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1.
饲料蛋白水平对史氏鲟幼体消化率的影响   总被引:4,自引:0,他引:4  
本实验研究了饲料蛋白水平对史氏鲟 (Acipenserschrenki)幼体消化率的影响。试验设计了四个饲料蛋白水平 ,分别为 :2 8%、 32 %、 36 %、 41 %。结果表明饲料的蛋白质含量对史氏鲟幼体总消化率 (干物质消化率 )影响不显著 ,但对饲料中各主要营养成分的消化率有明显作用。饲料蛋白质含量对鲟鱼幼体的蛋白消化率影响极显著 ,二者呈三次曲线关系 :y=0 92 +0 0 0 0 5 (x - 36) 2 +4 44(x - 36) 3,饲料蛋白含量 36 %的试验组幼鲟对蛋白质的消化率最高 ,达到 93 % ;鲟鱼对饲料粗脂肪的消化率也受到饲料蛋白水平的显著影响 ,蛋白含量为 32 %和 36 %的两个试验组对脂肪的消化率显著高于其他两组 ;幼鲟对糖类的消化率同饲料蛋白水平间呈显著的对数曲线关系 :y=1 90 3 - 84 2lgx。综合分析认为 ,当饲料中的蛋白含量在 36 %时 ,幼鲟对饲料各营养物质的消化率最高 ,所以幼鲟饲料的蛋白含量控制在 36 %左右比较适宜  相似文献   

2.
饲料蛋白质水平对俄罗斯鲟幼鲟生长的影响   总被引:2,自引:0,他引:2  
邢西谋 《淡水渔业》2003,33(4):14-16
设计 5种不同粗蛋白质水平的饲料 ,分别投喂平均体重约为 4 2 9g的俄罗斯鲟幼鲟 ,试验时间 4 0天。结果表明 ,幼鲟对饲料中粗蛋白质的最适需要量为 4 2 %。当饲料粗蛋白质水平低于 4 2 %时 ,随着饲料中粗蛋白质水平的增加 ,幼鲟的生长速度和饲料转换率都显著增加。当饲料中粗蛋白质水平达到和超过 4 2 %时 ,随着饲料粗蛋白质水平的增加 ,幼鲟的生长速度和饲料转换率没有明显变化。  相似文献   

3.
中华鲟幼鲟的适宜投喂率研究   总被引:15,自引:0,他引:15  
通过4周的生长试验来确定中华鲟幼鲟的适宜投喂率。试验饲料是一种半湿性的配合饲料,日投喂率分别为鱼体重的2%,4%,6%,8%,根据最大增重率和最小饲料系数这一标准,在水温14℃的条件下,中华鲟幼鲟的适宜投率应是体重的2%。  相似文献   

4.
中华鲟幼鱼饲料营养素适宜含量的研究   总被引:9,自引:1,他引:8  
用蛋白质梯度饲养法测定蛋白质适宜含量范围,以增重率为指标,采用直线和抛物线回归,计算得出幼鲟饲料蛋白质适宜需要量为35 .41 % ~49 .09 % ;用正交试验法,以生长比速、蛋白质效率和饲料系数为评价指标,筛选幼鲟最适生长的饲料营养素含量为:蛋白质40 .41 % ,脂肪9 .06 % ,糖25 .56 % ,纤维素4 .08 % 。各营养素对幼鲟的生长及蛋白质效率的影响大小为:蛋白质> 脂肪> 糖> 纤维素,其中蛋白质为影响生长和鱼体成份变化的主要因素( P< 0 .05) ;用脂肪梯度饲养法对正交试验结果进一步重复和验证,结果吻合。  相似文献   

5.
暂养2006年5月渔民在崇明东滩误捕受伤的中华鲟野生幼鲟,分析研究了其6—8月的生长特点。结果显示,抢救存活的中华鲟幼鲟在人工环境下,体长和体重分别从(15.40±0.64)cm和(26.00±1.34)g增加到(29.20±0.75)cm和(108.00±3.87)g,日均增长1.54 mm,日平均增重0.90 g。拟合中华鲟幼鲟体长与体重的关系为:W=0.121 5 L2.031 3(R2=0.945 7),其中b小于3,为异速生长,说明其体长增长快于体重增长。与同期野生环境下的中华鲟幼鲟比较,幂指数系数b人工=2.0313相似文献   

6.
周贵谭 《水利渔业》2004,24(1):54-55
用蛋白质梯度法,以白鱼粉为蛋白源,对稚鳖蛋白质需求进行测定。试验期间水温28 3℃,420只稚鳖平均体重(11 23±0 93)g,分成7个组,每组3个重复,饲养60d。结果表明,蛋白质质量分数50 91%、54 81%生长较好,稚鳖饲料粗蛋白适宜质量分数应为49 52%。  相似文献   

7.
史氏鲟幼鲟对饲料中磷的需要量   总被引:1,自引:1,他引:0  
文华  严安生  高强 《水产学报》2008,32(2):242-248
通过生长饲养试验研究史氏鲟幼鲟对饲料中磷的需要量.以Ca(H2PO4)2·H2O为磷源,配制含磷水平分别为0.18%(对照组),0.40%,0.71%,0.98%,1.38%和1.66%的6组等能等氮纯化饲料,每组饲料饲喂3个重复,每个重复饲养20尾初体质量约为4.7 g的幼鲟.经过8周的饲养后,对生长、饲料效率、成活率、幼鲟肌肉常规组成及脊椎骨灰分和钙磷含量进行分析.结果表明,随饲料中磷含量的升高,鱼体增重率、饲料效率和特定生长率显著增加(P<0.05),直至0.98%组达最大,然后随着饲料磷含量的增加,这些指标显著降低(P<0.05);试验各添加组的成活率显著高于对照组(P<0.05),但各添加组间没有显著差异(P>0.05);饲料添加磷对鱼体肌肉水分、粗蛋白质和粗脂肪含量无显著影响(P>0.05);当饲料磷含量在0.71%~1.66%之间时,脊椎骨中灰分、钙和磷含量差异不显著(P>0.05),但显著高于对照组和0.40%试验组(P<0.05).经过折线回归分析得出,为维持史氏鲟幼鲟最佳生长和骨骼磷水平,饲料中磷的需要量为0.88%~1.00%(占干饲料).  相似文献   

8.
在葛洲坝下中华鲟(Acipenser sinensis)产卵场连续两年未监测到自然繁殖的情况下,2015年6月在江苏溆浦段发现15尾疑似中华鲟幼鱼。本研究通过形态学和分子生物学方法对该15尾幼鲟进行鉴定。形态学度量结果显示,除1尾个体外,新发现的14尾幼鲟与历史记载的中华鲟野生幼鱼生物学性状完全一致,但其体长和体重低于历史同期出现在该区域的中华鲟。同时采用11个多态微卫星位点,对15尾幼鲟样本和分布于我国的3种鲟鱼,包括30尾中华鲟(A.sinensis)、39尾达氏鲟(A.dabryanus)和33尾施氏鲟(A.dabryanus)进行遗传分析,发现这15尾幼鲟与中华鲟的遗传距离最近(F_(st)=0.039,P0.05),其次为达氏鲟(F_(st)=0.108,P0.05),最远为施氏鲟(F_(st)=0.209,P0.05),从遗传和进化的角度判定该批幼鱼为中华鲟,该结果暗示中华鲟在葛洲坝下游极有可能找到了新的产卵场,为中华鲟的繁殖群体和幼鱼的种群监测提供了依据。遗传多样性分析显示,该批鱼的遗传多样性(SW=1.998)略低于野生中华鲟(SW=1.886),暗示中华鲟幼鱼遗传多样性水平降低,野生亲本的数量仍不容乐观。  相似文献   

9.
为探索饲料蛋白质水平对克氏原螯虾生长性能的影响,选用体质健壮、规格一致的克氏原螯虾[初始体质量为(9.45±1.33) g]225尾随机分成5组,每组设3个重复,每个重复15尾虾,分别饲喂5种不同蛋白质水平(质量分数为25%、30%、35%、40%、45%)的饲料,试验期为60 d。试验结果显示:当饲料中蛋白质质量分数由25%提高至45%时,试验虾的体质量增长率(WGR)先升高后降低,而饲料系数(FCR)呈现先降低后升高的变化趋势;当饲料蛋白质质量分数为30%时,克氏原螯虾的WGR和特定生长率(SGR)均达到最大值,显著高于除饲料蛋白质质量分数为35%外的其他各组(P0.05); FCR在饲料蛋白质质量分数为30%时最低,显著低于除35%和40%组外的其他各组(P0.05)。通过二次曲线方程回归分析,当饲料蛋白质质量分数分别为31.66%、31.37%和32.20%时克氏原螯虾的WGR、SGR和FCR达到最优。结果表明,在该试验条件下,克氏原螯虾饲料中适宜的蛋白质质量分数为31.37%~32.20%。  相似文献   

10.
<正>三、蛋白质(一)最佳饲料蛋白质关于最佳饲料蛋白含量已有的报道如下:高首鲟(初重145g)为40.5%(Mooreet al.,1988),西伯利亚鲟(初重20g~40g)40%(Medale etal.,1995),中华鲟(初重8g~34g)40%~45%(Xiao et  相似文献   

11.
The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.  相似文献   

12.
13.
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.  相似文献   

14.
In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.  相似文献   

15.
16.
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus.  相似文献   

17.
Growth hormone (GH) and reproduction: a review   总被引:5,自引:0,他引:5  
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations.  相似文献   

18.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.  相似文献   

19.
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality).  相似文献   

20.
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