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黄鳍东方鲀人工育苗技术初步研究 总被引：4，自引：0，他引：4
本文报道了福建省水产研究所2005年黄鳍东方鲀人工育苗技术研究结果.在水温18.6～20.2℃、盐度27.3～28.6条件下,亲鱼催产效应时间为39～49h;在水温18～21℃、盐度27.3～28.6条件下,受精卵孵化时间为120～216h;在水温18～21℃、盐度20.0～30.0条件下,室内水泥池前期培育13d,仔鱼成活率69.7%;在水温26.0～29.0℃、盐度15.0～30.0条件下,30万尾稚鱼经池塘50d后期培育,共培育出平均全长3.9cm、平均体重1.1g幼鱼5.0万尾,成活率16.7%. 相似文献
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黑脊倒刺鲃人工繁殖技术 总被引：7，自引：0，他引：7
采捕于溪河的野生黑脊倒刺Ba，在池塘生态条件下进行人工驯养使其达到性成熟。雌雄亲鱼的成熟率为83.8%和92.1%。采用HCG、LRH－A2等外源激素对成熟亲鱼进行人工催产。催产25组新鱼，获产18组。当水温为20－27℃时，效应时间15－23h，共得卵31500粒。平均催产率和受精率分别为72.0%和62.6%。受精卵在21－31℃水温下孵化，经52－110h仔鱼大量出膜，共孵仔鱼11947尾，育成夏花10956尾，成活率达91.7%。 相似文献
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催产亲鱼采自泉州湾双斑东方鲀产卵场海区，经注射LRH－A催产激素，3个试验组有2组产卵，共产卵61万粒；在水温15－18℃条件下，受精卵经4d孵化，仔鱼全部出膜，共出苗30万尾。 相似文献
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圆斑星鲽的人工繁殖及育苗技术 总被引：6，自引：0，他引：6
本文报道了圆斑星鲽的人工繁殖、育苗过程及结果，并比较了培育温度对仔、稚鱼生长和成活率的影响等。使用自然海区捕获的亲鱼，注射HCG，在水温14℃条件下，效应时间为24-28hrs。受精卵在水温14℃条件下，经94-96hrs孵出。仔鱼经55天培育，共培育出平均全长23．3mm的幼鱼5.5万尾，其成活率为18．3％。 相似文献
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褐毛鱨胚胎发育及仔、稚、幼鱼发育形态研究 总被引：1，自引：1，他引：1
褐毛鱨受精卵在水温25．8℃-26．5℃，盐度32的条件下，孵化时间为17h 30min，胚胎发育分为28期；在室内人工育苗，培育水温为23．8℃～26．2℃，盐度23-28，饵料依次为牡蛎幼虫、经营养强化的轮虫、卤虫幼体、桡足类的条件下，4日龄全长2．80mm进入仔鱼后期，18日龄全长10．50mm进入稚鱼期，25日龄全长23．00mm，全身鳞被完整进入幼鱼期。 相似文献
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鲟鳇杂交的人工繁育技术 总被引：1，自引：0，他引：1
在8～13℃水温下，对达氏鳇、史氏鲟亲鱼注射DOS（自行研制）、17α-羟基-20β双羟基孕酮（17α-20βp），在水温16～18℃时注射促黄体素释放激素类似物（LRH—A），流水刺激，诱导亲鱼排卵、排精。效应时间为8～18h，催产率100％、杂交受精率93．7％。采用全人工饲料进行开口试验和苗种培育试验，并获得85．7％开口成活率和77．33％的育苗成活率。生长曲线表明：不论是体长还是体重，50日龄前增长缓慢，50～100日龄则速度增长较快。经100d饲养，平均体长达29．5cm，平均体重达72．54g。 相似文献
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紫红笛鲷人工繁殖及育苗技术研究报告 总被引：2，自引：1，他引：2
利用海水土池或网箱养殖三龄以上的紫红笛鲷成鱼,于催产前三个月进行强化培育。在水温达到 20.0℃以上的春、夏季,用绒毛膜促性腺激素(HCG)和释放激素(LRH-A_(2-3))进行催产,混合或单一使用,剂量视亲鱼性腺成熟度而定。受精卵在盐度27.9-33.5,水温26.5-30.5℃,pH8.2的水环境中经15-17h孵化。开口仔鱼在土池水温27.2-35.5℃.盐度:23.5-35.6.pH8.0-9.5的条件下,经28-30d培育.鱼体全长为2.5-3.2cm,仔鱼变态完全,成为幼鱼。 相似文献
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川纹笛鲷人工育苗及生长的初步观察 总被引：1，自引：0，他引：1
本文报道了2003年川纹笛鲷人工育苗及成鱼饲养过程与结果。在水温25．4-31．4℃、盐度19．6～23．4条件下，川纹笛鲷仔鱼经36d培育，平均全长5．3cm，平均体重3．2g。在室内水泥池，川纹笛鲷经7个月饲养，平均全长为14．7cm，平均体重为113．7g。此外，对养殖期间水温不同阶段的生长进行了观察。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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