Evaluation of Pax5 expression and comparison with BLA.36 and CD79αcy in feline non‐Hodgkin lymphoma          下载免费PDF全文

R. Felisberto  J. Matos  M. Alves  J. Cabeçadas  J. Henriques 《Veterinary and comparative oncology》2017,15(4):1257-1268

Paired box gene 5 (Pax5) is a widely used B‐cell marker for human and canine non‐Hodgkin's lymphoma (nHL); however, in the literature there is only one case report using Pax5 in a cat B‐cell lymphoma. The purposes of this study were to investigate the expression and detection of B‐cell specific activator protein (BSAP) using a monoclonal anti‐Pax5 antibody in feline nHL (FnHL) tissue samples to evaluate its diagnostic relevance as a B‐cell marker. A total of 45 FnHL samples in 45 cats were evaluated. B‐cell lymphoma was the most common immunophenotype (51.1%) for all the samples and T‐cell the most common immunophenotype (64.3%) for the gastrointestinal (GI) form. Pax5 stained 82.6% of all B‐cell lymphomas and no expression was found in any of the T‐cell lymphomas. Anti‐Pax5 antibody staining in FnHL is similar to that reported in human and canine counterparts and may offer an excellent B‐cell marker in cats.  相似文献   

Immortalization and characterization of a new canine mammary tumour cell line FR37‐CMT          下载免费PDF全文

L. R. Raposo  C. Roma‐Rodrigues  P. Faísca  M. Alves  J. Henriques  M. C. Carvalheiro  M. L. Corvo  P. V. Baptista  A. J. Pombeiro  A. R. Fernandes 《Veterinary and comparative oncology》2017,15(3):952-967

Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37‐CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD‐SCID mice. FR37‐CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E‐cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up‐regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR‐200c are also in accordance with the mesenchymal characteristics of FR37‐CMT cell line. FR37‐CMT shows a higher resistance to cisplatin (IC₅₀>50 µM) and to doxorubicin (IC₅₀>5.3 µM) compared with other CMT cell lines. These results support the use of FR37‐CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.  相似文献   

Characterization of Protein Hydrolysates from Fish Discards and By-Products from the North-West Spain Fishing Fleet as Potential Sources of Bioactive Peptides     

Andreia Henriques  Jos A. Vzquez  Jesus Valcarcel  Rogrio Mendes  Narcisa M. Bandarra  Carla Pires 《Marine drugs》2021,19(6)

Fish discards and by-products can be transformed into high value-added products such as fish protein hydrolysates (FPH) containing bioactive peptides. Protein hydrolysates were prepared from different parts (whole fish, skin and head) of several discarded species of the North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture and ash contents lower than 10% and 15%, respectively. The fat content of FPH varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The amino acids profiles of FPH are quite similar and the most abundant amino acids were glutamic and aspartic acids. All FPH exhibited antioxidant activity and those obtained from Atlantic horse mackerel heads presented the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and Cu²⁺ chelating activity. On the other hand, hydrolysates from gurnard heads showed the highest ABTS radical scavenging activity and Fe²⁺ chelating activity. In what concerns the α-amylase inhibitory activity, the IC₅₀ values recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and whole Atlantic horse mackerel, respectively. α-Glucosidase inhibitory activity of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme (ACE) inhibitory activity. Considering the biological activities, these FPH are potential natural additives for functional foods or nutraceuticals.  相似文献   

Effects of copper toxicity on growth and the uptake and translocation of metals in rice plants     

Fernando C. Lidon  Fernando S. Henriques 《Journal of plant nutrition》2013,36(8):1449-1464

Copper (Cu) contamination in the lower reaches of the Sado River in the Alcacer do Sal region of Portugal (a major rice producing area) has became a major pollution problem. In an attempt to study the changes in rice growth (Oryza sativa L.), the effect of excess Cu on the plasma membrane activity (as measured throughout proton extrusion) and membrane permeability as well as on the zinc (Zn), nitrogen (N), phosphorus (P), potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), boron (B), molybdenum (Mo), and aluminum (Al) net uptake and translocation was studied. Long‐term studies (30 days) were conducted with rice subjected to increasing Cu concentrations (ranging from 0.002 to 6.25 mg/L) showed a concentration increase of Cu in root and shoot tissues. Root proton extrusion increased 7.4‐fold between the 0.01 and the 1.25 mg/L Cu treatments, whereas its membrane permeability (as measured throughout the electrolytic conductance) revealed a marked increase after the 1.25 mg/L Cu treatment. Zinc concentrations decreased with increasing Cu levels in the nutrient solution (excepting the 6.25 mg/L Cu treatment in shoots), while N, P, K, Na, Ca, Mg, B, Mo, and Al concentrations, although showing different patterns, did not reveal any correlation with increasing Cu level. The absolute content of all these metals as well as their net uptake exhibited (excepting Al) its highest values in the 0.25 mg/L Cu treatment. It is suggested that these variations, triggered by excess Cu on root growth, might be explained based on the interaction among Cu‐uptake mechanism(s), plasma membrane‐H⁺ ATPase and root membrane permeability. It is also suggested that Cu affects the N, P, K, Na, Ca, Mg, B, Mo, and Zn concentrations in rice shoots mainly by changing their net uptake rate.  相似文献   

Heartwood extractives from the Amazonian trees <Emphasis Type="Italic">Dipteryx odorata,Hymenaea courbaril</Emphasis>, and <Emphasis Type="Italic">Astronium lecointei</Emphasis> and their antioxidant activities     

Takanori Imai  Sousuke Inoue  Naomi Ohdaira  Yasuyuki Matsushita  Rie Suzuki  Mariko Sakurai  José Manoel Henriques de Jesus  Salete Kiyoka Ozaki  Zenesio Finger  Kazuhiko Fukushima 《Journal of Wood Science》2008,54(6):470-475

Heartwood extracts from Amazonian trees cumaru-ferro (Dipteryx odorata), jatoba (Hymenaea courbaril), and guarita (Astronium lecointei) exhibit antioxidant activities comparable with that of α-tocopherol, a well-known antioxidant. This article reports the characterization of the antioxidant compounds in the extracts of the three heartwoods. Silica gel column chromatography of the cumaru-ferro EtOAc extract yielded (−)-(3R)-7,2′,3′-trihydroxy-4′-methoxyisoflavan and (+)-(3R)-8,2′,3′-trihydroxy-7,4′-dimethoxyisoflavan. Silica gel column chromatography followed by preparative high-performance liquid chromatography of the jatoba EtOAc extract yielded (−)-fisetinidol and (+)-trans-taxifolin. Chemical structures were assigned using electron-ionization mass spectrometry, ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy including nuclear Overhauser effect spectroscopy (NOESY), as well as optical rotation and circular dichroism. Gas chromatography-mass spectrometry demonstrated that the isolated compounds were predominant in the EtOAc extracts. In the guarita EtOAc extract, catechin and gallic acid were identified by comparing their retention times and mass fragmentation patterns with those of authentic samples. Antioxidant activity determined by the 1,1-diphenyl-2-picrylhydrazyl assay demonstrated that all these compounds had activities comparable with that of α-tocopherol. Part of this report was presented at the 57th Annual Meeting of the Japan Wood Research Society, Hiroshima, August 2007  相似文献   

Assessment of insecticidal effects and selectivity of CAPA‐PK peptide analogues against the peach‐potato aphid and four beneficial insects following topical exposure     

Shun‐Hua Gui  Clauvis NT Taning  Kristof De Schutter  Qun Yang  Pengyu Chen  Mohamad Hamshou  Ronald J Nachman  Aniruddha A Pandit  Julian AT Dow  Shireen Davies  Guy Smagghe 《Pest management science》2020,76(10):3451-3458

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Cover Image     

Ana Cristina Silva  Joana Henriques  Eugnio Diogo  Ana Paula Ramos  Helena Bragana 《Forest Pathology》2020,50(1)

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Resistance of 57 greenhouse-grown accessions of Lycopersicon esculentum and three cultivars to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)     

Fabrício Alves Oliveira  Derly José Henriques da Silva  Germano Leão Demolin Leite  Gulab Newandram Jham  Marcelo Picanço 《Scientia Horticulturae》2009

The aim of this study was to evaluate resistance to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) by antixenosis on 57 Lycopersicon esculentum Mill. accessions from the Horticultural Germplasm Bank (HGB) of Universidade Federal de Viçosa and by the three commercial cultivars (Santa Clara, Moneymaker and TOM-601) under greenhouse conditions. A randomized complete block design was used with three replications. Infestations with T. absoluta adults were performed weekly and the following characteristics were evaluated: number of small, large and total mines/leaf and % of leaves mined at days 60, 75 and 90 after planting. Low infestation occurred at days 60 and 75 but at day 90, infestation was sufficient to evaluate insect damage. Based on these data it was concluded that only accessions HGB-674 and HGB-1497 appeared to be the most promising. In addition, to determine possible chemical causes of resistance, hexane extracts were analyzed at day 90 by gas chromatography/mass spectrometry and the major peaks identified by a mass spectral database using similarity index. Nine hydrocarbons, viz., hexadecane, heptadecane, eicosane, tricosane, 2-methyltricosane, tetracosane, hexacosane, octacosane and triacontane were identified in the hexane extracts in many samples. Tricosane, tetracosane and hexacosane presented significant correlations with the leaves mined. Only tricosane presented a negative correlation with the number of small mines (r = −0.28), total number of mines (r = −0.27) and % of leaves mined (r = −0.22). However, tetracosane and hexacosane presented significant positive correlations (r = 0.25 and 0.24, respectively) with the % of leaves mined.  相似文献   

Apoptosis of Granulosa Cells: A Review on the Role of MAPK-signalling modules   总被引：1，自引：0，他引：1  

AT Peter  N Dhanasekaran 《Reproduction in domestic animals》2003,38(3):209-213

Recent studies suggest that ovarian follicular atresia is associated with DNA fragmentation and degeneration of granulosa cells, the hallmark of programmed cell death or apoptosis. Apoptosis of granulosa cells play a major role in follicular atresia. These studies have also demonstrated the involvement of tumour suppressors, apoptotic proteins and survival factors. These factors contribute to the developmental decision as to whether the ovarian follicles mature or undergo atresia. However, the precise temporal and molecular events involved in the apoptotic pathways in this process need to be elucidated. The present report summarizes the role of Jun N‐terminal kinase (JNK), p38 mitogen activated protein kinase (p38 MAPK), and extracellular‐signal regulated kinase (ERK)‐signalling module in the regulation of pro‐ and anti‐apoptotic factors of the granulosa cells in regulating follicular atresia. The findings presented here suggest that the loss of tropic hormone support is translated into the attenuation of Raf‐1‐MAPK/ERK kinase (MEK)‐ERK‐signalling pathway of the granulosa cells and this results in the decreased phosphorylation of the pro‐apoptotic BAD.  相似文献   

Antioxidant and antimutagenic properties of Hibiscus tiliaceus L. methanolic extract     

Rosa RM  Melecchi MI  da Costa Halmenschlager R  Abad FC  Simoni CR  Caramão EB  Henriques JA  Saffi J  de Paula Ramos AL 《Journal of agricultural and food chemistry》2006,54(19):7324-7330

The genus Hibiscus thrives in a variety of climates and produces a diversity of natural compounds with bioactive properties. We have studied the chemical composition and the in vivo antioxidant properties of Hibiscus tiliaceus L. methanolic flower extract, as well as its mutagenic/antimutagenic effects. Vitamin E and some stigmasterol derivatives that might confer an antioxidant effect to the extract were present. Treatment with this extract protected several Saccharomyces cerevisiae strains defective in antioxidant defenses against H2O2 and t-BOOH cytotoxicities, showing a clear antioxidant activity. The effect is the same for all strains used, independent of the antioxidant defense disrupted, suggesting that protection may be due to molecules that act as versatile and wide spectrum nonenzymatic antioxidants, such as vitamins or phytosterols. The extract was not mutagenic in either Salmonella typhimurium or S. cerevisiae and showed a significant antimutagenic action against oxidative mutagens in S. cerevisiae.  相似文献