共查询到19条相似文献，搜索用时 218 毫秒
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REV感染肉种鸡后动态病理学观察与抗原分布及检测 总被引：1，自引：0，他引：1
用网状内皮组织增生病病毒(REV)人工接种1日龄肉种鸡,定期剖杀,通过病理组织学观察病变特征及肿瘤的发生发展;用免疫组化、PCR检测抗原与病毒在体内各器官的分布规律;超薄切片观察肿瘸细胞、病毒的形态.结果显示,肝脏、脾脏、胸腺、法氏囊较早出现明显的肿瘤增生灶,在这些器官内免疫组化阳性信号出现最早,且信号最强,在5周龄时,心肌、腺胃、肺、肾、骨髓等器官才开始出现阳性信号,但呈中度的阳性反应,9周龄后阳性信号开始减弱.肝脏、脾脏、胸腺、法氏囊、骨髓扩增出REV的LTR基因片段,超薄切片显示病毒多分布在胞浆内和细胞间隙内.研究发现病变明显的组织中,阳性反应较强,肿瘤细胞增生明显,即强阳性反应的组织器官易形成肿瘤转移灶及肿瘤结节,同时,抗原最早出现在这些器官说明其中可能存在REV的靶细胞.虽然阳性信号在腺胃出现较晚,但90%以上的腺胃肿大说明腺胃也是REV的主要侵害器官. 相似文献
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商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病理学初报 总被引：21，自引：10，他引：11
2007年7月,山东某鸡场8 000余只150日龄海兰褐鸡发病,病鸡特征性表现衰竭、消瘦和血管瘤。大体病变：内脏器官肿大,表面及切面可见大小不等的灰白色结节;血管瘤大小不等、富有弹性,内含大量血液,血液内富含成髓细胞;组织学病变：肝脏、脾脏、骨髓、肠管和胰腺浆膜面有大量的胞质含嗜碱性颗粒的成髓细胞增生,同时在肠管固有层出现明显的肠腺上皮细胞和网状细胞增生,腺胃固有层出现明显的网状细胞增生,血管瘤为典型的海绵状血管瘤,可见其发展的各个阶段;免疫组织化学检测：经REV与ALV-J单克隆抗体检测发现,REV抗原阴性,而ALV-J抗原强阳性。在骨髓、肝脏及其它组织内的成髓细胞内、血管瘤海绵状壁层内皮细胞、增生的肠腺上皮细胞、腺胃腺小管上皮细胞、网状细胞内及部分间质内可见强阳性信号。因此,此病为成髓细胞瘤、血管瘤型并伴发多种肿瘤的J亚群白血病,这在国内外尚未见报道。 相似文献
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清远麻鸡禽白血病病理组织学观察和PCR诊断 总被引：2，自引：1，他引：1
本试验选择临床症状明显、经禽白血病抗原检测为阳性的15只祖代清远麻鸡,采集肝脏、脾脏、肾脏等器官进行病理组织学观察和PCR诊断。结果显示,从病理组织切片中观察到肝脏、肾脏、脾脏、骨髓、腺胃、心脏、肺脏、胰腺和卵巢等组织中有大量的淋巴母细胞呈灶状增生或散在分布,以及少量的成髓细胞增生。禽白血病病毒主要侵害祖代清远麻鸡机体的内脏器官和骨髓,对大脑等神经组织损害较轻;PCR扩增结果表明,所采集的15份样品均能扩增出545 bp左右的特异性片段,证明15份组织样品中均存在J亚型禽白血病病毒。 相似文献
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鸭源副黏病毒的致病性试验 总被引：1，自引：0，他引：1
利用鸭源副黏病毒(DPMV)SDFC株分别人工感染21日龄健康非免疫樱桃谷肉鸭和SPF雏鸡,观察试验鸡、鸭的发病情况及临床症状,于感染后不同时间剖杀,观察各组织器官的病理变化,并进行病理组织学研究。结果显示,鸭感染DPMV后发病率为100%,死亡率为43.3%;而鸡感染后的发病率、死亡率均为100%。病理组织学研究结果显示,鸭感染DPMV后,以心脏、肺脏、肝脏、肾脏、脾脏、法氏囊广泛性出血、变性为特征,消化道病变较轻微;而鸡感染DPMV后,各组织器官广泛性出血,脾脏、胸腺、法氏囊、肝脏、胰腺、肾脏、心肌组织细胞出现不同程度的变性、坏死;食管、腺胃和各段肠管广泛性出血,并伴有黏膜脱落。结果表明,DPMV对鸡、鸭均有较强的致病性,而与鸡相比,对鸭的致病性较弱。 相似文献
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禽白血病病毒J亚群(ALV-J)的攻毒试验 总被引：2，自引：1，他引：1
将禽白血病病毒 J亚群 (AL V- J)内蒙株 NM876 1和 NM9996人工接种于 1日龄爱维茵肉种鸡 ,通过眼观、病理组织学检查观察了攻毒鸡的病理学特征 ;通过 PCR和 EL ISA技术检测了病毒感染率、抗体变化规律以及病毒和抗体的相关性。结果显示 ,攻毒鸡从第 3周开始即可检出病毒 ,NM876 1株和 NM9996株病毒感染率分别为 71.4 %和6 4 .3% ;从第 5周开始 ,出现较明显的病理学变化 ,病变特征以骨髓、肝脏、心脏、脾、卵巢等组织内髓细胞增生为主 ,而法氏囊、脑、坐骨神经无变化 ;攻毒鸡抗体消长变化有一定的规律 ,一般在 7～ 8周龄和 18～ 2 0周龄时分别出现 1次高峰 ,而在 4～ 6周龄、10周龄和 2 3周龄分别出现 1次低谷 ,提示鸡场进行 EL ISA检测时要避开这一时期 ;攻毒鸡产生耐受性病毒血症 ,即病毒阳性而抗体阴性 (V A- )的比例较高 (7/14 ,9/14 )。通过以上的研究证明 ,AL V- J内蒙株疾病模型复制成功 ,NM876 1、NM9996可作为原型株进行相关研究 相似文献
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本试验旨在检查中药颗粒剂对传染性法氏囊病(IBD)鸡免疫器官损伤的修复作用。采用病理组织学方法检查传染性法氏囊病自然耐过鸡和用中药颗粒剂治疗而康复鸡的免疫器官,采用光学显微镜对其主要免疫器官进行病理组织学观察。即随机抽取中药颗粒剂治疗组和自然耐过对照组25日龄鸡各10只,其中中药治疗康复后35日龄鸡10只,自然耐过35日龄鸡7只,死亡3只。扑杀被检鸡,采集各鸡法氏囊、脾脏和肝脏病料,眼观病变以后,用10%中性福尔马林固定,石蜡包埋,切片,苏木素-伊红染色,组织切片,显微镜检查病理组织学变化,并进行显微组织拍照直观比较自然耐过鸡和自制中药颗粒治疗康复鸡的免疫器官病理组织学变化。被检的IBD自然耐过鸡的免疫器官仍呈轻度病理性变化,而采用自制中药颗粒治疗痊愈鸡的病理变化基本一致,表现为免疫增强。中药颗粒剂能够显著修复IBD鸡免疫器官的损伤。 相似文献
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本研究分别用免疫组织化学(IHC)法、间接免疫荧光(IFA)法对肿瘤病鸡不同脏器中的禽网状内皮增生病病毒(REV)进行了检测,旨在为两种REV抗原定位方法的应用提供实验依据.采用REV SD1005分离株人工接种1日龄海兰褐鸡,分别采取28日龄肿瘤病鸡的肿瘤、法氏囊、骨髓、肝脏、心脏、脾脏、腺胃的新鲜切面在洁净盖玻片上触片,同时制备其病理组织连续切片,分别用IHC法、IFA法对触片和切片进行REV检测.结果显示,两种用于抗原定位的染色方法检测结果完全一致,均可对病鸡的肿瘤、法氏囊、骨髓、肝脏、心脏、脾脏、腺胃等组织中存在的REV进行定位,依据染色后的阳性细胞感染率可判断REV的组织嗜性.本研究表明,IHC法与IFA法均可用于检测不同脏器中的REV,并具有良好的特异性、低背景和简便快速的特点.但相对于IFA法,IHC法更经济实用并且实验材料易于保存,因此可以在REV抗原定位研究中推广应用. 相似文献
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《中国兽医学报》2017,(9):1753-1755
将30只1日龄SPF鸡随机分为2组,其中攻毒组20只,对照组10只。攻毒组按每只0.2mL腹腔注射禽腺病毒4型(FAV-4)病毒液,对照组不做处理,隔离饲养,临床观察2周。攻毒后第3天攻毒组开始出现死亡,将死亡鸡解剖观察各器官大体病变,经聚合酶链式反应(PCR)检测为FAV-4后,采集心脏、肝脏、脾脏、肾脏、肺脏制作石蜡切片,观察病理组织学变化。结果显示,攻毒组死亡鸡只无明显临床症状而突然倒地死亡;主要剖检病变为心包积液、肝脏肿大,个别可出现脾脏肿大、肺脏淤血、肾脏肿大;病理组织学变化为肝脏、肺脏、肾脏淤血,细胞变性坏死;而对照组无任何异常症状和病理组织学变化。 相似文献
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J亚群白血病的病理学观察及PCR诊断 总被引：4，自引：1，他引：3
从某肉种鸡场取疑似J亚群白血病的自然发病鸡，剖检，观察病理学特征(光镜、电镜)。随后对该场进行ALV-J抗体ELISA检测，发现感染率达28％，从中选取部分抗体阳性鸡及阴性鸡剖检，取肝进行ALV-J特异性PCR检测。结果：自然发病鸡病变明显，在肝、脾、肾、睾丸(卵巢)肺等多种组织肿大，并有大小不等的灰白色结节。镜检：病灶内及肿瘤主要由密集的髓细胞组成，在大脑、小脑坐骨神经中未见。电镜，肿块中的髓细胞样瘤细胞呈圆形、核圆形或椭圆形，体积大小不一、染色质边集，胞浆中溶酶体增多，有些电子密度较高，有些趋于溶解，肝细胞体积增大，核浓缩或淡染，胞浆中线粒体增多，肿胀，嵴减少或消失，在胞浆膜下有病毒粒子存在。PCR结果：6例抗体阳性鸡和部分自然病例PCR阳性而2只对照鸡PCR阴性。通过以上证据可知，病变明显的和抗体阳性的鸡PCR结果全部为阳性，证明鸡体内病毒与抗体共存，而抗体阴性，病毒阳性的结果未出现，说明此肉种鸡场感染的ALV-J大部分是由于水平传播造成的。 相似文献
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蛋鸡中发现J亚群白血病与网状内皮增生症自然混合感染 总被引：12，自引：3，他引：12
发病蛋鸡经组织学、免疫组化检测确诊为J亚群白血病与网状内皮增生症混合感染。与人工接种病例不同的是，在肿瘤组织内还发现一种特殊的细胞——淋巴-巨噬细胞；在骨髓和肿瘤组织中检测到部分髓细胞胞浆内有ALV—J抗原表达。从发病情况、各器官病变程度及免疫组化结果来看，2种病原存在明显的相互协同作用，脾可能是网状内皮增生症的原发器官。但其发病的时间可能不如J亚群白血病早。此次在蛋种鸡发现此混合感染提示，病毒在环境选择压及免疫选择压的作用下，其生物特性、致病作用以及宿主范围均可发生改变。应警惕J亚群白血病和网状内皮增生症混合感染在蛋鸡中的大面积暴发。 相似文献
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The amino acid content of the liver, brain, serum and leg muscle of broiler breeder female chicks was not influenced appreciably at 4 and 8 weeks of age by feeding a diet containing sub‐optimum levels of lysine. The feeding of the low‐lysine diet produced a slight effect on the amino acid content of the breast muscle and a decided effect on the amino acid content of the skin and bone marrow at 4 and 8 weeks of age. The amino acid content of the heart tissue from chicks which received the 0.53% lysine diet was higher at 4 weeks of age. No explanation for this observation can be offered. The more decided changes were produced in the bone marrow of the chicks fed the low‐lysine diet at 4 and 8 weeks of age.
There is a possibility that feeding the low‐lysine diet had an effect on protein synthesis in the bone marrow. Chicks fed the low‐lysine diet had apparently overcome the lysine deficiency in the synthesis of protein of the bone marrow at the end of the 12th week. 相似文献
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生物素—亲和素法检测鸡实验性成髓细胞性白血病的研究 总被引：2，自引：2，他引：0
用生物素-亲和素法检测实验鸡雏47只。结果,感染后鸡雏骨髓的成髓细胞是鸡成髓细胞性白血病病毒最先攻击的靶细胞。死亡病例,阳性成髓细胞分布于骨髓、肝、脾、肾、心、肺、胸腺、法氏囊、盲肠扁桃体、翅羽髓等器官。60天病例,骨髓、肝、心壁内形成阳性成髓细胞结节。尸体的骨髓、肝脏和翅羽髓任一器官,在其血管内外检出阳性成髓细胞聚集和增生,可诊断为鸡成髓细胞性白血病。 相似文献
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Takami S Goryo M Masegi T Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(1):13-18
The major organs and tissues of 24 broiler chickens (70 or 71 days old) suspected of spindle-cell proliferative disease (SPD) because of showing the tumorous lesions distributed throughout the body at meat inspection were collected for histopathological and immunohistochemical examination. Macroscopically, liver, spleen and cecal tonsil showed severe enlargement and white nodules or plaques were observed in the liver, spleen, kidney, intestine and bone marrow of the femur. All chickens were diagnosed with SPD based on the histopathological examination. The lesions of SPD were observed in the liver, spleen, kidney, heart, lung, pancreas, proventriculus, gizzard, duodenum, jejunum, ileum, rectum, cecal tonsil, bursa of Fabricius, bone marrow of the femur and skin. Hemangioma was observed in the lung of 1 bird. Eight 1-day-old specific pathogen-free chicks were inoculated intraperitoneally with 0.25 ml of a 20% homogenate of the affected spleens of three naturally occurring cases. One inoculated bird, necropsied at 10 weeks of age, macroscopically had a white nodule in the kidney and histopathologically had spindle-cell proliferative lesions, a pattern similar to that seen in the naturally occurring cases, in the liver, spleen, kidney, heart, lung, pancreas, proventriculus, duodenum, cecal tonsil and bone marrow of the femur, and was diagnosed with SPD. Immunohistochemically, significant positive reactions with a rabbit antiserum against avian leukosis virus antigens were detected in all spindle cells in the proliferative lesions of all examined SPD cases and in tumor cells of the hemangioma of a field case. 相似文献
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I Vlemmas P Wohlsein G Trautwein G Kanakudis T Tsangaris 《Berliner und Münchener tier?rztliche Wochenschrift》1990,103(12):422-425
Formalin-fixed tissue samples obtained from puppies experimentally infected with canine parvovirus type 2 (CPV-2) were investigated immunohistologically with the direct immunoperoxidase method. Besides cardiac muscle and lymphatic tissues, bone marrow, intestine, liver, kidney, pancreas and to a lesser degree lung stained positive for viral antigen and are considered as sites of viral replication. The distribution of viral antigen reveals a tropism of CPV-2 to numerous organs in puppies infected during the first week of life. 相似文献
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Pork products are a possible source of introduction of PCV2 isolates into a pig population. However, limited work has been done on the transmission through meat of porcine circovirus type 2 (PCV2), a virus associated with several disease syndromes in pigs. The objectives of this study were to determine if pork products from PCV2-infected pigs contain PCV2 DNA/antigen and to determine if the PCV2 present in the tissues is infectious by performing in vitro and in vivo studies. Skeletal muscle, bone marrow, and lymphoid tissues from pigs experimentally inoculated with PCV2 were collected 14 days post-inoculation (DPI). The tissues were tested for presence of PCV2 DNA by quantitative real-time PCR, for PCV2 antigen by immunohistochemistry (IHC), and for presence of infectious PCV2 by virus isolation and inoculation of PCV2 naïve pigs. Lymphoid tissues contained the highest amount of PCV2 (positive by PCR, IHC, and virus isolation), bone marrow contained a lower amount of PCV2 (positive by PCR and IHC but negative by virus isolation), and skeletal muscle contained the lowest amount of PCV2 (positive by PCR but negative by IHC and virus isolation). Naïve pigs fed for three consecutive days with either skeletal muscle, bone marrow, or lymphoid tissues all became PCV2 viremic as determined by quantitative real-time PCR on serum starting at 7 DPI. The pigs also seroconverted to PCV2 as determined by PCV2 IgM and IgG ELISA. In addition, PCV2 antigen was detected by IHC stains in lymphoid tissues and intestines collected from the majority of these pigs. Results from this study indicate that uncooked PCV2 DNA positive lymphoid tissues, bone marrow, and skeletal muscle from PCV2 viremic pigs contain sufficient amount of infectious PCV2 to infect naïve pigs by the oral route. 相似文献