Induced expression of selected plant defence related genes in pot azalea, <Emphasis Type="Italic">Rhododendron simsii</Emphasis> hybrid     

Gil Luypaert  Johan Witters  Johan Van Huylenbroeck  Patrick De Clercq  Jan De Riek  Ellen De Keyser 《Euphytica》2017,213(10):227

A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, β-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 μM MeJA and 0.1 μM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: ‘Nordlicht’, ‘Elien’, ‘Aiko Pink’, ‘Michelle Marie’, ‘Mevrouw Gerard Kint’ and ‘Sachsenstern’. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype ‘Elien’ has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes.  相似文献   

Antioxidant Capacity of Beetroot: Traditional vs Novel Approaches     

Celia Carrillo  Raquel Rey  Marc Hendrickx  María del Mar Cavia  Sara Alonso-Torre 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):266-273

Red beetroot has been ranked among the 10 most potent antioxidant vegetables, although only extraction-based methods have been used to evaluate its total antioxidant capacity. Therefore, the present study aims at comparing the traditional extraction-based method with two more recent approaches (QUENCHER -QUick, Easy, New, CHEap and Reproducible- and GAR -global antioxidant response method), in order to establish their suitability in the case of beetroot. Our results indicate that the total antioxidant capacity of beetroot would be underestimated when using extraction-based procedures, since both QUENCHER and GAR methods resulted in a higher total antioxidant capacity. The effect of a thermal treatment on the total antioxidant capacity of beetroot varies among the methods evaluated and our findings suggest different compounds responsible for the total antioxidant capacity detected in each pre-processing method. Remarkably, the present study demonstrates that the traditional extraction-based method seems useful to screen for (changes in) the “bioavailable” antioxidant potential of the root.  相似文献   

High vegetative compatibility diversity of Cryphonectria parasitica infecting sweet chestnut (Castanea sativa) in Britain indicates multiple pathogen introductions     

A. Pérez-Sierra  P. Romón-Ochoa  C. Gorton  A. Lewis  H. Rees  S. Van Der Linde  J. Webber 《Plant pathology》2019,68(4):727-737

Chestnut blight, caused by Cryphonectria parasitica, was identified in Devon, UK, in December 2016. Intensive surveys detected the disease at further sites in Devon (seven), Berkshire (one), Dorset (one), Derbyshire (four) and a cluster of eight sites in southeast London. Over 570 survey samples were tested, and 227 were positive for C. parasitica by isolation and real-time PCR. A total of 227 isolates were tested for mating type, and 197 screened for vegetative compatibility group (VCG) and compared with VCGs known from mainland Europe. The same isolates were also screened for the presence of Cryphonectria hypovirus 1 (CHV-1). Eleven VCGs were identified within the UK population. Five corresponded to already known European VCGs but six were unique. The European VCGs mainly came from the Devon, Dorset, Berkshire and Derbyshire disease outbreaks, whilst unique VCGs were almost exclusively from the southeast London cluster. Both mating types were detected, but only one mating type was present at each site, with the exception of a single Devon site. Perithecia of C. parasitica were never observed at any site. CHV-1 was found in seven isolates from three different locations and was always subtype-I, which has limited hypovirulence. Therefore, although CHV-1 is associated with C. parasitica at some outbreaks, it probably has limited impact on virulence. The diversity of VCGs and their distribution at outbreak sites, together with findings of CHV-1, suggests C. parasitica has been introduced to the UK multiple times over at least two decades through international plant trade.  相似文献   

Development of enzymes for robust aryloxyphenoxypropionate and synthetic auxin herbicide tolerance traits in maize and soybean crops     

Clayton T Larue  Michael Goley  Lei Shi  Artem G Evdokimov  Oscar C Sparks  Christine Ellis  Andrew M Wollacott  Timothy J Rydel  Coralie E Halls  Brook Van Scoyoc  Xiaoran Fu  Jeffrey R Nageotte  Adewale M Adio  Meiying Zheng  Eric J Sturman  Graeme S Garvey  Marguerite J Varagona 《Pest management science》2019,75(8):2086-2094

  相似文献   

Correction to: Individual based modelling of fish migration in a 2-D river system: model description and case study     

Snyder  Marcía N.  Schumaker  Nathan H.  Ebersole  Joseph L.  Dunham  Jason B.  Comeleo  Randy L.  Keefer  Matthew L.  Leinenbach  Peter  Brookes  Allen  Cope  Ben  Wu  Jennifer  Palmer  John  Keenan  Druscilla 《Landscape Ecology》2019,34(5):1197-1197

Landscape Ecology - In the original publication of the article, the third author name has been misspelt. The correct name is given in this Correction. The original version of this article was revised.  相似文献   

Root-growth response of winter barley to subsoiling     

G. Ide  G. Hofman  C. Ossemerct  M. Van Ruymbeke 《Soil & Tillage Research》1984,4(5):419-431

Root growth of winter barley was studied on 2 Belgian silt loam soils after subsoiling to 60 cm depth. In the first field (well-drained) rooting depth increased significantly after removal of a plough pan. At the flowering stage, total root weight in the soil layers between 25–50 cm and 50–75 cm was 125% and 325%, respectively, higher on the subsoiled plots than on the control. The P₂O₅ and K₂O concentrations in the roots were significantly increased by 20% and 40%, respectively. In the second field (imperfectly drained) with initially relatively high bulk densities in the subsoil, the influence of subsoiling was negligible. In this study, the clearly-shown root-growth retardation that resulted in a lower yield on the control, seemed to be caused by the high penetration resistance in the plough sole (> 3MPA) rather than poor aeration status induced by the high bulk densities.  相似文献   

Evaluation of the simultaneous effects of processing parameters on the iron and zinc solubility of infant sorghum porridge by response surface methodology     

Kayodé AP  Nout MJ  Bakker EJ  Van Boekel MA 《Journal of agricultural and food chemistry》2006,54(12):4253-4259

The purpose of this study was to improve the micronutrient quality of indigenous African infant flour using traditional techniques available in the region. Response surface methodology was used to study the effect of duration of soaking, germination, and fermentation on phytate and phenolic compounds (PC), pH, viscosity, and the in vitro solubility (IVS) of iron and zinc in infant sorghum flour. The phytate and the PC concentrations of the flour were significantly modified as a result of the duration of germination and fermentation and their mutual interaction. These modifications were accompanied by a significant increase in % IVS Zn after 24 h of sprouting. Except for the interaction of soaking and fermentation, none of the processing parameters exerted a significant effect on the % IVS Fe. The viscosity of the porridge prepared with the flour decreased significantly with the duration of germination, making it possible to produce a porridge with high energy and nutrient density. The use of germination in combination with fermentation is recommended in the processing of cereals for infant feeding in developing countries.  相似文献   

Influence of storage practices on acrylamide formation during potato frying     

De Wilde T  De Meulenaer B  Mestdagh F  Govaert Y  Vandeburie S  Ooghe W  Fraselle S  Demeulemeester K  Van Peteghem C  Calus A  Degroodt JM  Verhé R 《Journal of agricultural and food chemistry》2005,53(16):6550-6557

A number of parameters linked to storage of potatoes were evaluated with regard to their potential to influence the acrylamide formation in French fries. Acrylamide, which is a potential human carcinogen, is reported to be formed during the frying of potatoes as a result of the reactions between asparagine and reducing sugars. This study was conducted using three potato varieties (Bintje, Ramos, and Saturna) typically used in Belgium, The Netherlands, and the northern part of France for French fry and crisp production. Saturna, mainly used in crisp production, appeared to be the least susceptible for acrylamide formation during frying. Especially storage at low temperatures (4 degrees C) compared to storage at 8 degrees C seemed to enhance acrylamide formation due to a strong increase in reducing sugars caused by low-temperature storage. Because of the reversible nature of this physiological reaction, it was possible to achieve a significant reduction of the reducing sugars after a reconditioning of the cold-stored potatoes for 3 weeks at 15 degrees C. All changes in acrylamide concentrations could mainly be explained by the reducing sugar content of the potato (R2 = 0.84, n = 160). This means that, by ensuring a low reducing sugar content of the potato tuber, the risk for acrylamide formation will largely be reduced. Finally the use of a sprout inhibitor did not influence the composition of the potato, and thus acrylamide formation was not susceptible to this treatment.  相似文献   

1：25万土壤及地形数据库及指标体系研究     

包亮宇振荣  门明新VanEnglen 《农业工程学报》2004,20(4):259-263

根据土壤及地形(SOTER)数据库基本原理和方法建立1：25万SOTER数据库,重点研究和修正大中比例尺SOTER主地形体划分指标体系,提出海拔高度分组指标、坡度分组指标和地势起伏强度分组指标,并利用上述3个指标组合计算出样区SOTER主地形体组分(中坡度丘陵、低坡度山体台地、山谷、中坡度山体、山前倾斜平原和冲洪积平原),以进一步发展和完善SOTER数据库理论方法体系。同时,SOTER数据库的建立使原有的历次土壤普查、国土资源详查的资料信息化,使之服务于土地资源的评价、管理与保护。  相似文献   

Development of an indirect competitive ELISA for flumequine residues in raw milk using chicken egg yolk antibodies   总被引：3，自引：0，他引：3  

Van Coillie E  De Block J  Reybroeck W 《Journal of agricultural and food chemistry》2004,52(16):4975-4978

To detect flumequine in raw milk, an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed. By carbodiimide conjugation, flumequine was conjugated to cationized bovine serum albumin (cBSA-flumequine) and to cationized ovalbumin (cOVA-flumequine). For the immunization of chickens, cBSA-flumequine was used, which allowed the isolation of specific chicken egg yolk immunoglobulins (IgY) for flumequine. As the coating antigen in the immunoassay, cOVA-flumequine was used. In the indirect competitive assay, standard flumequine was incubated together with the anti-flumequine antibodies. The antibody by which the lowest concentration of free flumequine that gives 50% inhibition of binding (IC50) was found in aqueous dilution was further tested for the applicability to detect flumequine in raw milk. An IC50 level in milk was reached that was about 5 times lower than in aqueous solution. So flumequine can be detected directly in raw milk at maximum residue level (50 microg/kg). No cross-reactivity was noticed with various related quinolones.  相似文献