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1.
The nitrate threshold concentration in rearing water of African catfish (Clarias gariepinus) was assessed. Female African catfish with an initial mean (SD) weight of 154.3 (7.5) g were exposed to 0.4 (Control), 1.5, 4.2, 9.7 and 27.0 mM nitrate for 42 days. Mean (SD) plasma concentrations of nitrate increased from 71 (29) to 6623 (921) μM at the highest ambient nitrate level. Mean (SD) plasma nitrite concentration ranged from 1.2 (0.5) to 7.9 (9.0) μM. Haematocrit, plasma concentrations of non‐esterified fatty acids (NEFA), cortisol, glucose, lactate, osmolality, gill morphology and branchial Na+/K+‐ATPase activity were not affected. Feed intake and specific growth rate were significantly reduced at the highest nitrate concentration. We advise not to exceed a water nitrate concentration of 10 mM (140 mg L?1 NO3‐N) to prevent the risk of reduced growth and feed intake in African catfish aquaculture.  相似文献   

2.
Cage‐pond integration system is a new model for enhancing productivity of pond aquaculture system. A field trial was conducted using African catfish (Clarias gariepinus) and Nile tilapia (Oreochromis niloticus) in cages and carps in earthen ponds. There were four treatments replicated five times: (1) carps in ponds without cage, (2) tilapia at 30 fish m?3 in cage and carps in open pond, (3) catfish at 100 fish m?3 in cage and carps in open pond, (4) tilapia and catfish at 30 and 100 fish m?3, respectively, in separate cages and carps in open pond. The carps were stocked at 1 fish m?2. The cage occupied about 3% of the pond area. The caged tilapia and catfish were fed and the control ponds were fertilized. Results showed that the combined extrapolated net yield was significantly higher (P < 0.05) in the catfish, tilapia and carps integration system (9.4 ± 1.6 t ha?1 year?1) than in the carp polyculture (3.3 ± 0.7 t ha?1 year?1). The net return from the tilapia and carps (6860 US$ ha?1 year?1) and catfish, tilapia and carps integration systems (6668 US$ ha?1 year?1) was significantly higher than in the carp polyculture (1709 US$ ha?1 year?1) (P < 0.05). This experiment demonstrated that the cage‐pond integration of African catfish and Nile tilapia with carps is the best technology to increase production; whereas integration of tilapia and carp for profitability.  相似文献   

3.
Ongoing research in recirculation aquaculture focuses on evaluating and improving the purification potential of different types of filters. Algal Turf Scrubber (ATS) are special as they combine sedimentation and biofiltration. An ATS was subjected to high nutrient loads of catfish effluent to examine the effect of total suspended solids (TSS), sludge accumulation and nutrient loading rate on total ammonia nitrogen (TAN), nitrite and nitrate removal. Nutrient removal rates were not affected at TSS concentration of up to 0.08 g L?1 (P > 0.05). TAN removal rate was higher (0.656 ± 0.088 g m?² day?1 TAN) in young biofilm than (0.302 ± 0.098 g m?² day?1 TAN) in mature biofilm at loading rates of 3.81 and 3.76 g m?² day?1 TAN (P < 0.05), respectively, which were considered close to maximum loading. TAN removal increased with TAN loading, which increased with hydraulic loading rate. There was no significant difference in removal rate for both nitrite and nitrate between young and mature biofilms (P > 0.05). The ATS ably removed nitrogen at high rates from catfish effluent at high loading rates. ATS‐based nitrogen removal exhibits high potential for use with high feed loads in intensive aquaculture.  相似文献   

4.
The nitrite toxicity was estimated in juveniles of L. vannamei. The 24, 48, 72 and 96 h LC50 of nitrite‐N on juveniles were 8.1, 7.9, 6.8 and 5.7 mg L?1 at 0.6 g L?1; 14.4, 9.6 8.3 and 7.0 mg L?1 at 1.0 g L?1; 19.4, 15.4, 13.4 and 12.4 mg L?1 at 2.0 g L?1 of salinity respectively. The tolerance of juveniles to nitrite decreased at 96 h of exposure by 18.6% and 54.0%, when salinity declined from 1.0 to 0.6 g L?1 and from 2.0 to 0.6 g L?1 respectively. The safe concentrations at salinities of 0.6, 1.0 and 2.0 g L?1 were 0.28, 0.35 and 0.62 mg L?1 nitrite‐N respectively. The relationship between LC50 (mg L?1), salinity (S) (g L?1) and exposure time (T) (h) was LC50 = 8.4688 + 5.6764S – 0.0762T for salinities from 0.6 to 2.0 g L?1 and for exposure times from 24 to 96 h; the relationship between survival (%) and nitrite‐N concentration (C) for salinity of 0.6–2.0 g L?1, nitrite‐N concentrations of 0–40 mg L?1 and exposure times from 0 to 96 h was as follows: survival (%) = 0.8442 + 0.1909S – 0.0038T – 0.0277C + 0.0008ST + 0.0001CT–0.0029SC, and the tentative equation for predicting the 96‐h LC50 to salinities from 0.6 to 35 g L?1 in L. vannamei juveniles (3.9–4.4 g) was 96‐h LC50 = 0.2127 S2 + 1.558S + 5.9868. For nitrite toxicity, it is shown that a small change in salinity of waters from 2.0 to 0.6 g L?1 is more critical for L. vannamei than when wider differences in salinity occur in brackish and marine waters (15–35 g L?1).  相似文献   

5.
We evaluated the effects of chloride concentration on the clinical pathology in juvenile Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus (Mitchill), following semi‐static exposures to 1 mg L?1 nitrite for 96 h. In spring water naturally low in chloride (5 mg L?1), plasma nitrite concentrated to more than 40× environmental levels resulting in a severe methaemoglobinemia characterized by torpid behaviour, 30‐fold increase in methaemoglobin fraction, anaemia, leucopenia and hyperkalaemia. Loss of intracellular water and potassium to extracellular space may have resulted in hyperkalaemia and haemodilution. Fish survived nitrite exposure, but 60% of torpid fish died following capture and tissue sampling. Fish acclimated to 10‐fold higher chloride content (55 mg L?1) did not concentrate nitrite in the plasma above environmental levels or develop methaemoglobinemia, but did exhibit similar haematology and plasma chemistry changes. Plasma nitrite returned to preexposure levels by 14 days following nitrite exposures, but severity of clinical pathology changes persisted or increased, suggesting that Atlantic sturgeon have reduced capacity to recover from methaemoglobinemia. Fish that survive methaemoglobinemia may be susceptible to mortality from the cumulative effects of intoxication, handling and other stresses for two or more weeks following nitrite remediation. Chloride buffering in aquaculture systems reduces the toxic effects of nitrite accumulation.  相似文献   

6.
A study was conducted to assess the effects of common carp and the African catfish on growth and reproduction of the native tilapia Oreochromis shiranus in Malawi. The study was done from 1 May to 1 October 2018 at the National Aquaculture Centre (NAC), Zomba. Four triplicated treatments were used: O. shiranus + carp (T1), O. shiranus + catfish (T2), O. shiranus + carp + catfish polyculture (T3) and O. shiranus monoculture (T4). Fish were stocked at uniform density of 0.8 fish/m2. Data collection was done once every month. Results showed that O. shiranus mean weight gain (%), specific growth rate (% body weight/day), average daily gain (g fish?1 day?1) and condition factor (g/cm3) were highest in T3 and lowest in T1 and T4 treatments. T3 had better water quality regime and higher tilapia biomass than T1 and T4 treatments. Tilapia fry production (no. fry pond?1 day?1) was highest in T4 but did not significantly differ (p > .05) between T2 and T3 treatments. It is concluded that the farming of common carp in aquatic ecosystems containing the African catfish may not adversely affect growth and reproduction of O. shiranus and that the polyculture of the African catfish, common carp and tilapia can be adopted to mitigate the potential adverse effects of carp on the environment and improve tilapia growth.  相似文献   

7.
The effects of ovine prolactin (oPRL) on osmoregulatory ability (electrolyte balance, plasma osmolality and activity of gill chloride cells and gill Na+/K+‐ATPase) and stress responses (plasma cortisol, glucose, aspartate aminotransferase: AST and alanine aminotransferase: ALT) were investigated in black porgy transferred to freshwater (FW). Fish in seawater (SW) were injected twice at a 24 h interval with oPRL (at 1, 3, or 5 μg g–1 body weight) or vehicle (0.9% NaCl) and then transferred to FW. They were sampled 3 days after the transfer. With oPRL at 5 μg g–1, levels of plasma Na+ and Cl? and osmolality were significantly higher than in saline‐treated fish, whereas gill CCs number and Na+/K+‐ATPase activity were lower. Also, the 5 μg g–1oPRL treatment led to significantly lower plasma cortisol levels than did saline treatment. However, there were no significant differences in plasma AST and ALT between groups. These results support the positive osmoregulatory role of PRL in black porgy during FW adaptation.  相似文献   

8.
We examined the effects of dietary supplementation for 21 days with different levels (0, 0.25 and 2.0 ml/kg) of the Aloysia triphylla essential oil (EOAT) on silver catfish (Rhamdia quelen). Cortisol levels were lower in fish fed EOAT at 2.0 ml/kg diet, and lactate levels were lower in those fed at both doses. Glucose, alanine aminotransferase, aspartate aminotransferase and triglyceride levels did not vary between groups. The biomarkers of oxidative stress, thiobarbituric acid‐reactive substances, lipid hydroperoxide, superoxide dismutase, catalase and non‐protein thiols, were lower in the brain, liver and muscle of fish fed EOAT at 2.0 ml EO/kg diet. Glutathione‐S‐transferase, reduced glutathione, plasma Na+, Cl?, and K+, and gill Na+/K+‐ATPase and H+‐ATPase did not vary between groups. Taken together, our results clearly indicate that the addition of EOAT at 2.0 ml/kg diet improves oxidative status and lowers the stress response in silver catfish.  相似文献   

9.
Two experimental modules with different stocking densities (M1 = 70 and M2 = 120 shrimp /m2) were examined weekly over a culture cycle in tanks with low‐salinity water (1.9 g/L) and zero water exchange. Results showed survival rates of 87.7 and 11.9% in M1 and M2, respectively. Water temperature, pH, dissolved oxygen, electrical conductivity and chlorophyll a were not significantly (p > .05) different between modules. In contrast, the concentrations of nitrogen compounds were significantly (p < .05) different between modules, except nitrite‐N (M2 were 2.31 ± 1.38 mg/L N‐TAN, 0.18 ± 0.49 mg/L N‐NO2? and 6.83 ± 6.52 mg/L N‐NO3?; in M1: 0.97 ± 0.73 mg/L N‐TAN, 0.05 ± 0.21 mg/L N‐NO2? and 0.63 ± 0.70 mg/L N‐NO3?). When waters of both modules reached higher levels of ammonia and nitrite, histological alterations were observed in gills. The histological alterations index (HAI) was higher in M2 (5‐112) than in M1 (2‐22).  相似文献   

10.
We used a 50‐year (1961–2010) daily record of precipitation and evaporation in a hydrological model to simulate ground water withdrawal for the foodfish grow‐out phase of ictalurid catfish culture in northwest Mississippi, USA. The model quantified the effects of seepage, reusing water for multiple years, and managing water levels to capture rainfall (drop‐fill water management). Selecting sites with relatively impervious soils and reusing water for multiple years had large impacts on annual water use, and combining those practices with drop‐fill water management reduced simulated groundwater withdrawal to less than 60 cm year?1 compared with more than 450 cm year?1 for the least conservative scenario. Water conservation measures reduced estimated costs of pumping ground water from ~$1150 ha?1 year?1 for the least conservative set of water‐use variables to less than $110 ha?1 year?1 for the best set of water conservation practices. Efficiency of pumped water use was dramatically improved by intensifying production in the foodfish grow‐out phase. Combining water‐conservation practices with production intensification improved the water use index from 9.18 m3 kg?1 for foodfish grow‐out ponds with the least conservative set of practices to 0.28 m3 kg?1 for ponds built on soils with negligible seepage, managed with a 22.9‐cm drop/7.6‐cm fill, drained every 10 years, and producing 15 000 kg of catfish ha?1 year?1. When simulated ground water use for the best set of water conservation practices in foodfish grow‐out ponds was combined with estimates of ground water used for fingerling production and water used in producing grain‐based feedstuffs, total consumptive water use index for catfish culture was estimated at ~2.7 m3 kg?1. This index is competitive with most other types of animal agriculture. Efficient water use in catfish farming is easily achieved under commercial conditions using existing simple technologies.  相似文献   

11.
This study examined ammonia, urea, creatinine, protein, nitrite, nitrate, and phosphorus (P) excretion at different water hardness, humic acid, or pH levels in silver catfish (Rhamdia quelen) juveniles. The fish were exposed to different levels of water hardness (4, 24, 50, or 100 mg L?1 CaCO3), humic acid (0, 2.5, or 5.0 mg L?1), or pH (5.0, 6.0, 7.0, 8.0, or 9.0) for 10 days. The overall measured nitrogen excretions were 88.1 % (244–423 μmol kg?1 h?1) for ammonia, 10.9 % (30–52 μmol kg?1 h?1) for creatinine, 0.02 % (0.05–0.08 μmol kg?1 h?1) for protein, 0.001 % (0.002–0.004 μmol kg?1 h?1) for urea, 0.5 % (0.64–3.6 μmol kg?1 h?1) for nitrite, and 0.5 % (0.0–6.9 μmol kg?1 h?1) for nitrate, and these proportions were not affected by water hardness or humic acid levels. The overall P excretion in R. quelen was 0.14–2.97 μmol kg?1 h?1. Ammonia excretion in R. quelen usually was significantly higher in the first 12 h after feeding, and no clear effect of water hardness, humic acid levels, and pH on this daily pattern of ammonia excretion could be observed. Water hardness only affected the ammonia and P excretion of R. quelen juveniles in the initial and fifth days after transfer, respectively. The exposure of this species to humic acid increased ammonia excretion after 10 days of exposure but did not affect P excretion. An increase in pH decreased ammonia and increased creatinine excretion but did not change P excretion in R. quelen. Therefore, when there is any change on humic acid levels or pH in the culture of this species, nitrogenous compounds must be monitored because their excretion rates are variable. On the other hand, P excretion rates determined in the present study are applicable to a wide range of fish culture conditions.  相似文献   

12.
Manipulation of stocking densities (10, 20, 30, 40 and 50 larvae L?1), each with or without the presence of shelter was conducted to determine the effects on survival, cannibalism and growth performances of larval bagrid catfish Mystus nemurus (Valenciennes 1840) from 2 to 14 days after hatching. This study revealed that stocking density significantly affected survival, cannibalism, total length, feed intake, specific growth rate and final weight of bagrid catfish larvae. Significantly higher survival was observed at moderate stocking density of more than 20 but less than 50 larvae L?1. Survival was significantly low beyond this threshold and was the lowest at 10 larvae L?1, coincides with the highest cannibalism. Total length, feed intake, specific growth rate and final weight were significantly higher at 10 larvae L?1. Shelter significantly improved total length and feed intake. No significant effects of stocking density and shelter were observed on the apparent feed conversion ratio and coefficient of variation. There was also no significant interaction between stocking density and shelter in all parameters. This study suggests that bagrid catfish larvae could be cultured at more than 20 but less than 50 larvae L?1 with the availability of shelter for optimal larviculture condition.  相似文献   

13.
Juvenile channel catfish (Ictalurus punctatus) were fed with nutritionally complete, basal diets supplemented with NaCl at 0, 10, 20 or 40 g kg?1 diet (0, 1, 2, or 4%) to apparent satiation twice daily for 10 weeks. Catfish were exposed to nitrite after six (7.70 mg L?1 nitrite‐N) and ten (7.18 mg L?1 nitrite‐N) weeks of feeding to determine the effect of dietary NaCl supplementation on resistance to nitrite toxicity. Fish were sampled before (baseline, pre‐exposure) and after 24‐h nitrite exposure to determine the effects of dietary NaCl on haematology (haematocrit, haemoglobin and methaemoglobin) and plasma electrolyte dynamics (nitrite, chloride, sodium and potassium). Mortality from nitrite toxicity was also determined. Mortality from nitrite exposure tended to decrease with increasing NaCl in the diet at 6 weeks and was significantly lower in the 40 g kg?1 NaCl group (12.5%) compared to the control group (57.5%). A similar trend in mortality occurred at 10 weeks as well; however, the differences among dietary treatments were not significant. The improvements in blood MetHb (non‐significant), chloride and nitrite levels in catfish may at least in part be responsible for the improved survival after nitrite exposure, which trended in support of the prevailing hypotheses for the positive effects of NaCl on nitrite toxicity.  相似文献   

14.
This study presents a simple urease method for analysis of ammonia and urea in freshwater aquaculture systems. Urea is hydrolysed into ammonia using urease followed by analysis of released ammonia using the salicylate‐hypochlorite method. The hydrolysis of urea is performed at room temperature and without addition of a buffer. A number of tests were performed on water samples obtained from a commercial rainbow trout farm to determine the optimal urease concentration and time for complete hydrolysis. One mL of water sample was spiked with 1.3 mL urea at three different concentrations: 50 μg L?1, 100 μg L?1 and 200 μg L?1 urea‐N. In addition, five concentrations of urease were tested, ranging from 0.1 U mL?1 to 4 U mL?1. Samples were hydrolysed for various time periods ranging from 5 to 120 min. A urease concentration of 0.4 UmL?1 and a hydrolysis period of 120 min gave the best results, with 99.6–101% recovery of urea‐N in samples spiked with 100 or 200 μg L?1 urea‐N. The level of accurate quantification of ammonia using the method is 50 μg L?1 NH4+‐N, and the detection level is 5–10 μg L?1 NH4+‐N.  相似文献   

15.
The anesthetic activities of the essential oils (EOs) of Hesperozygis ringens (EOHR) and Lippia alba (EOLA) and their effects in silver catfish (Rhamdia quelen) after anesthesia and recovery were investigated. Fish (32.19 ± 1.24 g) were submitted to one of the following treatments for each EO: basal group, control, or anesthesia (150, 300, or 450 μL L?1 EO). After that the anesthesia was induced or simulated and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. Fish were sampled at 0, 15, 30, 60, and 240 min after recovery. At time 0 of recovery, the ventilatory rate was lower in the groups anesthetized with either EO. In comparison with the basal group, control fish showed an increase in plasma glucose, aspartate aminotransferase (AST), and Na+ levels and a reduction in Na+/K+-ATPase activity at 0 min of recovery. Plasma levels of ammonia and Na+ were lower in the fish anesthetized with EOLA (450 μL L?1) and EOHR (all concentrations), respectively, than in the control fish. Additionally, lactate, AST, alanine aminotransferase, K+ plasma levels, and gill Na+/K+-ATPase and H+-ATPase activities were higher in the fish anesthetized with either EOHR or EOLA than in the control fish. The EOs promoted slight changes in silver catfish that enabled both an adaptive response and the recovery of most of the measured parameters after 240 min regardless of concentration or EO that was used. These findings support the use of EOHR and EOLA as anesthetics for fish.  相似文献   

16.
In vitro effect of the Melaleuca alternifolia, Lavandula angustifolia and Mentha piperita essential oils (EOs) against Ichthyophthirius multifiliis and in vivo effect of M. alternifolia for treating ichthyophthiriasis in one of the most important South American fish, Piaractus mesopotamicus (Holmberg), were evaluated. The in vitro test consisted of three EOs, each at concentrations of 57 μL L?1, 114 μL L ?1, 227 μL L?1 and 455 μL L ?1, which were assessed once an hour for 4 h in microtitre plates (96 wells). The in vitro results demonstrated that all tested EOs showed a cytotoxic effect against I. multifiliis compared to control groups (P < 0.05). The in vivo treatment for white spot disease was performed in a bath for 2 h day?1 for 5 days using the M. alternifolia EO (50 μL L ?1). In this study, 53.33% of the fish severely infected by I. multifiliis survived after the treatment with M. alternifolia (50 μL L ?1) and the parasitological analysis has shown an efficacy of nearly 100% in the skin and gills, while all the fish in the control group died. Furthermore, the potential positive effect of M. alternifolia EO against two emergent opportunistic bacteria in South America Edwardsiella tarda and Citrobacter freundii was discussed.  相似文献   

17.
This study was carried out to evaluate the antioxidative potential and quality of the meat of African catfish fed mulberry foliage extract (MFE). A total of 360 juvenile African catfish (8.4 ± 0.2 g) were fed four diets namely, basal diet (control), MFE‐2 (2 g MFE kg?1), MFE‐5 (5 g MFE kg?1) and MFE‐7 (7 g MFE kg?1) for 60 days. At the end of the experiment, muscles were excised, vacuum‐packaged and conditioned for 0, 7 and 14 days in a chiller (4°C). The meat from fish fed MFE‐5 and MFE‐7 had significantly (P < 0.05) greater total phenols content than the other dietary groups. This value reduced (P < 0.05) during storage. The DPPH‐scavenging effect of MFE‐7 increased (P < 0.05) compared with the others. It decreased (P < 0.05) during storage. The lowest POV was revealed (P < 0.05) in MFE‐7. The 2‐thiobarbituric acid‐reactive substances (TBARS) and peroxide value (POV) increased (P < 0.05) during storage. The pH value was significantly (P < 0.05) higher in MFE supplemented diets than in the control group. It is concluded that MFE at the concentration of 7 g kg?1 DM is potential dietary antioxidant supplements, to improve the quality of fish meat.  相似文献   

18.
Optimal water quality is considered as being a restriction for marine copepod cultures for live feed. There is a lack of knowledge on the water‐quality conditions in copepod cultures and the effect on copepods. Few studies have investigated the effect of ammonia on copepods, and fewer reports No Observed Effect Concentrations (NOEC) and Lowest Observed Effect Concentrations (LOEC), which provides safety levels before cultures are affected. This study investigates the tolerance of Acartia tonsa nauplii and adults to ammonia, using mortality as the endpoint after 24, 48 and 72 h of exposure. Nauplii were exposed to levels from 0 to 5127 μg NH3 L?1 and adults to levels from 0 to 8481 μg NH3 L?1. Nauplii NOEC was 30 μg NH3 L?1 and LOEC was 81 μg NH3 L?1. Adult NOEC was 477 μg NH3 L?1 and LOEC was 1789 μg NH3 L?1. 50% Lethal Concentrations (LC50) for nauplii of 48 and 72 h was 1257 and 220 μg NH3 L?1. LC50 for adults was 2370 (24 h), 972 (48 h) and 770 (72 h). Combining NOEC with excretion rates of NH4/NH3 a model was developed to calculate densities in batch cultures. We recommend that for batch cultures of A. tonsa, NH3 is kept below NOEC for nauplii and that levels of NH3 together with pH are monitored weekly.  相似文献   

19.
This study investigated the effects of the co‐supplementation of vitamins C (0, 500, and 1000 mg kg?1) and E (0, 62.5, and 125 mg kg?1) on the growth performance, haematology and the modulation of blood stress indicators and immune parameters in hybrid catfish (Clarias macrocephalus × Clarias gariepinus) under combinations of thermal and acidic stress. Supplementation of vitamins C and E influenced the growth, haematological indices, serum chloride, plasma protein and immune parameters (lysozyme, total immunoglobulin and alternative complement haemolytic assay) (P < 0.05). Although vitamins C and E did not prevent a significant reduction in serum chloride, they minimized not only the modulation of blood glucose and plasma protein, but also the reduction in immune parameters (P < 0.05) owing to stress. Our results demonstrated that co‐supplementation of 500 mg kg?1 vitamin C and 125 mg kg?1 vitamin E, or 1000 mg kg?1 vitamin C alone, for four weeks and co‐supplementation of both vitamins at low levels (vitamins C at 500 mg kg?1 and E at 62.5 mg kg?1) for eight weeks had beneficial effects on the growth, amelioration of stress‐mediated adverse changes in the physiological and immunosuppressive responses of hybrid catfish under stressful conditions.  相似文献   

20.
Microbial biomass estimates (based on ATP concentrations) were highly correlated with water and sediment respiration rates in freshwater prawn ponds. Water column biomass-specific respiration rates (x ± 1 SD, 572.4 ± 410.6 μl O2 μg ATP?1 day?1) were higher than sediment rates (11.5 ± 8.1 μl O2 μg ATP?1 day?1). Phytoplankton cells dominated the seston, with nutrient-saturated, light-limited growth in the range 75–80%μmax. Doubling times of phytoplankton ranged from 0.4–19 days. Measurement of benthic ATP biomass is recommended as a diagnostic water quality parameter for commercial freshwater prawn culture.  相似文献   

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