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1.
《中国畜牧兽医》2018,(12)
为探究无菌大鼠深二度烫伤感染模型机体炎性反应,试验利用无菌大鼠分别建立深二度烫伤损伤模型和烫伤后绿脓杆菌感染模型,比较这两种模型和SPF级大鼠深二度烫伤模型在愈合过程、炎性反应及病理变化等方面的异同。20只6周龄雌性Wistar无菌大鼠,在超净工作台内采用恒温恒压烫伤仪94℃作用8s造成大鼠深二度烫伤,随机分为2组,一组伤口感染浓度为1×105 CFU/mL绿脓杆菌菌液0.5mL,一组不做处理,创面保持无菌状态,SPF级Wistar大鼠在同样条件下烫伤,创面不做处理。分别观察3组大鼠的伤口结痂时间、脱痂时间和愈合时间,并在烫伤前(0h),烫伤后24h、3d和7d时,取血清,检测炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素1α(IL-1α)、粒细胞—巨噬细胞刺激因子(GM-CSF)的变化。结果显示,感染绿脓杆菌大鼠组开始结痂所需时间显著长于SPF级大鼠组和无菌大鼠组(P0.05);而脱痂及愈合所需时间显著变短(P0.05);感染绿脓杆菌组大鼠烫伤24h时,皮下组织中可见淋巴细胞,有慢性炎性反应;3d时,真皮深层有化脓性感染,横纹肌周围可见大量炎细胞,烫伤7d时,皮下组织中有轻微血管扩张充血,化脓性感染减轻,无菌烫伤大鼠与SPF级烫伤大鼠皮肤在烫伤后一周内有轻度炎性反应。3组大鼠烫伤后,血清GM-CSF、IL-1α及TNF-α水平均较烫伤前显著升高(P0.05);烫伤24h时,感染绿脓杆菌大鼠组和无菌大鼠组血清IL-1α水平显著高于SPF级大鼠组(P0.05);无菌大鼠组血清GM-CSF水平显著高于SPF级大鼠组和感染绿脓杆菌大鼠组(P0.05);烫伤3d时,染绿脓杆菌大鼠组和无菌大鼠组血清IL-1α水平显著高于SPF大鼠组(P0.05),无菌大鼠组血清GM-CSF水平显著高于染绿脓杆菌大鼠组(P0.05)。综上所述,无菌大鼠对烫伤刺激反应迅速强烈,血清中炎性因子迅速升高,SPF级大鼠深二度烫伤后炎性反应缓慢;无菌大鼠深二度烫伤伤口感染1×105 CFU/mL绿脓杆菌,脱痂时间和愈合时间反而缩短。 相似文献
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为观察壳聚糖接枝庆大霉素缀合物（CS-GT）对葡聚糖硫酸钠（Dextran sulfate sodium salt,DSS）诱导小鼠溃疡性结肠炎的保护效果。30只健康雄性小鼠（C57BL/6J,SPF级）随机平均分为5组，分别为对照组、DSS组和CS-GT低、中、高剂量添加组。处理结束后，观察临床变化、肠道结构、血清生化指标和炎性因子表达。结果发现，与对照组相比，DSS处理组小鼠体重下降（P<0.01），结肠长度缩短（P<0.01），肠道出血及肠绒毛脱落，血清中ALB与TP含量显著降低（P<0.01），促炎因子IL-6表达水平显著升高（P<0.01），抗炎因子IL-4和IL-13表达水平显著降低（P<0.05）。而添加中、高剂量CS-GT组相较于DSS组小鼠体重显著上升（P<0.05）、结肠长度显著增加（P<0.05），肠出血现象减少，血清中ALB与TP含量显著上升（P<0.05），促炎因子IL-6表达水平显著下降（P<0.05），抗炎因子IL-4和IL-13有一定的上升趋势。说明CS-GT对DSS诱导的小鼠结肠炎有明显保护效果，有... 相似文献
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文章旨在研究复方中药提取物对感染大肠杆菌白羽鸡生长性能、血清生化指标及回肠炎性因子mRNA表达的影响，试验将60只60日龄白羽鸡随机分为3组，每组20只（每组分3笼饲养，每笼分别饲养7、7、6只），分别记为健康对照组、感染对照组和复方中药组。除健康对照组外，其余2组每羽腹腔注射2 mL大肠杆菌菌液，观察鸡只反应，待出现明显腹泻症状时开始给药。复方中药组按照100 g/L（以生药量计算）饮水给药，连用3 d，健康对照组和感染对照组不给药。结果显示，感染对照组和复方中药组鸡只接种后均出现腹泻症状，给药第3天，复方中药组粪便分数恢复到与健康对照组相似水平；与感染对照组相比，复方中药组可显著改善鸡只体重下降（P<0.05），显著降低鸡只血清中ALT、AST和LDH活性（P<0.05），显著下调回肠炎性因子IL-1β、IL-6、IL-8和TNF-α基因mRNA表达（P<0.05），显著上调回肠抑炎因子IL-10和TGF-β基因mRNA表达（P<0.05）。综上所述，复方中药提取物能缓解大肠杆菌引起的白羽鸡体重下降和肝肾损伤，并通过抑制肠道促炎因子分泌，以及促进抑炎因子分泌... 相似文献
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为评价中药肠炎宁对猪大肠杆菌感染小鼠肠道的保护效果，并初步研究其机制，本试验将SPF级BALB/c小鼠随机分为空白对照组、大肠杆菌K88组和肠炎宁组3个组，大肠杆菌K88组和肠炎宁组小鼠通过口服产肠毒素性大肠杆菌K88诱导腹泻模型，12 h后分别灌服生理盐水和肠炎宁药液，连续给药3 d,检测不同组别小鼠的存活时间、体重变化、肠道病理变化、结肠长度变化、肠道通透性和炎症因子等指标变化，并分析炎症因子信号通路蛋白表达情况。结果显示，与空白对照组相比，大肠杆菌K88组小鼠出现死亡，存活时间显著缩短(P<0.05),体重显著下降(P<0.05),结肠显著缩短(P<0.05),肠道通透性和血清髓过氧化物酶(MPO)活性显著升高(P<0.05),血清和结肠组织中TNF-α和IL-1β水平显著升高(P<0.05);与大肠杆菌K88组相比，肠炎宁组小鼠存活时间显著延长(P<0.05),体重显著增加(P<0.05),结肠显著增长(P<0.05),肠道通透性和血清MPO活性显著下降(P<0.05),血清和结肠组织中TNF-α和IL-1β水平显著下降(P... 相似文献
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试验旨在研究葡萄籽渣（GSP）替代日粮中玉米对西门塔尔牛生长性能、抗氧化能力、抗炎能力及肠道微生物组成的影响。选取20头18月龄的平均体重为（665.20±66.65）kg的杂交西门塔尔牛，随机分为2组，每组10头，每头为1个重复。对照组（CON组）饲喂基础日粮，试验组（GSP组）使用1.69%的葡萄籽渣替代玉米。预试期7 d，正试期45 d。结果表明：GSP组西门塔尔牛平均日增重、校正后平均日增重分别为0.69、0.75kg/d，均与CON组差异不显著；两组血清抗氧化性能差异不显著；两组炎症因子白介素-1β(IL-1β)含量无显著差异，而GSP组肿瘤坏死因子-α(TNF-α)、IL-6含量低于CON组（P<0.05、P<0.01）;16SrRNA测序结果显示，GSP组肠道微生物组成较CON组具有更高的丰富度及多样性；在门水平上，厚壁菌门是两组中最丰富的细菌，其次是拟杆菌门，GSP组放线菌门的丰度高于CON组（P<0.05）；在属水平上，GSP组Rikenellaceae＿RC9＿gut＿group的丰度有高于CON组的趋势（0.05相似文献
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母猪日粮中添加低聚壳聚糖对哺乳仔猪生长、抗氧化能力和免疫功能的影响 总被引：2，自引：2，他引：0
本试验旨在探讨母猪日粮中添加低聚壳聚糖对哺乳仔猪生长、血液生化指标、抗氧化能力及免疫功能的影响。选用24头胎次、体重和预产期相近的健康大白猪母猪，随机分为3组，每组8个重复，每个重复1头母猪。对照组饲喂基础日粮，Ⅰ、Ⅱ组饲喂分别添加50和100 g/t低聚壳聚糖的基础日粮，试验从母猪妊娠第85天开始至分娩后第21天时结束。结果显示：①Ⅱ组哺乳仔猪腹泻率显著低于对照组和Ⅰ组（P < 0.05），Ⅰ组与对照组差异不显著（P > 0.05）；与对照组相比，Ⅰ、Ⅱ组仔猪21日龄体重显著提高（P < 0.05）。②Ⅰ组仔猪血浆乳酸脱氢酶（LDH）活性和甘油三酯（TG）浓度，Ⅱ组α-淀粉酶（α-AMY）、LDH活性和低密度脂蛋白（LDL）浓度均显著低于对照组（P < 0.05）。③Ⅱ组哺乳仔猪血清超氧化物歧化酶（SOD）活性，皮质醇、白介素-10（IL-10）、白介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）和干扰素-γ（IFN-γ）含量均显著高于对照组和Ⅰ组（P < 0.05），而丙二醛（MDA）浓度显著低于对照组（P < 0.05）。④低聚壳聚糖组母猪初乳中免疫球蛋白G（IgG）、IgA和IgM含量均显著高于对照组（P < 0.05）；Ⅰ组仔猪血清IgG和IgA含量、Ⅱ组仔猪血清IgA含量均显著高于对照组（P < 0.05）。综上所述，母猪日粮中添加一定量的低聚壳聚糖能够提高哺乳仔猪的抗氧化能力、增强机体免疫能力、降低腹泻率，从而提高哺乳仔猪生长性能。 相似文献
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试验旨在研究甜叶菊异绿原酸盐对产蛋初期蛋鸡产蛋性能、免疫指标及脂质代谢指标的影响。选取健康且生产性能相近的20周龄海兰灰蛋鸡540只，随机分为3组，每组设置6个重复，每个重复30只，对照组饲喂基础饲料，试验组分别在基础饲粮中添加100 mg/kg（低剂量组）、200 mg/kg（高剂量组）甜叶菊异绿原酸盐，预试期7 d，正试期28 d。结果表明：(1)与对照组相比，低、高剂量组对产蛋率、平均蛋重、料蛋比均无显著性影响（P>0.05）；但均能显著降低劣蛋率（P<0.05）。(2)与对照组相比，低、高剂量组均可显著降低蛋鸡血清中肿瘤坏死因子α(TNF-α)、γ干扰素（IFN-γ）、白介素-6(IL-6)、白介素-8(IL-8)含量（P<0.05），低、高剂量组可显著提高血清白介素-10(IL-10)、免疫球蛋白M(IgM)含量（P<0.05），高剂量组能够显著提高血清免疫球蛋白G(IgG)含量（P<0.05）。(3)与对照组相比，低、高剂量组均可以显著降低TC和LDL-C含量（P<0.05），并显著提高HDL-C含量（P<0.05），但对TG含量无... 相似文献
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试验旨在通过脂多糖（lipopolysaccharide,LPS）诱导奶牛子宫内膜上皮细胞构建子宫内膜炎体外感染模型,研究子宫内膜炎对奶牛子宫容受性因子大分子转膜黏蛋白-糖蛋白1（MUC-1）、高度保守的分泌型WNT家族亚型糖蛋白（Wnt-7a）、β受体1（IFNAR1）、IFNAR2、Integrin αvβ3 mRNA及蛋白相对表达量的影响,进而阐明子宫内膜炎引起奶牛屡配不孕和繁殖率低下的机制。采用组织块法分离培养奶牛子宫内膜上皮细胞,免疫荧光方法鉴定细胞纯度,不同浓度LPS刺激子宫内膜上皮细胞,在不同时间点用CCK8测定细胞存活率,ELISA检测炎性因子白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、IL-6、IL-8的分泌变化,实时荧光定量PCR和Western blotting分别检测子宫内膜感染模型中子宫容受性因子MUC-1、Wnt-7a、IFNAR1、IFNAR2、Integrin αvβ3 mRNA及蛋白的表达变化。结果显示,通过组织块法纯化获得的奶牛子宫上皮细胞数量较多,经免疫荧光角蛋白染色证实纯度较高。采用CCK8测定细胞存活率发现,与对照组相比,浓度为0、5、10、50μg/mL的LPS作用6、12、24、48 h后,细胞活力无显著变化（P>0.05）;浓度为100μg/mL的LPS作用24 h时,细胞存活率显著低于对照组（P<0.05）,细胞培养上清中的IL-1β、TNF-α、IL-6、IL-8含量均显著高于对照组及低浓度组（P<0.05）,引起细胞的炎症反应。与对照组相比,模型组MUC-1 mRNA及蛋白的相对表达量均极显著增加（P<0.01）;Wnt-7a mRNA及蛋白的相对表达量均极显著下降（P<0.01）;Integrin αvβ3、IFNAR1和IFNAR2 mRNA相对表达量均极显著下降（P<0.01）,蛋白相对表达量均显著下降（P<0.05）。结果表明,浓度为100μg/mL的LPS作用24 h可成功构建子宫内膜炎体外感染模型,子宫内膜炎对奶牛子宫容受性因子MUC-1、Wnt-7a、IFNAR1、IFNAR2、Integrin αvβ3 mRNA及蛋白表达的影响可能是引起奶牛屡配不孕和繁殖率低下的重要原因。 相似文献
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试验旨在观察丁酸梭菌-龙眼多糖发酵液预防给药对溃疡性结肠炎（ulcerative colitis，UC）模型小鼠的防治作用。30只SPF级雄性BALB/c小鼠随机分成空白组、模型组和发酵液组，10只/组。按10 mL/kg体重的剂量标准，空白组和模型组灌胃无菌去离子水，发酵液组灌胃丁酸梭菌-龙眼多糖发酵液，灌胃持续7 d。第8天开始，模型组和发酵液组连续7 d自由饮用5%葡聚糖硫酸钠（dextran sulfate sodium，DSS）溶液诱导UC模型，空白组自由饮用无菌水。试验第14天，称量所有小鼠体重，进行疾病活动指数（disease activity index，DAL）评分、临床评分，摘眼球采血后处死并解剖小鼠，取结肠肠段测量长度并进行组织学损伤评分，同时用甲醛固定后制作切片。ELISA检测血清中白细胞介素-6（interleukin-6，IL-6）、IL-10及肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）含量。结果表明，UC模型造模成功，模型组发病症状明显。与模型组相比，发酵液组小鼠体重下降程度显著降低（P<0.05），DAL评分、临床评分、结肠缩短程度及组织学损伤评分均极显著下降（P<0.01）。发酵液组小鼠结肠组织表现出相对完整的上皮层结构及隐窝，仅有部分炎症细胞侵入上皮组织。ELISA检测结果显示，发酵液组IL-6和TNF-α含量显著低于模型组（P<0.05），IL-10的含量显著高于模型组（P<0.05）。说明丁酸梭菌-龙眼多糖发酵液对DSS诱导的小鼠溃疡性结肠炎具有一定的防治作用，其作用机制可能与降低促炎因子IL-6和TNF-α含量、提升抗炎因子IL-10含量抑制炎症有关。 相似文献
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试验旨在探讨在饲粮中添加不同比例的辣木叶多糖对三黄鸡生长性能、血清生化指标和免疫功能的影响。选取1日龄三黄鸡600羽,随机分成5组,每组8个重复,每个重复15羽三黄鸡。对照组（CON组）饲喂基础饲粮,T1组、T2组、T3组、T4组分别在基础饲粮添加0.08%、0.12%、0.16%、0.20%的辣木叶多糖。试验期42 d。结果显示,与对照组相比,1～21日龄,T1组、T2组、T3组三黄鸡的料重比显著降低（P<0.05）,T3组42日龄三黄鸡的体重显著升高（P<0.05）;1～42日龄,各试验组三黄鸡的料重比均显著降低（P<0.05）。与对照组相比,T2组血清白蛋白含量显著升高（P<0.05）,T3组血清尿素氮含量显著降低（P<0.05）;T1组、T2组、T3组血清葡萄糖含量显著增加（P<0.05）;T3组胸腺指数显著升高（P<0.05）,T1组、T3组、T4组血清白细胞介素-4 (IL-4)含量显著升高（P<0.05）,T2组、T3组血清白细胞介素-6 (IL-6)含量显著降低（P<0.05）,各试验组血清白细胞介素-1β (IL-... 相似文献
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研究１日龄ＳＰＦ雏鸡感染网状内皮组织增殖病病毒后局部免疫组织均浆涂片的免疫学变化。结果表明：雏鸡感染ＲＥＶ后其局部免疫组织中,淋巴细胞数、ＡＮＡＥ、Ｔ细胞数以及颗粒型和弥散型ＡＮＡＥ＾＋Ｔ淋巴细胞数明显低于未感染雏鸡。说明病消化道和呼吸道相关的局部免疫组织的细胞免疫呈现抑制。 相似文献
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Oryan A Khalafi-Nezhad A Toloo N Soltani Rad MN 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(10):585-591
In this investigation, the effects of synthesized 4-chloro-2,6-bis-(2-hydroxyl-benzyl)-phenol (CBHBP) on cutaneous wound healing and growth of some of the wound contaminating microorganisms were studied. The antibacterial effects of this compound were then evaluated on Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Klebsiella spp., using solid dilution method. It was demonstrated that CBHBP has a significant antimicrobial activity against S. aureus but it is not effective in the case of other microorganisms studied in this experiment. The effect of local administration of CBHBP on healing of a standard full-thickness 2 cm skin incision of skeletally mature rats was evaluated. Histological changes together with mechanical properties and dry weight content of the healing tissues at the site of the lesions were assessed in treated and untreated animals. It was observed that the injured area of the treated animals was more organized and showed more fibroblasts and less inflammatory cells. Much better maturation criteria in treated tissues were observed in comparison with those of the untreated ones which contained numerous polymorphonuclear inflammatory cells after 14 days post-injury. Many infiltrated macrophages and lymphocytes were present even 28 days after injury induction in the haphazardly organized dermis and also in subcutaneous tissues of the untreated animals. The percentage dry weight content of the treated lesions at 14 days post-injury was remarkably higher than those of the untreated animals. The results of biomechanical tensile testing showed that the ultimate tensile strength and stress of the injured skin of the treated animals were higher than those of the untreated ones. From these results, it could be concluded that CBHBP can be effective on wound healing and may be considered as a treatment regimen after evaluating its mechanism of action as well as testing its contraindications. 相似文献
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McKay L Rose CD Matousek JL Schmeitzel LS Gibson NM Gaskin JM 《Journal of the American Animal Hospital Association》2007,43(6):307-312
A total of 100 Pseudomonas aeruginosa (P. aeruginosa) isolates were collected over a 1.5-year period from cases of canine otitis. Sensitivities to enrofloxacin, marbofloxacin, and orbifloxacin were determined using minimum inhibitory concentration testing (MICT). Isolates were also tested for sensitivities to enrofloxacin and marbofloxacin using disk-diffusion susceptibility testing (DDST). Isolates were significantly more sensitive to marbofloxacin than to enrofloxacin (z = -4.57; P<0.05) or orbifloxacin (z = -5.02; P<0.05). Agreement was 87% between MICT and DDST for marbofloxacin, with approximately equal numbers of overestimation and underestimation errors. Agreement was 74% between MICT and DDST for enrofloxacin, but DDST tended to overestimate the number of enrofloxacin-susceptible strains. These results suggest that marbofloxacin is more effective against P. aeruginosa than either enrofloxacin or orbifloxacin and that relying on DDST may lead to ineffective enrofloxacin treatment. 相似文献
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LI Hai-hua ZHU Qi WANG Shi-qiong YANG Chun-lei ZHAO Xiang-hua QIAO Jia-yun WANG Wen-jie 《中国畜牧兽医》2017,44(1):262-267
To investigate the molecular mechanism of the inflammatory response in the piglets infected with enterotoxigenic E. coli (ETEC) K88, piglets were infected with ETEC K88,the IL-8 content in serum of piglets were assayed by ELISA,and the mRNA relative expression levels of TLR2/4 and its signal transduction pathway related genes (MyD88,Tollip and Bcl3) in mesenteric lymph nodes were detected by quantitative Real-time PCR. The results showed that compared with control group,the content of IL-8 in serum and the expressions of TLR2/4 in lymph nodes were all extremely significantly or significantly increased at 6 and 24 h after infection (P<0.01;P<0.05),and the IL-8 content and TLR2/4 mRNA expression at 24 h after infection were all significantly lower than those at 6 h after infection (P<0.05).In addition,the expressions of MyD88,Tollip and Bcl3 in lymph nodes were all extremely significantly increased at 24 h after infection compared with control group (P<0.01), but there was no significant difference between experimental group and control group at 6 h after infection (P>0.05). In conclusion,ETEC K88 infected piglets might produce inflammatory cytokines IL-8 through the TLR2/4-MyD88 signaling pathway,which could promote the inflammatory reaction in piglets. This inflammatory response might be regulated by Tollip and Bcl3,which could weak the inflammatory intensity in piglets. 相似文献
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试验探究了短期灌喂民猪源贝莱斯芽孢杆菌M2对SD大鼠生长、血液生化与免疫、肠道菌群等相关指标的影响，拟为贝莱斯芽孢杆菌在畜禽中的应用提供参考依据。选取21日龄、体重相近、SPF级健康SD雌性大鼠共24只，将其随机分成2组，每组6个重复，每个重复2只。预饲期3 d。试验期，对照组灌喂生理盐水0.2 mL/d，试验组灌喂猪源贝莱斯芽孢杆菌M2菌液0.2 mL/d；每天灌喂1次，连续灌喂7 d。35和45日龄，每个重复随机选取1只大鼠处死采样。结果表明，短期灌喂贝莱斯芽孢杆菌M2有增加末重和日增重、降低日采食量和料重比的趋势（P>0.05）。短期灌喂贝莱斯芽孢杆菌M2显著降低了35日龄大鼠血浆中甘油三酯浓度和IgA、TNF-α的水平（P<0.05），以及45日龄大鼠血浆中IL-6的浓度（P<0.05），并显著提高35日龄大鼠血浆中IgM的浓度（P<0.05）；对35日龄大鼠肠道菌群无显著影响（P>0.05），可显著降低45日龄大鼠放线菌门和变形菌门的比例（P<0.05）。综上所述，短期灌喂贝莱斯芽孢杆菌M2可以调控SD大鼠的脂代谢、免疫力和肠道多种菌群的丰度。 相似文献
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ZHAO Zeng-cheng LIN Shu-qian HUANG Zhong-li LI Gui-ming YANG Shi-fa FENG Min-yan SONG Min-xun FU Jian LI Ying SHANG Jing 《中国畜牧兽医》2017,44(1):282-288
In order to verify the effect of Baihu Dingchuan oral liquid of new veterinary drugs,the effects of infectious bronchitis virus (IBV) on the proliferation inhibition test, tracheal cilia movement test and SPF chickens infected with IBV were tested.The test solution was mixed with an equal amount of IBV solution,inoculation of 11-day-old SPF chicken embryo,incubation at 37℃ for 168 h.RT-PCR was performed to detect IBV in kidney of live chick embryo.The results showed that the survival rate of 168 h chicken embryo was 80% in the drug group,but the positive control group was 20%, and there was significant difference between two groups (P<0.05). In the test drug group, no band was detected by RT-PCR, but the target band appeared in the positive control group.After 40 days old SPF chickens were administered continuously for 3 days, 0.02 mL of ink was injected into the trachea. The distance of ink movement in 1 min was measured. The results showed that the migration distance of ink was extremely significantly higher than that of saline control group (P<0.01).In the 17-day-old SPF chickens, IBV-M41 was infused into the nasal mucosa with 0.3 mL. After 24 h, 1 mL/kg was administered for 5 days. The results showed that there were significant differences in clinical symptom score and cure rate between the test drug group and the control group (P<0.05). There was no significant pathological changes in the tracheal mucosa of the experimental group, but the pathological changes in the positive control group were significant. The above results showed that Baihu Dingchuan oral liquid had good inhibitory effect on IBV in vitro, which could promote the movement of tracheal cilia, and had good clinical control effect on infectious bronchitis. 相似文献
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Westgate SJ Percival SL Knottenbelt DC Clegg PD Cochrane CA 《Veterinary microbiology》2011,150(1-2):152-159
Horse wounds have a high risk of becoming infected due to their environment. Infected wounds harbour diverse populations of microorganisms, however in some cases these microorganisms can be difficult to identify and fail to respond to antibiotic treatment, resulting in chronic non-healing wounds. In human wounds this has been attributed to the ability of bacteria to survive in a biofilm phenotypic state. Biofilms are known to delay wound healing, principally due to their recalcitrance towards antimicrobial therapies and components of the innate immune response. This study describes the presence of bacterial biofilms within equine wounds. Thirteen 8-mm diameter tissue samples were collected from (n=18) chronic wounds. Following histological staining, samples were observed for evidence of biofilms. Fifty one wounds and control skin sites were sampled using sterile swabs. Control skin sites were on the uninjured side of the horse at the same anatomical location as the wound. The isolated bacteria were cultured aerobically and anaerobically. The biofilm forming potential of all the isolated bacteria was determined using a standard crystal violet microtitre plate assay. Stained tissue samples provided evidence of biofilms within 61.5% (8 out of 13) equine wounds. In total 340 bacterial isolates were identified from all the equine wound and skin samples. Pseudomonas aeruginosa and Enterococcus faecium were the most predominantly isolated bacterial species from equine wound and skin samples respectively. Staphylococcus was the most commonly isolated genus in both environments. Bacteria cultured from chronic and acute wounds showed significantly (P<0.05) higher biofilm forming potential than bacteria isolated from skin. This paper highlights preliminary evidence supporting the presence of biofilms and a high microbial diversity in equine chronic wounds. The presence of biofilms in equine wounds partly explains the reluctance of many lower limb wounds to heal. Non-healing limb wounds in horses are a well documented welfare and economic concern. This knowledge can be used to shape future treatments in order to increase the healing rate and decrease the costs and suffering associate with equine wounds. 相似文献