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辣椒(Capsicum annuum L.)是我国第二大蔬菜作物,而雄性不育性状是辣椒杂交种选育中最重要的性状之一。该研究利用辣椒不育系3198A和恢复系3414-3-5的杂交后代F2分离群体249个植株,在观察花药形态的基础上,对花粉育性的分离情况进行了遗传分析。结果表明:不育系、恢复系、保持系三者的花药形态存在明显的差异,不育系的花药瘦小无花粉,恢复系、保持系的花药大而饱满,成熟花粉中充满淀粉粒;F2代花粉育性分离结果分析表明,辣椒雄性不育系3198A的雄性不育性状受到3对隐性核基因的控制,且存在光温敏不育基因。该研究结果将有助于进一步研究辣椒光温敏雄性不育的遗传特性,分离并选育出光温敏不育系,加速辣椒杂交种的推出。 相似文献
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通过对以8907A为不育源转育中育性的表现和育成的雄性不育系不育的稳定性观察,结果表明雄性不育的稳定性强烈地受到辣椒品种(自交系)遗传背景的影响,稳定不育的材料主要分布在早熟甜椒和牛角形、羊角形辣椒中,其他类型辣椒中较易出现育性不稳定的材料;通过8份不同类型的胞质雄性不育系自然杂交、人工杂交等采的杂种纯度鉴定表明,稳定不育的不育系在露地无论是自然杂交还是人工杂交杂种纯度均能保证100%,对于育性不稳定的材料,结合人工杂交,特别是隔离昆虫条件下人工辅助杂交,能显著提高杂种纯度. 相似文献
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辣椒恢复基因SSR标记定位及分子标记辅助选择育种 总被引：2，自引：0，他引：2
利用175对均匀分布在辣椒染色体上的SSR引物对辣椒不育系113A、恢复系139及其F2代群体进行筛选,发现AF208834引物和Rf基因连锁,遗传距离为20.8cM。用CRF-SCAR标记对36份恢复系材料进行分子标记辅助选择验证,结果表明,30份恢复系有特异条带,正确率为83.33%,并从108份未知育性材料中,检测到41份有恢复基因的材料;同时将SSR标记和SCAR标记结合起来,建立了与育性基因有关的多重PCR技术,进行辣椒恢复基因及其等位基因纯合状态的选择,显著提高了辣椒雄性不育三系的选择效率。 相似文献
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介绍了我国辣椒雄性不育研究在细胞学、内源激素、物质代谢等方面取得的新进展,分析了辣椒雄性不育在生产中的应用,并提出了展望. 相似文献
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利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST 总被引：4，自引：0，他引：4
以辣椒(Capsicum annuum L. ) 细胞质雄性不育系23A、121A, 和其相应的近等基因恢复系23C、121C为试验材料, 利用抑制消减杂交( SSH) 技术成功构建了CMS恢复基因诱导表达的消减cDNA文库。结合高密度点阵膜杂交差异筛选, 获得了282个阳性克隆。通过测序, 除去重复序列共得到175个Unique ESTs。在GenBank上进行BLAST分析, 55个EST片段未找到对应的同源序列, 可能代表了新基因;120个EST片段找到了对应的同源序列, 包括103个已知功能基因和17个未知功能基因。按照MIPS功能分类法, 将其分为14个功能组, 涉及代谢、胁迫应答、蛋白活性、转录因子、信号转导等多方面的功能。 相似文献
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M. S. Dhaliwal S. K. Jindal 《The Journal of Horticultural Science and Biotechnology》2013,88(5):471-479
SummaryPepper belongs to the genus Capsicum, of which five species are cultivated (C. annuum L., C. chinense Jacq., C. frutescens L., C. baccatum L. Ruiz. & Pavon, and C. pubescens). C. annuum L., which includes both hot pepper and bell pepper, is the most widely cultivated species. Commercial hybrid pepper seed is produced manually or by exploiting male sterility (MS). Both nuclear (NMS) and cytoplasmic (CMS) MS are available in pepper. Due to recessive gene control, the development of NMS lines through conventional breeding is tedious and time-consuming. Molecular markers linked to the NMS genes ms-1 and ms-8 have been developed. This will facilitate the development of new NMS lines, identify MS plants at the seedling stage, and test the purity of F1 hybrid seed. To reduce hybrid seed costs further, and to improve seed yields, there is a need to identify seedling markers to facilitate the removal of fertile plants in nursery blocks. Most reports have indicated that CMS is sensitive to low temperatures. Restorer-of-fertility genes are common in hot pepper and rare in bell pepper cultivars. Despite conflicting reports, it has been concluded that a single dominant gene (Rf) along with a modifier gene (Pr), controls the restoration of fertility in pepper. Molecular markers linked to the Rf and Pr genes and the mitochondrial CMS genes coxII and atp6 have been developed. These will facilitate the rapid screening of inbred pepper lines for Rf/rf alleles, accelerate the transfer of the rf gene in female hot pepper parents (maintainer breeding), and the Rf gene in male sweet pepper parents (restorer breeding). We have compiled up-to-date information on both NMS and CMS systems in pepper and discuss the prospects for their use in breeding for heterosis. 相似文献