共查询到19条相似文献，搜索用时 378 毫秒
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《中国兽医学报》2017,(7):1268-1273
为了解广东地区猪戊型肝炎的感染情况,本试验于2015年在广东不同猪场收集了2月龄内猪胆汁73份,RTnPCR检测、全基因组测序、同源性及进化分析结果显示:猪感染戊型肝炎的阳性率为6.8%(5/73),5个ORF2部分核苷酸序列同源性为87.2%~99.4%,属于基因4型,4b、4h亚型。对4b亚型中的1个阳性样品(GZHD)株进行全基因测序,其核苷酸序列与swGX40(EU676172)同源性最高为96.5%,与广东株SS19(JX855794)同源性为93.9%,与人源戊型肝炎病毒(HEV)株CVS-Sie10(LC042232)同源性为94.8%。结果表明:广东地区猪群间HEV流行毒株仍以基因4型为主,且为揭示戊型肝炎的跨种传播提供了新的分子生物学依据。 相似文献
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《畜牧与兽医》2021,(6)
为了解四川地区猪源戊型肝炎病毒(hepatitis E virus, HEV)的感染情况,采集四川13个地区屠宰场的358份生猪组织样品,采用荧光RT-PCR进行HEV检测,对阳性样品进行开放阅读框(open reading frame, ORF)两部分序列测序、同源性及系统进化分析。结果显示:猪感染HEV的阳性率为4.75%,17个毒株ORF2部分序列之间同源率为85.4%～99.4%,均属于基因4型,其中4a亚型8株、4b亚型3株、4d亚型6株。与人、猪等不同宿主的HEV参考毒株比较,四川猪源HEV与我国主要流行的人源HEV遗传关系密切,其中10株与人源毒株同源率超过95%。结果表明:四川地区生猪HEV流行毒株以基因4型为主,具有较大的人畜共患风险,应有针对性、系统性地对其进行预防和控制。 相似文献
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《畜牧与兽医》2015,(10):10-13
为了解云南省猪群戊型肝炎感染情况,从云南省8个猪场采集166份新鲜猪粪便样品,用套式RT-PCR技术对戊型肝炎病毒(HEV)核酸进行检测,结果 21份样品阳性,阳性率为12.7%,感染率较高。通过PCR产物片段的测序分析,去除重复序列后得到11株基因序列。同源性分析结果表明,这11株毒株序列与选定的Gen Bank中基因1型、2型、3型和4型参考序列的核苷酸序列同源性分别为85.6%~87.8%,85.6%~87.8%,85.6%~95.6%和92.8%~98.9%,说明这些株毒株属于基因4型,与中国其他地区分离的毒株同源性最高,证实HEV的流行具有地理分布的特征。 相似文献
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猪是人兽共患病病原戊型肝炎病毒（HEV）Ⅲ、IV基因型的重要贮存宿主。为了解云南省临沧市猪HEV的遗传进化情况，从规模猪场、猪散养户以及猪屠宰场，随机采集粪便153份、肝脏36份和胆汁54份，采用巢氏PCR进行HEV ORF2基因片段扩增、克隆、测序和生物信息学分析。结果显示：在243份样品中，检出137 bp目标片段阳性61份，阳性率为25.1%(61/243)；对检出的阳性样品进行ORF2部分序列扩增，得到6份348 bp目标片段。6份348 bp目标片段间的核苷酸同源性为86.0%～95.6%，均为基因IV型；与HEVI—VⅢ型代表株的同源性分别为75.0%～81.7%、72.7%～77.0%、72.7%～79.3%、80.5%～94.1%、75.7%～80.7%、75.0%～81.0%、73.3%～80.3%和71.3%～76.1%，与我国人源代表毒株AJ272108BeijingHuman的同源性为81.3%～87.7%；基因亚型分析显示，6份348 bp目标片段中，除1份是4b亚型外，其他5份都属于4a亚型。结果表明，临沧市猪群中存在人兽共患病病原HEV基因Ⅳ型流行，且有通... 相似文献
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江西部分地区猪粪便戊型肝炎病毒核酸检测及序列分析 总被引：1，自引：0，他引：1
采集江西省A猪场2～3月龄猪粪便40份,B猪场2～3月龄猪粪便40份,利用反转录套式聚合酶链方法(RT-nPCR),检测戊型肝炎病毒(hepatitis E virus,HEV)RNA,结果表明,A猪场7份样品为阳性,阳性率17.5%.B猪场10份样品为阳性,阳性率25.0%.总阳性率21.3%.对4份PCR扩增阳性产物进行纯化并测序,利用生物学软件进行序列分析和进化树绘制,4个分离株ORF2 348 bp同源性为92.1%～97.5%,为同一基因型,与HEV1、HEV2、HEV3同源性分别为70.7%～77.4%、72.5%~75.7%和71.6%～76.8%,与HEV4型的同源性79.2%～83.9%.4个分离株与HEV4型的代表株T1在同一分支上,属基因4型.与中国大陆6株猪源HEV比较,同源性为79.0%～87.3%,提示中国大陆猪源HEV的基因型比较一致,同属HEV 4型. 相似文献
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通过已建立的动物戊型肝炎病毒（Hepatitis E virus,HEV）RT-nPCR方法,检测上海地区和新疆地区猪群样品。扩增HEV开放读码框架2（open reading frame 2,ORF2）保守区,产物为507 bp。经克隆、测序,获得20株上海株、3株新疆株序列,采用分子生物学软件与47株已知HEV进行同源性、进化关系分析。结果23株序列从同源性比对和进化关系分析均属HEVⅣ型,上海株间507 bp的核苷酸同源性为94.1%~100.0%,与上海猪源（DQ450072）、日本人源Japan1（AB369690）亲缘关系近;3株新疆株507 bp间的核苷酸同源性为86.1%~98.2%,与新疆猪源China SW1（AY594199）、中国人源T1株（AJ272108）亲缘关系近;上海株与新疆株间的核苷酸同源性为82.6%~100.0%,提示上海株与新疆株间存在差异。23株HEV在时间和地域关系分析,提示不同年份的新疆株存在差异,同年份不同猪场的新疆株进化关系存在差异,不同年份、不同区县的上海株进化关系也存在差异。 相似文献
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为了解东北边境地区野猪及放养杂交野猪群体猪戊型肝炎病毒(HEV)感染情况,于2015—2018年在吉林省、黑龙江省的中朝、中俄边境和内蒙古自治区境内加格达奇周边地区采集6月龄以上杂交野猪血清、粪便或肛拭子样品共520份,采集野猪血清和粪便样品共248份。ELISA检测、RT-nPCR检测、全基因组测序、同源性及进化分析结果显示,杂交野猪和野猪感染HEV的血清抗体总阳性率为34.1%(136/399);核酸总阳性率为1.56%(12/771),12份核酸阳性样品均来自杂交野猪,病毒基因组ORF2部分核苷酸序列同源性为85.4%～100.0%,属于基因4型,4a、4b亚型。对4a亚型的1份阳性样品(LJG-18)进行病毒全基因组扩增测序,其核苷酸序列与日本的人源毒株JKO-ChiSai98C同源性最高,为94.9%,与吉林省猪源毒株Ch-S-1同源性为90.2%。结果表明:东北边境地区放养杂交野猪群具有较高的HEV血清抗体阳性率,HEV流行毒株以4a亚型为主。本试验针对我国野猪及放养杂交野猪群体开展猪戊型肝炎流行病学调查,为该病的流行情况提供了新的科学数据,对我国养猪业健康发展和公共卫生安全具有重要意义。 相似文献
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为了解广西猪群中戊型肝炎病毒(HEV)基因型,利用套式RT-PCR用对南宁周边猪场采集到的104份新鲜猪粪便进行HEV检测、分离并成功扩增和克隆了11株阳性毒株的ORF2基因部分保守片段.序列测定结果表明,11株HEV ORF2序列自身核苷酸同源性为97.7%~100%,推导编码的氨基酸同源性为97.9%~100%;与4型HEV代表毒株Ch-S-1、Ch-T11、swGX40等的核苷酸同源性为84.6%~90.6%,而与其他各型之间的同源性较低.系统发育进化树结果表明,11株HEV广西株为基因4型,其中,10株HEV为4a亚型,1株毒株为4e亚型.结果表明,广西猪群中流行的HEV均为基因4型,且以4a亚型为优势流行毒株. 相似文献
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为调查规模化猪场中戊型肝炎病毒(HEV)感染和流行特征,首先在4个猪场采集血样80份,用酶联免疫吸附试验(ELISA)榆测抗-HEV IgG抗体.在此基础上,作者于2007-2009年采集规模化猪场肛拭子样品415份,及规模化猪场送检的临床病猪肝脏样品135份,应用巢式PCR(RT-nPCR)方法进行HEV RNA检测,将PCR扩增产物测序,利用Neighbour-joining法进行进化分析.结果:4个猪场80份血清中HEV阳性血清56份,抗-HEV抗体阳件率高达60.0%以上.8个规模化猪场中有5个猪场的肛拭子检测到HEV,肛拭子阳性率8.43%(35/415);从临床送榆的发病猪采集的肝脏样品中HEV阳性率为15.55%(21/135);HEV RNA最早检出日龄为7 d,最晚检出日龄为84 d.遗传进化分析表明:测序的38株阳性毒株之间同源性为92%～100%,均属于HEV基因4型.其中,从肛拭子中分离的毒株(FJ445406)与猪源毒株DQ279091株处在同一分支上;而肝脏中分离的毒株(GQ202266.1)和人源毒株处在一个亚支,属于4型巾的同一亚型,表明该毒株与人源毒株亲缘关系较近.本研究表明华中地Ⅸ猪群中普遍存在HEV感染,阳性率较高.本研究为规模化猪场HEV的防控提供了依据. 相似文献
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为进行国内猪戊型肝炎病毒(HEV)衣壳蛋白基因特征研究,参照GenBank中已发表的戊型肝炎病毒核苷酸序列,设计了1对扩增HEV衣壳蛋白(Y)基因的引物,利用RT-PCR等方法成功克隆出HEV甘肃分离株swCH189的Y基因cDNA片段,并与国内其他9株典型HEV分离株进行序列分析,用DNAstar软件进行序列同源性分析,PHYLIP软件绘制基因进化树。结果表明,swCH189株的Y基因与国内9株典型HEV分离株间的核苷酸序列同源性为79.6%～91.8%,推导的氨基酸序列同源性为91.4%～98.85%。基因进化树显示swCH189与基因Ⅳ型swCH25株亲缘关系最近,在同一分支上,属于基因Ⅳ型。 相似文献
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Identification of genotype 3 hepatitis E virus in fecal samples from a pig farm located in a Shanghai suburb 总被引：9，自引：0，他引：9
Strains of hepatitis E virus (HEV) genotypes 1 and 4 have been detected on the Chinese mainland although there have been no previous reports of zoonotic genotype 3 HEV. In the present study, 65 swine fecal specimens were collected from five pig farms located in different Shanghai suburbs. RT-PCR and nested PCR were undertaken using partial nucleotide sequences of Open Reading Frame 2 (ORF2) of HEV to detect HEV RNA. Genetic analysis was based on alignments of an amplified 150-nt ORF2 sequence. RT-PCR revealed 15 HEV positive samples among 65-pig fecal specimens examined. Phylogenetic analysis of the amplified sequences indicated seven HEV strains belonged to genotype 3 and eight strains to genotype 4. This is the first time that genotype 3 hepatitis E virus has been identified on the Chinese mainland. 相似文献
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Federico Marziali Julian Acosta Elisa Bolatti Santiago Mirazo Patricia Skejich Patricia Silva Julie Brassard Alejandro Costaguta Daniela Gardiol Ana Laura Cavatorta 《Zoonoses and public health》2019,66(7):729-738
Hepatitis E virus (HEV) is currently considered as a global health concern due to the recognition of its zoonotic transmission to humans, mainly from swine, and its association with the development of severe cases of hepatitis in human risk populations. The lack of updated data on HEV state of infection in swineherds of Argentina, and the necessity of robust technologies for its detection in complex biological samples, positions HEV as an emerging issue in public health. Here, we have optimized a RT‐qPCR with internal control for a more precise and accurate HEV RNA detection in swine stool samples. We implemented this optimized molecular tool to analyse the current epidemiological scenario of HEV infection in swine from the core region of commercial activity of Argentina. A total of 135 stool samples were collected from 16 different farms and tested for HEV presence, resulting in 11 positive cases (8.1%). Phylogenetic analysis demonstrated that all of them correspond to HEV genotype 3 and that different subtypes circulate in the region. Moreover, two of the detected strains presented a high nucleotide similarity with a previously identified isolate from human sewage discharges, suggesting the zoonotic transmission of HEV to humans. Collectively, this work provides a better understanding of HEV epidemiology in Argentina while contributes to the improvement of HEV detection technologies. 相似文献
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Hepatitis E virus (HEV) genotype 3 was first identified in swine raised on a Shanghai suburban pig farm in late 2006. To accurately determine the prevalence of HEV infections among Shanghai pig farms, 426 pig fecal samples were collected from 37 pig farms located in all 10 Shanghai suburban districts and tested for the presence of HEV RNA using RT-PCR. Genetic analysis based on an amplified 150-bp ORF2 fragment revealed 111 samples to be HEV positive, and the prevalence of HEV infection within the different districts varied between 0 and 41.7%. Thirty-two samples were sequenced, and phylogenetic analysis indicated that 10 isolates belonged to HEV genotype 4 and were most closely related to 3 human and 2 swine HEV strains, all of which had originally been isolated from Asian countries including Japan and China. The remaining 22 isolates belonged to genotype 3 and were most closely related to a strain of swine HEV, US-SW, isolated from pigs in the United States. Our data indicated that genotype 3 HEV was widespread among suburban Shanghai pig farms although further study is required to determine the source and zoonotic nature of the virus. 相似文献
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