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1.
为研究欧拉羊在繁殖季节血清FSH和LH的含量与年龄、妊娠状态之间的相互关系．试验采用酶联免疫法（ELISA）测定妊娠期和非妊娠期欧拉羊血清套体促性腺激素的浓度。测定结果为非妊娠欧拉羊血清FSH浓度随年龄增长而增加,且6岁以上组显著高于2—3岁组（P〈0．05）。而LH的浓度在各年龄组间规律性变化;妊娠期欧拉羊2—3岁龄组血清LH含量显著大于其他两组（P〈0．05）。4—5岁年龄组血清FSH高于其他两组（P〈0．05）;就各个年龄阶段总体而言。非妊娠组的FSH和LH含量均显著高于妊娠组（P〈0．05）。得出妊娠状态是决定欧拉羊血清FSH和LH含量的关键因素。  相似文献   

2.
本研究采用放射性免疫分析法(RIA)测定了终生产公犊(n=3)、终生产母犊(n=3)的奶牛和普通奶牛(n=4)在排卵日和排卵后第10天血清中睾酮(T)、促卵泡素(FSH)、促黄体素(LH)、雌二醇(E2)的含量.结果显示,在排卵日终生产公犊奶牛血清中的FSH含量(2.42±0.35mIU/mL)均显著高于终生产母犊奶牛(0.96±0.50 mIU/mL,P＜0.05)和普通奶牛(1.30±0.14mIU/mL,P＜0.05)；终生产母犊奶牛血清中的T含量(1.20±0.12mIU/mL)显著低于终生产公犊(2.21±0.12mIU/mL,P＜0.05)和普通奶牛(1.98±0.04mIU/mL,P＜0.05),而3类奶牛之间的E2、LH含量差异不显著(P＞0.05)；在排卵后第10天,3类奶牛之间的T、FSH、LH和E2含量均无显著性差异(P＞0.05).奶牛后代性别决定与T和FSH含量有密切关系,排卵日FSH含量明显升高是终生产公犊奶牛的显著特征,排卵日T含量明显下降是终身产母犊奶牛的显著特征.  相似文献   

3.
选用 10匹 4～ 16岁怀孕和 2 2匹 0 5～ 17岁非怀孕的蒙古利亚矮种母马 ,采用放射免疫法分别测定了其血清中FSH和LH的浓度。其结果显示 ,妊娠母马的血清FSH和LH含量分别为 0 3 0 83mIU /ml± 0 1611mIU/ml和 0 65 67mIU /ml± 0 40 61mIU/ml,显著低于非妊娠母马的 2 40 5 9mIU/ml± 0 64 46mIU /ml和 2 3 85 5mIU /ml± 0 3 5 2 9mIU/ml(P <0 0 5 ) ;此外 ,3～ 8岁组、10～ 14岁组和 15岁以上组非妊娠母马血清FSH和LH含量分别为 2 44 0 0mIU /ml±0 780 0mIU /ml、2 40 0 0mIU/ml± 1 3 0 78mIU /ml、5 3 75 0mIU/ml± 0 885 0mIU /ml和 1 840 0mIU/ml± 0 80 0 0mIU /ml、2 5 92 9mIU/ml± 0 5 93 0mIU/ml、3 975 0mIU /ml± 0 485 0mIU/ml。以上结果表明 ,在妊娠阶段 ,母马血清中的FSH和LH显著降低 ,而繁殖季节的非妊娠母马血清FSH和LH的浓度却随着母马的老年化而呈现增加的趋势。  相似文献   

4.
奶牛血清中Zn、Fe与LH、FSH浓度的相关性分析   总被引：2，自引：0，他引：2  
本试验用原子吸收光谱法(AAS)和ELISA法分别测定了48头奶牛血清中Zn、Fe含量和促黄体激素(LH)、促卵泡激素(FSH)的浓度。结果表明,不同年龄奶牛血清Zn和Fe含量以7岁和5岁为最高;LH以4岁为最高(P0.01),FSH至4岁是最低(P0.01),然后升高;Zn与FSH、Fe与LH和FSH均呈负相关(P0.05);这些都和回归方程中截距A的正负号是完全一致的,同一种激素与不同元素回归方程的截距和回归系数均明显不同。可以得出不同年龄奶牛血清激素和微量元素含量变化较大,微量元素和激素间存在明显的相关性。  相似文献   

5.
对48只太行山羊(供体羊)、40只太行山羊和120只奶山羊(受体羊)进行了同期发情和超数排卵胚胎移植试验。其结果如下:(1)CIDR PMSG对太行山羊同期发情,有效发情率为75%;CIDR FSH对奶山羊同期发情,有效发情率为73%。(2)CIDR FSH LH组平均可用胚胎数分别与CIDR PMSG组和CIDR PMSG LH组之间差异达到极显著水平(P<0.01),而与CI-DR FSH组之间差异显著(P<0.05)。(3)CIDR FSH LH超排时,用CIDR FSH对奶山羊进行同期发情,受体妊娠率为56%;CIDR PMSG对太行山羊同期发情,受体妊娠率为60%水平。证明在非繁殖季节对太行山羊采用CIDR FSH LH超数排卵和以太行山羊及奶山羊为受体分别用CIDR PMSG和CIDR FSH同期发情来进行胚胎移植的技术方案是可行的。  相似文献   

6.
《畜牧与兽医》2016,(2):40-44
为研究自然发情和诱导发情的母犬发情期体内促卵泡素(FSH)、促黄体生成素(LH)、雌二醇(E2)和孕酮(P4)的分泌规律,将25只母犬随机分成2组:对照组为自然发情组,试验组为诱导发情组。对6只自然发情母犬和5只诱导发情母犬采血制备血浆,放射免疫分析法(RIA)测定FSH、LH、E2、P4的含量,采血时间为出现发情表征的第1、3、5、6、7、8、9、10、11、13、17、24天。对照组母犬FSH、LH和E2最低含量均值分别为(4.35±1.98)ng/m L、(4.95±2.22)ng/m L和(1.33±1.04)pg/m L,FSH、LH和E2峰值均值分别为(134.44±20.97)ng/m L、(117.56±17.49)ng/m L和(6.22±2.16)pg/m L,P4含量均值变化范围是(0.24±03)~(12.51±1.76)ng/m L;试验组母犬FSH、LH和E2最低含量均值分别为(4.19±2.12)ng/m L、(5.64±3.84)ng/m L和(1.57±0.76)pg/m L,FSH、LH和E2峰值均值分别为(172.98±56.40)ng/m L、(159.12±35.87)ng/m L和(6.08±1.16)pg/m L,P4含量均值变化范围是(0.24±0.06)~(12.51±3.95)ng/m L。结果表明:自然发情和诱导发情的母犬体内LH、FSH、E2和P4变化比较复杂,但仍有规律可循;E2对FSH的分泌具有一定的抑制作用,P4的含量变化在LH峰后的显著变化,P4稳定性和规律性更强;外源性PG-CI和FSH激素诱导发情的母犬的方法显著性提高了整个发情周期LH和FSH水平(P0.05),而对发情周期内E2和P4分泌水平均没有显著性影响(P0.05)。  相似文献   

7.
为了探讨饲粮中添加α-亚麻酸对母羊发情周期内生殖激素的影响,试验将体重相近、体况良好的2～3岁蒙古羊20只随机分为2组,对照组饲喂基础饲粮,试验组在基础饲粮中添加3%α-亚麻酸。试验期为50 d,其中预试期5 d、正试期45 d。在预试期5 d后给每只母羊肌内注射1 mL氯前列腺烯醇(PG),隔8 d再注射PG1 mL;6 d后给每只羊安装阴道栓,11 d后撤阴道栓并肌内注射孕马血清促性腺激素(PMSG)400 IU。每组随机选取6只母羊,在撤栓前1天及发情周期第1,10,20天采血,分离血清,并用放射免疫分析法测定血清雌激素(E2)、孕酮(P)、黄体素(LH)和促卵泡素(FSH)含量。结果表明：(1)与对照组相比,母羊血清E2、P、LH和FSH含量在撤栓前1天及发情周期第1,10,20天的4个时间点均显著提高(P<0.05)。(2)在发情周期内,母羊血清E2含量在发情初期最高(P<0.05),P含量在发情周期第10天左右进入高位(P<0.05),血清LH和FSH含量也是发情初期高、而后进入下降态势(P...  相似文献   

8.
本试验旨在研究补饲在青海牧区放牧状态下对无角型欧拉羊与有角型欧拉羊血液生化指标影响的比较。随机挑选健康、体况接近有角型和无角型的6月龄、12月龄和36月龄天然放牧欧拉羊各8只,共48只,分为2组(有角型为A组,无角型为B组),按年龄阶段营养需求补饲60 d,分别对有角无角欧拉羊进行空腹静脉采血取样。结果表明,补饲60 d后,在血清生化指标方面,6月龄B组白蛋白(ALB)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)含量高于A组,但差异不显著(P0.05),B组总蛋白(TP)、胰岛素(INS)含量显著高于A组(P0.05),12月龄B组谷丙转氨酶(ALT)含量显著高于A组(P0.05),36月龄A组甘油三酯(TG)、碱性磷酸酶(ALP)含量显著高于B组(P0.05);在血清免疫指标方面,6月龄B组免疫球蛋白M(IgM)、白细胞介素6(IL-6)含量显著高于A组(P0.05),而免疫球蛋白G(IgG)、白细胞介素2(IL-2)、白细胞介素4(IL-4)的含量也高于A组,但差异不显著(P0.05),12月龄B组免疫球蛋白M(IgM)、免疫球蛋白G(IgG)含量高于A组,但差异不显著(P0.05),36月龄B组免疫球蛋白M(IgM)、球蛋白(GLB)、白细胞介素2(IL-2)含量显著高于A组(P0.05);在抗氧化指标方面,6月龄和12月龄的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化酶(GSHPx)等含量均无显著性差异(P0.05),在36月龄时,A组丙二醛(MDA)含量高于B组(P0.05),但是6月龄和12月龄丙二醛(MDA)含量均未出现显著差异(P0.05)。上述结果表明,在放牧条件下,补饲可增强无角型欧拉羊的抗氧化能力和免疫功能,提高血清中生长激素的含量。  相似文献   

9.
采集4头体况良好、健康、配种后梅花鹿的血样,用RIA分析方法对血清中的5种生殖激素(LH、FSH、E2、P4和hCG)的含量进行测定.结果表明:在配种后(第2～38 d)LH含量缓慢地降低,处于较低的水平,平均为29.97±32.70 mIU/mL;FSH的含量稳步上升,平均为1.60±0.66 mIU/mL;E2的含量除了一个较大的峰值(10.20±6.59 pg/mL)外,一直在降低,平均值是6.47±4.28 pg/mL;P4的含量平均为0.05±0.04 ng/mL;hCG的含量呈缓慢下降趋势,平均为22.71±26.48 mIU/mL.  相似文献   

10.
为了在非繁殖季节判断绵羊的发情状况,试验以新疆高海拔地区中国美利奴羊为研究对象,采用酶联免疫测定(ELISA)法测定绵羊血清中雌二醇(E2)、孕酮(P4)、促卵泡素(FSH)、促黄体生成素(LH)的含量,研究激素诱导发情羊、发情症状不明显羊、不发情羊体内激素分泌规律的差异。结果表明:激素诱导发情羊的E2含量比不发情羊高,差异显著(P<0.05);但是在P4、FSH、LH水平上,激素诱导发情羊要低于不发情羊,差异不显著(P>0.05)。发情羊与发情不明显羊在4种激素含量上差异不显著(P>0.05),说明公羊试情存在一定缺陷,而通过测定E2含量判定绵羊发情状态将成为未来研究的重点。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
  相似文献   

17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

20.
Tropical Animal Health and Production -  相似文献   

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