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鸭病毒性肝炎Ａ６６鸡胚弱毒冻干疫苗的毒价为１０７．２４ＣＥＬＤ５０／０．１ｍｌ，冻干前同批鸡胚毒毒价为１０７．４０ＣＥＬＤ５０／０．１ｍｌ。冻干过程中损失的毒价在０．５个滴度之内。本冻干苗的免疫期与鸡胚混合毒相同，对雏鸡至少为４５天。冻干苗的有效保存期为：－２０℃，２年；０～４℃，８个月，２０～３０℃，１０天 相似文献
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鸡EDS—76油乳剂灭活苗的研制及免疫效果观察 总被引：1，自引：0，他引：1
以鸡ＥＤＳ－７６ＡＶ－１２７毒株为材料,研制出ＥＤＳ－７６油乳剂灭活疫苗,并对其物理性状、保存期、安全性及免疫效果进行了检测。结果表明：该苗的外观性状、物理稳定性、粘度以及安全性与免疫效果均已达到国内同类疫苗水平;用该疫苗免疫试验鸡,３０ｄＨＩ抗体可达９．６ｌｏｇ２,能很好地抵抗ＥＤＳ－７６ＡＶ－１２７强毒的人工感染;该苗于４℃保存期为１年,２０℃保存期为３个月。找出了油乳剂疫苗水相与油相的最佳合理配比、乳化时间、乳化速度及水相加入油相的速度。 相似文献
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为研究制定猪细小病毒病冻干疫苗的保存期,将3批疫苗分别在4℃保存3、6、9和12个月,在-20℃保存6、12、18和24个月,并进行物理性状、无菌检验、安全检验和效力检验,结果证实疫苗在4℃至少可保存12个月,-20℃至少可保存24个月。 相似文献
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实验室制备三批鸡疏螺旋体病鸡胚组织灭活苗，经效力检验，安全检验，免疫期及保存期测定结果表明，疫苗注射后体内抗体１５ｄ达最高水平，７ｄ和３０ｄ－７２０ｄ维持较高水平，免疫期达２年，疫苗保护率达１００５；疫苗在０－８℃条件下保存有效期为２年；疫苗安全性良好；三批疫苗试验结果不存在明显差异，显示制备工艺稳定。 相似文献
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利用禽脑脊髓炎（ＡＥ）琼扩抗原检测鸡血清中ＡＥ病毒抗体,对山东省４个地区８个种鸡群１２５份血清进行了检查,结果表明,山东种鸡禽脑脊髓炎感染率较高,鸡群感染率６３％（５／８）,检测鸡的感染率０～８７％之间,平均感染率３０％。 相似文献
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规模鸡场鸡脑脊髓炎血清学调查鸡脑脊髓炎（ＡＥ）是由禽脑脊髓炎病毒引起的一种主要侵害雏鸡的传染病。发病雏鸡出现运动失调，头颈震颤。成年鸡主要表现产蛋下降和生长缓慢。种蛋孵化率降低。为了解该病在规模鸡场的感染现状，笔者曾对２个规模场８个批次产蛋种鸡开展了... 相似文献
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鸡减蛋综合症蜂胶灭活疫苗的研究与应用 总被引：7，自引：0，他引：7
用纯化的天然免疫增强剂蜂胶研制成功鸡减蛋综合症蜂胶灭活疫苗。疫苗中蜂胶干物质含量为１０ｍｇ／ｍＬ。用该苗０．５ｍｌ免疫开产或临开产母鸡，接种后７ｄ ＨＩ抗体效为７￣１０（ｌｏｇ２），９０ｄ为９￣１２（ｌｏｇ２），１８０ｄ为８．７（ｌｏｇ２），３００￣３６０ｄ为６．５￣８．０（ｌｏｇ２）。免疫期为一年。－１５℃保存不冻结，保存期为１８个月，０￣８℃为１５个月，１０￣１５℃为１２个月，２５￣３０℃为３ 相似文献
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同群鸡两次出现脑脊髓炎发病高峰的报告马景星（山东省微山县畜牧兽医站·２７７６００）禽传染性脑脊髓炎（ＡＥ）是主要侵害幼雏神经系统的一种病毒性传染病，以发病雏出现两肢瘫痪，头颈震颤为主要特征．１９９３年１１月２０日至１２月２５日我县某鸡场饲养的６０００... 相似文献
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鸡新城疫CS2实验疫苗的生产及鉴定 总被引：1，自引：1，他引：0
应用ＳＰＦ鸡胚生产出３批ＣＳ２实验疫苗，疫苗的病毒价≥１０８．５ＥＬＤ５０／ｍｌ。对２月龄小鸡的最低免疫量（ＭＩＤ）为３～３０ＥＬＤ５０。据此，作者们建议对此种小鸡的实用免疫剂量为１０５ＥＬＤ５０。对这３批疫苗的实验结果显示：１０倍的剂量（即１０６ＥＬＤ５０）对鸡不引起任何不正常现象。免疫持续期达１２个月以上。在２～８℃和－１５℃中分别保存９和２４个月效力不变。 相似文献
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利用4型禽腺病毒HLJ1701株进行灭活疫苗的研制,并对疫苗的免疫效果进行评价,为家禽4型禽腺病毒的防控提供数据及参考。将HLJ1701株用灭菌生理盐水作10~4倍稀释后,接种9日龄SPF鸡胚,37℃孵育72 h后收获感染鸡胚尿囊液,经甲醛灭活后,加白油佐剂乳化制成油乳剂灭活疫苗,对制备疫苗的性状、安全性、免疫效力等进行检验。结果显示,制备的3批4型禽腺病毒灭活疫苗(HLJ1701株)均为油包水型,黏度均在50 cP以内,对3批疫苗取样,样品经3000 r/min离心15 min,管底无水相析出。安全性试验结果显示,将疫苗按1 mL/只超剂量接种3周龄SPF鸡,试验鸡在观察期内全部健活,未出现局部或全身不良反应,表明疫苗对SPF鸡具有良好的安全性;免疫效力及攻毒保护试验结果显示,用疫苗按0.2 mL/只的剂量免疫接种3周龄SPF鸡1次,免疫接种后21d试验鸡血清中HLJ1701株的抗体平均效价可达2~8以上,使用4型禽腺病毒(HLJ1701株)接种0.2 mL/只(100 LD_(50))对免疫鸡进行攻毒,疫苗对免疫鸡的保护率均为100%。研究表明,实验室条件下研制的4型禽腺病毒(HLJ1701株)灭活疫苗的各项指标均符合标准。 相似文献
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为了解云南省地方品种鸡群中禽白血病病毒(ALV)、禽网状内皮组织增生症病毒(REV)、鸡传染性贫血病毒(CAV)和禽脑脊髓炎病毒(AEV)的感染情况,采用ELISA方法对云南省滇东、滇西、滇中、滇南和滇北5个区域8个地方品种鸡群的2295份血清样品进行了上述4种垂直传播病毒的抗体检测。结果显示:云南省8个地方品种鸡群中,ALV、REV、CAV和AEV平均抗体阳性率分别为27.02%、72.77%、98.47%和76.25%,二重、三重、四重感染率分别为32.90%、36.38%、15.90%;不同区域、不同品种鸡群中均存在上述4种病原的感染,抗体阳性率有一定差异,但无规律性。结果表明,云南省地方品种鸡群中4种垂直传播病毒感染率较高,流行面较广,且呈现严重的混合感染状态。结果提示:应重视这4种可垂直传播病原的流行控制,谨防因病毒混合感染导致重大疫病免疫失败;继续加强抗体检测,及时淘汰阳性鸡群,净化种群,同时定期进行疫苗污染监测,提升饲养管理水平,保障云南省地方鸡种质资源的安全健康发展。 相似文献
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杭州动物园从北京动物园引进5对蓝鹇种源。日常食物主要是青菜、稻谷、家鸡用颗粒料、少量苹果。饲养过程中发生的主要疾病有细菌性肠炎、发情期的雄性打斗后的外伤等。杭州动物园经过精心饲养,3a繁殖达60多只。蓝鹇在杭州动物园的饲养和繁殖是全国人工饲养繁殖最好,且是全国最大人工种群。由于没有蓝鹇等野禽专用禽流感疫苗,杭州动物园使用家禽禽流感疫苗对蓝鹇进行禽流感免疫。根据家禽的禽流感免疫方法,每年对蓝鹇进行2次禽流感疫苗免疫,由于它的飞翔能力较强,每次免疫进行捕捉时均会乱飞,有时还会造成应激死亡,但最终免疫结果是否有效也未曾进行过检测。本研究中蓝鹇流感疫苗抗体检测采用的方法是：禽流感病毒H5抗体酶联免疫（ELISA）的检测方法。结果证明家禽流感疫苗对蓝鹇有效,经胸部肌肉注射0．5ml／次,每4个月肌注1次,每年免疫3次。雏鹇首免应在出壳后第8天、第9天或第10天。 相似文献
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An agar-gel-precipitin (AGP) antigen for avian encephalomyelitis virus (AEV) was prepared from infected chicken embryo brains. The antigen could precipitate specific antibodies to AEV. No nonspecific reactions were observed. Results of AGP tests were compared with those of virus-neutralization (VN) tests on both unvaccinated and AEV-vaccinated chickens. The AGP test reliably detected antibodies to AEV as early as four days postinoculation. Antibodies persisted in most vaccinated birds for over one year. 相似文献
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THE PATHOGENESIS OF INFECTIOUS AVIAN ENCEPHALOMYELITIS 总被引：1，自引：0，他引：1
An association was demonstrated between the development of clinical infectious avian encephalomyelitis (IAE), the persistence and titre of infectious avian encephalomyelitis virus (IAEV) in the brain of the chicken, the duration of detectable viraemia and the age of the chicken at the time of infection with the virus. The older the chicken at the time of infection the milder the disease, the lower the virus titre in the brain and the shorter the period of viraemia. IAEV serum neutralising antibody was produced earlier after infection in older chickens, and its detection was associated with decreasing virus titres in the brain and the cessation of detectable viraemia. Treatment of chickens with testosterone in ova, to inhibit the development of antibody synthesis, prevented the onset of age-associated resistance and testosterone treated birds were as susceptible to clinical IAE as baby chickens. The results suggested that the ability to produce IAEV serum neutralising antibody was an important component of age-associated resistance to IAE. 相似文献
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A commercial reovirus vaccine alone or experimental reovirus vaccine plus antibody complex were inoculated into 18-day-old specific pathogen free (SPF) broiler embryos at 0.1 of the recommended chick dose. The following groups were used: group 1A was not vaccinated or challenged; group 1B was not vaccinated, but was challenged with virulent reovirus; group 2 received the vaccine complexed with 1/4 dilution of antiserum; group 3 received the vaccine with 1/8 dilution of antiserum; group 4 received the vaccine with 1/16 dilution of antiserum, and group 5 received vaccine alone. At 1, 3, 6, 9, and 12 days of age, serum was collected and antibody against avian reovirus was analyzed by enzyme-linked immunosorbent assay (ELISA). At the same times, spleens were collected and vaccine virus detected by inoculating chicken embryo fibroblasts (CEFs) and examining for cytopathic effect. At 15 days of age, chickens in groups 2-5 were challenged with reovirus. At 22 days of age, birds were euthanatized and weighed. Efficacy of the vaccines was based on safety, percent protection, and antibody response. In ovo vaccination with the commercial or experimental vaccines did not adversely affect hatchability of SPF chickens. The vaccine complexed with antibody resulted in significantly less posthatch mortality (3.7%) when compared to mortality of chickens that received vaccine alone (17%). Both vaccine virus recovery and antibody response were delayed at least 3 days in birds receiving the experimental vaccines. In evo administration of reovirus antibody complex vaccines provided at least 70% protection. The experimental reovirus-antibody complex vaccines were safe and efficacious when given in ovo to SPF broiler embryos. 相似文献