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1.
Nematodes and flagellates are important bacterial predators in soil and sediments. Generally, these organisms are considered to be competitors for bacterial food. We studied the interaction among flagellates and nematodes using axenic liquid cultures amended with heat-killed bacteria as food and showed for the first time that a small and common soil flagellate (Cercomonas sp.) is able to attack and kill the much larger nematode Caenorhabditis elegans. The killing process is not caused by soluble metabolites but requires direct contact between the flagellate cells and the nematode surface and occurs rapidly (within a few hours) at high flagellate density. At lower flagellate density, adult nematodes sometimes avoid attachment of flagellates, feed on them and become the dominant bacterial predator. Considering that bacterial feeders affect bacterial communities differently, and that one bacterial feeder can control the abundance of another, suggests a new perspective on how bacterial diversity and trophic interactions are linked in the soil food web.  相似文献   

2.
The impact of protozoa on the availability of bacterial nitrogen to plants   总被引：9，自引：0，他引：9  
Summary Microbial N from 15N-labelled bacterial biomass was investigated in a microcosm experiment, in order to determine its availability to wheat plants. Sterilized soil was inoculated with either bacteria (Pseudomonas aeruginosa alone or with a suspension of a natural bacterial population from the soil) or bacteria and protozoa to examine the impact of protozoa. Plant biomass, plant N, soil inorganic N and bacterial and protozoan numbers were determined after 14 and 35 days of incubation. The protozoa reduced bacterial numbers in soil by a factor of 8, and higher contents of soil inorganic N were found in their presence. Plant uptake of N increased by 20010 in the presence of protozoa. Even though the total plant biomass production was not affected, the shoot: root ratios increased in the presence of protozoa, which is considered to indicate an improved plant nutrient supply. The presence of protozoa resulted in a 65010 increase in mineralization and uptake of bacterial 15N by plants. This effect was more pronounced than the protozoan effect on N derived from soil organic matter. It is concluded that grazing by protozoa strongly stimulates the mineralization and turnover of bacterial N. The mineralization of soil organic N was also shown to be promoted by protozoa.Communication No. 9 of the Dutch Programme on Soil Ecology of Arable Farming Systems  相似文献   

3.
Plant roots are densely colonized by bacteria which form the basis of the rhizosphere bacterial food web with protozoa as most effective predators. We established a well defined laboratory system with Arabidopsis thaliana as model plant allowing to investigate in detail the effect of rhizosphere interactions on plant performance. We used this system to analyse separate and combined effects of natural rhizobacteria and the protozoa Acanthamoeba castellanii on plants.Protozoa and bacteria increased plant growth with the effect of protozoa markedly exceeding that of bacteria only. Arabidopsis immediately responded to the presence of protozoa by increasing carbon but not nitrogen uptake. Later protozoa enhanced plant uptake of nitrogen from organic material and prolonged vegetative growth of Arabidopsis resulting in strongly increased seed production. It is concluded that the immediate plant response was based on changes in rhizosphere signalling inducing increased plant carbon fixation rather than on protozoa-mediated increase in nitrogen availability. The subsequently increased plant nitrogen uptake presumably originated from nitrogen fixed in bacterial biomass made available by protozoan grazing, i.e. the microbial loop in soil. The results suggest that Arabidopsis prepared for the upcoming mobilization of nitrogen by increasing carbon fixation and root carbon allocation which paid-off later by increased nutrient capture and strongly increased plant reproduction.  相似文献   

4.
We performed a controlled experiment with rice seedlings (Oryza sativa L.) growing in Petri dishes on homogeneous nutrient agar containing a simple rhizosphere food web consisting of a diverse bacterial community and a common soil protozoa, Acanthamoeba castellanii, as bacterial grazer. Presence of amoebae increased bacterial activity and significantly changed the community composition and spatial distribution of bacteria in the rhizosphere. In particular, Betaproteobacteria did benefit from protozoan grazing. We hypothesize that the changes in bacterial community composition affected the root architecture of rice plants. These effects on root architecture affect a fundamental aspect of plant productivity. Root systems in presence of protozoa were characterized by high numbers of elongated (L-type) laterals, those laterals that are a prerequisite for the construction of branched root systems. This was in sharp contrast to root system development in absence of protozoa, where high numbers of lateral root primordia and short (S-type) laterals occurred which did not grow out of the rhizosphere region of the axile root. As a consequence of nutrient release from grazed bacteria and changes in root architecture, the nitrogen content of rice shoots increased by 45% in presence of protozoa. Our study illustrates that interactions over three trophic levels, i.e. between plants, bacteria and protozoa significantly modify root architecture and nutrient uptake by plants.  相似文献   

5.
The capacity of bacteria and protozoa to mineralize soil nitrogen was studied in microcosms with sterilized soil with or without wheat plants. The effect of small additions of glucose or ammonium nitrate or both, twice a week was also tested. Plant dry weight and N-content, number of microorganisms and biomass plus inorganic N were determined after 6 weeks.The introduction of plants profoundly influenced the N transformations. In the presence of root-derived carbon, much more N was mineralized from the organic matter and immobilized mainly in plant biomass. “Total observable change in biomass N plus inorganic N” was negative in the unvegetated soils without additions, while a mineralization of 1.7 mg N microcosm?1 was observed in microcosms with wheat plants grown with bacteria only. When protozoa were included, the N taken up by plants increased by 75%. Sugar additions resulted in an 18% increase of total N in the shoots when protozoa were present, but had no significant effect in the absence of grazers. Plants with the same root weight were more efficient in their uptake of inorganic N when protozoa were present. Plants grown with protozoa also had a lower R/S ratio, indicating a less stressed N availability situation. The lowest ratio was found with N additions in the presence of protozoa.The results indicate that, with energy supplied by plant roots or with external glucose additions, soil bacteria can mineralize N from the soil organic matter to support their own growth. Grazing of the bacteria is necessary to make bacterial biomass N available for plant uptake.  相似文献   

6.
Aspalathus linearis is a commercially important plant species endemic to the Cape Floristic Region of South Africa and is used to produce a herbal tea known as rooibos tea. Symbiotic interactions between A. linearis and soil bacteria play an important role in the survival of Aspalathus plants in the highly nutrient-poor, acidic fynbos soil. The aim of this study was to characterize and compare rhizosphere and bulk soil bacterial communities associated with natural and commercially grown A. linearis, as well as the effect of seasonal changes on these communities. Bacterial communities were characterized using high throughput amplicon sequencing, and their correlations with soil chemical properties were investigated. The N-fixing bacterial community was characterized using terminal restriction fragment length polymorphism and real time quantitative polymerase chain reaction. Actinobacteria, Proteobacteria, and Acidobacteria were the most dominant bacterial phyla detected in this study. Highly similar bacterial communities were associated with natural and commercially grown plants. Significant differences in the bacterial community were observed between rhizosphere and bulk soils collected in the dry season, while no significant differences were detected in the wet season. This study provides insights into bacterial community structure and potential factors shaping bacterial community structure with commercially important A. linearis.  相似文献   

7.
Summary The biomass of microbial-feeding nematodes and protozoa was measured in the rhizospheres of peas, barley, grass and turnips grown for 10 weeks in pots containing a clay-loam soil; in the rhizospheres of peas and barley grown for 3 weeks in a sandy soil; and in the rhizosphere of barley grown for 11 weeks in an unfertilised and a fertilised clay-loam soil. The nematode biomass was consistently larger in the rhizosphere of all plants in both soils than in the bulk soil, but the protozoa biomass showed a rhizosphere effect only under pea and fertilised barley. The biomass of nematodes in the rhizosphere (1.2–22.3 g dry weight g-1 dry soil) was greater than the biomass of protozoa (0.1–3.2 g g-1), and greater under pea>barley>grass>turnip. It is suggested that nematodes are more able to exploit low bacterial densities than protozoa and that they initially migrate into the rhizosphere from the bulk soil. In samples of potato rhizosphere from field-grown plants, the nematode biomass was also greater than the active and total protozoan biomass. It is argued that in the rhizosphere the biomass of microbially feeding nematodes exceeds that of protozoa and that nematodes are more important in terms of nutrient cycling.  相似文献   

8.
Summary The impact of protozoan grazing on the dynamics and mineralization of 14C- and 15N-labelled soil organic material was investigated in a microcosm experiment. Sterilized soil was planted with wheat and either inoculated with bacteria alone or with bacteria and protozoa or with bacteria and a 1:10 diluted protozoan inoculum. 14C–CO2 formation was continuously monitored. It served as an indicator of microbial activity and the respiration of soil organic C. The activity of protozoa increased the turnover of 14C-labelled substrates compared to soil without protozoa. The accumulated 14C–CO2 evolved from the soils with protozoa was 36% and 53% higher for a 1:10 and for a 1:1 protozoan inoculum, respectively. Protozoa reduced the number of bacteria by a factor of 2. In the presence of protozoa, N uptake by plants increased by 9% and 17% for a 1:10 and a 1:1 protozoan inoculum, respectively. Both plant dry matter production and shoot: root ratios were higher in the presence of protozoa. The constant ratio of 15N: 14+15N in the plants for all treatments indicated that in the presence of protozoa more soil organic matter was mineralized. Bacteria and protozoa responded very rapidly to the addition of water to the microcosms. The rewetting response in terms of the 14C–CO2 respiration rate was significantly higher for 1 day in the absence and for 2 days in the presence of protozoa after the microcosms had been watered. It was concluded that protozoa improved the mineralization of N from soil organic matter by stimulating the turnover of bacterial biomass. Pulsed events like the addition of water seem to have a significant impact on the dynamics of food-chain reactions in soil in terms of C and N mineralization.Communication No. 19 of the Dutch Programme on Soil Ecology of Arable Farming Systems  相似文献   

9.
The effect of inoculation with Pseudomonas brassicacearum Am3, Pseudomonas marginalis Dp1 and Rhodococcus sp. Fp2 containing 1-aminocyclopropane-1-carboxylate deaminase (ACCD) on growth and uptake of N, P, K, Ca, S, Fe and Cd in shoots of pea (Pisum sativum) genotypes VIR188, VIR1658, VIR3429 and VIR4488 was studied in pot experiment with non-polluted and Cd-supplemented (10 mg Cd kg−1) sod-podzolic soil. The growth-promoting effect of bacteria depended on plant genotype and bacterial strain. Only Rhodococcus sp. Fp2 had no ACCD activity in vitro in the presence of Cd and did not stimulate pea growth in Cd-supplemented soil. Inoculation with bacteria counteracted the Cd-induced inhibition of nutrient uptake by plants probably through stimulation of root growth and enhancement of nutrient uptake processes. Nutritional effects of the bacteria were specific with respect to the nutrient.  相似文献   

10.
 Inoculation of the oilseed halophyte Salicornia bigelovii Torr. with eight species of halotolerant bacteria, grown in seawater-irrigated pots under environmental conditions native to the plant's habitat, resulted in significant plant growth promotion by the end of the growing season, 8–11 months later. Statistical analysis demonstrated that inoculation with Azospirillum halopraeferens, a mixture of two Azospirillum brasilense strains, a mixture of Vibrio aestuarianus and Vibrio proteolyticus, or a mixture of Bacillus licheniformis and Phyllobacterium sp. significantly increased plant height and dry weight at the end of the season. Some of the bacterial strains also increased the number of side branches and the size of the spikes. The bacteria did not affect the number of seeds or their weight. Inoculation with the mangrove cyanobacterium Microcoleus chthonoplastes had no effect on plant foliage variables. At the end of the growing season, the N and protein content of the plant foliage was significantly reduced by bacterial inoculation; however, the N and protein content of seeds significantly increased. The P content in foliage increased significantly in plants treated with all the bacteria except M. chthonoplastes, whereas the total lipid content of foliage increased significantly only when plants were inoculated with a mixture of A. brasilense strains or with M. chthonoplastes. In three inoculation treatments palmitic acid in seeds significantly increased and linoleic acid significantly decreased. This study demonstrates the feasibility of using bacteria to promote the growth of halotolerant plants cultivated for forage and seed production in proposed seawater-irrigated agriculture. Received: 3 January 2000  相似文献   

11.
The effects of bacterial-feeding nematodes on bacterial number, activity, and community composition were studied through a microcosm experiment using sterilized soil inoculated with soil bacteria （soil suspension） and with bacteria and three species of bacterial-feeding nematodes （ Cephalobus persegnis, Protorhabditis filiformis, and Caenorhabditis elegans）. Catalyzed reporter deposition-fluorescence in situ hybridization, CO2 evolution, and denaturing gradient gel electrophoresis （DGGE） of PCR ampli- fied 16S rRNA gene fragments were used to investigate bacterial numbers, antivity, and community composition, respectively. Our results showed that bacterial numbers and activity significantly increased in the presence of bacterial-feeding nematodes, which indicated that bacterial-feeding nematodes had a significant positive effect on soil bacteria. The different nematode species had different effects on bacterial numbers and activity. C. persegnis and P. filiformis, isolated from native soil, increased the bacterial number and activity more than C. elegans. The DGGE analysis results showed that dominant bacterial species significantly differed among the treatments, which suggested that bacterial-feeding nematode species modified the bacterial community composition in soil. Further gene sequence analysis results showed that the dominant bacterial species in this study were gram-negative bacteria. Given the completely same conditions except nematode species, the varied selective feeding behavior of different nematode species was the most likely reason for the altered bacterial community composition. Overall, the alteration of bacterial numbers, activity and community composition resulting from the bacterial-feeding nematodes may ult!mately affect soil ecological functioning and processes.  相似文献   

12.
Plant growth-promoting rhizobacteria (PGPR) naturally occur in the rhizospheres of pasture, but still little is understood regarding how soil agricultural practices affect them. Here, we examined the effects of long-term nitrogen (N) fertilisation on the occurrence of potential culturable PGPR in rhizosphere soils from pastures grown in Chilean Andisols. We also evaluated in vitro the effects of organic acids (citric, malic and oxalic acids), metals (Al and Mn) and N supply (urea and ammonium sulphate) on indole acetic acid (IAA) production and phosphorus (P) liberation by selected strains. Compared with non-N-fertilised pasture, N fertilisation significantly increased (30%) the occurrence of culturable phosphobacteria but decreased (7%) the occurrence of IAA-producing rhizobacteria. Most efficient IAA-producing phosphobacteria were identified as Bacillus, Enterobacter, Pseudomonas and Serratia. At low pH (4.8), the assays showed that the IAA production by Serratia sp. N0-10LB was increased (31–74%) by organic acids. On the other hand, the IAA production by Pseudomonas sp. N1-55PA was increased two- to fivefold by metals. In all strains, the growth and IAA production were significant decreased by 500 μM of Al, except Serratia sp. N0-10LB, suggesting its potential as PGPR for Chilean Andisols. When urea was added as main N source, the bacterial growth and P utilisation significantly increased compared with ammonium sulphate. The influence of environmental factors that are typical of Chilean Andisols on rhizobacterial communities will provide better management practices to enhance their PGPR functions as well as a better selection biofertilisers to be used in Chilean Andisols.  相似文献   

13.
Pea plants were grown in γ-irradiated soil in pots with and without addition of the AM fungus Glomus intraradices at sufficient N and limiting P. Depending on the growth phase of the plant presence of AM had negative or positive effect on rhizosphere activity. Before flowering during nutrient acquisition AM decreased rhizosphere respiration and number of protozoa but did not affect bacterial number suggesting top-down regulation of bacterial number by protozoan grazing. In contrast, during flowering and pod formation AM stimulated rhizosphere respiration and the negative effect on protozoa decreased. AM also affected the composition of the rhizosphere bacterial community as revealed from DNA analysis (DGGE). With or without mycorrhiza, rhizosphere respiration was P-limited on very young roots, not nutrient limited at more mature roots and C-limited at withering. This suggests changes in the rhizosphere community during plant growth also supported by changes in the bacteria (DGGE).  相似文献   

14.
The efficiency of 13 phosphate-solubilizing bacteria (PSB; four Burkholderia sp., five Enterobacter sp., and four Bradyrhizobium sp.) was assessed in a soil plate assay by evaluating soil phosphorus (P) availability. A commercial argentine strain, Pseudomonas fluorescens, was used for comparing solubilizing activity. Burkholderia sp. PER2F, Enterobacter sp. PER3G, and Bradyrhizobium sp. PER2H strains solubilized the largest quantities of P in the soil plate assay after 60 days as compared with the other strains, including the commercial one. The effect of PSB inoculation on growth and nutrient uptake of soybean plants was also studied under greenhouse conditions. Plants inoculated with Burkholderia sp. PER2F had the highest aerial height and showed an appropriate N/P ratio. However, none of the PSB increased P uptake by plants. This suggests that PSB inoculation does not necessarily improve P nutrition in soybean, nor was there any relationship between P availability in the soil plate assay and P content in the soybean shoot in the greenhouse. We concluded that the selection of efficient PSB strains as possible inoculation tools for P-deficient soils should focus on the integral interpretation of soil assays, greenhouse experiments, and field trials.  相似文献   

15.
为研究库布齐沙地生态恢复过程中不同植被恢复类型土壤微生物细菌群落结构、多样性的变化特征,以流动沙地为对照,运用高通量测序技术,对自然恢复的油蒿群落、人工种植的中间锦鸡儿群落根际和非根际土壤细菌多样性进行了研究,并分析了土壤理化性质对其分布的影响。结果表明:(1)与流沙对照相比,两种植被恢复类型对细菌多样性产生了正效应,细菌群落丰度、多样性和均匀度明显增加。其中,自然恢复的油蒿群落土壤细菌丰度高于人工种植的中间锦鸡儿群落;(2)变形菌门、酸杆菌门和放线菌门为研究区土壤中的优势细菌类群,其中变形菌门在各样地丰度比例最高,变形菌的4个亚群变化趋势一致,α-变形菌相对含量在油蒿和中间锦鸡儿群落根际土壤中明显增加,尤其是自然恢复的油蒿群落根际土壤中α-变形菌得到了很好的恢复;(3)土壤有机质、全氮、速效氮、速效钾含量和土壤含水量是影响土壤细菌群落丰度和多样性的主要土壤因子,典型相关分析表明土壤有机质、全氮、全钾、速效钾、速效氮含量对于研究区土壤细菌群落遗传多样性的变化起着重要作用。  相似文献   

16.
Multiple outbreaks of food poisoning associated with fresh vegetable consumptions have occurred in many countries. Numerous reports have described human pathogenic bacteria, such as Escherichia coli O157:H7 and Salmonella spp., that can internalize into fresh vegetables via root or leaf surfaces. While attempting to obtain the threshold concentration of internalization of E. coli inoculated into hydroponic medium during vegetable cultivation, we observed a rapid decrease in E. coli numbers. In the present study, we determined that the rapid decline in E. coli was not due to a physiological change into a viable but non-culturable (VNC) state. The population crash was instead caused by true bacterial death, as the rapid descent was also confirmed by micro-colony fluorescence in situ hybridization, a culture-independent method that can detect VNC cells. We next monitored the number of E. coli inoculated into intact or filter-sterilized hydroponic medium after cultivation of various types of plants. We found that the number of E. coli in intact hydroponic medium decreased markedly, whereas the level in filter-sterilized hydroponic medium was completely unchanged. This result suggests that biotic factors were present that could be eliminated by filtering. Robust predation of E. coli by protozoa (ciliates and flagellates) was observed using fluorescently labeled bacteria incorporated into the hydroponic medium. Finally, morphological identification of flagellates by scanning electron microscopy revealed the presence of a species of Stramenopiles. These findings suggest the importance of protozoa as bacterial feeders in hydroponic systems and hence the use of these organisms as potential control agents of human pathogenic bacteria.  相似文献   

17.
We studied the effect of bacteria involved in rock phosphate (four isolates), iron phosphate (two isolates), and aluminium phosphate (two isolates) solubilization, and two phytate-mineralizing bacteria in terms of their interaction with two Glomus spp. on Pueraria phaseoloides growth and nutrition. The plant —Rhizobium sp. — mucorrhiza symbiosis system may increase in yield and nutrition in association with specific rhizosphere bacteria that solubilize calcium, iron, and aluminium phosphates. No benefit from phytate-mineralizing bacteria was found under these experimental conditions. P. phaseloides growth responses were influenced in different ways by specific combinations of the selected bacteria and arbuscular mycorrhizal fungi. Considerable stimulation of nutrient uptake was observed with fungus-bacteria combinations of Azospirillum sp. 1, Bacillus sp. 1 or Enterobacter (spp. 1 or 2) associated with G. mosseae. The fact that Bacillus sp. 1, a calcium-phosphate solubilizing isolate, positively interacted with G. mosseae and negatively with G. fasciculatum is an indication of specific functional compatibility between the biotic components integrated in the system. From our results, the interactions between bacterial groups able to solubilize specific phosphate and mycorrhizal fungi cannot be interpreted as occurring only via P solubilization mechanisms since no generalized effect was obtained. Iron-phosphate solubilizing microorganisms were more active alone than in dual associations with Glomus sp., but the aluminium-phosphate dissolving isolates positively interacted in mycorrhizal plants. Further work is needed in this area in order to elucidate the mechanisms that affect rhizosphere microorganism interactions. G. mosseae was more effective but less infective than G. fasciculatum in most of the combined treatments.  相似文献   

18.
Bacteria and protozoa in soil microhabitats as affected by earthworms   总被引：8，自引：0，他引：8  
The effects of incorporation of elm leaves (Ulmus glabra) into an agricultural sandy loam soil by earthworms (Lumbricus festivus) on the bacterial and protozoan populations were investigated. Three model systems consisting of soil, soil with leaves, and soil with leaves and earthworms, respectively, were compared. The total, viable, and culturable number of bacteria, the metabolic potentials of bacterial populations, and the number of protozoa and nematodes were determined in soil size fractions. Significant differences between soil fractions were shown by all assays. The highest number of microorganisms was found in microaggregates of 2–53 μm and the lowest in the <0.2μm fraction. A major part of the bacteria in the latter fraction was viable, but non-culturable, while a relatively higher number of culturable bacteria was found in the macroaggregates. The number of colony-forming units and 5-cyano-2,3-ditolyl tetrazolim chloride (CTC)-reducing bacteria explained a major part of the variation in the number of protozoa. High protozoan activity and predation thus coincided with high bacterial activity. In soil with elm leaves, fungal growth is assumed to inhibit bacterial and protozoan activity. In soil with elm leaves and earthworms, earthworm activity led to increased culturability of bacteria, activity of protozoa, number of nematodes, changed metabolic potentials of the bacteria, and decreased differences in metabolic potentials between bacterial populations in the soil fractions. The effects of earthworms can be mediated by mechanical mixing of the soil constituents and incorporation of organic matter into the soil, but as the earthworms have only consumed a minor part of the soil, priming effects are believed partly to explain the increased microbial activity. Received: 7 January 1996  相似文献   

19.
Inoculants are of great importance in sustainable and/or organic agriculture. In the present study, plant growth of barley (Hordeum vulgare) has been studied in sterile soil inoculated with four plant growth-promoting bacteria and mineral fertilizers at three different soil bulk densities and in three harvests of plants. Three bacterial species were isolated from the rhizosphere of barley and wheat. These bacteria fixed N2, dissolved P and significantly increased growth of barley seedlings. Available phosphate in soil was significantly increased by seed inoculation of Bacillus M-13 and Bacillus RC01. Total culturable bacteria, fungi and P-solubilizing bacteria count increased with time. Data suggest that seed inoculation of barley with Bacillus RC01, Bacillus RC02, Bacillus RC03 and Bacillus M-13 increased root weight by 16.7, 12.5, 8.9 and 12.5% as compared to the control (without bacteria inoculation and mineral fertilizers) and shoot weight by 34.7, 34.7, 28.6 and 32.7%, respectively. Bacterial inoculation gave increases of 20.3–25.7% over the control as compared with 18.9 and 35.1% total biomass weight increases by P and NP application. The concentration of N and P in soil was decreased by increasing soil compaction. In contrast to macronutrients, the concentration of Fe, Cu and Mn was lower in plants grown in the loosest soil. Soil compaction induced a limitation in root and shoot growth that was reflected by a decrease in the microbial population and activity. Our results show that bacterial population was stimulated by the decrease in soil bulk density. The results suggest that the N2-fixing and P-solubilizing bacterial strains tested have a potential on plant growth activity of barley.  相似文献   

20.
Summary Soil texture affects pore space, and bacterial and protozoan populations in soil. In the present study we tested the hypothesis that bacteria are more protected from protozoan predation in fine-textured soils than in coarse-textured soils because they have a larger volume of protected pore space available to them. The experiment consisted of three sterilized Orthic Black Chernozemic soils (silty clay, clay loam, and sandy loam) inoculated with bacteria, two treatments (with and without protozoa), and five sampling dates. The soils were amended with glucose and mineral N on day 0. On day 4 bacterial numbers in all three soils were approximately 3×109 g–1 soil. The greatest reduction in bacteria due to protozoan grazing occurred between day 4 and day 7. Compared to the treatment without protozoa, bacteria in the treatment with protozoa were reduced by 68, 50, and 75% in the silty clay, clay loam, and sandy loam, respectively. On day 4, 2 days after the protozoan inoculation, all protozoa were active. The numbers were 10330, 4760, and 15 380 g–1 soil for the silty clay, clay loam, and sandy loam, respectively. Between day 4 and day 7, the period of greatest bacterial decline, total protozoa increased greatly to 150480, 96160, and 192100 g–1 soil for the three soils, respectively. Most protozoa encysted by day 7. In all soils the addition of protozoa significantly increased CO2–C evolution per g soil relative to the treatment without protozoa. Our results support the hypothesis that bacteria are more protected from protozoan predation in fine-textured soils than in coarse-textured soils.  相似文献   

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