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1.
2015年3-4月及2017年1月在南海外海进行了两次罩网渔船兼作金枪鱼延绳钓的捕捞试验,基于试验数据分析延绳钓钓钩深度及渔获水层分布,以期优化改进试验钓具,有效提高南海金枪鱼延绳钓捕捞效益。研究发现:1)经济渔获以剑鱼(Xiphias gladius)与黄鳍金枪鱼(Thunnus albacares)为主,剑鱼的单位捕捞努力量渔获量(CPUE,kg·千钩-1)为所有渔获中最高; 2)钓钩深度(D)与钩位(n)、风速(SW)、风流合压角(γ)成正相关,与漂移速度(SD)、投绳船速(SV)呈负相关,回归方程为:D=10. 259n-37. 247SD-29. 878SV+6. 940SW+23. 493γ+35. 633; 3)模型的预测结果与实测数据间无显著性差异,相对权重表明各影响因子中钩位具有最大的相对重要性; 4)试验中拟合钩深分布范围为35. 08~110. 80 m,剑鱼上钩率与CPUE最高的水层为60~80 m,黄鳍金枪鱼上钩率与CPUE最高的水层为80~100 m; 5) 60~80 m水层主要经济渔获CPUE最大且钓钩数目最多,认为钓钩水层分布较合理。  相似文献   

2.
磁珠富集法制备大口鲶的微卫星分子标记   总被引:3,自引:0,他引:3  
全迎春 《水产学报》2006,30(3):335-340
2004年3月16日-6月8日,在广东广远渔业集团有限公司玻璃钢大滚筒冷海水金枪鱼延绳钓渔船“华远渔19”号,对印度洋马尔代夫海域进行金枪鱼渔业调查。所获数据资料包括:①CTD测定的温度、盐度和深度数据,TDR测定的实际钩深;②作业参数,包括每次作业的钩数、投绳位置、投绳时间、航向、航速、出绳速度、两浮子间的钩数、起绳位置、起绳时间等;③渔场环境数据,包括风速、风向、钓具对地漂移速度和方向;④渔获统计,包括大眼金枪鱼的渔获尾数以及部分大眼金枪鱼的钓获钩号;⑤生物学数据,包括起捕时已死的大眼金枪鱼的体温。分析计算的方法和步骤为:①由理论公式推算出每次作业时各钩号的理论深度;②应用逐步回归的方法,建立理论钩深与实测的平均钩深之间的数学关系模型;③根据实际平均钩深模型计算出每个钓钩的平均作业深度;④根据CTD测得的作业地点的温度、盐度的垂直分布(41次),获得分布曲线。由此,以计算的钩深,从曲线上查出该尾鱼捕获水深处的温、盐数据;⑤根据121尾大眼金枪鱼的取样数据,推算出各水层、水温段、盐度段的渔获率;⑥根据起捕时已死的34尾大眼金枪鱼(鱼体发硬)的体温、以死鱼体温为引数,在曲线上查出该尾鱼捕获水深和盐度;最后,对钓获的大眼金枪鱼的钓获水层、水温和盐度进行了总体分析。分析结果表明:在马尔代夫海域,捕获大眼金枪鱼的水层为50~210m、水温范围为13.0~29.9℃、盐度范围为35.00~35.79;渔获率最高的水层为70~90m、水温范围为27.0~27.9oC、盐度范围为35.70~35.79;捕获时已死鱼的捕获水层、水温和盐度推算数据为63~203m、14.0~27.0℃和34.94~35.42,主要集中在63~134m、16.0~27.0℃和35.30~35.42。  相似文献   

3.
根据中国金枪鱼延绳钓渔业观察员于2018年9-12月在北太平洋公海(150°W-164°W,30°N-37°N)采集的金枪鱼延绳钓渔获数据,分析了钓钩的垂直分布及浸泡时间与渔获物之间的关系。结果表明,11种主要渔获物钓获深度差异很大,鲣(Katsuwonus pelamis)的平均钓获深度最浅,大青鲨(Prionace glauca)最深;除大青鲨和帆蜥鱼(Alepisaurus ferox)外,其他兼捕鱼类的钓钩深度分布与大眼金枪鱼(Thunnus obesus,目标鱼种)之间差异显著;随着浸泡时间的增加,总渔获率呈先增加后下降的趋势,10~14 h内渔获率较高,且在第10小时达到峰值[1.44尾·(千钩·h)-1];浸泡时间在10~16 h内大眼金枪鱼的渔获率较高,且在第11小时达到峰值[0.45尾·(千钩·h)-1]。研究结果可为渔业生产和CPUE的标椎化提供参考,为制定有效减少兼捕鱼种的管理措施提供科学依据。  相似文献   

4.
通过模型分析环境变量对延绳钓大眼金枪鱼渔获率的影响,评估适宜垂直活动空间对大西洋大眼金枪鱼延绳钓渔获率的作用。首先采用回归分析检验环境变量对延绳钓渔获率(由单位捕捞努力渔获量(catch per unit fishing effort,CPUE)表示)的影响显著性,结合时空变量,采用GAM(generalized additive model)模型分析各变量对大眼金枪鱼CPUE非线性作用。模型结果表明,环境因子和时空变量对热带大西洋延绳钓大眼金枪鱼渔获率空间分布影响明显。大西洋大眼金枪鱼延绳钓的高渔获率月份出现在夏季和冬季,空间上在赤道以北和30?~50?W。12℃等温线深度对大眼金枪鱼延绳钓渔获率的影响表现为抛物线形状,高渔获率出现在深度较浅的250 m水层,随着12℃等温线深度的增加,大眼金枪鱼延绳钓渔获率降低。温跃层下界深度和深度差对大眼金枪鱼延绳钓渔获率的影响都是穹顶状。随着温跃层下界深度值和深度差由小变大至200 m,延绳钓渔获率递增;温跃层下界深度和深度差超过200 m后,延绳钓渔获率变小。温跃层下界深度和深度差对大眼金枪鱼延绳钓渔获率影响显著的水层分别是200 m和50 m。研究结果显示,12℃等温线深度和温跃层对热带大西洋延绳钓大眼金枪鱼渔获率影响是交叉的,在大眼金枪鱼适宜垂直活动水层受限到和延绳钓作业深度相同时,延绳钓渔获率最高;在适宜垂直活动空间过深或者过浅时,延绳钓渔获率都变小,但可以通过改变作业方式提高渔获率。采用延绳钓CPUE进行渔场和资源评估要考虑金枪鱼适宜垂直活动空间。  相似文献   

5.
金枪鱼延绳钓环形钩和圆形钩钓获率比较   总被引:3,自引:0,他引:3  
根据"华远渔18号"和"华远渔19号"两艘冰鲜金枪鱼延绳钓渔船2005年9月15日~12月12日在印度洋热带公海水域(0°47′~10°16′N,61°40′~70°40′E)作业期间收集的数据,以每千钩钓获尾数和平均净重,对环型钩与圆型钩对大眼金枪鱼和黄鳍金枪鱼的选择性、钓获率差异等进行了比较研究。结果表明:不同钓具漂移速度下环型钩与圆型钩各自对大眼金枪鱼和黄鳍金枪鱼的选择性无显著差异;不同钓具漂移速度下环型钩与圆型钩之间对大眼金枪鱼和黄鳍金枪鱼的钓获率无显著差异;环型钩与圆型钩之间对大眼金枪鱼和黄鳍金枪鱼渔获物净重无显著差异。  相似文献   

6.
大西洋中部大眼金枪鱼垂直分布与温度、盐度的关系   总被引:11,自引:2,他引:11  
根据2001年7月4日至10月27日3艘在大西洋中部公海海域作业的中国金枪鱼延绳钓船上随机观测到的大眼金枪鱼(Thunnus obesus)的上钩钩号,应用悬链线钩深计算公式,分别计算出各钩号的钩深;根据STD仪测得的温度、盐度的垂直分布以钩深为引数,查出该尾鱼捕获时的温度和盐度数据。根据大眼金枪鱼的取样数据,利用频度统计的方法,推算出各水层、水温、盐度范围的渔获率。渔获率最大的水层、水温、盐度范围为大眼金枪鱼的最适水层、水温和盐度范围;渔获率为前3位的水层、水温、盐度范围为大眼金枪鱼活动较频繁的水层、水温和盐度范围。结果表明:大西洋中部,大眼金枪鱼的最适水层为240.00~269.99m水深、最适水温为12.00~12.99℃、最适盐度为35.00~35.09;大西洋中部渔场大眼金枪鱼活动较频繁的水层为240.00~329.99m水深、水温为10.00~12.99℃、盐度为35.00~35.29。  相似文献   

7.
灯光罩网渔船兼作金枪鱼延绳钓捕捞试验   总被引:2,自引:0,他引:2  
2015年3月~4月利用"粤电渔42212"灯光罩网渔船在南沙北部海域开展了金枪鱼延绳钓捕捞试验。延绳钓试验以不影响灯光罩网的正常作业为前提。试验期间,灯光罩网作业40晚,放网297次,平均渔获率336.4kg·h-1,总渔获质量129.6 t,鸢乌贼(Sthenoteuthis oualaniensis)占85.31%,黄鳍金枪鱼(Thunnus albacaes)占0.21%。延绳钓作业7次,放钩2 700枚,总渔获质量1 281.6 kg,剑鱼(Xiphias gladius)和黄鳍金枪鱼分别占72.88%和11.16%;延绳钓平均上钩率和CPUE分别为25.74尾·千钩-1和427.48 kg·千钩-1,其中黄鳍金枪鱼为1.90尾·千钩-1、52.74 kg·千钩-1,剑鱼为3.76尾·千钩-1、291.86 kg·千钩-1。试验证明,金枪鱼延绳钓和灯光罩网的渔场分布恰好一致,作业时间没有冲突。相比发展专业钓船,罩网渔船兼作延绳钓具有投资金额少、生产成本低的优势。建议通过探捕拓展和延长外海渔期,并研究解决金枪鱼保鲜问题。  相似文献   

8.
印度洋公海温跃层与黄鳍金枪鱼和大眼金枪鱼渔获率的关系   总被引:10,自引:0,他引:10  
宋利明  张禹  周应祺 《水产学报》2008,32(3):369-378
2005年9月15日-12月12日,金枪鱼延绳钓渔船"华远渔18、19号"对印度洋公海进行了金枪鱼渔业调查.利用多功能水质仪(XR-620)、温盐深仪(CTD SBE37SM)和微型温度深度计(TDR-2050)等获取海洋环境数据,得出作业海域的温跃层深度和强度,结合每天作业时记录的渔获数据,分别计算黄鳍金枪鱼和大眼金枪鱼在温跃层内和深水层的渔获率,研究温跃层与黄鳍金枪鱼和大眼金枪鱼渔获率之间的关系.结果表明:(1)2艘船分别有60.9%和60.0%的作业天数中,温跃层内黄鳍金枪鱼渔获率较高,"华远渔18号"船在温跃层内和温跃层以下的平均渔获率分别为18.22尾每千钩和6.04尾每千钩,"华远渔19号"船温跃层内和温跃层以下平均渔获率分别为2.22尾每千钩和1.31尾每千钩.通过t-检验成对双样本均值分析,温跃层以内和温跃层以下黄鳍金枪鱼总平均渔获率有显著性差异(P=0.02<0.05),温跃层以内比温跃层以下的渔获率明显要高;(2)2艘船分别有69.6%和100%的作业天数中,温跃层以下水深大眼金枪鱼渔获率较高,"华远渔18号"温跃层以内和温跃层以下平均渔获率分别为4.18尾每千钩和4.88尾每千钩."华远渔19号"温跃层以内和温跃层以下平均渔获率分别为0.10尾每千钩和2.57尾每千钩.大眼金枪鱼渔获率在温跃层以下水深较高.通过t-检验成对双样本均值分析,温跃层以内和温跃层以下大眼金枪鱼总平均渔获率无显著性差异(P=0.070.05),但对"华远渔19号"船的渔获率数据经t-检验发现大眼金枪鱼在温跃层以内和温跃层以下的渔获率有显著性差异(P=0.00<0.05).  相似文献   

9.
金枪鱼延绳钓渔获性能主要按目标鱼种和兼捕物种渔获效率进行评价。对其研究有助于改进延绳钓渔具渔法, 提高目标鱼种捕捞效率和减少兼捕。本文以时间顺序为主对国内外关于金枪鱼延绳钓渔获性能研究的文献进行梳理, 从钓具选择性、钓钩深度、饵料选择性、环境因素以及钓具浸泡时长等方面概括了金枪鱼延绳钓渔获性能的研究进展, 并提出存在的不足和建议, 为金枪鱼延绳钓渔获性能的研究提供参考。前人研究取得的成果有: (1)不同鱼种最佳作业深度和钓具浸泡时长不同; (2)较大尺寸的圆形钩能减少兼捕; (3)拟饵也具有选择性, 鱼类饵料和蓝色染色饵料有利于减少兼捕; (4)具体水层的环境因素对延绳钓渔获性能影响较大。建议今后金枪鱼延绳钓渔获性能研究应: (1)确定钓钩最佳沉降速度和深度; (2)分水层建立不同物种渔获性能预测模型; (3)针对不同的目标鱼种探索最佳尺寸和钩形; (4)研究不同气味和颜色的饵料或拟饵对物种选择性的影响; (5)考虑诱饵、钓钩类型和尺寸和钓具浸泡时长对渔获率、死亡率、兼捕率和兼捕物种释放后存活率的潜在协同效应。  相似文献   

10.
海龟是金枪鱼延绳钓误捕的主要物种之一,如何减少其误捕是国际社会关切的热点。海龟栖息水层和环境不同于金枪鱼,钓钩和饵料等也影响海龟的误捕。文章从钓钩深度、钓钩的选择性、饵料的选择性、环境因子以及声学因素等方面综述了国内外金枪鱼延绳钓渔业中缓解海龟误捕方法的研究进展。控制延绳钓钓钩深度是缓解海龟误捕的有效途径;较大尺寸的圆型钩可以有效缓解海龟误捕;蓝色染色饵料或许能缓解海龟误捕;避开海龟偏好的海表面温度进行作业对缓解海龟误捕有效;应用声学干扰可缓解海龟误捕。建议金枪鱼延绳钓渔业中缓解海龟误捕研究应做到:确定钓钩最佳沉降深度;探索减少海龟误捕和保证金枪鱼渔获率的钓钩最佳尺寸和钩型;研究染色饵料或拟饵等对海龟选择性的影响;考虑诱饵、钓钩类型、钓钩尺寸和钓具浸泡时长对海龟误捕和海龟释放后存活率的潜在协同效应;继续开展利用声学区分海龟和金枪鱼及缓解海龟误捕方法的研究。  相似文献   

11.
12.
The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.  相似文献   

13.
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.  相似文献   

14.
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus.  相似文献   

15.
In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.  相似文献   

16.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.  相似文献   

17.
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality).  相似文献   

18.
19.
A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg.  相似文献   

20.
Cholecalciferol (Vitamin D3) increased plasma inorganic phosphate concentration in American eels,Anguilla rostrata, in a dose-dependent fashion. This response was more marked in phosphate loaded fish. In control as well as phosphate loaded eels the hyperphosphatemic response to D3 was associated with a sharp reduction in renal phosphate clearance relative to14C-polyethelene glycol (PEG) clearance. Glomerular filtration and urine flow rates were not affected by D3. As renal phosphate clearance, even in phosphate loaded eels, never significantly exceeded that of PEG, it is suggested that D3 reduced the relative clearance rate of phosphate by increasing renal phosphate reabsorption rather than by reducing the tubular secretion of phosphate.  相似文献   

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