首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 62 毫秒
1.
将360只1日龄天府肉鸭随机分为6组，分别喂以对照日粮（Cu8mg／kg）和高铜日粮（I组：Cu100mg／kg；Ⅱ组：Cu200mg／kg；Ⅲ组：Cu400mg／kg；IV组：Cu600mg／kg；V组：Cu800mg／kg）6周，研究日粮铜添加水平对雏鸭肝及其相关生化指标的影响。结果显示，Ⅱ组、Ⅲ组、Ⅳ组、V组雏鸭肝出现不同程度的病理损伤，肝细胞颗粒变性、空泡变性。Ⅳ组、V组肝铜含量和血清铜含量均显著高于对照组（P〈0．01）。Ⅲ组、Ⅳ组和V组血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高（P〈0．01）。结果表明，日粮铜含量达400～800mg／kg即可引起肝的病理损伤。  相似文献   

2.
高铜对雏鸡肝细胞周期和凋亡影响的研究   总被引：5，自引：2，他引：5  
420只1日龄艾维菌肉鸡随机分为7组，分别喂以对照日粮（Cu 11mg／kg）和高铜日粮（Cu 100mg／kg，高铜Ⅰ组；Cu 200mg／kg，高铜Ⅱ组；Cu 300mg／kg，高铜Ⅲ组；Cu 400mg／kg，高铜Ⅳ组；Cu 500mg／kg，高铜Ⅴ组；Cu 600mg／kg，高铜Ⅵ组）6周，以流式细胞术和原位末端标记法（TUNEL）研究了高铜对雏鸡肝细胞周期和凋亡的影响。结果表明，日粮铜水平达到300mg／kg以上时，肝细胞DNA直方图出现位于G1峰之前的亚G1峰（又叫AP峰），凋亡细胞比例与对照组比较差异显著（P〈0．05）。TUNEL法检测，肝脏组织切片中出现被染成黄褐色的凋亡细胞，且检出率随日粮铜水平的升高而升高。日粮铜水平达到400mg／kg以上时，肝细胞增殖分化受阻。  相似文献   

3.
高铜对雏鸭抗氧化酶活性的影响   总被引：1，自引：1，他引：1  
360只1日龄天府肉鸭随机分为6组,分别喂以对照日粮(Cu 8 mg/kg)和高铜日粮(Ⅰ组Cu 100 mg/kg;Ⅱ组Cu 200 mg/kg;Ⅲ组Cu 400 mg/kg;Ⅳ组Cu 600 mg/kg;Ⅴ组Cu 800 mg/kg),试验期6周.与对照组比较,高铜Ⅳ组和Ⅴ组血清铜锌SOD、CP活性、肝脏铜锌SOD、GSH-Px活性降低(P<0.01);高铜Ⅲ组、Ⅳ、Ⅴ组肝脏黄嘌呤氧化酶、单胺氧化酶含量显著升高(P<0.01);高铜Ⅰ、Ⅱ组血清铜锌SOD、CP活性、肝脏铜锌SOD、GSH-Px活性升高(P<0.01).结果表明,日粮铜水平达400 mg/kg及以上时可引起血清和肝脏抗氧化酶活性降低,使机体抗氧化功能受损.  相似文献   

4.
日粮中添加高水平铜对雏鸭血液指标的影响   总被引：1，自引：0，他引：1  
试验对日粮中添加高水平铜对雏鸭血液指标的影响进行了研究。360只1日龄天府肉鸭随机分为6组，分别喂以对照日粮（Cu 8mg／kg）和高铜日粮（Cu100mg／kg，高铜Ⅰ组；Cu200mg／kg，高铜Ⅱ组；Cu400mg／kg，高铜Ⅲ组，Cu600 mg／kg，高铜Ⅳ组；Cu800mg／kg，高铜V组）6周。与对照组比较，高铜Ⅳ组和V组的红细胞数量、血红蛋白含量、血清铜蓝蛋白活性和血清铜锌SOD活性降低（P〈0．0）1），高铜Ⅲ组、Ⅳ组和V组血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高（P〈0．01），胆固醇、甘油三酯和总胆红素含量显著增加（P〈0．01）。结果表明．日粮铜水平为400mg／kg及以上时可引起上述血液指标的改变。  相似文献   

5.
为探讨日粮铜添加水平对雏鸡肝脏及相关血液生化指标的影响,将420只1日龄艾维因肉鸡随机分为7组,分别喂以对照日粮（Cu 11mg／kg）和高铜日粮（Cu 100mg／kg,高铜Ⅰ组;Cu 200mg／kg,高铜Ⅱ组;Cu 300mg／kg,高铜Ⅲ组,Cu 400mg／kg,高铜Ⅳ组;Cu 500mg／kg,高铜V组;Cu 600mg／kg,高铜Ⅵ组）6N。高铜Ⅱ组、Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组雏鸡肝脏出现不同程度的病理损害,肝细胞颗粒变性和脂肪变性;透射电镜观察,肝细胞线粒体肿胀,嵴断裂甚至溶解消失。除高铜Ⅰ组和Ⅱ组外,其余高铜组肝脏铜含量均显著高于对照组（P〈0．01）。高铜Ⅴ组和Ⅵ组丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高（P〈0．05）。结果表明,日粮铜含量200～600mg／kg可引起肝脏病理损伤。  相似文献   

6.
选用1日龄艾维茵肉鸡健雏420只,随机分为7组,分别饲以含铜11 mg/kg的基础日粮和含铜量分别为100（Ⅰ）、200（Ⅱ）、300（Ⅲ）、400（Ⅳ）、500（Ⅴ）、600 mg/kg（Ⅵ）的6种高铜日粮6周,探讨高铜对雏鸡某些血液生化指标的影响。结果显示：高铜Ⅰ组的血清铜蓝蛋白与铜锌SOD酶活性显著升高（P〈0.05或P〈0.01）;高铜Ⅵ组的红细胞数量、血红蛋白含量和血清铜蓝蛋白活性降低,与对照组比较差异显著（P〈0.05）;高铜Ⅳ、Ⅴ和Ⅵ组的血清天门冬氨酸氨基转移酶、丙氨酸氨基转移酶活性、血清尿素氮和肌酐含量,血清铜和肝铜含量显著升高（P〈0.05或P〈0.01）。  相似文献   

7.
高铜对雏鸭肾脏细胞周期和凋亡影响的研究   总被引：2，自引：1，他引：2  
360只1日龄天府肉鸭随机分为6组,分别喂以对照日粮（Cu 8 mg/kg）和高铜日粮（Cu 100 mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 400 mg/kg,高铜Ⅲ组;Cu 600 mg/kg,高铜Ⅳ组;Cu 800 mg/kg,高铜Ⅴ组）6周,以流式细胞术研究高铜对雏鸭肾脏细胞周期和凋亡的影响。结果显示,肾脏细胞的调亡比例随日粮铜水平的升高而升高,当日粮铜水平达到400 mg/kg以上时,肾脏细胞凋亡比例与对照组比较差异极显著（P〈0.01）。日粮铜水平达到400 mg/kg以上时,肾脏细胞的增殖指数（PI）极显著低于对照组（P〈0.01）。结果表明日粮铜水平达到400 mg/kg以上时会引起肾脏受损,肾脏细胞的增殖分化严重受阻,肾脏细胞凋亡大量增加。  相似文献   

8.
在对照组（Cu 11mg／kg，A组）基础上设高铜组3个（110mg／kg、220mg／kg和330mg／kg，分别对应为试验组B、C、D），试验期为60d，在12、24、36、48、60日龄采集肝组织，提取线粒体，测定线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ的活性变化。结果表明，11mg／kg和110mg／kg铜日粮添加组线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ的活性呈增加趋势。220mg／kg和330mg／kg铜日粮添加组肉鸡肝细胞线粒体复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ活性减弱，但主要在试验后期，尤其是48日龄之后，比对照组和各组内的试验前期下降明显（P〈0．05）。说明高铜日粮在一定程度上对线粒体复合物活性是有利的，但过高的铜添加到日粮中（≥220mg／kg）可降低肉鸡肝细胞线粒体呼吸链复合物的功能活性。据此可以推测，线粒体是机体铜过量状态下侵害的靶部位之一，而且能作用于线粒体各个复合物，造成线粒体呼吸功能减弱，呼吸链中电子传递发生故障，从而影响线粒体功能的正常发挥。  相似文献   

9.
高铜对雏鸡脑组织氧化状态的影响   总被引：1，自引：1，他引：0  
1日龄艾维茵肉鸡健雏360羽,随机均分为6组,分别喂以对照日粮(10.89 mg/kg)和高铜日粮(Cu 100 mg/kg,高铜Ⅰ组;Cu 200 mg/kg,高铜Ⅱ组;Cu 400 mg/kg,高铜Ⅲ组;Cu 600 mg/kg,高铜Ⅳ组;Cu 800 mg/kg,高铜Ⅴ组)6周,观察高铜对脑组织氧化状态的影响.高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组脑组织MDA含量升高,与对照组比较差异显著或极显著(P<0.05或P<0.01);脑组织铜-锌-超氧化物歧化酶(Cu-Zn-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,高铜Ⅰ、Ⅱ组显著或极显著高于对照组(P<0.05或P<0.01),高铜Ⅲ、Ⅳ和Ⅴ组较对照组极显著降低(P<0.01);高铜Ⅱ、Ⅲ、Ⅳ和Ⅴ组脑组织羟自由基活性升高,与对照组比较差异显著或极显著(P<0.05或P<0.01).同时,血清的MDA含量及Cu-Zn-SOD和GSH-Px的活性变化与脑组织一致.结果表明,日粮铜水平在400 mg/kg及以上时,脑组织的抗氧化功能受损.  相似文献   

10.
本研究旨在探讨日粮铜水平对雏鸭肾脏抗氧化功能的影响。360只1日龄天府肉鸭随机分为6组,分别喂以对照日粮（Cu 8 mg.kg^-1）和高铜日粮（Cu 100 mg.kg^-1,高铜Ⅰ组;Cu 200 mg.kg^-1,高铜Ⅱ组;Cu 400mg.kg^-1,高铜Ⅲ组;Cu 600 mg.kg^-1,高铜Ⅳ组;Cu 800 mg.kg^-1,高铜Ⅴ组）6周。高铜Ⅱ组、Ⅲ组、Ⅳ组和Ⅴ组肾脏组织丙二醛含量、游离脂肪酸含量及羟自由基含量显著升高（P〈0.01）,高铜Ⅴ组肾脏CuZn-SOD活性显著降低（P〈0.01或P〈0.05）。同时,高铜Ⅲ、Ⅳ和Ⅴ组血清铜蓝蛋白（CP）活性和高铜Ⅴ组血清CuZn-SOD活性显著降低（P〈0.01或P〈0.05）,高铜Ⅲ、Ⅳ组血清CuZn-SOD活性降低,但不显著（P〉0.05）。结果表明,日粮铜含量达到或超过400 mg.kg^-1可引起肾脏和血清抗氧化酶活性降低,抗氧化功能受损,肾脏组织自由基和过氧化产物产生过量,造成细胞损伤,最终导致肾脏功能降低。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
Tropical Animal Health and Production -  相似文献   

15.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

16.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

17.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

18.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
  相似文献   

19.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

20.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号