Evaluation of wood-based panel durability using bending properties after accelerated aging treatments     

Yoichi Kojima  Shigehiko Suzuki 《Journal of Wood Science》2011,57(2):126-133

The durability of wood-based panels was evaluated by comparing the bending properties of panels subjected to five accelerated aging treatments with the bending properties of panels that had experienced 5 years of outdoor exposure in Shizuoka City, Japan. In each accelerated aging treatment, methylene diphenyl diisocyanate-bonded panels showed higher bending retention than phenol formaldehyde (PF)-bonded panels. The bending retentions after six repeated cycles of the JIS-B, APA D-1, and ASTM treatments showed a correspondence of nearly one-to-one in the data for the three different treatments. The Shizuoka City 5-year outdoor exposure test data showed that the bending retentions of all panels decreased with time. In particular, the bending retentions of PF resin-bonded particleboard and oriented strandboard made from aspen were less than 30% and 10% of the original values, respectively, after the 5-year exposure period. The deterioration of the bending properties after the 5-year outdoor exposure in Shizuoka City was the same as that for six repetitions of the ASTM treatment.  相似文献   

Deep ultraviolet light-emitting hexagonal boron nitride synthesized at atmospheric pressure     

Kubota Y  Watanabe K  Tsuda O  Taniguchi T 《Science (New York, N.Y.)》2007,317(5840):932-934

Materials emitting light in the deep ultraviolet region around 200 nanometers are essential in a wide-range of applications, such as information storage technology, environmental protection, and medical treatment. Hexagonal boron nitride (hBN), which was recently found to be a promising deep ultraviolet light emitter, has traditionally been synthesized under high pressure and at high temperature. We successfully synthesized high-purity hBN crystals at atmospheric pressure by using a nickel-molybdenum solvent. The obtained hBN crystals emitted intense 215-nanometer luminescence at room temperature. This study demonstrates an easier way to grow high-quality hBN crystals, through their liquid-phase deposition on a substrate at atmospheric pressure.  相似文献   

Small-Scale Preparation of Fluorescently Labeled Chemical Probes from Marine Cyclic Peptides,Kapakahines A and F     

Rie Kamihira  Yoichi Nakao 《Marine drugs》2021,19(2)

A number of bioactive marine natural products have been isolated so far, but it is still difficult to disclose their modes of action. In this study, we prepared fluorescently labeled chemical probes from the cytotoxic marine cyclic peptides kapakahines A (1) and F (2) to visualize their localization as the first step of the study of their modes of action. We used fluorescent dyes 3a or 3a/b (a 1:1 mixture of 3a and 3b) whose terminal N-hydroxysuccinimide (NHS) group can react with the free amino groups of kapakahines. The fluorescently labeled kapakahine A (Kap A-5-FL, 5a) stained P388 murine leukemia cells and HeLa human cervical cancer cells, while cells treated with fluorescently labeled kapakahine F (Kap F-5-FL, 6a) only weakly stained them. Further analysis of the confocal images of the stained cells with higher magnification (×100) indicated the localization of Kap A-5-FL (5a) in the cells. In this paper, we report the small-scale preparation and a new delivery method of fluorescent probes, as well as the application of these procedures to cell staining.  相似文献   

cDNA cloning of Japanese oyster stress protein homologous to the mammalian 78-kDa glucose regulated protein and its induction by heatshock   总被引：1，自引：0，他引：1  

Yoshihiro Yokoyama  Hisashi Hashimoto  Satoshi Kubota  Akira Kuriyama  Yoichi Ogura  Shoshi Mizuta  Reiji Yoshinaka  Haruhiko Toyohara 《Fisheries Science》2006,72(2):402-409

ABSTRACT:    The 78-kDa glucose regulated protein (GRP78), a member of stress proteins, was cloned from a cDNA library of Japanese oyster Crassostrea gigas . The analysis on Japanese oyster GRP78 clone of approximately 2.6 kb revealed that the entire open reading frame was 1983 bp long and encoded 661 amino acid residues. At the DNA sequence level, the coding region of Japanese oyster GRP78 gene was 72, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71 genes, respectively. Deduced amino acid sequence of Japanese oyster GRP78 was 84, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71, respectively. Japanese oyster GRP78 contained an 18-residue sequence at the N-terminus that exhibits characteristics of a cleavable signal sequence. It also contained an ATPase domain, and a peptide-binding domain in addition to a Lys-Asp-Glu-Leu (KDEL) peptide motif that is involved in determining endoplasmic reticulum localization. Northern blot analysis showed that GRP78 mRNA was induced with heatshock treatment in the oyster tissues.  相似文献   

Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp.     

Yoichi Nakao  Takeru Shiroiwa  Shuhei Murayama  Shigeki Matsunaga  Yasuyuki Goto  Yoshitsugu Matsumoto  Nobuhiro Fusetani 《Marine drugs》2004,2(2):55-62

The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC₅₀ value of 0.2 μg/mL.  相似文献   

Usefulness of cefovecin disk‐diffusion test for predicting mecA gene‐containing strains of Staphylococcus pseudintermedius and clinical efficacy of cefovecin in dogs with superficial pyoderma     

Keita Iyori  Yoichi Toyoda  Kaori Ide  Toshiroh Iwasaki  Koji Nishifuji 《Veterinary dermatology》2013,24(1):162-e36

Background –  Cefovecin has been widely used to treat skin infections in dogs. The relationship of the cefovecin disk‐diffusion test results to the presence of the mecA gene and the clinical efficacy of cefovecin have not been fully evaluated. Hypothesis/Objectives –  To determine the usefulness of an in vitro cefovecin disk‐diffusion test in predicting the presence of the mecA gene in Staphylococcus pseudintermedius, as well as the in vivo efficacy of cefovecin therapy in dogs with superficial pyoderma. Methods –  Twenty‐six S. pseudintermedius strains isolated from 22 dogs with pyoderma were used. In vitro disk‐diffusion test results of cefovecin were compared with agar‐dilution test results, the presence of the mecA gene, and the improvement in clinical scores of dogs with superficial pyoderma at 14 days post treatment. Results –  There was a significant linear correlation (r = ?0.83) between the diameter of the obvious zone of inhibition by disk diffusion and the minimal inhibitory concentration for cefovecin (P < 0.0001). Receiver operating characteristic analysis revealed that zone diameters between 25 and 27 mm exhibited better sensitivity (92.9%) and specificity (100.0%) for detection of strains carrying the mecA gene. The mean improvement in clinical scores in dogs carrying cefovecin‐resistant strains was significantly lower than in dogs carrying cefovecin‐susceptible strains (P < 0.01). Conclusions and clinical importance –  The cefovecin disk‐diffusion test with a cut‐off value estimated in this study was valuable for predicting mecA gene carriage in S. pseudintermedius, as well as the in vivo efficacy of cefovecin therapy in dogs with superficial pyoderma caused by S. pseudintermedius.  相似文献   

Binding effect of cellulose nanofibers in wood flour board     

Yoichi Kojima  Jun Minamino  Akiko Isa  Shigehiko Suzuki  Hirokazu Ito  Rie Makise  Masaki Okamoto 《Journal of Wood Science》2013,59(5):396-401

Wood-based materials are extensively used for residual construction worldwide, especially in Japan. Most wood-based materials are fabricated using adhesives, some of which are not environmentally friendly. As an alternative to chemical adhesives, we explored this issue using nanofiber technology, especially the use of cellulose nanofibers (CNF), as reinforcement in wood flour (WF) board to replace chemical adhesives. We found that CNF could be easily made by pulverization in a ball mill. The physical and mechanical properties of WF board were improved by the three-dimensional binding effects of the CNF.  相似文献   

Nucleotide polymorphisms and the 5′‐UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene     

Masanori KOMATSU  Yuki FUJIMORI  Yoichi SATO  Hiroaki OKAMURA  Seiki SASAKI  Tomohito ITOH  Mitsuo MORITA  Ryoichi NAKAMURA  Toshiaki OE  Masako FURUTA  Junpei YASUDA  Takatoshi KOJIMA  Toshio WATANABE  Takeshi HAYASHI  Aduli E.O. MALAU‐ADULI  Hideaki TAKAHASHI 《Animal Science Journal》2010,81(5):530-550

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International recommendations for training future toxicologic pathologists participating in regulatory-type, nonclinical toxicity studies     

Bolon B  Barale-Thomas E  Bradley A  Ettlin RA  Franchi CA  George C  Giusti AM  Hall R  Jacobsen M  Konishi Y  Ledieu D  Morton D  Park JH  Scudamore CL  Tsuda H  Vijayasarathi SK  Wijnands MV 《Journal of toxicologic pathology》2010,23(3):171-181

The International Federation of Societies of Toxicologic Pathologists (IFSTP) proposes a common global framework for training future toxicologic pathologists who will support regulatory-type nonclinical toxicology studies. Trainees optimally should undertake a scientific curriculum of at least 5 years at an accredited institution leading to a clinical degree (veterinary medicine or medicine). Trainees should then obtain 4 or more years of intensive pathology practice during a residency and/or on-the-job "apprenticeship," at least 2 years of which must be focused on regulatory-type toxicologic pathology topics. Possession of a recognized pathology qualification (i.e., certification) is highly recommended. A non-clinical pathway (e.g., a graduate degree in medical biology or pathology) may be possible if medically trained pathologists are scarce, but this option is not optimal. Regular, lifelong continuing education (peer review of nonclinical studies, professional meetings, reading, short courses) will be necessary to maintain and enhance one's understanding of current toxicologic pathology knowledge, skills, and tools. This framework should provide a rigorous yet flexible way to reliably train future toxicologic pathologists to generate, interpret, integrate, and communicate data in regulatory-type, nonclinical toxicology studies.  相似文献   

Effect of temperature,hypoxia and disinfection with ozonated seawater during somitogenesis on muscular development of the trunk in larval seven‐band grouper,Epinephelus septemfasciatus (Thunberg)          下载免费PDF全文

Susumu Uji  Tohru Suzuki  Takashi Iwasaki  Kazuhisa Teruya  Keiichi Hirasawa  Makoto Shirakashi  Shizumasa Onoue  Yoichi Yamashita  Masaharu Tsuji  Yasushi Tsuchihashi  Koichi Okuzawa 《Aquaculture Research》2015,46(11):2698-2706

The cause of abnormal musculature on both sides of the same myotome in artificially cultured seven‐band grouper Epinephelus septemfasciatus (Thunberg) larvae remains unclear. We evaluated the effect of three factors: changes in temperature (between 22 and 24°C), disinfection with ozonated seawater (0.3 mg TROs L^?1, 1 min), and hypoxia (22 or 14% saturation) during the early (20 h post‐fertilization, hpf) and mid‐ (24 hpf) somite stages on trunk muscular development in larval seven‐band grouper. The thermal regimes and disinfection with ozonated seawater had no effect on muscle development (P > 0.05, G‐test). In contrast, exposure to hypoxia during both the early and mid‐somite stages increased the incidence of abnormal musculature in the trunk (P < 0.01, G‐test). These larvae had abnormal musculature on both sides of the trunk. In addition, the number of abnormal myotomes differed among these larvae. Exposure in the early and mid‐somite stages resulted in the highest frequency of abnormalities in the 11th and 13th myotome respectively. Our results suggest that hypoxia during somitogenesis can lead to abnormal muscle development in the trunk of artificially cultured seven‐band grouper larva.  相似文献