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81.
Mohamed M’BAYE Guohua HUA Hamid Ali KHAN Liguo YANG 《The Journal of reproduction and development》2015,61(5):391-397
Inhibins are members of the TGFβ superfamily and act as suppressors
of follicle stimulating hormone (FSH) secretion from pituitary glands
via a negative feedback mechanism to regulate folliculogenesis. In
this study, the INHBB gene was knocked down by three
RNAi-Ready pSIREN-RetroQ-ZsGreen vector- mediated recombinant plasmids
to explore the effects of INHBB silencing on
granulosa cell (GC) cell cycle, apoptosis and steroid production
in vitro. Quantitative real-time polymerase chain
reaction, Western blot, flow cytometry and ELISA were performed to
evaluate the role of INHBB in the mouse GC cell
cycle, apoptosis and steroid production in vitro. The
results showed that the relative mRNA and protein expression of
INHBB in mouse GCs can be significantly reduced by
RNAi with pshRNA-B1, pshRNA-B2 and pshRNA-B3 plasmids, with pshRNA-B3
having the best knockdown efficiency. Downregulation of the expression
of INHBB significantly arrests cells in the G1 phase
of the cell cycle and increases the apoptosis rate in GCs. This was
further confirmed by downregulation of the protein expressions of
Cyclin D1, Cyclin E and Bcl2, while the protein expression of Bax was
upregulated. In addition, specific downregulation of
INHBB markedly decreased the concentration of
estradiol and progesterone, which was further validated by the
decrease in the mRNA levels of CYP19A1and
CYP11A1. These findings suggest that inhibin βB is
important in the regulation of apoptosis and cell cycle progression in
granulosa cells. Furthermore, the inhibin βB subunit has a role in the
regulation of steroid hormone biosynthesis. Evidence is accumulating
to support the concept that inhibin βB is physiologically essential
for early folliculogenesis in the mouse. 相似文献
82.
Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens 下载免费PDF全文
Zohreh Pourhossein Ali Ahmad Alaw Qotbi Alireza Seidavi Vito Laudadio Gerardo Centoducati Vincenzo Tufarelli 《Animal Science Journal》2015,86(1):105-110
This experiment was conducted to evaluate the effects of different levels of sweet orange (Citrus sinensis) peel extract (SOPE) on humoral immune system responses in broiler chickens. Three hundred 1‐day broilers (Ross‐308) were randomly allocated to treatments varying in supplemental SOPE added in the drinking water. The experimental groups consisted of three treatments fed for 42 days as follows: a control treatment without feed extract, a treatment containing 1000 ppm of SOPE and a treatment containing 1250 ppm of SOPE. All treatments were isocaloric and isonitrogenous. Broilers were vaccinated with Newcastle disease virus (NDV), avian influenza (AI), infectious bursal disease (IBD) and infectious bronchitis virus (IBV) vaccines. Antibody titer response to sheep red blood cells (SRBC) was higher in the group fed 1250 ppm of SOPE (P < 0.05) as well as for immunoglobulin G (IgG) and IgM. Similarly, antibody titer responses to all vaccines were constantly elevated (P < 0.05) by SOPE enrichment in a dose‐dependent manner. Relative weights of spleen and bursa of Fabricius were unaffected by treatments. Dietary SOPE supplementation may improve the immune response and diseases resistance, indicating that it can constitute a useful additive in broiler feeding. Thus, supplying SOPE in rations may help to improve relative immune response in broiler chickens. 相似文献
83.
Bose M Hao X Ju J Husain A Park S Lambert JD Yang CS 《Journal of agricultural and food chemistry》2007,55(19):7695-7700
The effect of a combination of (-)-epigallocatechin-3-gallate (EGCG) with fish oil on intestinal tumorigenesis in Apc (Min/+) mice fed a high-fat diet was investigated in the present study. The combined treatment of EGCG and fish oil for 9 weeks reduced the tumor number by 53% as compared to controls while neither agent alone had a significant effect. Apoptosis was significantly increased in all treatment groups. beta-Catenin nuclear positivity in adenomas from the combination group was lower than control mice, implicating the modulation of Wnt signaling by the combination. Fish oil and the combination significantly reduced prostaglandin E 2 (PGE 2) levels in small intestinal tumors as compared to controls, suggesting modulation of aberrant arachidonic acid metabolism by fish oil. Akt phosphorylation in adenomas was significantly reduced in all treatment groups, which may have contributed to the observed increase in apoptosis. The results indicate that a combination of low doses of EGCG and fish oil can inhibit tumor multiplicity in Apc (Min/+) mice. 相似文献
84.
Genetics and mechanism of resistance to deltamethrin in a field population of Spodoptera litura (Lepidoptera: Noctuidae) 总被引:2,自引:0,他引:2
BACKGROUND: Spodoptera litura (F.) causes enormous losses in many economically important crops. The genetics of insecticide resistance has been extensively studied in several insect pests, but there is a lack of information on S. litura. Therefore, the genetics and mechanisms of the resistance of S. litura to deltamethrin were investigated. RESULTS: Bioassays at generation G1 gave resistance ratios of 9, 5, 41, 52 and 49 for deltamethrin, cypermethrin, profenofos, chlorpyrifos and triazofos respectively, when compared with the susceptible Lab-PK strain. Bioassays at G4 with a deltamethrin-selected population (Delta-SEL) showed that selection gave resistance ratios of 63 and 7 for deltamethrin when compared with the Lab-PK and UNSEL strains respectively. Cross-resistance to other insecticides tested was observed in the selected population. A notable feature of the Delta-SEL strain was that resistance to deltamethrin, cypermethrin, profenofos and chlorpyrifos did not decline over the course of five generations. Synergism tests with microsomal oxidase (MO) and esterase-specific inhibitors indicated that the deltamethrin resistance was associated with MO and, possibly, esterase activity. Reciprocal crosses between the Delta-SEL and Lab-PK strains indicated that resistance was autosomal and incompletely dominant. A direct test of monogenic inheritance suggested that resistance to deltamethrin was controlled by more than one locus. CONCLUSION: Stability and dominance of resistance and cross-resistance suggest that insecticides with different modes of action should be recommended to reduce pyrethroid selection pressure. 相似文献
85.
Turan S Topcu A Karabulut I Vural H Hayaloglu AA 《Journal of agricultural and food chemistry》2007,55(26):10787-10794
The fatty acid, sn-2 fatty acid, triacyglycerol (TAG), tocopherol, and phytosterol compositions of kernel oils obtained from nine apricot varieties grown in the Malatya region of Turkey were determined ( P<0.05). The names of the apricot varieties were Alyanak (ALY), Cataloglu (CAT), C?loglu (COL), Hacihaliloglu (HAC), Hacikiz (HKI), Hasanbey (HSB), Kabaasi (KAB), Soganci (SOG), and Tokaloglu (TOK). The total oil contents of apricot kernels ranged from 40.23 to 53.19%. Oleic acid contributed 70.83% to the total fatty acids, followed by linoleic (21.96%), palmitic (4.92%), and stearic (1.21%) acids. The s n-2 position is mainly occupied with oleic acid (63.54%), linoleic acid (35.0%), and palmitic acid (0.96%). Eight TAG species were identified: LLL, OLL, PLL, OOL+POL, OOO+POO, and SOO (where P, palmitoyl; S, stearoyl; O, oleoyl; and L, linoleoyl), among which mainly OOO+POO contributed to 48.64% of the total, followed by OOL+POL at 32.63% and OLL at 14.33%. Four tocopherol and six phytosterol isomers were identified and quantified; among these, gamma-tocopherol (475.11 mg/kg of oil) and beta-sitosterol (273.67 mg/100 g of oil) were predominant. Principal component analysis (PCA) was applied to the data from lipid components of apricot kernel oil in order to explore the distribution of the apricot variety according to their kernel's lipid components. PCA separated some varieties including ALY, COL, KAB, CAT, SOG, and HSB in one group and varieties TOK, HAC, and HKI in another group based on their lipid components of apricot kernel oil. So, in the present study, PCA was found to be a powerful tool for classification of the samples. 相似文献
86.
Campera Marco Budiadi Budiadi Bušina Tomáš Fathoni Baladzuri Hafizh Dermody Janine Nijman Vincent Imron Muhammad Ali Nekaris K. A. I. 《Agroforestry Systems》2022,96(5-6):829-841
Agroforestry Systems - Complex agroforestry systems are suggested as a possible solution to reduce the effects of deforestation in the tropics while enhancing the livelihoods of local human... 相似文献
87.
Desneux Nicolas Han Peng Mansour Ramzi Arn Judit Brvault Thierry Campos Mateus R. Chailleux Anais Guedes Raul N. C. Karimi Javad Konan Kouassi Arthur J. Lavoir Anne-violette Luna Mara G. Perez-Hedo Meritxell Urbaneja Alberto Verheggen Franois J. Zappal Lucia Abbes Khaled Ali Abid Bayram Yunus Cantor Fernando Cuthbertson Andrew G. S. De Vis Raf Erler Fedai Firake Dnyaneshwar M. Haddi Khalid Hajjar M. Jamal Ismoilov Khasan Jaworski Coline C. Kenis Marc Liu Hao-tian Madadi Hossein Martin Thibaud Mazih Ahmed J. Messelink Gerben Mohamed Samira A. Nofemela Robert S. Oke Abiola Ramos Csar Ricupero Michele Roditakis Emmanouil Shashank Pathour R. Wan Fang-Hao Wang Ming-hui Wang Su Zhang Yi-Bo Biondi Antonio 《Journal of pest science》2022,95(1):17-39
Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato... 相似文献
88.
【目的】研究荨麻促进消化道平滑肌运动的植株主要部位,分析其可能的受体机制。【方法】用生物信号处理系统监测麻叶荨麻皮刺与内茎(不含皮刺)水提液对小鼠离体小肠平滑肌的影响。【结果】2倍浓度递增(0.1%~3.2%)的皮刺剂量依赖性增加小肠收缩幅度、收缩张力和收缩峰值(P<0.05),但对收缩频率无影响(P>0.05);而内茎浓度达0.4%后使小肠收缩频率减慢(P<0.05)。0.8%皮刺一次性用药后小肠收缩幅度、收缩张力、收缩峰值均显著升高(P<0.05),而同剂量内茎对小肠收缩无影响(P>0.05)。M-受体阻断剂ATr与0.8%皮刺联合用药可使收缩幅度显著减弱(P<0.05)。【结论】荨麻植株的皮刺对消化道平滑肌具有促进作用,且可能部分作用是通过激活M-受体而实现。 相似文献
89.
种植密度对玉米-大豆带状间作下大豆光合、产量及茎秆抗倒的影响 总被引:2,自引:0,他引:2
【目的】阐明玉米-大豆带状间作下大豆植株冠层在不同种植密度下的光环境变化规律,明确种植密度对间作大豆叶片光合特性、产量形成及茎秆抗倒的影响,为构建寡日照地区间作大豆合理群体密度提供理论参考。【方法】本研究以大豆(川豆-16)和玉米(正红-505)为试验材料。采用双因素随机区组设计,主因素为种植方式,设玉米-大豆带状间作和大豆带状单作2个水平,副因素为大豆的3个种植密度(PD1=17株/m2,PD2=20株/m2,PD3=25株/m2),研究种植密度对间作大豆冠层内部光环境变化、叶片光合特性、植株生长动态、田间倒伏率及产量构成等的影响。【结果】2年结果表明,在玉米-大豆带状间作系统中,大豆生长中后期受高位作物玉米遮荫和自荫性增加的影响,其植株群体冠层内部的光合有效辐射(PAR)、叶面积指数(LAI)、叶片光合能力、分枝数及产量显著降低,但受玉米影响的程度因大豆种植密度的不同而不同。在间作模式下,PD1和PD2处理的大豆植株群体冠层光合有效辐射比PD3处理分别增加了45.4%和24.8%,净光合速率分别增加了46.1%和12.3%,单株有效荚数分别增加了53.2%和27.2%,单株分枝数分别增加了270.4%和140.9%,田间倒伏率分别降低了50.3%和19.3%。相关性分析发现,间作大豆的田间倒伏率与冠层内部光合有效辐射、叶片净光合速率、茎秆抗折力、茎叶干物质比、单株分枝数及单株有效荚数呈显著负相关,与株高、叶面积指数和单株无效荚数呈显著正相关。【结论】在玉米-大豆带状间作模式下,20株/m2的大豆密度(PD2)有利于创造良好的群体冠层内部光环境,降低植株田间大豆倒伏率,增加光合产物积累,从而提高大豆产量。 相似文献
90.
Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds 下载免费PDF全文
Kashif NOOR Hafiza Masooma Naseer CHEEMA Asif Ali KHAN Rao Sohail Ahmad KHAN 《农业科学学报》2022,21(10):2818-2832
Transgenic cotton carrying the Cry1Ac gene has revolutionized insect pest control since its adoption, although the development of resistance in insect pests has reduced its efficacy. After 10 years of cultivating Bacillus thuringiensis (Bt) cotton with a single Cry1Ac gene, growers are on the verge of adopting Bt cotton that carries the double gene (Cry1Ac+Cry2A) due to its better effectiveness against insect pests. Thus, the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double gene. The expression levels of the Cry1Ac and Cry2A genes were evaluated in the leaves of 10 genotypes (2 parents and 8 F1 hybrids) at 30 days after sowing (DAS), while samples of leaves, bolls and flowers were taken from the upper and lower canopies at 70 and 110 DAS. The F1 hybrids were developed through reciprocal crosses between two Bt (CKC-1, CKC-2) and two non-Bt (MNH-786, FH-942) parents. The differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay (ELISA). The results showed that the MNH786×CKC-1 hybrid had the highest concentrations of Cry1Ac gene at 30 DAS (3.08 µg g–1) and 110 DAS (1.01 µg g–1) in leaves. In contrast, the CKC-2×MNH-786 hybrid showed the lowest concentrations of Cry1Ac gene at 30 DAS (2.30 µg g–1) and 110 DAS (0.86 µg g–1). The F1 hybrid FH-942×CKC-2 showed the highest concentrations of Cry2A gene at 30 DAS (8.39 µg g–1) and 110 DAS (7.74 µg g–1) in leaves, while the CKC-1×MNH-786 hybrid expressed the lowest concentrations of Cry2A gene at 30 DAS (7.10 µg g–1) and 110 DAS (8.31 µg g–1). A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS, whereas the lowest concentrations were observed at 110 DAS for both genes in leaves. When the expression pattern was compared between various plant parts in genotype CKC-2, it was found that leaves had higher concentrations of Cry1Ac (3.12 µg g–1) and Cry2A (8.31 µg g–1) at 70 DAS, followed by bolls (Cry1Ac (1.66 µg g–1) and Cry2A (8.15 µg g–1)) and flowers (Cry1Ac (1.07 µg g–1) and Cry2A (7.99 µg g–1)). The genotype CKC-2 had higher concentrations of Cry1Ac (3.12 µg g–1) and Cry2A (8.31 µg g–1) in the upper canopy but less accumulation (2.66 µg g–1 of Cry1Ac, 8.09 µg g–1 of Cry2A) in the lower canopy at 70 DAS. Similarly, at 110 DAS, the expression levels of Cry1Ac and Cry2A in upper and lower canopy leaves were 1.52 and 7.92 µg g–1, and 0.99 and 7.54 µg g–1, respectively. Hence, the current study demonstrates that different genotypes showed variable expression for both of the Cry1Ac and Cry2A genes during plant growth due to different genetic backgrounds. The Cry2A gene had three-fold higher expression than Cry1Ac with significant differences in expression in different plant parts. The findings of this study will be helpful for breeding insect-resistant double-gene genotypes with better gene expression levels of Cry1Ac and Cry2A for sustainable cotton production worldwide. 相似文献