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1.
青龙湖冬季浮游植物群落结构及其与环境因子的关系 总被引：1，自引：0，他引：1
为探究青龙湖浮游植物群落结构特征及其水环境影响因子,确定水体营养化现状,于2016年12(冬季)对青龙湖10个采样点浮游植物种类组成、丰度与生物量以及水质理化指标进行调查分析。结果显示,冬季青龙湖共鉴定出浮游植物5门、17属、50种(含变种),硅藻和绿藻是青龙湖浮游植物优势类群,浮游植物种类组成表现为硅藻-绿藻型;优势类群为硅藻门颗粒直链藻(Melosira granulata)、颗粒直链藻极狭变种(M.granulata var.angustissima)、颗粒直链藻极狭变种螺旋变型(M.granulata var.angustissima f.spiralis)及蓝藻门类颤鱼腥藻(Anabaena oscillarioides)、水华束丝藻(Aphanizomenon flos-aquae),优势种在各采样点分布差异不明显。冬季浮游植物丰度均值为6.71×104个/L,生物量均值为0.07mg/L;各采样点Margalef指数、Shannon-Wiener指数与Pielou指数分别为0.98~2.31、0.61~2.37及0.19~0.84。冗余分析(RDA)表明,pH、溶解氧、溶解氧饱和度、水温与氧化还原电位与青龙湖浮游植物群落结构关系最为密切。Margalef丰富度指数、Shannon-Wiener多样性指数与Pielou均匀度指数评价水质结果为中度污型水平。 相似文献
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里下河腹地位于江淮两大水系交融地带,人类活动严重影响区域水生态环境。鉴于浮游植物群落结构及多样性可作为水环境变化重要指示生物,于2015-2017年对里下河腹地典型湖泊大纵湖浮游植物群落结构及环境因子进行逐月采样调查,分别采用藻类生物学指标和综合营养指数对大纵湖营养状态进行综合评价,并且基于相关性分析法,探讨浮游植物与水环境因子的响应关系。结果显示,大纵湖在调查期间共鉴定出浮游植物7门、123种,以绿藻、硅藻、蓝藻为主,分别为50、35、18种,占总种数的40.65%、28.46%、14.63%。全湖各采样点浮游植物的平均丰度为3.70×106 ~6.25×106 个/L;空间上略有差异,夏季藻类丰度较高,冬季最低。大纵湖各个采样点浮游植物优势种基本相同,共8种。浮游植物物种多样性指数和均匀度年均值分别为2.74和0.78,表明大纵湖处于轻-中度污染状态。综合营养指数显示,大纵湖处于轻度富营养状态,两者结果基本一致。相关性分析表明,水温（T）、透明度（SD）、浊度（TUB）和总氮（TN）是影响大纵湖浮游植物群落结构的主要水质指标。 相似文献
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为了给巢湖水质管理及富营养化防治提供科学依据，根据水系特征，在巢湖东、西湖区湖心、裕溪河出湖口、丰乐河、派河和南淝河共设置8个采样点，2013年12月至2014年11月共采样12次，分别代表冬季(12月-次年2月)、春季(3-5月)、夏季(6-8月)和秋季(9-11月)，对巢湖水体理化因子和后生浮游动物（枝角类、桡足类、轮虫）群落进行逐月周年调查。基于叶绿素(Chl-a)、总磷(TP)、总氮(TN)、化学需氧量(CODMn)、透明度(SD)等水体理化指标，运用综合营养状态指数(TLI)和生物多样性指数，对不同湖区受污染程度和营养状态进行综合评价。结果显示，巢湖共检出后生浮游动物42种（属），优势种矩形龟甲轮虫(Keratella quadrata)、萼花臂尾轮虫(Brachionus calyciflorus)为富营养化水体或有机质较多水体的常见种，不同季节的优势种存在较大差异；总体上，后生浮游动物全年平均丰度371.6 个/L（191.5~600.0 个/L）和全年平均生物量3.0 mg/L（2.0~4.6 mg/L）均呈现西半湖高于东半湖、夏秋季高于冬春季的分布特点；其群落结构也存在显著的季节差异和空间差异，矩形龟甲轮虫、萼花臂尾轮虫等是造成差异的主要种类。水温(WT)和Chl-a是影响枝角类丰度的重要因子，桡足类丰度与WT呈显著正相关，而轮虫丰度受TLI(∑)指数的显著影响。TLI(∑)指数均值为60.33（52.18~66.28），指示巢湖总体处于轻度至中度富营养化状态。Shannon-Wiener多样性指数和Margalef丰富度指数表明，巢湖总体处于中度污染状态，不同湖区水体具有明显的不均衡性。 相似文献
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研究金华地区不同水系浮游植物群落结构特征及其对水质的指示作用,为金华江流域的水生态保护与管理提供科学依据。2018年6月（夏季）、9月（秋季）和2019年1月（冬季）、4月（春季）,对调查水域20个采样点的浮游植物进行调查分析。计算浮游植物群落多样性指数,进行聚类与非线性多维标序（NMDS）排序,并基于浮游植物细胞密度、Pielou均匀度指数以及Shannon-Wiener多样性指数对金华地区不同水系的水质进行评价。结果表明：（1）浮游植物调查有7门184种,其中硅藻（74种）和绿藻（63种）为主要成分,分别占总物种数的40.22%和34.24%,表现出硅藻+绿藻类型;时空差异明显,秋季种类较为丰富,各采样点种类丰富度不一;优势种组成存在季节性差异变化,以硅藻门居多,其中常见优势种有脆杆藻（Fragilaria sp.）、颗粒直链藻（Melosira granulata）、卵形隐藻（Cryptomonas ovata）和小球藻（Chlorella sp.）;（2）浮游植物的年均细胞密度和年均生物量分别为(19.37±12.70)×104 个/L和(0.30±0.19) mg/L;（3）浮游植物群落的Margalef丰富度指数、Pielou均匀度指数、Shannon-Wiener多样性指数和Simpson多样性指数的全年变化范围分别为2.28~5.54、0.71~0.89、2.38~3.69和0.78~0.97。金华地区不同水系浮游植物群落受轻度干扰且处于稳定状态,且在时间变化上无显著差异（P＞0.05）;水质清洁至中度污染状态,水生态状况整体良好。 相似文献
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金海水库浮游植物调查及群落结构研究 总被引：2，自引：0，他引：2
为了了解和掌握金海水库水质状况,于2012年6月至2013年5月对金海水库水体理化指标及浮游植物的群落结构进行了逐月监测。采样期间共鉴定出浮游植物7门33属45种,其中绿藻门最多,15属24种;硅藻门次之,8属11种;蓝藻门、裸藻门、甲藻门、隐藻门和金藻门分别鉴定出4属4种、2属2种、2属2种、1属1种、1属1种。金海水库浮游植物数量变化范围20.1×104~335×104个/L,夏季最多,春季最少。浮游植物数量与环境因子相关性分析表明,水体中浮游植物数量主要受水温以及总磷浓度影响。优势度分析表明,金海水库浮游植物优势种有小球藻、肥壮蹄形藻、中型脆杆藻、微小色球藻等31种。金海水库浮游植物多样性指数和均匀度指数分别变化在1.29~3.52和0.38~0.82。金海水库水体浮游植物群落结构较为稳定,整体上以绿藻-硅藻型为主。金海水库水质总体为轻度污染。 相似文献
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大清河水系保定段城市河道浮游植物群落结构及水质评价 总被引：4，自引：0，他引：4
分别于2012年5、7、9、11月对大清河水系保定段城市河道的7个样点进行4次采样,调查浮游植物的群落结构并对水质进行评价。结果表明,在保定段城市河道中共鉴定出浮游植物7门、140种(属);其中,绿藻门69种,蓝藻门15种,硅藻门3种,裸藻门4种,隐藻门21种,金藻门24种,甲藻门4种。浮游植物种类数量变化规律为:5月>7月>9月>11月,位于上游清水桥采样点的浮游植物多样性均大于其他采样点。各点浮游植物密度均超过1000个/mL,水体达到富营养化程度。利用理化指标进行水质评价,大清河水系在流入保定市区之前水体处于中营养状态,流出保定市区时水体处于重度富营养状态。浮游植物密度指数评价结果与理化指标评价结果基本一致,而Shannon-Wiener物种多样性指数和Pielou均匀度指数的评价结果与理化指标评价结果存在明显的差异。讨论了生物指数法对于城市河道水质评价的适用性。 相似文献
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在京杭运河苏北段秋冬两季开展水环境监测和浮游植物调查,为京杭运河苏北段的水生态环境保护提供参考。2020 年 10 月（秋季）和 2021 年 1 月（冬季）,对京杭运河苏北段布设的 16 个采样点开展了系统的水环境和浮游植物调查,根据Shannon-Wiener 多样性指数、 Pielou 均匀度指数和浮游植物细胞丰度进行了水质及水体营养状态的评价,利用 Pearson 相关性分析明确了浮游植物与环境因子的关系。结果显示：京杭运河苏北段秋冬季共采集到浮游植物 8 门 101 种,其中秋季 7 门 52 种、冬季 5 门 63 种,秋冬季均是以绿藻门和硅藻门为主,2个门类的种类数在秋冬季浮游植物种类总数的占比分别达到了 73% 和 82%;秋季浮游植物细胞丰度均值为 49.1×104 个/L,生物量均值为 0.344 mg/L,冬季浮游植物细胞丰度均值为 15.0×104 个/L,生物量均值为 0.193 mg/L;秋冬季浮游植物优势种主要以硅藻门（Bacillariophyta）和绿藻门（Chlorophyta）为主;秋季浮游植物的多样性评价结果优于冬季,秋冬季水体营养状态均为中营养型,相关性分析表明氮对京杭运河苏北段浮游植物群落结构的影响大于磷;另外,行船引起的水体扰动也是造成浮游植物群落差异的重要因素。 相似文献
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浮游动物群落结构是定量描述河道水生态系统功能的重要指标,动物种类及现存量变化能灵敏地反映水域生态环境的动态变化。为探究涡河中下游浮游动物群落结构及其影响因素,于2016年12月至2017年9月分季度监测了其群落组成和水质变化,根据涡河中下游水闸的位置,将整个涡河划分为5个河段,每个采样河段均设置2个采样点,共计10个样点;采用浮游动物种类、密度、生物量和多样性指数,对涡河水体的营养状态和污染情况进行了评价,通过群落指标与水质指标的冗余度,识别群落结构的影响因子。结果表明:(1)涡河中下游4次采样共检测出浮游动物56种,其中原生动物13种,轮虫20种,枝角类13种,桡足类10种;(2)4个季度的生物量为0.12～8.87 mg/L,均值为1.78 mg/L;Shannon-Wiener多样性指数为0.22～3.17,均值为2.13;水体整体处于中度富营养状态,污染指数为中污型;(3)春季的浮游动物优势种数较其他三季高,春季多样性指数较夏冬略高,明显高于秋季;(4)义门镇采样点秋季多样性指数接近于严重污染状态,城西镇、高炉镇采样点秋季多样性指数均处于严重污染状态,广布多肢轮虫为3个采样点的绝对优势种,在水质治理中应引起重视;(5)冗余度分析表明,环境因子对浮游动物时空分布的总体变异解释度为60.70%,流速、水温和溶解氧是影响涡河中下游浮游动物群落结构的主要环境因子。 相似文献
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为了研究三峡大坝修建后库区生态环境的变化,探究大坝修建对库区底泥细菌群落结构和多样性的影响,2013年夏季在香溪河回水区(河口、中游)与河流区(上游)依次选取6个采样点,通过构建16S r DNA基因克隆文库,分析底栖微生物群落结构和多样性的变化规律。结果表明,香溪河调查水域共检出细菌类群15门、87属。在门水平上,回水区和河流区的优势菌门均为变形菌门;在属水平上,回水区和河流区的优势菌属存在显著差异(P0.05),很可能是由于受长江回水和支流高岚河影响所致。芽孢杆菌属所占的比例,从回水区的河口处至中游处逐渐减少至河流区消失,结合芽孢杆菌属易生活在底泥深处的特性,很可能由于大坝的修建,香溪河的上游(即河流区)底泥冲刷至下游(即回水区)河段,被三峡大坝拦截而沉积下来,导致越靠近大坝处,芽孢杆菌属的含量越高。在底泥细菌群落多样性中,Simpson、Shannon和Margalef多样性指数空间分布总体上呈"中间高、两头低"的规律;3种多样性指数最高的采样点均出现在回水区,且回水区的多样性指数均高于河流区,很可能是因其还与旧州河、卜庄河以及周溪河这些支流的交汇,导致河流区底泥细菌群落多样性不稳定。三峡大坝对香溪河底栖微生物的群落结构和多样性产生了显著影响,而大坝修建导致库区回水区水深增加、流速降低、底层水温变化、泥沙沉积增加等因素是造成这种差异的可能原因。 相似文献
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2015年4月在三峡水库库首、库中、库尾分别选择香溪河、澎溪河、御临河进行采样,比较浮游植物群落特征和浮游植物功能类群的异同,并用综合营养指数对水质进行评价。结果显示,3条支流浮游植物群落特征各有不同。3条支流共鉴定出浮游植物8门、46属、68种,其中,御临河口（50种）＞澎溪河口（33种）＞香溪河口（20种）,主要为硅藻和绿藻。御临河、澎溪河、香溪河的浮游植物平均密度分别为19.6×104 个/L、1.18×104个/L、0.9×104个/L,平均生物量分别为0.64 mg/L、0.07 mg/L、0.035 mg/L,御临河现存量明显高于其它2条支流。3条支流共得到23个功能群,优势功能类群为御临河（G, C, P）,澎溪河（LM, MP）,香溪河（F, Y）。研究表明,库尾、库中和库首河口浮游植物种群结构组成相似,从库尾支流到库首支流浮游植物种类和现存量逐渐减少。浮游植物库尾支流由硅藻为优势种的功能群转变为库中甲藻为优势种和库首绿藻为优势种的功能群。浮游植物现存量从库首到库尾支流逐渐增加,对透明度的变化有所影响;而库首到库尾支流水体氮、磷营养更多地取决于蓄水产生的水文水动力变化。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
15.
Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
16.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
17.
Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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19.
Qin-Cheng Huang Di‐Gen Qin Bei‐Ping Tan Tao Du Yuan-Zhi Yang Qi-hui Yang Shu‐Yan Chi Xiao-hui Dong 《Aquaculture Research》2020,51(2):816-827
A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg. 相似文献
20.
William King V David L. Berlinsky Craig V. Sullivan 《Fish physiology and biochemistry》1995,14(6):489-500
Plasma estradiol-17 (E2), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E2 and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E2 and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass. 相似文献