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1.
为探明2021年龙岩市规模化鸡场鸡传染性贫血病病毒(CIAV)、圆圈病毒3型(GyV3)、鸡细小病毒(ChPV)、J亚群禽白血病病毒(ALV-J)等4种病毒的感染情况,对龙岩市32个规模化鸡场采集的297份病死鸡组织样品进行PCR或RT-PCR检测,并对检测结果进行统计分析.结果显示:CIAV、GyV3、ChPV和AL...  相似文献   

2.
为了解福建省鸡场圆圈病毒3型（GyV3）的流行及其VP2基因变异情况,2021年在南平市、龙岩市和福州市的36个鸡场,采集365份疑似GyV3感染病鸡的腺胃样品,通过PCR进行GyV3核酸检测。结果显示：共检出GyV3核酸阳性55份,阳性检出率为15.06%。对5份GyV3阳性样品进行VP2基因序列分析发现,5份样品的VP2基因核苷酸和推导氨基酸序列相似性分别为98.6%～99.9%和98.3%～100%,与参考株山东肉鸡分离株SDAU-1和巴西家鸡分离株核苷酸和推导氨基酸序列的相似性分别为98.3%～99.2%和97.5%～99.2%,均属于Group A分支中的GyV3小分支。结果表明：福建省鸡场存在GyV3流行;其VP2基因遗传序列稳定,可作为GyV3感染流行病学监测的首选基因。本研究为GyV3的后续研究提供了理论依据。  相似文献   

3.
鸡传染性贫血病毒与其他禽免疫抑制性疾病病毒的混合感染   总被引：11，自引：2，他引：11  
应用多重PCR方法对广西主要养鸡地区的514只可疑鸡传染性贫血与其他禽免疫抑制性疾病临床病／死鸡进行了检测。结果显示，鸡传染性贫血病毒（CIAV）与其他禽免疫抑制性疾病病毒的二重或多重感染率达48．05％；CIAV阳性鸡群中，马立克氏病病毒（MDV）、网状内皮增生症病毒（REV）、禽白血病病毒（ALV）的群阳性率和个体阳性率均明显高于CIAV阴性鸡群，表明CIAV的感染与3种肿瘤病病毒的感染具有明显相关性，其中CIAV与MDV的感染具有明显相互促进作用；被调查鸡群中，传染性法氏囊病病毒（IBDV）和禽呼肠孤病毒（ARV）也有一定的感染率。结果表明,商业鸡群中各种免疫抑制性病毒的混合感染很普遍．与鸡群临床上所表现的生长阻滞、疫苗免疫效果不佳、总的发病率和死淘率增加等密切相关。  相似文献   

4.
为诊断2019年11月山东某规模化养鸡场商品肉鸡暴发腺胃炎的病因，本试验对病鸡进行临床、大体病变、组织病理和血涂片观察以及病原学检测。结果显示，发病鸡主要表现为生长停滞(矮小),精神沉郁，消瘦，消化不良等；大体病变表现为胸肌苍白，腺胃肿大，腺胃乳头消失，脾脏和法氏囊萎缩；组织病变表现为腺胃炎性细胞浸润，腺管上皮细胞增生，腺胃和十二指肠均有淋巴细胞浸润，可见核分裂象；血涂片中发现大量幼稚红细胞和异形性红细胞，并且异嗜性粒细胞增多；血琼脂培养基长出大小均匀一致的灰白色单一菌落；聚合酶链式反应(PCR)检测显示为大肠杆菌(E.coli);病毒PCR检测显示为网状内皮组织增生病病毒(REV)、鸡贫血病毒(CAV)。最终该病例诊断为REV、CAV、E.coli混合感染引起肉鸡腺胃炎。  相似文献   

5.
《畜牧与兽医》2015,(10):112-114
为了查明浦东某鸡场三黄肉鸡发生疑似鸡传染性贫血的病因,临床调查后采集相应病料,实验室研磨处理后备用。对病料进行鸡传染性贫血病毒(CIAV)的PCR检测、细菌分离鉴定及药敏试验。结果显示:3份病料接种鸡胚,收获的尿囊液PCR检测均为CIAV阳性,且细菌分离确定有大肠杆菌感染。药敏试验显示:分离的大肠杆菌对头孢噻呋、大观霉素敏感,对庆大霉素表现为中介,对阿米卡星、阿莫西林等13种药物均表现耐药。因此表明本次疫情为CIAV与大肠杆菌混合感染引起。  相似文献   

6.
为了解粤北地区鸡传染性病毒性腺胃炎（Transmissible viral proventriculitis,TVP）的病原学特征，于粤北地区8个有生长停滞、羽毛生长不良和消瘦等症状，疑似传染性病毒性腺胃炎的肉鸡场共采集样品420份，根据发病鸡场每10份样品混为1个样本，利用实时荧光定量PCR方法进行检测，8种TVP相关病毒的总检出率为80.95%(34/42),除呼肠孤病毒和传染性支气管炎病毒未检测到阳性外，鸡传染性贫血病毒和马立克氏病病毒感染率分别为57.14%、4.76%，传染性法氏囊病病毒、网状内皮增生症病毒、鸡腺胃坏死病毒和圆圈病毒3型均为2.38%，其中鸡传染性贫血病毒和马立克氏病病毒、马立克氏病病毒和传染性法氏囊病病毒等混合感染的检出率均为4.76%，马立克氏病病毒和鸡腺胃坏死病毒、马立克氏病病毒和圆圈病毒3型等混合感染的检出率为2.38%；发病鸡主要集中于25～80日龄，不同日龄的TVP相关病原学检出率无显著性差异。结果表明粤北地区肉鸡TVP发病率较高，需进一步完善相关疾病免疫策略，研究结果为进一步了解TVP发病规律和有效防控提供科学参考依据。  相似文献   

7.
将鸡传染性贫血病毒（Chicken infectious anemia virus,CIAV or CAV）和马立克氏病病毒（Marek s dis-ease virus,MDV）人工单一和共同感染1日龄的SPF鸡,感染后分别于14、21、28、35日龄检测鸡体红细胞压积的变化,并检测鸡群疫苗免疫3周后的抗体反应,以探讨CAV与MDV共感染对鸡体的免疫抑制是否有协同作用。结果表明,在血液分析方面,CAV与MDV共感染组较病毒单一感染组与对照组差异极显著,共感染不仅加重了鸡群贫血现象,而且延长了贫血的病理症状;而在禽流感病毒（Avian influenza virus,AIV）H5/H9疫苗、新城疫病毒（Newcastle disease virus,NDV）疫苗和传染性法氏囊病毒（Infectious bursal disease virus,IBDV）疫苗免疫后3周的抗体检测中,CAV与MDV共感染组较其它各实验组差异极显著,抗体滴度大大低于其它实验组;此外,CAV与MDV共感染组,鸡体生长状况明显差于实验各组,有6只鸡只死亡（6/25）,比病毒单一感染时的死亡率大大增加。综上研究证明,CAV与MDV共感染在免疫抑制作用上有协同作用。  相似文献   

8.
当前养鸡业中鸡传染性贫血病毒、禽网状内皮增生症病毒混合感染的发生率日益增高,为提高CIAV、REV检测效率,根据GenBank上登录的CIAV全基因序列和REV的LTR序列分别设计了2对引物,建立了REV、CIAV二重PCR检测方法,另外还比较了2种不同的DNA提取方法。结果显示,该方法扩增目的条带清晰、敏感性高、特异性好。应用建立的方法对临床7份病料样品检测,检出5份CIAV、REV阳性,表明建立的二重PCR方法能有效用于CIAV、REV混合感染的临床诊断。  相似文献   

9.
鸡J亚群禽白血病病毒与七种常见病毒混合感染的调查   总被引：1，自引：0，他引：1  
应用PCR方法对2009年6月到2009年9月来自不同地区、不同品种、不同日龄的45个疑似感染J亚群禽白血病病毒(ALV-J)的病鸡病料进行检测,同时对ALV-J与新城疫病毒(NDV)、传染性支气管炎病毒(IBV)、传染性法氏囊病病毒(IBDV)、H9N2亚型禽流感病毒(H9N2 AIV)、包涵体肝炎病毒(IB-HV)、鸡传染性贫血病毒(CIAV)和呼肠病毒(REOV)等病毒的混合感染情况进行了调查。结果表明,采用ALV-J的特异性引物H5/H7,在45份送检病料中,有3份检出ALV-J,阳性率为6.67%。绝大多数病例检测出内源性禽白血病病毒,部分病例检测有其他7种病毒中的一种或几种。混合感染病例中以IBHV和IBV混合感染的比率较高,其中ALV-J与H9N2亚型AIV混合感染多见。  相似文献   

10.
我国鸡传染性支气管炎病毒地方分离株生物学特性的研究   总被引：22，自引：3，他引：19  
从我国新疆维吾尔自治区某鸡场发病症状及病理变化疑似鸡传染性支气管炎的病鸡有出血点病变的腺胃组织中分离病毒，在9－11日龄SPF鸡胚上连续传代9次，通过病毒对鸡胚的致病作用、病毒在电镜下的形态观察、病毒在鸡胚中的增殖动态变化、病毒在CEF中的增殖特性、凝集鸡红细胞的特性以及动物回归试验等来研究我国鸡传染性支气管炎病毒地方流行株的生物学特性。结果表明，该毒株的第一代尿囊液对鸡胚无肉眼可见的致病作用，当继代到第5代后，胚体严重病变；电镜观察该病毒为典型的冠状病毒；病毒在鸡胚中随着接种病毒时间的延长，其效价增高，96小时可达到48小时的1倍，该毒株可在CEF上生长，但不能形成明显的蚀斑；并且经1％胰酶处理后可疑集鸡红细胞；鸡胚的第4代尿囊液病毒回归动物体，可致鸡病变病死鸡肾脏病变尤为明显，呈典型的花斑肾，腺胃则未见肉眼可见的病变，接种鸡、同居鸡和对照鸡之间NDV疫苗免疫后其HI抗体水平无明显差异，但从发病症状来看，IBV对ND疫苗具有干扰作用。  相似文献   

11.
Summary

Fourteen donkeys from a communal area of the Zimbabwean highveld were examined for stomach parasites during July and November of 1986 and January and April of 1987. All 14 animals were infected. Habronema muscae occurred in 12, Draschia megastoma in 11, H. majus in 9, Trichostrongylus axei in 9, Gasterophilus intestinalis in 14 and G. nasalis in 1 donkey. The lowest level of infection was in January for the helminthes and in April for Gasterophilus larvae. The peak burdens of parasites were in July for Habronema spp. and in November for D. megastoma and Gasterophilus larvae. Infections with spirurid worms and Gasterophilus develop mainly from the middle of the rainy season to the beginning of the dry season (Januari to May/June). G. intestinalis seems to have an annual cycle.  相似文献   

12.
Abstract

Extract

The results of the 1965–6 New Zealand national mastitis survey (Anon., 1967 Anon Ann. Rep. Res. Div. N.Z. Dept. Agric. 1966–67 1967 77 78  [Google Scholar]) indicated that β-haemolytic staphylococci were the most common bacteria isolated from the milk of dairy cows. Effective control of β-haemolytic stapyhlococci on a national scaledepends ultimately upon an understanding of population changes of this organism within the cow and within the herd.  相似文献   

13.
Effects of considering the comminution rate (kc) and the correction of microbial contamination (using 15N techniques) of particles in the rumen on estimates of ruminally undegraded fractions and their intestinal digestibility were examined generating composite samples (from rumen‐incubated residues) representative of the undegraded feed rumen outflow. The study used sunflower meal (SFM) and Italian ryegrass hay (RGH) and three rumen and duodenum cannulated wethers fed with a 40:60 RGH to concentrate diet (75 g DM/kgBW0.75). Transit studies up to the duodenum with Yb‐SFM and Eu‐RGH marked samples showed higher kc values (/h) in SFM than in RGH (0.577 vs. 0.0892, p = 0.034), whereas similar values occurred for the rumen passage rate (kp). Estimates of ruminally undegraded and intestinal digestibility of all tested fractions decreased when kc was considered and also applying microbial correction. Thus, microbial uncorrected kp‐based proportions of intestinal digested undegraded crude protein overestimated those corrected and kc?kp‐based by 39% in SFM (0.146 vs. 0.105) and 761% in RGH (0.373 vs. 0.0433). Results show that both kc and microbial contamination correction should be considered to obtain accurate in situ estimates in grasses, whereas in protein concentrates not considering kc is an important source of error.  相似文献   

14.
Abstract

Extract

Although exsheathment of the infective larvae of many strongylate nematode parasites of ruminants has been studied (Rogers, 1966 Rogers, W. P. 1966. “Exsheathment and hatching mechanisms in helminths”. In Biology of Parasites, Emphasis on Veterinary Parasites, Edited by: Soulsby, E. J. L. 33–39. New York and London: Academic Press. In [Google Scholar]), that of Cooperia curticei larvae has not. By analogy with other trichostrongylid species inhabiting the small intestine, they would be expected to exsheath in response to conditions provided by the abomasum, perhaps, as with Trichostrongylus colubriformis, with a potentiating effect of bile salts in the small intestine (Mapes, 1972 Mapes, C. J. 1972. Bile and bile salts and exsheathment of the intestinal nematodes Trichostrongylus colubriformis and Nematodirus battus. Int. J. Parasit., 2: 433–438.  [Google Scholar]). It was decided to see if C. curticei larvae exsheathed as expected; no attempt was made to define precisely the conditions necessary for exsheathment but rather to establish the site at which the stimulus is received.  相似文献   

15.
Summary By combined electron microscopical and serological methods the rate of involvement of pox virions within lesions of bovine natural dermatophilosis was determined. Scabby lesions of 40 (77%) out of a total of 52 cases were positive for the virions, while sera of all infected animals which reacted positively for pox viral antibodies (LSD) was significantly higher (P<0·001) in comparison to those of healthy-appearing animals. The virions in all cases had a uniform oval to cylindrical morphology with a mean measurement of 110 × 280 nm. A synergistic interplay involving the initiation of the scabby lesions by the virions and their subsequent trapping within keratin deposits, followed by their release through the keratolytic activity ofDermatophilus congolensis appear to have obtained in cases.
Frecuencia Del Compromiso De Viriones Pox En Lesiones De Dermatiofilosis Bovina
Resumen Se determinó la tasa del compromiso de viriones pox, con lesiones de dermatofilosis bovina natural, mediante la combinación de microscopía electrónica y serológica. Cuarenta lesiones costrosas (77%) de un total de 52 casos fueron positivas a viriones, mientras que el suero de todos los animales infectados que reaccionaron positivamente para anticuerpos pox (LSD) fueron significativamente más altos (P<0.001) en comparación a aquellos de animales aparentemente sanos. Los viriones en todos los casos tuvieron una morfología uniforme, de oval a cilíndrica con una media de 110 × 280 nm. Se obtuvo un sinergismo recíproco que involucró la iniciación de las lesiones costrosas por los viriones, los cuales quedaron atrapados subsecuentemente en los depósitos de queratina, seguido de la liberación de los mismos a través de la acción queratolítica delDermatophilus congolensis.
Frequence De La Participation De Virions Pox Dans Les Lesions De Dermatophilose Bovine
Résumé Par utilisation combinée de méthodes sérologiques et de microscopie électronique, on a pu déterminer la fréquence de participation de virions pox dans les lésions de dermatophilose bovine naturelle. Les lésions croûteuses de 40 (77%) cas sur 52 ont été positives pour les virions, tandis que les sérums de tous les animaux qui réagissent positivement pour les anticorps antiviraux pox (LSD) sont significativement supérieurs (P<0,001) comparés à ceux des animaux apparemment en bonne santé. Dans tous les cas, les virions ont une morphologie uniforme ovale à cylindrique, avec une taille moyenne de 110 × 280 nm. On a pu obtenir dans certains cas une interaction synergique, mettant en jeu l'initiation de lésions croûteuses par les virions et leur piégeage ultérieur dans les dépôts kératinisés, suivi de leur relargage dû à l'activité kératinolytique deDermatophilus congolensis.
  相似文献   

16.
Feedlot performance, carcass characteristics, and diet composition data were collected from a survey of finishing steer experiments (40 experiments; 347 kg average initial weight; data excluded Holstein steers). Data were analyzed by weighted (observations/mean) analyses of variance to determine effects of protein intake and implanting strategy on feedlot performance and carcass characteristics. Implanting strategies were defined according to prevalent or last implant type used: no implant (None); medium-potency implants (Medium): zeranol 72 mg per dose, steroid-based implants (Synovex-S or Compudose) or trenbolone acetate (TBA) alone; high-potency implants (High): TBA in combination with either steroids or zeranol. Regression procedures were utilized to estimate CP and DIP, or MP requirements. Implant effects were independent (P>0.60) of dietary protein effects and included faster (P<0.05) gains at higher intakes (P<0.05) that resulted in improved (P<0.05) feed efficiencies. Steers responded to higher dietary CP (13.3 vs 11.4%) by increasing intake (P<0.05) which resulted in faster (P<0.05) and more efficient (P=0.09) gains. Compared to nonimplanted steers, implanted steers had heavier (P<0.05) carcasses with larger (P<0.05) ribeyes and lower (P<0.05) marbling scores. Nonimplanted steers fed 13.3% CP diets had heavier (P<0.05) carcasses than nonimplanted steers fed 11.4% CP diets. Maintenance MP requirements of nonimplanted steers were greater than those of implanted steers and similar to established MP requirements. Diets of steers implanted with high-potency implants must be supplemented to contain more than 7.5 g MP/kg BW0.75/d, especially at heavy (>450 kg) initial BW, to maximize implant response. Implanted steers have a greater ability to respond to increased dietary protein because of reduced protein requirements for maintenance.  相似文献   

17.
Capromorelin is a ghrelin receptor agonist that is FDA approved for appetite stimulation in dogs. The objective of this study was to evaluate the safety of daily oral administration of capromorelin to cats over a range of doses and for an extended period. Two randomized, controlled studies were conducted: in Study 1, cats (n = 6 per group) received placebo or capromorelin at a dose of 9, 15, 30 or 60 mg/kg once daily for 14 days; and in Study 2, cats received capromorelin at 6 mg/kg (n = 8) or placebo (n = 4) once daily for 91 days. Cats were evaluated using clinical observations and clinical pathology test results for both studies, with the addition of postmortem examination in Study 1 and measurements of growth hormone and insulin‐like growth factor 1 in Study 2. Abnormal clinical observations were limited to emesis, hypersalivation, lethargy/depression, head shaking and lip smacking, which occurred more frequently in the capromorelin‐treated groups than in the placebo group. There were no clinically relevant differences in clinical pathology test results between the capromorelin and placebo groups in either study.  相似文献   

18.
Abstract

Extract

Sir — The recently published advances by Done (1957 Done, J. T. 1957. Vet. Rec., 69: 1341–1341.  [Google Scholar]). Done et al. (1957 Done, J. T., Brooksbank, N. H. and Buntain, D. 1957. Vet. Rec., 69: 67–67.  [Google Scholar]) and Harding cl al. (1957 Harding, T. D. J., Done, J. T. and Kershaw, G. F. 1957. Vet. Rec., 69: 824–824.  [Google Scholar]); ill Great Britain concerning the knowledge of different disease of the central nervous system ot pigs have prompted a closer examination of these diseases by Diagnostic Officers of the Animal Research Division in this country.  相似文献   

19.
Trials were conducted to evaluate the anthelmintic effect of a combination of epsiprantel at a dose rate of 5-5 mg/kg bodyweight and pyrantel pamoate at 5 mg pyrantel base/kg against Toxocara canis in prenatally infected unweaned greyhound pups, Toxascaris leonina, Uncinaria stenocephala, Trichuris vulpis and Dipylidium caninum in naturally infected adolescent greyhounds and Ancylostoma caninum, U stenocephala. Taenia hydatigena and Taenia pisiformis in artificially infected laboratory beagles. The product was well accepted and produced no obvious side effects. Percentage efficacy values based on post mortem worm counts were: T hydatigena 100; T pisiformis 100; D caninum 100; adult T canis 84-0; adult T leonina 96-5; immature T leonina 99-8; U stenocephala 99-0 and 87-7; A caninum 92-7; and T vulpis 43-3.  相似文献   

20.
Summary Precipitating antibodies against bluetongue were detected in sheep and goat serum samples collected from animals slaughtered in Baghdad abattoir. Out of294 sheep serum samples and110 goat serum samples examined,28 and18 samples respectively showed precipitating activity. In addition, examination of sheep serum samples collected from localities where clinical cases similar to bluetongue were previously reported revealed the presence of bluetongue precipitating antibodies in101 sera out of198 samples examined. This is the first report confirming the occurrence of blue-tongue in Iraq.
Evidencia Serológica De La Ocurrencia De Lengua Azul En Irak
Resumen Se detectaron anticuerpos precipitantes de la enfermedad de la Lengua Azul en el suero de ovejas y cabras beneficiadas en el matadero de-Bagdad. La prueba fue positiva en 28 de 294 sueros ovinos y en 18 de los 110 sueros caprinos examinados. Posteriormente, se examinaron 198 sueros ovinos colectados en áreas en donde se sospechaba la existencia clinica de la enfermedad, encontrandose 101 positivos. Este es el primer informe de la existencia de Lengua Azul en Irak.
Preuve Sérologique De L'existence De La Bluetongue En Irak
Résumé Des anticorps précipants contre la bluetongue ont été mis en évidence dans des échantillons de sérum de moutons et de chèvres recueillis sur des animaux abattus à l'abattoir de Bagdad. Sur 294 échantillons de sérum de moutons et 110 de chèvres examinés, 28 et 18 de ces échantillons se sont respectivement montrés précipitants. En outre l'examen d'échantillons de sérum de moutons recueillis dans des localités où existaient des cas cliniques pouvant faire soupçonner la bluetongue a montré la présence de sérums précipitants de la maladie dans 101 des 198 échantillons examinés. C'est là le premier report confirmant l'existence de la bluetongue en Irak.
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