒坝猪血清酯酶三种基因型ＡＡ、ＡＢ和ＢＢ的频率分别为０．２４５１、０．５９８０和０．１５６９。两个等位基因Ａ和Ｂ的基因频率分别为０．５４４１和０．４５５９。该位点的遗传多样性指数为０．４９６２。在三种基因型中，ＢＢ型个体的２～６月龄平均日增重显著高于其它两种类型，且酯酶基因型与性别之间的互作效应明显，显示了猪的血清酯酶多态性作为２～６月龄平均日增重的遗传标记以进行标记辅助选择的可能性 相似文献
3.
中国矮马血红蛋白多态性的初步研究 总被引：4，自引：0，他引：4
采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。结果表明 :在该位点共发现有 2种基因型 ,由 2个等位基因HbBⅡ 、HbBⅠ 控制。基因型BⅡ /BⅡ、BⅠ /BⅡ的频率分别为 0 62 5 0、 0 375 0 ,其中纯合型BⅡ /BⅡ为优势基因型。基因频率HbBⅡ 、HbBⅠ 分别为 0 81 2 5、0 1 875。其基因杂合度 (H)、个体鉴别概率 (Dp)和亲仔关系排除概率 (PE)分别为 0 30 4 7、 0 4690和 0 1 2 90。 相似文献
4.
三河马血红蛋白的遗传多态性 总被引：2，自引：0，他引：2
采用等电聚焦电泳对25匹三河马进行了血红蛋白多态性的检测，结果表明：该位点共发现有4种基因型，由3个等位基因HbT^BⅡ、HbBIt Hb^AⅡ控制，基因型BⅡ/BⅡ、BI/BⅡ、Bi/BI和AⅡ/BⅠ的频率分别为0.08,0.52,0.36和0.04，其中杂合型BI/BⅡ为优势基因型，等位基因Hb^BⅡ、Hb^BI和HbA^Ⅱ的频率分别为0.34,0.64和0.02。基因杂合度、个体识别概率和亲仔关系排除概率分别为0.47,0.59和0.20。 相似文献
5.
青海半细毛羊血红蛋白多态性的研究 总被引：2，自引：0，他引：2
采用聚内烯酰胺凝胶电泳对海北州海晏县９１只青海半细毛羊的血样进行了血红蛋白多态性的研究。结果表明：海晏县青海半细毛羊的血红蛋白有ＨＢＡＡ、ＨＢＡＢ、ＨＢＢＢ３种基因型．ＨＢ＾Ａ和ＨＢ＾Ｂ等位基因频率分别为０．３７９１和０．６２０９,基因事度为０．４７０８。 相似文献
6.
采用聚丙烯酰胺凝胶电泳地对德令哈地区９５头柴达木黄牛的血红蛋白进行分型。结果发现，柴达木黄牛Ｈｂ的多态性受Ｈｂ和Ｈｂ三个复等位基因控制，构成ＨｂＡＡ、ＨｂＡＢ、ＨｂＡｃ和ＨｂＢＢ四种基因型，各等位基因频率分别为０．８３６８、０．１５７９和０．００５３，基因杂合度为０．２７４８。 相似文献
7.
撒坝猪血清淀粉酶多态性与生长速度关系的研究 总被引：1，自引：0，他引：1
本研究采用垂直板聚丙烯酰胺凝胶电泳法对１１５头撒坝猪血清淀粉酶（Ａｍ）多态性进行了检测,并用二因素有互作的最小二乘模型对不同淀粉酶基因型与生长速度的关系进行了分析。结果表明,所检测到的血清淀粉酶ＡＡ、ＡＢ和ＢＢ３种基因型的频率分别为０．１７３９、０．４２６１和０．４０００,两个等位基因Ａ和Ｂ的频率分别为０．３８７０和０．６１３０,未发现Ｃ基因。该位点的遗传多样性指数为０．４７４４。在３种基因型中,ＢＢ型个体的６月龄体重、２～６月龄日增重和４～６月龄日增重最高,且淀粉酶基因型与性别之间在２０日龄体重、４月龄体重、６月龄体重、０～２０日龄日增重、２～４月龄日增重、２～６月龄日增重和４～６月龄日增重等性状上存在着明显的互作效应,显示出血清淀粉酶多态性作为增重速度的遗传标记以进行标记辅助选择的可能性 相似文献
8.
青海细毛羊血红蛋白和红细胞蛋白质多态性的研究 总被引：5，自引：0，他引：5
采用聚丙烯酰胺凝胶电泳法对４７９只青海细毛羊的血红蛋白和红细胞蛋白质的多态性进行研究。结果发现：①青海细毛羊的血红蛋白基因座上有ＨＢＡＡ，ＨＢＡＢ，ＨＢＡＣ和ＨＢＢＢ四种基因型。ＨＢ＾Ａ，ＨＢ＾Ｃ等位基因频率分别为０．２６４１，０．７３４９和０．００１０；②血红蛋白基因座的基因型频率和基因频率没有性别间差异；③青海细毛羊ＥＰ－１和ＥＰ－２是单态性蛋白质。 相似文献
9.
将８０只３５日龄ＳＰＦ鸡随机分为四组，每组２０只。Ⅰ组为复方中药制剂组，Ⅱ组为中药配方组，Ⅲ组为ＩＢＤ病变组织裂解物组，Ⅳ组为对照组。用药后第１０天对四组鸡同时用ＩＢＤ强毒攻击，各组的保护率为Ⅰ组９５％（１９／２０），Ⅱ组６０％（１２／２０），Ⅲ组７０％（１４／２０），Ⅳ组１５％（３／２０）。Ⅰ组保护率比Ⅱ组和Ⅲ组高２５～３５％（ｐＨ＞０．０１），比Ⅳ组高８０％（Ｐ＜０．０１）。然后选择ＩＢＤ多发地区不同品种的１５～２０日龄雏鸡４４０００只进行临床应用，结果保护率达１００％。说明复方中药制剂对ＩＢＤ有很好的预防效果。 相似文献
10.
夏洛来杂种羊及其亲本的血红蛋白遗传多态性 总被引：2，自引：0，他引：2
采用聚丙烯酰胺凝胶电泳对夏洛来杂种羊及其亲本的血红蛋白多态性进行了遗传检测。结果表明：参试羊表现出ＨＢＡＡ，ＨＢＢＢ和ＨＢＡＢ三种基因型，各群体均以杂合子ＨＢＡＢ为优势基因型，ＨＢ＾Ｂ为优势基因，ＨＢ＾Ｂ频率在０．５１５２￣０．６４２８之间，没有发现ＨＢ＾Ｃ基因；群体基因频率分析和亲子配对资料的遗传分析均证实，绵羊ＨＢ受常染色体位点上两个共显性等位基因的控制，各羊群ＨＢ基因的杂合度在０．５左右。 相似文献
11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
13.
Tropical Animal Health and Production - 相似文献
14.
Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
15.
H Hayashi T Yoshimura J Y Chen 《Comparative immunology, microbiology and infectious diseases》1985,8(2):73-87
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed. 相似文献
16.
Davis MS Willard MD Nelson SL Mandsager RE McKiernan BS Mansell JK Lehenbauer TW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):311-314
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large. 相似文献
17.
B. Cinar Kul N. Bilgen J.A. Lenstra O. Korkmaz Agaoglu B. Akyuz O. Ertugrul 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):449-453
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded. 相似文献
18.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
19.
Ling GV Thurmond MC Choi YK Franti CE Ruby AL Johnson DL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):817-823
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors. 相似文献
20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems. 相似文献