共查询到20条相似文献，搜索用时 156 毫秒
1.
<正>自1957年lsaacs和lindenmann发现干扰素以来,干扰素已经显示出极强的抗病毒、抗肿瘤以及免疫调节活性和应用前景。因而,干扰素研究越来越受到人们的广泛关注。目前,动物干扰素主要停留在基础研究和临床研究试验阶段,且大多数集中于鸡、猪、鱼等少数动物,但近年来也取得不小的进步,目前已有商品化的鸡、猪、犬等重组干扰素产品面市。本品就近年来国内动物干扰素的应用和在家禽等方面的应用作以介绍。 相似文献
2.
3.
4.
5.
6.
干扰素是一类仅由脊椎动物单核细胞和淋巴细胞在受到病毒或其他诱生剂的作用时产生的一种具有抗病毒、抗肿瘤、免疫调节等多种生物学活性的分泌性可溶糖蛋白。随着基因工程方法的发展,外源表达干扰素已经进入了工业化生产阶段,并且大量投放市场,在医药、生物和农业等多个行业领域中广泛应用。近年来,随着人们对禽类干扰素研究的逐步深入,干扰素在一些禽类病毒性疾病及肿瘤性疾病治疗方面取得的良好进展。本文现就家禽干扰素的分类,家禽干扰素的抗病毒活性以及家禽基因工程重组干扰素的应用做以综述。 相似文献
7.
8.
9.
10.
干扰素是由脊椎动物细胞产生的分泌型糖蛋白,具有广谱抗病毒和增强免疫应答的作用,在免疫应答调控中处于中心地位。干扰素在生物体中普遍存在,现已经证明在人及小鼠、羊、兔、犬、鼬等哺乳动物,以及大量野生动物、鱼类、龟类和昆虫等都有干扰素类似物质存在。干扰素在医学和兽医学临床方面疗效显著,为病毒性和肿瘤性疾病的治疗带来了新的希望。科研工作者除研究其基因结构、作用机理外,也在不断探索基因工程干扰素的临床应用,改善和提高临床治疗效果。 相似文献
11.
干扰素是一种诱生性糖蛋白,有抗病毒、抗肿瘤与免疫调节等多种生物学作用.当畜禽感染病毒时,干扰素是最先发挥作用的抵抗分子.干扰素参与早期黏膜免疫并通过产生一系列与免疫调节相关的细胞因子来活化机体的天然免疫与适应性免疫应答从而使机体处于抗病毒状态.干扰素因其重要的生物学功能,在流行性病毒病防治的应用中越来越广泛.本文从不同... 相似文献
12.
The effect of hydrocortisone on bovine interferon production in vitro was studied. Infectious bovine rhinotracheitis virus was used as an inducer. Interferon was assayed by the plaque-reduction method in bovine fetal kidney cultures, using vesicular stomatitis virus as challenge virus. Hydrocortisone decreased interferon production in bovine fetal spleen and peripheral blood leukocyte cultures. Hydrocortisone did not decrease interferon production by bovine alveolar macrophages, in 1 experiment. Properties of viral inhibitors were those of interferon. 相似文献
13.
自从1986年我国首次自主成功克隆猪,克隆技术在猪的研究获得较大推进,国内陆续报道了地方品种猪的成功克隆。然而,对于克隆猪在生产中的应用研究,暂时未见有较多报道。本文从动物克隆技术的发展、国内外研究进展以及克隆技术在生猪生产应用中存在的难点和缺点进行阐述,进一步推动猪克隆技术在猪生产中应用。 相似文献
14.
猪用增仔促孕素是用猪脾脏细胞生产的一种干扰素,它能促使母猪在妊娠识别期处于良好状态,能提高母猪子宫对胚胎附植的承载能力,改善母猪的繁殖性能,提高母猪的窝平产仔数。本文就猪用增仔促孕素即猪脾细胞干扰素的研制,生产和生产工艺进行了研究,并探索出整套猪脾细胞干扰素的生产工艺。猪脾细胞干扰素其原料来源广,成本低,具有较高的推广价值。 相似文献
15.
M Kandefer-Szerszen J Filar A Szuster-Ciesielska W Rzeski 《DTW. Deutsche tier?rztliche Wochenschrift》1992,99(11):440-443
The influence of spontaneous ketosis on interferon alpha and gamma production in blood leucocytes and on PHA induced lymphocyte blastogenic response was investigated. Twenty three cows 4.13 +/- 2.8 weeks after calving were divided into three experimental groups on the basis of blood ketone bodies, glucose and free fatty acids concentrations. The leukocytes of cows with clinical symptoms and the highest concentration of ketones and free fatty acids in blood responded with the lowest levels of interferons alpha and gamma to three interferon inducers: Newcastle Disease Virus (NDV), phytohemagglutinin (PHA) and concanavalin A (ConA). Depression in interferon PHA stimulated synthesis correlated with a very low mitogenic response of blood lymphocytes. Blood leukocytes of cows with subclinical ketosis, characterized by mild clinical symptoms and a lower concentration of ketones in blood in comparison to cows with clinical ketosis, responded better to interferon and mitogenic stimulation; however, the interferon titer and blastogenesis were still lower than in leukocytes of healthy cows. Correlation between the stage of ketosis and the level of interferon production in milk leukocytes was also observed. A possible relationship between the suppression of interferon production in blood leukocytes and the increased concentration of ketone bodies in blood is discussed. 相似文献
16.
17.
18.
The production of interferon by porcine kidney (PK15) cell culture in response to viral and synthetic inducers was studied. The inducers used included a synthetic double-stranded polyribonucleotide, polyriboinosinic-polyribocytidylic acid (Poly I:C), swine influenza virus and three strains of pseudorabies virus. Following exposure to these inducers cell culture fluids were examined for interferon by the plaque-reduction method.
The Poly I:C and the swine influenza virus induced production of interferon by PK15 cell cultures, whereas, all three strains of pseudorabies virus at the two concentrations tested failed to induce production of interferon in vitro.
The antiviral substance produced in PK15 cells was identified as an interferon because it was pH stable, non-dialyzable, sensitive to trypsin, non-sedimentable, relatively heat stable, host-species specific and it possessed broad-spectrum antiviral activity. The latter was demonstrated by inhibition of vesicular stomatitis, vaccinia and pseudorabies viruses. Differences in interferon activity against the different viruses were observed.
相似文献19.
T Metianu B Bizzini 《Comparative immunology, microbiology and infectious diseases》1981,4(3-4):247-253
The i.v. or i.p. injection of delipidated whole cells of an aerobic Corynebacterium, designated as C. catarrahalis, resulted in the production of a low titre of interferon in mice. Two fractions, a particulate fraction, designated as CBA p40, and a soluble fraction, designated as CBA LS, could be isolated from the delipidated whole cells and were found to lead to the formation of more or less high interferon levels according to the route of administration. Furthermore, the CBA p40 fraction, when injected i.p., elicited the production of a maximum titre of interferon at the 24th hour (viral-like interferon). 相似文献
20.
在经济全球化和贸易一体化的前提下，全球各行各业都在以大数据为基础进行创新提高生产力，并将人工智能技术应用于人类生活生产的方方面面。国内许多生猪养殖企业也在生猪生产各环节中，不断采用智能化技术来提高养猪管理和生产效率。文章介绍了猪场的智能饲喂概念及生产工艺，并对应用效果和前景进行了阐述和分析。 相似文献