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试验从吉林省通化某地发病死亡鸭子病料中分离到1株具有血凝性的病毒,通过血凝试验、血凝抑制试验、血清中和试验及融合蛋白(F)基因的扩增测序,初步鉴定为新城疫病毒,命名为TH-1株。该病毒鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)及6周龄鸡静脉接种致病指数(IVPI)分别为53.4 h、1.85和2.575,表明该新城疫病毒为强毒。F蛋白多肽裂解位点为112-RRQKRF-117,符合新城疫强毒株裂解位点氨基酸序列,进一步证明该毒株为新城疫强毒株。F基因核苷酸及氨基酸同源性比对发现,TH-1株与中国野鸭源新城疫病毒分离株mallard China HLJ株的同源性最高,核苷酸同源性达到了99.3%,同为新城疫基因Ⅶ型毒株,与目前新城疫流行的主要基因型Ⅶ型相一致,为鸭源新城疫的有效防制提供了理论基础。 相似文献
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鸵鸟新城疫病毒野毒株的分离及其生物学特性 总被引：3，自引：0，他引：3
用鸡胚从疑似鸵鸟新城疫的送检病料中分离到 2株病毒。 2株病毒均能凝集鸡红细胞 ,且能被抗新城疫病毒 ( NDV)阳性血清抑制 ;用抗 NDV单抗 PEG夹心 ELISA测定 2株分离毒均为阳性。对其中 1株作进一步生物学特性鉴定 ,按照国际上规定的 NDV毒力判定标准测定的该毒株最低致死量致死鸡胚的平均死亡时间 ( MDT)为 54h,1日龄雏鸡脑内接种致病指数 ( ICPI)为 1 .88,6周龄雏鸡静脉接种致病指数 ( IVPI)为2 .75。结果表明 ,本分离株为鸵鸟新城疫病毒强毒 相似文献
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为了查明某大雁养殖场大雁发病原因,应用血凝和血凝抑制试验对病原进行了分离鉴定,同时对其致病性进行了测定.结果表明,该分离病毒为新城疫病毒(命名为DQBT01),该病毒能使鸡红细胞发生凝集,且这种凝集能被鸡新城疫标准阳性血清所抑制,同时其F基因检测阳性.经测定,其鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种的致病指数(ICPI)和6周龄雏鸡静脉接种致病指数(IVPI)分别为55 h、1.68、2.54,表明该新城疫病毒为强毒.本次病毒分离表明,在人工饲养条件下,野生水禽可以感染新城疫病毒并导致发生疾病,因此提示有必要对此病毒做进一步研究. 相似文献
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从梁山县1例以肠道糠麸样溃疡,胰腺、脾脏肿胀且表面有大小不等的灰白色坏死灶为主要特征的病死鹅中分离到1株病毒。血凝试验和血凝抑制试验表明该病毒分离株具有血凝性,且其血凝特性能被新城疫标准阳性血清抑制,而不被H9N2亚型禽流感病毒标准阳性血清所抑制。毒力测定结果表明该病毒鸡肧平均致死亡时间(MDT)为49.7h;1日龄SPF鸡脑内接种分离病毒致病指数为1.76;6周龄SPF鸡静脉接种致病指数(IVPI)为2.78。 相似文献
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1997年笔者在江苏省发现一种新的鹅病毒性传染病——鹅副黏病毒病。鹅副黏病毒为禽副黏病毒Ⅰ型,根据国际上规定三项判定新城疫毒力的标准,即最小致死量致死鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)和6周龄非免疫雏鸡静脉接种致病指数(IVPI)等加以评价,鹅副黏病毒分离株对鸡均具有与新城疫病毒速发型相似的毒力,属于新城疫强毒力毒株。用鹅胚和雏鹅参照鸡的标准,即最小致死量致死12日龄鹅胚平均死亡时间、1日龄雏鹅脑内接种致病指数和6周龄非免疫雏鹅静脉接种致病指数等加以评价,鹅副黏病毒分离株对鹅均具有与新城疫病… 相似文献
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分离自鹌鹑自然流行新城疫病例的两株新城疫病毒(NDV),对6周龄鸡静脉接种致病指数(IVPI)、一日龄鸡脑内接种致病指数(ICPI)、最低致死剂量致死鸡胚的平均对间(MDT)、人 O 型及鸡、绵羊、黄牛、马、长白猪、家兔、豚鼠、鹌鹑等不同动物红细胞的血凝活性、血凝解脱及血凝素热稳定性和鹌鹑的致病力进行了检查.同时与分离于自然新城疫病死鸡的2株病毒及参照检验用北京强毒株(E_(48)E_8)做对比检验。结果,所测指标在5株病毒间几无差异. 相似文献
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5株禽新城疫病毒新疆分离株F基因序列测定与遗传进化分析 总被引：1，自引：1，他引：0
从新疆乌鲁木齐市郊区养禽场发病禽群中分离到4株鸡源新城疫病毒（Newcastle disease virus,NDV）和1株鸽源 NDV。经血凝HA试验鉴定,NDV分离株均具有血凝性,5个毒株的血凝特性均可被NDV阳性血清所抑制,而不能被禽流感病毒(H5亚型与H9亚型)阳性血清抑制。本试验通过RT-PCR扩增了5株NDV全长F基因并进行了序列测定。序列分析表明,5 株 NDV 的核苷酸序列分别为1662、1589、1676、1589和1662 bp,均含有1个开放阅读框,分別编码 553、528、553、520 和 553个氨基酸;根据基因裂解位点的氨基酸序列分析表明,鸡源XJ/10/08株属于强毒株, XJ/3/07株、XJ/7/07株、XJ/9/08株和XJ/11/08株属于弱毒株;同源性分析表明,5 个 NDV新疆分离株与 La Sota 疫苗株的核苷酸同源性在 83.7%～99.8% 之间,与 TaiWan95 株核苷酸同源性在 84.7%～93.3% 之间;遗传发育进化树分析表明, 分离到的4个弱毒株与LaSota、Clone30、B1、BEA-45在同一亚群,属基因Ⅱ型,强毒分离株与TaiWan95、广东株(GD-1-98)、江苏株(JS-5-1)在同一进化分支内,属基因Ⅶ型。 相似文献
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为研究近年国内的新城疫病毒（Newcastle disease virus,NDV）流行株与传统疫苗株（Mukteswar、La Sota及Clone 30）的亲缘关系和遗传演化情况,试验从广东、广西和海南地区患病的免疫鸡群病料中分离鉴定得到4株NDV流行株。通过RT-PCR技术对各毒株的F和HN基因进行扩增、克隆与序列分析,然后与GenBank上发表的NDV序列进行同源性比对、遗传进化树分析,并测定各毒株致病指数。结果显示,本试验中HN-08株的鸡胚半数感染量（EID50）、平均死亡时间（MDT）、脑内致病指数（ICPI）和静脉致病指数（IVPI）分别为10-8.37/0.2 mL、58.5 h、1.78和2.45,XX-08株分别为10-6.50/0.2 mL、75.0 h、1.61和2.41,YS-09株分别为10-7.75/0.2 mL、64.5 h、1.71和2.38,LF-09株分别为10-7.60/0.2 mL、63.8 h、1.84和2.38。4株鸡源NDV流行株彼此间F和HN基因推导的氨基酸序列同源性在95.8%以上,与鸡源流行株GX11/03、GM株的同源性为96.8%~98.6%,而疫苗株Mukteswar、La Sota及Clone 30株的同源性为86.7%~90.6%,与标准强毒株F48E9的同源性为88.4%~91.3%。表明4株NDV流行株均为强毒株,与近年来国内流行的GX11/03、GM株有较近的亲缘关系,而与传统疫苗株的关系相对较远。 相似文献
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LI Xiao-wen LIU You-ming LIANG Guo-zhi MEI Min-min HUANG Wen-jing LI Wen-feng HUANG Shu-jian LI Zhi-li 《中国畜牧兽医》2017,44(7):1925-1933
In order to study the relationship and evolution between the latest Newcastle disease virus (NDV) epidemic strain and the vaccine strain (Mukteswar,La Sota and Clone 30 strains),four NDV epidemic strains were initially isolated and identified from immunized chicken groups in Guangdong,Guangxi and Hainan provinces. Fusion (F) gene and hemaglutinin neuraminidase (HN) gene of four isolated stains were amplified,cloned and sequenced by RT-PCR method,and the homology analysis of amino acid sequences deduced by F and HN genes of NDV and other strains published in GenBank were studied, the phylogenetic tree were build,and the pathogenicity index of each strain (EID50,MDT, ICPI and IVPI) were determined. The results showed that the EID50,MDT,ICPI and IVPI of the HN-08 strain were 10-8.37/0.2 mL,58.5 h,1.78 and 2.45,that of the XX-08 strain were 10-6.50/0.2 mL,75.0 h,1.61 and 2.41,that of the YS-09 strain were 10-7.75/0.2 mL,64.5 h,1.71 and 2.38,and of the LF-09 strain were 10-7.60/0.2 mL,63.8 h,1.84 and 2.38.In this experiment,the similarity of amino acid sequences of F and HN genes was over 95.8% among four strains of NDV isolated from chicken,the similarity of four NDV strains with chicken epidemic strains (GX11/03 and GM strains),vaccine strains (Mukteswar,La Sota and Clone 30 strains) and standard virulent strain (F48E9 strain) were from 96.8% to 98.6%,86.7% to 90.6% and 88.4% to 91.3%,respectively. The results showed that four NDV epidemic strains were virulent strains,which were closely related to the GX11/03 and GM strains that were popular in China in recent years,but relatively far from the traditional vaccine strains. 相似文献
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13株新城疫病毒强毒株的分离鉴定及部分生物学特性 总被引：4，自引：0，他引：4
2004年-2005年从黑龙江省11个地区鸡场、鸽场、鹅场疑似新城疫(ND)病死鸡、鸽、鹅体内分离到24株具有血凝活性的病毒,经HA、HI试验和电镜观察证实了13株病毒为NDV。其鸡胚平均死亡时间(MDT)和1日龄雏鸡脑内接种致病指数(IC-PI)分别为36.8 h~73.6 h和1.51~2.00之间,属于NDV强毒株或中强毒株。除HLJ-12-04株外,强毒株的血凝素热稳定性较差,属快速或中速血凝解脱型;弱毒株的血凝素热稳定性好,属慢速血凝解脱型。所有毒株均可较好地凝集鸡和人(O型血)的红细胞,但对其他动物的红细胞凝集性差异较大,对马和兔的红细胞凝集无规律。 相似文献
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Characterization of a battery of monoclonal antibodies for differentiation of Newcastle disease virus and pigeon paramyxovirus-1 strains 总被引：6，自引：0，他引：6
Twenty monoclonal antibodies (MCAs) prepared against the velogenic GB-Texas strain of Newcastle disease virus (NDV) and the type 1 pigeon paramyxovirus (PPMV-1) were characterized and examined as potential immunodiagnostic reagents. All MCAs generated were found to bind specifically, but with varying reactivity, to various NDV strains in direct binding assays. In addition, MCA 15C4 neutralized and inhibited hemagglutination (HA) of all lentogenic, mesogenic, and velogenic NDV strains tested but not the PPMV-1 strain. Antibody 10D11 also inhibited HA activity, but inhibition was more selective and limited to the mesogenic and domestic or indigenous velogenic strains of NDV. MCA 79 reacted in all serologic assays with an antigenic site common to all serotype 1 avian paramyxoviruses. Passive immunization studies involving three different neutralizing MCAs (35, 79, and 15C4) showed that enhanced, but not complete, protection against virulent NDV challenge was provided when the three MCAs were administered in combination. 相似文献
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新城疫病毒F48E9株及东北地区流行株F基因遗传变异分析 总被引：16，自引：4，他引：12
采用异硫氰酸胍／酚／氯仿抽提一步法,提取新城疫病毒（ＮＤＶ）Ｆ４８Ｅ９株及２个东北地区分离株ＤＢ３、ＤＢ５基因组ＲＮＡ,并以其为模板经反转录合成Ｆ基因ｃＤＮＡ第１链,再利用ＰＣＲ技术分段增出Ｆ基因的ｃＤＮＡ。Ｆ４８Ｅ９、ＤＢ３和ＤＢ５株Ｆ基因的ｃＤＮＡ经酶切修饰后,插入ｐＵＣ１９的多克隆位点中,转化感受态Ｅ。ｃｄｉ ＤＨ５ａ,经相对分子质量比较、酶切分析及ＰＣＲ等鉴定方法筛选出Ｆ４８Ｅ９、ＤＢ３和 相似文献
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为确定西藏新城疫病毒(Newcastle disease virus,NDV)分离株HN基因结构特征及其与已知毒株的遗传相关性,本试验应用RT-PCR技术对西藏NDV分离株HN基因进行扩增,然后克隆至pMD18-T载体测序,并与国内外代表性毒株和当前疫苗株进行比对及系统发育分析。结果表明,NDV分离株HN基因片段长度为1734 bp, 编码577个氨基酸;推导的氨基酸序列均有5个糖基化位点, XZ10和XZ17有12个半胱氨酸残基,XZ20只有11个半胱氨酸残基。NDV西藏分离株HN基因与国内代表性毒株核苷酸同源性在81.1%~93.0%之间,氨基酸同源性在81.1%~93.6%之间;与疫苗株核苷酸同源性在87.8%~98.7%之间,氨基酸同源性在88.0%~98.9%之间。系统进化分析结果表明,NDV西藏分离株HN基因均属于Ⅱ型。 相似文献
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Twelve Newcastle disease virus (NDV) strains were isolated from chickens involved in outbreaks of Newcastle disease (ND) in western China (Shaanxi, Gansu, Xinjiang, Qinghai and Guangxi provinces) between 1979 and 1999. All strains were determined to be velogenic by plaque formation, the mean death time (MDT) of embryonated eggs, and the intracerebral pathogenicity index (ICPI). For preparation of virus RNA, the acid guanidinium-thiocyanate method was used. A 908bp fragment of nucleotide was amplified by RT-PCR starting from the N terminal of the F gene and the PCR segments were cloned into the PGEM-T vector and sequenced. The similarities of the nucleotide sequences (1-519bp) and predicted amino acid sequences of the F gene (1-125) were analyzed by comparing the 12 NDV isolates with the NDV vaccine strains Lasota, B1, H1 and V4, with classical NDV strains and recent epizootic strains. Phylogenetic analysis demonstrated that all strains were of two novel genotypes; the NDV strains that caused the outbreak of ND in western China during 1998-1999 was of the genotype VIIa, whereas the strains from the Qinghai province (1979-1985) were of genotype VIII, which has been found predominately in southern Africa. 相似文献