共查询到19条相似文献，搜索用时 156 毫秒
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选择大鳞副泥鳅（Paramisgurnus dabryanus）进行人工采精,对精子进行保存,比较5种精子保存液（配方A、B、C、D、E）、5种糖及室温（20～24℃）和4℃对精子活力的影响,研究其精子保存的最佳条件,为规模化苗种的人工繁殖和杂交育种提供理论依据。结果显示,精子保存液保存的时间：C〉B〉A〉E〉D;精子保存液C、B、A、E、D分别能使精子在132h、132h、72h、22h、6h内使80％的精子保持快速运动的能力。在不同糖类进行比较的过程中,发现加入糖后的精子保存液比未加的保存效果要好,但不同糖类的效果又有差别,保存的时间为：葡萄糖〉蔗糖〉L-山梨糖〉D-果糖〉D-木糖。对20～24℃保存与低温4℃保存的效果研究表明,室温保存效果远不如冰箱低温保存效果。大鳞副泥鳅精子在淡水中快速运动时间能持续约31s,寿命约为130S。 相似文献
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黄颡鱼精子低温保存方法的初步研究及应用 总被引：1，自引：0，他引：1
以精子活力为标准,通过对4种保存液下黄颡鱼精子保存效果对比,筛选出保存效果较佳的基础保存液,并在此基础上对不同温度(0 ℃和4 ℃)、不同青霉素浓度和添加抗冻剂与否的条件下保存液保存效果做对比研究.试验结果表明,渔用任氏液保存效果较佳,提高青霉素浓度(通常为0.5×104 ～1.0×104 IU /ml,使用2.0×104 IU /ml),添加抗冻剂(6%DMSO),在添加抗冻剂条件下降低温度(由4 ℃降为 0 ℃)均可明显提高保存效果,在最优条件(0 ℃、2.0×104 IU青霉素/ml、6%DMSO)下,12 d内能保持80%的精子活性.人工授精试验证明了经保存的精子能正常用于人工繁殖,且授精效果与新鲜精液精子无显著差异. 相似文献
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南方鲇精子保存方法的初步研究 总被引：6，自引：0，他引：6
以精子被激活后的活力为指标，对6种精子保存液保存南方鲇的效果进行了评估，并对室温（18－20℃）和低温（0－4℃)状态下保存南方鲇精子的效果进行了比较研究。结果表明，在6种精子保存液中，以添加牛血蛋白和氨基酸等营养物质的F精子保存液效果最好，精子的活力和寿命保持时间最长，6d内可保存80％以上活力，寿命持续12.5d。低温下保存效果绝对优于室温下保存。 相似文献
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黑龙江茴鱼(Thymallus arcticus grubei Dybowski)精子活力的观察 总被引：5，自引：2，他引：3
用BSS和水两种激活剂对黑龙江茴鱼精子活力进行测定,并在4℃和14℃(室温)条件下,对精原液和用ASP稀释的精液分别进行保存试验.试验结果显示,用BSS和水均可激活黑龙江茴鱼精子,其精子活率分别可达100%和98%.14℃条件下,用BSS激活黑龙江茴鱼精子平均寿命为72.1s,A级(快速)运动时间平均为14.1s;用水激活精子平均寿命为67.2s,A级运动时间平均为12.5s.不同温度保存的精子寿命和快速运动时间也有显著差异:精原液4℃保存2h后精子失去A级运动,其激活率在70%以上,保存6h后激活率小于20%;精原液14℃保存1.5h后精子失去A级运动,其激活率大于70%,4h后激活率小于20%.经ASP(pH分别为7.0、7.8、8.0、8.5、9.0)稀释的精液,在4℃和14℃条件下,BSS和water对精子的激活率均小于20%,且精子均呈现C、D级运动,1.5h后各保存组激活率为0.试验结果表明,BSS对黑龙江茴鱼的精子有良好的激活效果;精原液4℃保存效果好于14℃,在4℃保存条件下2h以内使用,可以得到理想的结果;精液不宜用ASP稀释保存. 相似文献
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试验结果表明,虾夷扇贝精子的最适盐度为23.2～32,最适pH 7.0～8.5.4℃,未稀释精液样品保存5 d后,精子仍有(26±4)%的活力,而经海水稀释后的精液保存70 h后,最高活力仅为(11±1.41)%,用抗冻液稀释的精液,4℃保存5 h后,得到最高的精子活力仅为(24.7±4.16)%.添加抗冻剂后,-18℃的保存效果明显低于4℃保存的未稀释样品.用4℃保存的未稀释精液与栉孔扇贝卵子进行杂交试验,保存3 d内的精子获得的授精率与对照组差异不显著(P0.05).杂交组的幼虫可以正常发育至D形幼虫,与自交组无区别. 相似文献
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对瓦氏黄颡鱼(Pelteobagrus vachelli)精子在不同盐度和pH下的精子活力进行观察,同时研究了精子在4种不同稀释液与2种不同浓度抗冻剂组成的保存液中的超低温冷冻保存,并开展了冻精的授精实验。结果表明,瓦氏黄颡鱼精子浓度为(2.035±0.179)×1012cell·mL-1,在盐度为5.8、pH为7.17时,精子的活力都高达95%。以A液作为稀释液、10%甲醇作为抗冻剂时,冷冻保存精子效果最好,解冻后精子活力为(81.7±0.9)%。用解冻后的精子进行人工授精,获得的受精率为(88.4±2.1)%,孵化率为(74.0±0.8)%;而鲜精受精率为(91.0±0.8)%,孵化率(82±1.6)%,冻精与鲜精均无显著性差异。人工授精实验证明了解冻后的精子能正常用于该鱼的人工繁殖。 相似文献
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This study tested KUROKURA solution (Kurokura et al., 1984, Aquaculture 37, 267–274) and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of potential motility of tench spermatozoa. The immobilizing solution is used because, when collected, the sperm of most samples is contaminated by urine, causing spermatozoa to be of poor quality, with low motilities and velocities (almost 0), thus resulting in a worsened fertilization and hatching rate. Sperm was sampled with a syringe containing an immobilizing solution (IS), allowing an IS:sperm ratio of 2:1, under aerobic conditions at 0–4°C. This sperm solution was stored for 10 h and untreated sperm was collected prior to fertilization as a control. Spermatozoa quality was evaluated for the cell motility and velocity parameters and also for fertilization ability and hatching rate. Results obtained for tench sperm motility, velocity, fertilization and hatching rate showed that only sperm collected in the various immobilizing solutions can be successfully used for artificial insemination and preservation after 10 h at 0–4°C. The best immobilizing solution was found to be KUROKURA 180 (180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2· 2H2O and 2.38 mM NaHCO3), giving a fertility and hatching rate of 41%, with no change in rates after 10 h storage of sperm. Control sperm without immobilizing solution showed a fertilization and hatching rate of only 6–7%. 相似文献
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Effect of different methods for the induction of spermiation on semen quality in European eel 总被引：2，自引：0，他引：2
Juan F Asturiano Luz Pérez Daniel L Garzón David S Peñaranda Francisco Marco-Jiménez Silvia Martínez-Llorens Ana Tomás & Miguel Jover 《Aquaculture Research》2005,36(15):1480-1487
Five hormonal treatments with human chorionic gonadotropin (hCG) were tested for the induction of maturation and spermiation in male farmed eels. The main aim was to optimize previously used hormonal treatments to achieve shorter induction treatments, longer spermiation periods and/or higher sperm quality. Fish treated for just 3 weeks (treatment E) or until the onset of spermiation (treatment C) showed the worst results, while the treatment consisting of weekly administration of 1.5 IU hCG g?1 fish (treatment A) induced the highest percentage of spermiating males, the highest number of sperm samples and sperm volumes and densities similar to the rest of the treatments (B: half hormone dosage, or D: biweekly administration). Evaluation of the sperm quality was performed by computer‐assisted sperm analysis (CASA), considering the percentage of total motile spermatozoa, the percentage of fast and medium‐velocity spermatozoa, as well as different motility parameters. Sperm samples from A‐D groups showed between 44% and 54% motile spermatozoa, and between 10% and 15% fast spermatozoa, while samples from E‐treated males showed 0% motile cells. No significant differences were found in the spermatozoa straight line velocity (VSL), curvilinear velocity (VCL) or the angular velocity (VAP), neither spermatozoa beating cross frequency (BCF) between A–D groups. 相似文献
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Sperm of gilthead seabream, Sparus aurata, was diluted with solutions of different osmolarities and pH. The effect of the different diluents on sperm motility (intensity and percentage of motile sperm) was studied. Motility was induced as early as 10 s after mixing the sperm with diluents having an osmotic pressure higher than 500 mOsm/l. The intensity of motility decreased when the osmotic pressure was reduced, and was zero or significantly inhibited when the osmotic pressure of the diluent (300–380 mOsm/l) was close to that of the fish's seminal plasma (364.6±3.03 mOsm/l). The pH of the diluent did not have any effect on sperm motility (in a range of 6.8 to 8.9). A diluent which prevents spermatozoa motility (osmotic pressure 375 mOsm/l and pH 7.35) was successfully used to cryopreserve S. aurata sperm at −196°C. This diluent is considered promising for the long-term preservation of gilthead seabream sperm. 相似文献
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观察了褐牙鲆(Paralichthys olivaceus)精子在室温和低温下的活力与寿命,并应用计算机辅助精子分析系统(CASA)对超低温冷冻前后褐牙鲆精子的运动特征进行了分析,结果表明:褐牙鲆精子在室温(25℃)下,可存活4 d,在低温(4℃)下可存活7 d;鲜精的活力为(87. 74±5. 47)%,解冻后,精子的最高活性为(84. 00±3. 67)%;激活0. 5 min时,冻精与鲜精的运动精子占总精子数的百分率(MOT)无显著性差异(P>0. 05),但精子平均曲线运动速度(VCL)、平均直线运动速度(VSL)、平均路径运动速度(VAP)和精子运动路线的曲折程度(LIN)都有显著性差异(P <0. 05);激活4min和10min时,冻精与鲜精的MOT、VCL、VSL、VAP和LIN间都有显著性差异(P <0. 05)。鲜精激活0. 5 min后,直线运动、曲线运动、左右摆动和不运动的精子数目占总精子数的百分比分别为(24. 49±3. 87)%、(48. 53±4. 55)%、(24. 72±2. 86)%和(2. 27±1. 22)%;冻精激活0. 5min后,直线运动、曲线运动、左右摆动和不运动的精子数目占总精子数的百分比分别为(18. 58±1. 33)%、(35. 67±3. 00)%、(35. 24±2. 67)%和(10. 51±1. 33)%。随着激活时间的延长,褐牙鲆鲜精和冻精的运动状态均发生了改变,直线运动和曲线运动的精子数目逐渐减少,而不运动和左右摆动的精子数目逐渐增加。 相似文献
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半滑舌鳎精子冷冻保存对于人工繁殖育苗、杂交育种、雌核发育及其性别控制研究具有重要的意义,为此,本文对半滑舌鳎精子冷冻保存方法进行了研究。分别利用2．8mol／L的二甲基亚砜（DMSO）、甘油（Gly）和1,2-丙二醇（PG）冷冻保存该鱼精子。结果显示,DMSO冷冻保存精子的活力较高。利用MPRS＋2．8mol／L DMSO以1：0．5、1：1、1：1．5和1：2的比例稀释并冷冻精子,1：1比例在冻前能够抑制精子的运动,冻后活力可达82．50±3．54％,显著高于其他稀释比例（P〈0．05）。分别利用冷冻保存液A（MPRS＋2．8mol／L DMSO）和B（TS-2＋2．8mol／LDMSO）稀释平衡精子,精子在A中的冻前快速运动时间、寿命分别为37．75±6．45S和145．00±78．98S,与鲜精无显著差异（P〉0．05）。利用以上两种冷冻稀释液冷冻保存精子,精子在A液中的冻后活力和寿命分别可达53．50±6．69％和98．00±13．51s,冷冻效果优于B液（P〈0．05）。冷冻后精子的受精率和孵化率分别为55．00±5．00％和35．00±13．23％,受精率与鲜精无显著性差异（P〉0．05）,因此认为MPRS＋2．8mol／L DMSO可用于半滑舌鳎精子的冷冻保存。 相似文献
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Experiments on short-term preservation of sperm were performed with Atlantic salmon (Salmo salar). Fertility was maintained for up to 10 days when 2 mm thick samples were stored at 0° C under an oxygen atmosphere in the presence of antibiotics (125 IU penicillin and 125 μg streptomycin per ml sperm). Fertility was completely lost after 24 days. Sperm stored without antibiotics fertilized 100% of eggs after 6 days.
Cryopreservation was carried out with milt from Atlantic salmon and sea trout (Salmo trutta). Semen mixed with extender was frozen on dry ice (pellets) with subsequent storage in liquid nitrogen. Sperm pellets were thawed in a 0.12-M NaHCO3-solution (10° C) before insemination. The suitability of an extender as previously described by Stoss and Holtz and of a 0.3-M glucose solution with the addition of 10% DMSO, was tested on two different batches of sperm and eggs in Atlantic salmon and sea trout. In addition, the extender earlier reported by Mounib and an aqueous solution of 10% DMSO were only used in Atlantic salmon with one batch of gametes.
Insemination with cryopreserved Atlantic salmon sperm resulted in 36 to 91.3% eyed eggs (control = 100). The differences were caused by the type of extender and the batch of gametes employed. The very simple extender consisting of 0.3 M glucose and 10% DMSO only was the most successful. Results with cryopreserved sea trout sperm ranged between 38.6–54.8% eyed eggs, showing no difference between treatments. 相似文献
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Milt from paddlefish ( Polyodon sparhula ) was collected, examined for motility, and pooled. Six different solutions (dechlorinated tap water, ovarian fluid, bacteriostatic water, distilled water, 0.9% sodium chloride saline water, and milt plasma) were examined for activation and duration of spermatozoal motility. Most solutions except saline solution and milt plasma promoted activation of spermatozoa. Dechlorinated tap water was found to be the best activator with significantly longer duration of spermatozoal motility than the other tested solutions. Saline solution and milt plasma appear to be useful as carrier solutions because of their property of maintaining inactive, viable spermatozoa.
Paddlefish milt in three treatments was evaluated for chilled storage [without additives (MF); with 500 IU penicillin and 500 mg streptomycin mix in milt plasma (MP); and 0.9% saline solution (MS)]. The three treatments were stored at 2.4 ± 0.9 C in a precooled, moisture-saturated oxygen environment and observed every 24 h for 9 d. Motility of activated spermatozoa in MS (7 d) was significantly longer than MF or MP treatments (7 d). Spermatozoa in MS had the longest chilled storage time (8 d). 相似文献
Paddlefish milt in three treatments was evaluated for chilled storage [without additives (MF); with 500 IU penicillin and 500 mg streptomycin mix in milt plasma (MP); and 0.9% saline solution (MS)]. The three treatments were stored at 2.4 ± 0.9 C in a precooled, moisture-saturated oxygen environment and observed every 24 h for 9 d. Motility of activated spermatozoa in MS (7 d) was significantly longer than MF or MP treatments (7 d). Spermatozoa in MS had the longest chilled storage time (8 d). 相似文献
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The effects of different carbon dioxide (CO2) levels on the short-term storage of semen samples from hatchery-produced steelhead (Oncorhynchus mykiss) were evaluated. Sperm motility and fertilizing ability were significantly reduced following 4 h incubation under a relatively modest (0.9 kPa = 1%) amount of CO2. The dose-dependent reductions, however, were not the result of cell death as sperm viability was unaltered even at the highest (5.2 kPa = 5.6%) CO2 exposures. Reductions in sperm motility and fertilizing ability were reversible. Although previous work has indicated a direct relationship between salmonid sperm motility and sperm ATP content, the inhibitory effects of CO2 on sperm motility were not the result of reduced sperm ATP levels. Decreasing the pH of the seminal fluid (to below 7.5) significantly reduced sperm motility. However, this effect was only observed after prolonged (4 h) exposure; short-term (1 min) exposure to this lowered pH did not alter sperm motility. Moreover, acetazolamide, a carbonic anhydrase inhibitor, attenuated the inhibitory effects of CO2 on sperm motility. These results suggest that CO2 inhibits steelhead sperm motility and therefore fertility in a dose-dependent manner, by reversibly lowering intracellular pH. 相似文献