全文获取类型
收费全文 | 2558篇 |
免费 | 154篇 |
国内免费 | 231篇 |
专业分类
林业 | 159篇 |
农学 | 178篇 |
基础科学 | 61篇 |
243篇 | |
综合类 | 1318篇 |
农作物 | 202篇 |
水产渔业 | 83篇 |
畜牧兽医 | 382篇 |
园艺 | 186篇 |
植物保护 | 131篇 |
出版年
2024年 | 16篇 |
2023年 | 53篇 |
2022年 | 126篇 |
2021年 | 100篇 |
2020年 | 116篇 |
2019年 | 92篇 |
2018年 | 85篇 |
2017年 | 90篇 |
2016年 | 69篇 |
2015年 | 104篇 |
2014年 | 124篇 |
2013年 | 144篇 |
2012年 | 172篇 |
2011年 | 196篇 |
2010年 | 226篇 |
2009年 | 214篇 |
2008年 | 222篇 |
2007年 | 184篇 |
2006年 | 161篇 |
2005年 | 135篇 |
2004年 | 100篇 |
2003年 | 66篇 |
2002年 | 48篇 |
2001年 | 33篇 |
2000年 | 50篇 |
1999年 | 11篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1991年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2943条查询结果,搜索用时 358 毫秒
21.
本研究以感(岷山红三叶)、抗(澳大利亚红三叶品种♀Sensation×Renegade♂杂交新品系“甘农PR1”)白粉病红三叶材料为父母本杂交并种植成苗经人工接菌后筛选出抗、感白粉病的F1群体为作图群体,利用AFLP标记构建红三叶高密度遗传图谱,并利用区间作图法对抗白粉病QTL进行了定位分析,可以为红三叶抗白粉病基因克隆和转基因等分子辅助育种奠定基础。结果表明,149个AFLP标记构建得到的遗传图谱包含7个连锁群(LG1,LG2,LG3,LG4,LG5,LG6和LG7),遗传图谱的总距离为640.5 cM。其中,LG1连锁群的遗传距离(140.6 cM)和标记间平均距离(9.4 cM)均最大;LG4连锁群的遗传距离(55.2 cM)和标记间平均距离(1.8 cM)最小。应用区间作图法对红三叶抗白粉病基因进行QTL分析定位,共检测到5个抗白粉病相关QTL位点(qrp-1,qrp-2,qrp-3,qrp-4和qrp-5),其中qrp-1、qrp-2、qrp-3和qrp-4位于LG4连锁群上,qrp-5位于LG5连锁群上。5个QTL位点对抗白粉病的贡献率为29%~90%,qrp-1对红三叶白粉病抗性的贡献率最大(90%),为主效QTL。 相似文献
22.
23.
WANG Jing-jing ZHANG Xue-mei ZHAO Hong-ling DONG Cheng-hong PU Jing LIAO Yun WANG Li-chun NA Rui-xiong LIU Long-ding ZHANG Ying YANG Li-xian WANG Qing-ling SHEN Dong WANG Xi LI Qi-han 《园艺学报》2011,27(7):1249-1256
AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future. 相似文献
24.
粉碎机是饲料生产的主要设备之一。粉碎效率的高低,设备是否运转正常直接影响饲料生产的质量、产量及饲料生产的综合成本。同时也影响饲料内在的质量和动物的饲养效果。现针对只购买单台粉碎机并自行安装风网的用户提几点建议:1.粉碎机的选型粉碎机选型时,要考虑到产量需求、原料的粒度、水分、原料的可碎性、原料的吸湿性和原料对温度的敏感性等因素。同时还要考虑后道工序对粉碎粒度的要求。 相似文献
25.
LIU Cui-cui GUO Xiao-hong CAO Guo-qing GAO Peng-fei PU Zhong-de NIU Jiao-yan ZHANG Qi LI Meng JIN Yu-shu LI Bu-gao 《中国畜牧兽医》2015,42(10):2726-2731
The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs. 相似文献
27.
[Objective] This study aimed to examine indicative roles of texture representing soil organic carbon presence and variability subsequent to cultivation under cold temperate climates with seasonal freeze-thaw events.[Method] Three chronosequences were selected for paired comparisons.Soil samples were collected at six depths with a 10 cm increment.Analysis of variance with general linear model and regression was performed for statistical analysis.[Result] In seasonally frozen soils where fragmentation of macroaggregates was stimulated,soil organic carbon level was positively associated with clay + silt proportion due to a wider textural range,better than sole clay content.Exponential function better fitted the experimental data to present progressively increased effectiveness of clay + silt content in maintaining carbon.Clay content explained 12%-41% and 14%-43% of variation via linear and exponential functions,respectively.Accordingly,clay + silt content explained 47%-65% and 46%-70%.[Conclusion] Texture reflected soil organic carbon occurrence as consequences of reclamation.For seasonally frozen soils with wider textural ranges,it is robust to adapt clay + silt content as dependent variable and exponential function.The generated algorithms provided an available pathway to estimate soil organic carbon losses following cultivation and to evaluate soil fertility. 相似文献
28.
AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury. 相似文献
29.
30.