共查询到20条相似文献，搜索用时 436 毫秒
1.
2.
微波消解原子吸收法测定香菇中的矿质元素 总被引：1，自引：1，他引：0
采用微波消解样品,用火焰原子吸收分光光度法测定香菇中钾、铁、锌、铜含量。结果表明:在仪器工作条件下,金属离子含量与吸光度呈良好的线性关系,相对标准偏差0.27%~1.25%(n=6),回收率为93.7%~103.5%。该法具有简便、快速、损失低、污染少、实际利用率高的特点。 相似文献
3.
4.
超临界CO2萃取脱酯技术在香菇多糖提取中的应用 总被引：3，自引：1，他引：2
采用超临界CO2萃取脂酯技术从香菇中萃取油脂，对脱脂后的香菇粉采用浸提法提取香菇多糖，通过单因素及正交试验，得出最佳萃取条件为萃取压力30MPa，萃取温度40℃，CO2流量25L／h，萃取时间2h，总多糖提取率为6．57％。该方法与传统水浴浸提方法相比，提取率高出3．53％，为传统样的2．16倍．产品色泽和风味更接近标准品。 相似文献
5.
6.
7.
微波消解—原子荧光光谱法测定平菇和小棕蘑菇中总砷 总被引：1，自引：1，他引：0
采用微波消解-原子荧光光谱法,优化了微波消解-原子荧光光谱法测定食用菌中总砷的各种条件:前处理选用3.00 mL HNO3 1.50 mL30%H2O2作消解剂,采用方案3(步骤1维持时间5 min,步骤2维持时间12 min,其它参数参考MARS5微波消解植物样品推荐方案)消解样品,在400 mL/min载气流量,5 g/L硼氢化钾溶液还原条件下测定了平菇和小棕蘑菇中砷的含量,测定结果准确可靠,此方法的检出限为0.1 μg/L,标准偏差4.26%,试验回收率在86.4%～108.6%之间.该方法适用于原子荧光光谱法测定食用菌中的总砷. 相似文献
8.
9.
10.
11.
微波消解ICP-AES联用测定食用菌中锌、铁、锰和铜的含量 总被引：1，自引：0，他引：1
采用微波消解技术处理食用菌样品,用电感耦合等离子体原子发射光谱法(ICP-AES)同时测定锌、铁、锰、铜等微量元素的含量。实验结果表明:优化的消解剂用量为每0.25g食用菌子实体4.5mL HNO3+4.5mL H2O2;该方法适合测定食用菌中的锌、铁、锰、铜等微量元素,具简便、快速、稳定、准确等优点。方法的检出限为0.77～1.54μg/L,加标回收率为91.0%～101%,相对标准偏差为0.95%～7.10%。 相似文献
12.
以重庆市主城区普通厌氧消化污泥的干化处理产物样品为研究对象,通过盆栽试验,研究处理产物对花毛茛成活率、株高、冠幅、花径、地上部分生物质量等指标的影响。结果表明:使用普通厌氧消化污泥的干化处理产物处理的成活率偏低,处理产物对花毛茛幼苗的成活与生长有一定风险。第一次栽植15 d后对死亡的花毛茛进行补栽。补栽后,在小于等于33%的体积使用量范围内,使用处理产物对花毛茛的冠幅和地上部分生物量增长产生了一定的促进作用,其中的养分促进了花毛茛的生长;当使用量超过33%后,处理产物的使用对花毛茛生长表现出明显的抑制作用,这与高使用量下花毛茛成活率快速降低的情况一致。 相似文献
13.
AIM:To establish and compare the methods for culturing neonatal rat cardiac fibroblasts culture. METHODS:Neonatal rat hearts were isolated by collagenase+trypsin or trypsin digestion. The cardiomyocytes and cardiac fibroblasts were isolated by different attachment techniques. The cellular morphology, purity and reactions to the reagent were tested. RESULTS:For morphology and purity, no difference between the 2 methods was observed, though more myocytes were harvested by the method of collagenase+trypsin digestion. For the cellular responses to reagent, the cardiac fibroblasts harvested with trypsin digestion had more potent proliferative ability than those with collagenase+trypsin digestion. CONCLUSION:There is no difference of cellular morphology and purity in the cardiac fibroblasts isolated by collagenase+trypsin digestion and trypsin digestion, but the fibroblasts with trypsin digestion have more potent proliferative ability. 相似文献
14.
Biological characteristics of primarily cultured microvascular endothelial cells in mouse myocardium
AIM：To investigate an effective and stable method to isolate myocardial microvascular endothelial cells (MMVECs) from the mouse heart and to observe their biological characteristics.METHODS：The cells from the mouse heart were isolated by collagenase II digestion followed by different speed adhesion. Then the cells were cultured on the dish coated with polylysine in endothelial cell-specific culture medium. The biological characteristics were observed by trypan blue staining. The cell growth curve and the cell proliferation were also evaluated by MTT assay at different passages. The expression of DiI-ac-LDL, FITC-UEA-1, specific markers of endothelial cells and the expression of CD31, vWF and CD34 were determined by immunofluoresence staining. To evaluate the function of the MMVECs, in vitro tube formation was evaluated under microscope.RESULTS：Two days after the enzyme digestion, the MMVECs formed small and isolated clusters. The MMVECs grew quickly in monolayer with the characteristics of endothelial cell shape at 4~5 d. The cells became confluent and cobblestone-like which were ready for passage at 7~8 d. After passage, the viability of the cells was more than 95%. The first and the third generation of the cells presented an S-shape growth curve. The cell proliferation of the first to the third generation was quick and slowed down after the fifth passage. MMVECs were highly positive for DiI-ac-LDL and FITC-UEA-1 ［(89.2±3.5)%］, indicating the cells were MMVECs. The other relative antigen expression (CD31, vWF and CD34) on the MMVECs was (56.7±3.7)%, (78.5±2.6)% and (67.8±4.2)%, respectively. The MMVECs formed the tubes in vitro after cultured for 6～12 h.CONCLUSION：We can obtain high-purity MMVECs using the combination of collagenase II digestion, the different speed adhesion process and the endothelial cell-specific culture medium for effective and reliable MMVECs isolation and culture. 相似文献
15.
AIM: To establish a method for obtaining specific cells in solid tumor tissue by sorting of CD11b+ myeloid cells in hepatic metastases from colorectal cancer.METHODS: Tumor tissues were prepared into single cell suspension by mechanical method combined with enzyme digestion, and then the CD11b+ myeloid cells were isolated by flow cytometry. The sorted cells were identified by immunocytochemistry. The viability and morphologiy of the sorted cells were evaluated by Giemsa and Typan blue staining. The cell purity was evaluated by flow cytometry.RESULTS: Sufficient numbers of CD11b+ cells with high purity were isolated by sorting with flow cytometry from the single cell suspension prepared by mechanical and enzyme digestion. The purity of the cells was confirmed by statistical analysis (P<0.05). The positive rates of the cells before and after sorting were significantly different (P<0.01). The positive cells were verified by immunocytochemical method. Meanwhile, the sorted cells had complete morphology and good activity.CONCLUSION: The CD11b+ myeloid cells in solid tumor tissue can be isolated by flow cytometry from the machine-enzyme digestion suspension with high purity, good activity and complete morphology. 相似文献
16.
17.
18.
菊花品种资源遗传多样性的AFLP分析 总被引：13，自引：0，他引：13
以AFLP—银染分子标记技术, 对45个菊花品种进行了遗传多样性和亲缘关系分析。选用10个多态性高、分辨力强的E + 3 /M + 3引物组合分别对供试材料的基因组DNA进行扩增, 共获得486条清晰可辨的条带, 其中多态性带451条, 平均每个引物组合可检测出4511个多态性位点, 多态性位点百分率高达92.80% , 这表明供试品种资源在DNA水平上酶切位点的分布存在广泛的变异。应用DPS软件计算供试品种遗传距离界于0.36000～0.86237之间, 平均为0.611185; UPGMA分析将45份资源分成6个类群,从相异性系数分析了各品种资源间的亲缘关系。 相似文献
19.
CAO Kai-yuan ZHANG Tian XU Lin YUAN Guang-qing TIAN Xiao-dong HUANG Xiao-rong QIU Shao-peng 《园艺学报》2008,24(10):1877-1881
AIM: To study the blocking effect of shRNA on the expression of PSMA gene in LNCaP cell line by using shRNA eukaryotic expression vector. METHODS: Three pairs of DNA templates coding shRNA, synthesized against PSMA and cloned into the vector pSilencer 2.1-U6-neo, which was named pSilencer 2.1-U6-neo-shRNA, were identified by restriction endonuclease digestion analysis and DNA sequencing. LNCaP cells were then transfected with these three pSilencer 2.1-U6-neo-shRNAs and the negative control pSilencer 2.1-U6-neo-NC. After G418 selection, the cells were selected and the interfering effect was detected by RT-PCR and Western blotting. The biological behaviours of the transfected LNCaP cells were also tested. RESULTS: Restriction endonuclease digestion analysis and DNA sequencing results all showed that the 3 target segments were cloned into pSilencer 2.1-U6- neo vector respectively. After transfected into LNCaP cells, the inhibitory ratio of PSMA mRNA was 33.15%, 9.26% and 41.97% respectively, and that of PSMA protein was 26.26%, 6.47%, 40.69% respectively. The p-shRNA3 was chosen to test the cell growth and its invasive power in vitro. The results showed that after interfering, the invasiveness of LNCaP cells were enhanced. CONCLUSION: The vector-based shRNA on PSMA gene effectively knocks down the PSMA gene expression. The successful construction of PSMA shRNA makes it possible for further study of the interaction between PSMA and prostate cancer. 相似文献