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1.
SW  Lee  M  Najiah  W  Wendy  A  Zahrol  M  Nadirah 《中国农业科学(英文版)》2009,8(6):740-745
In this article, antibiogram and heavy metal resistance profile of bacteria isolated from giant freshwater prawn （Macrobrachium rosenbergii） hatchery in Malaysia are described. Although giant freshwater prawn was introduced into Malaysia since the 1980s, there was no database information on antibiogram and heavy metal resistance profile of bacteria from giant freshwater prawn （34. rosenbergii） hatchery in Malaysia. Therefore, this study was carried out to determine the effectiveness of antibiotic and heavy metal resistance profile to control bacterial diseases in M. rosenbergii hatchery. The results can provide valuable information for local M. rosenbergii post-larval producer. Antibiotic sensitivity test was carried out by disk-diffusion method against 15 types of antibiotics as follows： oxolinic acid （2 μg）, ampicillin （10μg）, erythromycin （15μg）, furazolidone （15 μg）, lincomycin （15μg）, amoxicillin （25 μg）, colistin sulphate （25 μg）, doxycycline （30μg）, florfenicol （30 μg）, flumequine （30 lag）, nalidixic acid （30μg）, tetracycline （30μg）, oleandomycin （15μg）, fosfomycin （50μg）, and spiramycin （100 μg）, whereas heavy metal resistance profile of the present bacterial isolates was determined by 2-fold agar dilution technique. In this study, 5 types of bacteria were successfully isolated; they were Aeromonas spp. （n = 77）, Escherichia coli （n = 73）, Edwardsiella spp. （n = 62）, Salmonella spp. （n = 75）, and Vibrio spp. （n = 43）. The result showed that furazolidone was the most effective antibiotic to control the bacteria isolated in this study, approximately 89.7% of the bacterial isolates were sensitive to this antibiotic. Multiple antibiotic resistance （MAR） index indicated that the hatchery water source and M. rosenbergii post-larval and sediment tanks were at high-risk exposure to the tested antibiotic. Furthermore, all the tested heavy metals （Cd2＋, Cr6＋, Hg2＋, and Cu2＋） failed to inhibit the growth of the bacterial isolates. Therefore, it indicated that the water source of the hatchery is contaminated with both antibiotic residues and heavy metal.  相似文献   

2.
A total of 241 Escherichia coli （E. coli） isolates from 349 avian samples （292 from cloacae, 29 from feed and water, 28 from dust and padding） were collected from Northeast, South, North, and Central China in recent years. The percentage of isolation was 69.1%. There are 67 serogroups each with 1-2 isolates distributed in different regions, and some of these regions had the preponderant serogroups. Antimicrobial-resistance （AR） of E. coli was so severe that the majority were multi-AR. Fifty percent strains were resistant to 10-19 antimicrobial drugs. Overall, the isolates represented resistance to nalidixic acid （88.1%）, tetracycline （85.7%）, sulfamethoxazole （81.0%）, trimethoprim-sulfamethpxazole （77.1%）, ampicillin （76.2%）, amoxilline （74.3%）, streplomycin （66.2%）, fluoroquinolones （57.1-66.7%）, chloramphenicol （52.9%）, gentamicin （39.0%）, and kanamycin （36.2%）. The isolates were sensitive to cefalexin, amoxilline-clavulanic acid, amikacin, and florfenicol with an AR rate of 0-19,5% only, The results showed that the AR was more severe in chicken farms in which the antibiotics were used broadly and repeatedly. This study indicated the AR characterization of E, coli in different areas of China. It will be a foundation for studying AR mechanism and regulating the usage of antimicrobial in the poultry industry.  相似文献   

3.
A total of 96 monoconidial Botrytis cinerea strains were isolated from strawberry field.Physiological characteristics and resistance analysis to boscalid were investigated.Most isolates showed resistant phenotype and the resistance frequency of highly resistant isolates was as high as 45.10%.The effective concentration that inhibited conidia germination by 50% relative to the control values for sensitive isolates ranged from 0.068 to 60.56 μg· mL~(-1) in conidia germination assays.Compared to the sensitive strains, the boscalid-resistant strains had a significant increase in phenylalanine ammonia lyase(PAL) and peroxidase(POD) activity and more sensitivity to boscalid pressure.Fitness analysis showed statistically significant difference between sensitive and resistance isolates.Sequencing of Sdh B gene indicated that a single mutation corresponded to His272.The results suggested that resistance to boscalid in B.cinerea populations could result in failure to control gray mold.These results would enrich the understanding of the resistance mechanism of B.cinerea to boscalid.  相似文献   

4.
The antibacterial activity of beta-lactam antibiotics or their combinations with inhibitor sulbactum against non-lactamase- producing strains, lactamase-producing and ESBLs-producing isolates was evaluated with twofold dilution method after pathogens isolated from pigs and chickens were detected, respectively, for beta-lactamase and extended-spectrum beta- lactamases （ESBLs）, The results revealed that most of 43 clinically isolated strains could produce beta-lactamase and 3 strains of shigella isolated from chicken samples produced ESBLs. All of 30 lactamase-producing strains isolated and only one of 16 non-lactamase-producing strains were resistant to amoxicillin and ampicillin. MICs of ampicillin against lactamaseproducing isolates decreased 10-40 and 10-20 times respectively, when it was conbined with sulbactam at ration of 1：2 and 1：4. All clinical isolates were susceptible to third-generation cephalosporins. The MICs of third-generation cephalosporins against lactamase-producing isolates did not change when they were conbined with sulbactam. MICs of ceftiofur and ceftriaxone against ESBLs-producing isolates decreased 2-4 times when they were conbined with sulbactam.  相似文献   

5.
Aeromonas hydrophila isolates from clinical cases （n=43） were tested against 8 antimicrobial agents and typed by outer membrane protein （OMP） pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin （MICs, ≥16 μg mL-1） and sulfamonomethoxine （MICs≥64 μg mLl）, but susceptible to norfloxacin （MICs,≤0.5 μg mL-1）. There was a high incidence of resistance to erythromycin （90.70%） and tylosin （93.02%）, while a low incidences of resistance to ciprofloxacin （2.33%）, enrofloxacin （2.33%） and florfenicol （4.65%）. Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, A.Pun and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.  相似文献   

6.
The pathogenicity of 36 isolates of Xanthomonas oryzae pv. oryzae （Xoo）, which were collected from japonica rice varieties in the Yunnan Plateau, China, was evaluated. It was evaluated on 29 rice varieties including a set of seven varieties to identify pathogenicity, i.e., Haonuoyang, TN1, Kogyoku, Zhenzhu＇ai, IR26, Nanjing 33, and Kinmaze, which may be considered as a set of differential varieties for Xoo races from Yunnan japonica rice. The efficiency of the seven varieties was further confirmed. The results showed reversible and specific interactions between isolates and varieties. The isolates were classified into nine pathotypes from pathotyp Ⅰ to Ⅸ according to their pathogenic reactions on the seven rice varieties. The pathotype V was the epidemic, whereas pathogen Ⅶ was the most pathogenic. Most japonica varieties grown in the Yunnan Plateau were susceptible to Xoo. The rice lines IRBB21 （Xa-21）, Zhachanglong （Xa-22,, Xa- 24,）, and IR1545-339 （xa-5）, which were resistant to all the isolates tested, can be used as donors of resistant genes for bacterial blight in japonica rice breeding in the Yunnan Plateau.  相似文献   

7.
The isolates of Phytophthora infestans on tomato in the Guangxi Zhuang Autonomous Region, China, were determined for the sensitivities to metalaxyl, cymoxanil and dimethomorph to give the basic information for integrating disease management. Sensitivities were tested by measuring the radial growth on agar medium amended with fungicide, compared with the floating-leaf-disk method. 239 isolates were collected from eight tomato growing areas during 2000-2006. The testing results indicated that the frequencies of sensitive, intermediate, and resistant isolates to metalaxyl were 42.26, 35.98, and 23.53%, respectively. Variations in sensitivities amongst isolates from different areas or different years were very high for metalaxyl. All isolates from Tianlin and Wuxuan were sensitive to metalaxyl, but the metalaxyl-resistant isolates predominated in Tianyang, with the frequency of 51.35%. The EC50 values of certain isolates from Tianyang were higher than 500 μg·mL^-1 and their resistance levels were over 100 000 folds. Cymoxanil has been used for nearly 10 years in Guangxi, and dimethomorph has been used for 5-6 years. However, there was no decrease in sensitivity of P. infestans populations and the sensitivities of the pathogen were nearly normally distributed. Hence, their mean ECs0 value [cymoxanil （0.1647 ±0.0255） μg·mL^-1, dimethomorph （0.0970 ± 0.0052） μg·mL^-1 could be used as the baseline sensitivities for monitoring the field resistance development. The comparison with the floating-leaf-disk method indicates that both the techniques provided equivalent results. These studies suggested that metalaxyl can be continuously applied in Tianlin, Wuxuan, and Nanning due to the resistant isolates that have not been found, while for those areas with resistant isolate, the use of metalaxyl should be reduced or alternated, and cymoxanil or dimethomorph was recommended for controlling late blight disease of tomato.  相似文献   

8.
The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution. Carbendazim has been widely used to control anthracnose caused by the C. gloeosporioides complex in China for more than 30 years and resistance to carbendazim has been reported in China. A total of 125 Colletotrichum isolates of strawberry and yam were collected from different geographical regions in Hubei Province, China. Approximately 52.8% of Colletotrichum spp. isolates showed resistance to carbendazim. The isolates tested in this study belong to four species, and the frequencies of resistant isolates differed across Colletotrichum species. Resistant isolates were found in C. siamense and C. fructicola. In contrast, all isolates of C. gloeosporioides and C. aenigma were sensitive to carbendazim. Highly carbendazim-resistant isolates harbored the E198 A mutation in the β-tubulin 2(TUB2) gene, whereas moderately carbendazim-resistant isolates harbored the F200 Y mutation in the TUB2 gene. Carbendazim-sensitive Colletotrichum isolates in this study were not genetically similar enough to form a separate cluster from resistant isolates. The result of this study emphasizes the importance of knowing which Colletotrichum sp. is present, when strategies for disease control are made.  相似文献   

9.
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10.
A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus (ERIC-PCR) method. The results showed that three different types, i.e. type Ⅰ, Ⅱ, and type Ⅲ, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed Ⅲ>Ⅰ>Ⅱ, while the isolates of Liaoning Province showed Ⅲ>Ⅱ>Ⅰ. The percentage of different types in different provinces varied in each other; however, they didn’t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.  相似文献   

11.
This paper compared antibiotic sensitivity between Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp (Litopenaeus vannamei). Recently, white leg shrimp become target species of shrimp culture among shrimp farmers in Malaysia to replace tiger shrimp (Penaeus monodon) culture. However, the baseline information on antibiogram of pathogenic bacteria especially Vibrio spp., the causative agent of vibriosis in white leg shrimp culture is not well established. Therefore, this study was conducted to reveal the antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp. The information gained from this study is useful for shrimp farmers in selecting appropriate antibiotic during disease outbreak. Antibiogram of present bacterial isolates was determined through disk diffusion method against 21 antibiotics (oxolinic acid 2 μg, ampicillin 10 μg, erythromycin 15 μg, furazolidone 15 μg, lincomycin 15 μg, oleandomycin 15 μg, amoxicillin 25 μg, colistin sulphate 25 μg, sulphamethoxazole 25 μg, chloramphenicol 30 μg, doxycycline 30 μg, florfenicol 30 μg, flumequine 30 μg, kanamycin 30 μg, nalidixic acid 30 μg, novobiocin 30 μg, oxytetracycline 30 μg, tetracycline 30 μg, nitrofurantoin 50 μg, fosfomycin 50 μg, and spiramycin 100 μg). A total of 47 Vibrio spp. isolates (Vibrio parahaemolyticus, 24, and V. alginolyticus, 23) from postlarval white leg shrimp and 49 Vibrio spp. isolates (Vibrio parahaemolyticus, 13, Vibrio alginolyticus, 28, and luminous Vibrio parahaemolyticus, 8) from marketable-sized white leg shrimp were successfully identified. Results of antibiotic sensitivity test from the present study showed that more than 80% Vibrio spp. from diseased postlarval and marketable-sized white leg shrimp, respectively, were sensitive to 14 out of the 21 tested antibiotics. Vibrio spp. isolated from marketable-sized white leg shrimp were found more susceptible to the tested antibiotics than Vibrio spp. isolated from postlarval white leg shrimp. This was due to 100% sensitive case against eight antibiotics found among Vibrio spp. isolated from marketable-sized white leg shrimp, whereas only three antibiotics were found sensitive to all Vibrio spp. isolated from postlarval white leg shrimp. Furthermore, the multiple antibiotic resistance (MAR) index indicated that marketable-sized white leg shrimp were not under high risk exposure to the tested antibiotics. On the other hand, the postlarvae were highly exposed to the tested antibiotics.  相似文献   

12.
This paper was the first report on pathogenic bacteria isolated from golden pompano (Trachinotus blochii) with the antibiogram, as well as the heavy metal resistance pattern. Golden pompano becomes popular among Malaysian fish farmers due to its high value and demand from local and oversea markets. However, the baseline information on antibiogram of pathogenic bacteria associated with golden pompano is not well established. Therefore, the information from this study may be useful to fish farmers in selecting appropriate antibiotic for treatment and prophylactive purpose in golden pompano culture. Isolation of bacterial isolates was carried out using 5% of Horse Blood agar, Tryptic Soy Agar (TSA), Mac Conkey, Thiosulphate Citrate Bile Salt (TCBS), Eosin Methylene Blue (EMB), Glutamate Starch Pseudomonas (GSP), Xylose Lysine Deoxycholate (XLD), and Baird Parker media. The bacterial isolates were then identified using conventional biochemical tests and confirmed by commercial bacterial identification kit. Antibiotic susceptibility test of bacterial isolates against 21 antibiotics (oxolinic acid 2 μg, ampicillin 10 μg, erythromycin 15 μg, furazolidone 15 μg, lincomycin 15 μg, oleandomycin 15 μg, amoxicillin 25 μg, colistin sulphate 25 μg, sulpha-methoxazole 25 μg, chloramphenicol 30 μg, doxycycline 30 μg, florfenicol 30 μg, flumequine 30 μg, kanamycin 30 μg, nalidixic acid 30 μg, novobiocin 30 μg, oxytetracycline 30 μg, tetracycline 30 μg, nitrofurantoin 50 μg, fosfomycin 50 μg, and spiramycin 100 μg) was determined using disk diffusion method, whereas the heavy metal resistance pattern (mercury Hg2+, cadmium Cd2+, chromium Cr6 +, and copper Cu2+) of the bacterial isolates was characterized using two-fold agar dilution method. Five bacterial species were successfully found from 50 diseased golden pompano. They were Streptococcus spp. (n = 12), Escherichia coli (n = 30), Salmonella spp. (n = 20), Pseudomonas spp. (n = 36), and Vibrio spp. (n = 50). More than 80% of the bacterial isolates were found sensitive to 11 out of 21 antibiotics (tetracycline, nitrofurantoin, florfenicol, chloramphenicol, oxytetracycline, nalidixic acid, doxycycline, furazolidone, flumequine, fosfomycin, and oxolinic acid. However, all bacterial isolates were found resistant to all tested heavy metals except for copper and cadmium. The multiple antibiotic resistance (MAR) index of the present study indicated that the fish samples were under high risk exposure to the tested antibiotics. Florfenicol is suggested to be used as antimicrobial agent for golden pompano culture since all bacterial isolates were sensitive to it.  相似文献   

13.
嗜水气单胞菌、爱德华氏菌培养物经甲醛灭活后,制成灭活油乳剂苗、灭活菌苗、饵料吸附型疫苗,免疫罗非鱼2周后,分别以嗜水气单胞菌、爱德华氏菌活菌液攻毒,结果显示,注射型疫苗均优于口服型,其中嗜水气单胞菌以腹腔注射0．1mL嗜水气单胞菌灭活菌苗＋0．3mL生理盐水的效果最佳,免疫保护率为85．0％;而爱德华氏菌以腹腔注射0．4mL灭活油乳剂苗效果最佳,免疫保护率为90．0％。两者的药物治疗试验结果显示,以氟苯尼考、恩诺沙星、环丙沙星、氟哌酸治疗罗非鱼嗜水气单胞菌病、爱德华氏菌病均有一定效果,对减少发病率及死亡率、控制病情具有较好作用,其中以氟苯尼考的疗效最佳,其用量为20mg／kgH2O。  相似文献   

14.
Antibiogram and heavy metal resistance patterns of pathogenic bacteria isolated from moribund cage cultured silver catfish (Pangasius sutchi) and red hybrid tilapia (Tilapia sp.) from Sungai Manir, Terengganu, Malaysia were studied and characterized. Sungai Manir is one of the famous rivers in Terengganu for its wide variety of cage cultured freshwater fish. However, to date, the baseline information of antibiogram and heavy metal resistance patterns of the pathogenic bacteria attacking the freshwater fish cultured in Sungai Manir is still lacking. Therefore, this study was carried out, which may be useful for fish farmers as a guideline for fish prophylactic and treatment purposes. Furthermore, present studies also provide information on the safety level of consuming freshwater fish produced from Sungai Manir. In the present study, bacteria were isolated from 100 fish of each moribund silver catfish and red hybrid tilapia using seven media including tryptic soy agar (TSA), Mac Conkey, thiosulphate citrate bile salt (TCBS), eosin methylene blue (EMB), glutamate starch pseudomonas (GSP), xylose lysine deoxycholate (XLD) and Baird Parker media. Identification of bacteria was carried out using conventional biochemical tests and confirmed by commercial bacterial identification kit. Antibiogram of the bacterial isolates against 18 antibiotics; oxolinic acid (2 μg), ampicillin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), oleandomycin (15 μg), amoxicillin (25 μg), colistin sulphate (25 μg), sulphamethoxazole (25 μg), chloramphenicol (30 μg), doxycycline (30 μg), florfenicol (30 μg), flumequine (30 μg), kanamycin (30 μg), nalidixic acid (30 μg), tetracycline (30 μg), nitrofurantoin (50 μg) and spiramycin (100 μg) was carried out using disk diffusion method, whereas heavy metal resistance patterns (Hg2+, Cd2+, Cr6 + and Cu2+) of the bacterial isolates was determined through twofold agar dilution method. The results showed that the percentage of sensitivity case of the 120 bacterial isolates to the tested antibiotics was 62.7%. This was followed by resistance (26.9%) and intermediary sensitive (10.4%) cases. In terms of the heavy metal resistance patterns, all bacterial isolates were resistant to Hg2+ and Cr6 +. However, only 27.8% and 16.7% of the bacterial isolates were sensitive to Cu2+ and Cd2+, respectively. The multiple antibiotic resistance (MAR) indices indicated that the cage cultured silver catfish and red hybrid tilapia were under high exposure to the tested antibiotic. Overall, the results of the present studies showed that Sungai Manir may be polluted with heavy metal and antibiotic residues.  相似文献   

15.
从疑似感染的成年散养鸡蛋壳和发病雏鸡的肝脏、脾脏中分离到2株细菌,通过培养特性、镜检结果、生化鉴定、血清学分型等,2株分离细菌均被鉴定为鼠伤寒沙门氏菌,分别命名为AH-DY01和AH-DY02。药物敏感性检测结果表明,AH-DY01对氟苯尼考、左氧氟沙星和环丙沙星敏感,对其他12种抗生素耐药;AH-DY02对阿米卡星、氟苯尼考、左氧氟沙星、复方新诺明、庆大霉素、链霉素、环丙沙星和大观霉素敏感,对其他7种药物耐药,2株沙门氏菌均表现多重耐药性。动物回归试验结果表明,2株鼠伤寒沙门氏菌均可致死雏鸡。对分离菌的16S r DNA进行测序与遗传进化分析,结果表明AH-DY01与标准菌株ATCC-1592和ATCC13311的同源性最高,而AH-DY02与日本分离株之间的亲缘关系最近。  相似文献   

16.
目前南美白对虾（Ｐｅｎａｅｕｓｖａｎｎａｍｅｉ）人工养殖中,病害多发,本研究从一养殖场中采集发病病虾,从患病对虾肝胰腺中分离病原菌．对分离菌株进行形态观察,生理生化测定并结合１６ＳｒＤＮＡ序列分析进行鉴定,结果表明,该分离菌为霍乱弧菌（Ｖｉｂｒｉｏｃｈｏｌｅｒａ）,回归感染证明其为致病菌,可导致南美白对虾死亡．通过纸片琼脂扩散法对３２种抗生素进行药物敏感试验,病原菌对氟苯尼考、氯霉素、利福平、诺氟沙星等１０种抗生素敏感,对头孢呋辛、头孢曲松等７种药物中度敏感,对羧苄西林、青霉素等１５种药物具有抗性．通过二倍稀释法测定筛选５种常用抗生素最小抑菌浓度,结果表明诺氟沙星抑菌效果最强,最小抑菌浓度最小＜０.２４４１μｇ／ｍＬ．  相似文献   

17.
为了解新疆焉耆县不同动物源沙门氏菌（Salmonella）对临床常用抗菌药物耐药和携带耐药基因的情况，以及不同动物源耐药沙门氏菌之间的亲缘关系，从新疆焉耆县几个规模化养殖场分别采集鸡、牛、猪及羊肛拭子样；对样品中分离出的沙门氏菌运用琼脂稀释法进行13种抗菌药物的最小抑菌浓度测定；并对耐药菌株通过PCR方法进行相关耐药基因的检测；采用MLST方法分析不同动物源耐药沙门氏菌之间的亲缘关系。结果显示：①分离鸡源沙门氏菌110株（27.5%；110/400），牛源沙门氏菌63株（64.9%；63/97），猪源沙门氏菌40株（10.0%；40/400），羊源沙门氏菌17株（6.8%；17/250）。②牛源沙门氏菌对氨苄西林、卡那霉素以及氟苯尼考这3种抗菌药物的耐药率均在50.0%以上，以7耐（33.3%）为主；猪源沙门氏菌对四环素的耐药率高于本地区其他动物源菌，多药耐药结果显示在0耐~7耐均有分布。③牛源沙门氏菌和羊源沙门氏菌中blaTEM、blaOXA、oqxA、oqxB、aac(6′)-Ib-cr、ant(3″)-Ia、aadA2和tetB基因的携带率高于鸡源和猪源沙门氏菌的携带率，但blaCMY-2、qnrB、qnrS和tetA这4种耐药基因仅在猪源沙门氏菌中检出。④筛选出8株多药耐药且携带多种耐药基因的沙门氏菌，MLST分析发现8株菌的ST型均为ST34，进化树分析显示它们之间存在较近的亲缘关系。结果表明，焉耆县被检养殖场的动物粪便中均分离出沙门氏菌，且对13种不同抗菌药物表现出不同程度的耐药，存在多种耐药基因共存的情况，不同动物源耐药沙门氏菌间存在克隆传播的机制。  相似文献   

18.
从患病海马Hippocampus kelloggi体内分离到3株菌株,用分离菌株的纯培养物感染健康海马,48 h内海马全部发病,其临床症状与原患病海马相似,经形态学、生化和室内感染试验,鉴定该菌株为副溶血弧菌Vibrio parahem olyticus。药敏试验结果表明,所分离菌株对链霉素、利福平、卡那霉素、环丙沙星、氟派酸、四环素、复达欣、菌必治、奈定酸等抗菌药表现为高敏感性;对红霉素、新霉素、先锋霉素Ⅵ、强力霉素、新生霉素、头孢呋肟、呋喃妥因等药物敏感性较高;对氨苄青霉素、羟苄青霉素、头孢氨苄、林可霉素等药物敏感性较差。  相似文献   

19.
A total of 241 Escherichia coli(E. coli)isolates from 349 avian samples(292 from cloacae,29 from feed and water,28 from dust and padding)were collected from Northeast,South,North,and Central China in recent years.The percentage of isolation was 69.1%.There are 67 serogroups each with 1-2 isolates distributed in different regions.and some of these regions had the preponderant serogroups.Antimicrobial-resistance(AR)of E. coli was so severe that the majority were multi-AR.Fifty percent strains were resistant to 10-19 antimicrobial drugs.Overall,the isolates represented resistance to nalidixic acid(88.1%),tetracycline(85.7%),sulfamethoxazole(81.0%),trimethoprim-sulfamethpxazole(77.1%),ampicillin (76.2%),amoxilline(74.3%),streplomycin(66.2%),fluoroquinolones(57.1-66.7%),chloramphenicol(52.9%),gentamicin (39.0%),and kanamycin(36.2%).The isolates were sensitive to cefalexin,amoxilline-clavulanic acid,amikacin,and florfenicol with all AR rate of 0-19.5%only.The results showed that the AR was more severe in chicken farms in which the antibiotics were used broadly and repeatedly.This study indicated the AR characterization of E. coli in different areas of China.It will be a foundation for studying AR mechanism and regulating the usage of antimicrobial in the poultry industry.  相似文献   

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