CRISPR-Cas Technology in Plant Science     

Peter M. Rogowsky 《Potato Research》2017,60(3-4):353-360

CRISPR-Cas technology has raised considerable interest among plant scientists, both in basic science and in plant breeding. Presently, the generation of random mutations at a predetermined site of the genome is well mastered, just like the targeted insertion of transgenes, although both remain restricted to species or genotypes amenable for plant transformation. On the other hand, true genome editing, i.e. the deliberate replacement of one or several nucleotides of the genome in a predetermined fashion, is limited to some rather particular examples that generally concern genes allowing positive selection, for example tolerance to herbicides. Therefore, further technological developments are necessary to fully exploit the potential of genome editing in enlarging the gene pool beyond the natural variability available in a given species. In principle, the technology can be applied to any quality related, agronomical or ecological trait, under the condition of upstream knowledge on the genes to be targeted and the precise modifications necessary to improve alleles. Published proof of concepts concern a wide range of agronomical traits, the most frequent being disease resistance, herbicide tolerance and the biochemical composition of harvested products. The regulatory status of the plants obtained by CRISPR-Cas technology raises numerous questions, in particular with regard to the plants that carry in their genomes the punctual modifications caused by the presence of the Cas9 nuclease but not the nuclease itself. Without clarification by the competent authorities, CRISPR-Cas technology would continue to be a powerful tool in functional genomics, but its potential in plant breeding would remain untapped.  相似文献   

First transgenic trait for control of plant bugs and thrips in cotton     

Waseem Akbar  Anilkumar Gowda  Jeffrey E Ahrens  Jason W Stelzer  Robert S Brown  Scott L Bollman  John T Greenplate  Jeffrey Gore  Angus L Catchot  Gus Lorenz  Scott D Stewart  David L Kerns  Jeremy K Greene  Michael D Toews  David A Herbert  Dominic D Reisig  Gregory A Sword  Peter C Ellsworth  Larry D Godfrey  Thomas L Clark 《Pest management science》2019,75(3):867-877

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Enhanced metabolism causes reduced flufenacet sensitivity in black‐grass (Alopecurus myosuroides Huds.) field populations     

Rebecka Dücker  Peter Zllner  Evlampia Parcharidou  Susanne Ries  Lothar Lorentz  Roland Beffa 《Pest management science》2019,75(11):2996-3004

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The spread of the fall armyworm Spodoptera frugiperda in Africa—What should be done next?     

Feldmann  Falko  Rieckmann  Ute  Winter  Stephan 《植物病害和植物保护杂志》2019,126(2):97-101

Journal of Plant Diseases and Protection - The following text summarizes the different perspectives of presenters participating in the section Plant Protection in the Tropics and Subtropics, 61st...  相似文献   

Three dimensional (3D) spatial metrics for objects     

Kedron  Peter  Zhao  Yun  Frazier  Amy E. 《Landscape Ecology》2019,34(9):2123-2132

Landscape Ecology - The increasing availability of lidar data and structure from motion processing techniques is moving pattern metric research toward the development of three-dimensional (3D)...  相似文献   

Correction to: Individual based modelling of fish migration in a 2-D river system: model description and case study     

Snyder  Marcía N.  Schumaker  Nathan H.  Ebersole  Joseph L.  Dunham  Jason B.  Comeleo  Randy L.  Keefer  Matthew L.  Leinenbach  Peter  Brookes  Allen  Cope  Ben  Wu  Jennifer  Palmer  John  Keenan  Druscilla 《Landscape Ecology》2019,34(5):1197-1197

Landscape Ecology - In the original publication of the article, the third author name has been misspelt. The correct name is given in this Correction. The original version of this article was revised.  相似文献   

Identifying climate refugia for 30 Australian rainforest plant species,from the last glacial maximum to 2070     

Das  Sourav  Baumgartner  John B.  Esperon-Rodriguez  Manuel  Wilson  Peter D.  Yap  Jia-Yee S.  Rossetto  Maurizio  Beaumont  Linda J. 《Landscape Ecology》2019,34(12):2883-2896

Landscape Ecology - Climate refugia—areas that remain suitable for species during periods of climate disruption—have played an important role in species persistence over time....  相似文献   

基于电磁阀的喷嘴直接注入式农药喷洒系统   总被引：2，自引：0，他引：2  

蔡祥  Walgenbach Martin  Doerpmund Malte  Schulze Lammers Peter  孙宇瑞 《农业机械学报》2013,44(6):69-72,200

设计了一种喷嘴直接注入式农药喷洒系统。通过与传统喷雾机械比较,简述了喷嘴直接注入式喷雾系统的结构和喷雾执行单元。为了实现农药定量注入,设计一种基于电磁原理的快速响应阀,并论述其工作原理和闭环控制方式。通过试验测试了喷雾执行单元的喷雾特性和喷杆组内各喷雾单元的一致性。结果表明在一定喷洒速率范围内,喷雾执行单元的喷水量和水压强存在多项式关系,农药注入量和PWM信号的高电平时间存在指数关系,其决定系数均高于0.9,并且同一喷杆组内6个喷雾单元的喷雾效果大体一致。  相似文献   

Isozyme studies of genetic diversity and evolution in Hippophae   总被引：4，自引：0，他引：4  

Yingmou Yao  Peter M.A. Tigerstedt 《Genetic Resources and Crop Evolution》1993,40(3):153-164

Summary To provide information on the genetic variation, differentiation and evolution in Hippophae seed samples of 25 populations from China, Finland and Russia were electrophoretically analyzed. Of six loci investigated, four were good genetic markers for identifying species and subspecies. The percentages of polymorphic loci per population were 40.3% and 62.5% at 0.95 and 0.99 polymorphic criteria respectively. The mean number of alleles per locus per population was 2.1. Total genetic diversity in the material was 0.4614. Genetic diversity partitioning showed that there was a large amount of diversity residing within geographical populations (0.1354), between subspecies within species (0.1046) and between species (0.2566) but not between geographical populations (0.0114). There were nearly twice as many negative fixation indices as positive ones in Hippophae populations. The phylogenetic tree agreed very well with botanic classifications of the species and subspecies and their geographical distributions, and quantitatively presented the genetic relationships of 25 populations. A detailed view of the evolutionary stages in Hippophae showed clearly a general decline of similarity as evolutionary divergence continued, which further explained the evolution process in Hippophae.  相似文献   

Response of alluvial fan systems to the late Pleistocene to Holocene climatic transition: contrasts between the margins of pluvial Lakes Lahontan and Mojave, Nevada and California, USA   总被引：1，自引：0，他引：1  

Adrian M. Harvey  Peter E. Wigand  Stephen G. Wells 《CATENA》1999,36(4):53

Dated shorelines of late Pleistocene pluvial Lakes Lahontan (Great Basin Desert, northwest Nevada) and Mojave (Mojave Desert, eastern California) provide timelines for the assessment of alluvial fan sedimentation at the lake margins during the late Pleistocene to early Holocene. Two sets of alluvial fan systems have been mapped: the Stillwater fans, feeding Lake Lahontan; and the Zzyzx fans, feeding Lake Mojave. Their contrasting morphologies suggest different responses of the two fan systems to late Pleistocene to early Holocene climatic change. At the time the Stillwater fan systems underwent minimal sedimentation, with the catchment hillslopes apparently stable. The Zzyzx fans experienced major changes in water and sediment supply from the catchment hillslopes. There was a major phase of hillslope debris-flow activity, followed by fanhead trenching and distal fan progradation. Both areas were wetter and colder in the late Pleistocene than they are today, but during the transition to the Holocene the Zzyzx area was more likely to experience intense rains associated with the monsoonal penetration of warm moist tropical air into the Southwest. Vegetation reconstructions for the late Pleistocene to the early Holocene suggest that catchment hillslopes in the Mojave supported a desert shrub vegetation, but those in the Stillwaters supported juniper woodland and grasses at low elevations and pine at higher elevations. Contrasts in hillslope vegetation cover together with storm activity may account for the different responses of the alluvial fans to climatic change during the Pleistocene to Holocene climatic transition. After the falls in lake levels of Lakes Lahontan and Mojave in the early Holocene, both areas underwent aridification, resulting in reductions in hillslope vegetation cover. Increased storm runoff led to fanhead trenching and distal progradation of the alluvial fans. Variations in fan style at that time may relate primarily to base-level conditions resulting from different gradients on the exposed lake shores.  相似文献