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71.
Oulkar DP Banerjee K Patil SH Upadhyay AK Taware PB Deshmukh MB Adsule PG 《Pest management science》2009,65(2):183-188
BACKGROUND: This work was undertaken to determine the preharvest interval (PHI) of buprofezin to minimize its residues in grapes and thereby ensure consumer safety and avoid possible non‐compliance in terms of residue violations in export markets. Furthermore, the residue dynamics in three grapevine soils of India was explored to assess its environmental safety. RESULTS: Residues dissipated following non‐linear two‐compartment first + first‐order kinetics. In grapes, the PHI was 31 days at both treatments (312.5 and 625 g a.i. ha?1), with the residues below the maximum permissible intake even 1 h after foliar spraying. Random sampling of 5 kg comprising small bunchlets (8–10 berries) collected from a 1 ha area gave satisfactory homogeneity and representation of the population. A survey on the samples harvested after the PHI from supervised vineyards that received treatment at the recommended dose showed residues below the maximum residue limit (MRL) of 0.02 mg kg?1 applicable for the European Union. In soil, the degradation rate was fastest in clay soil, followed by sandy loam and silty clay, with a half‐life within 16 days in all the soils. CONCLUSION: The recommendation of the PHI proved to be effective in minimizing buprofezin residues in grapes. Thus, this work is of high practical significance to the domestic and export grape industry of India to ensure safety compliance in respect of buprofezin residues, keeping in view the requirements of international trade. Copyright © 2008 Society of Chemical Industry 相似文献
72.
Bibek Ranjan Shome Mani Bhuvana Susweta Das Mitra Natesan Krithiga Rajeswari Shome Dhanikachalam Velu Apala Banerjee Sukhadeo B. Barbuddhe Krishnamshetty Prabhudas Habibar Rahman 《Tropical animal health and production》2012,44(8):1981-1992
Streptococci are one among the major mastitis pathogens which have a considerable impact on cow health, milk quality, and productivity. The aim of the present study was to investigate the occurrence and virulence characteristics of streptococci from bovine milk and to assess the molecular epidemiology and population structure of the Indian isolates using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Out of a total of 209 bovine composite milk samples screened from four herds (A–D), 30 Streptococcus spp. were isolated from 29 milk samples. Among the 30 isolates, species-specific PCR and partial 16S rRNA gene sequence analysis identified 17 Streptococcus agalactiae arising from herd A and 13 Streptococcus uberis comprising of 5, 7, and 1 isolates from herds B, C, and D respectively. PCR based screening for virulence genes revealed the presence of the cfb and the pavA genes in 17 and 1 S. agalactiae isolates, respectively. Similarly, in S. uberis isolates, cfu gene was present in six isolates from herd C, the pau A/skc gene in all the isolates from herds B, C, and D, whereas the sua gene was present in four isolates from herd B and the only isolate from herd D. On MLST analysis, all the S. agalactiae isolates were found to be of a novel sequence type (ST), ST-483, reported for the first time and is a single locus variant of the predicted subgroup founder ST-310, while the S. uberis isolates were found to be of three novel sequence types, namely ST-439, ST-474, and ST-475, all reported for the first time. ST-474 was a double locus variant of three different STs of global clonal complex ST-143 considered to be associated with clinical and subclinical mastitis, but ST-439 and ST-475 were singletons. Unique sequence types identified for both S. agalactiae and S. uberis were found to be herd specific. On PFGE analysis, identical or closely related restriction patterns for S. agalactiae ST-483 and S. uberis ST-439 in herds A and B respectively, but an unrelated restriction pattern for S. uberis ST-474 and ST-475 isolates from herds D and C respectively, were obtained. This signifies that the isolates of particular ST may exhibit related PFGE patterns suggesting detection of a faster molecular clock by PFGE than MLST. Since all the isolates of both the species belonged to novel sequence types, their epidemiological significance in global context could not be ascertained, however, evidence suggests that they have uniquely evolved in Indian conditions. Further research would be useful for understanding the role of these pathogens in bovine sub-clinical mastitis and implementing effective control strategies in India. 相似文献
73.
The present study was designed to determine the correlations among Anaplasma marginale parasitemia and markers of oxidative stress in crossbred calves. Blood was collected from 11 crossbred calves infected with
A. marginale along with 11 healthy crossbred calves as controls for determination of hematology and oxidative stress indicators. Percentage
of parasitemia in infected calves varied from 0.8% to 6.0%. The values of hematological indicators and antioxidant enzymes
were decreased, whereas erythrocytic lipid peroxidation (LPO) and plasma nitrate (NO) level were significantly (p < 0.05) augmented in A. marginale-infected animals over healthy group. Parasitemia was positively correlated (p < 0.01) with erythrocytic LPO and plasma NO and negatively correlated (p < 0.01) with hematological indicators and antioxidant enzymes. In addition, erythrocytic LPO was negatively correlated (p < 0.01) with the hemoglobin, erythrocyte count, and packed cell volume. From the present study, it can be concluded that
anaplasmosis in crossbred calves is associated with a parasitic load-dependent oxidative damage as indicated by poor antioxidant
status and enhanced oxidative stress, which are contributed to severe anemia. 相似文献
74.
Rad R Rad L Wang W Cadinanos J Vassiliou G Rice S Campos LS Yusa K Banerjee R Li MA de la Rosa J Strong A Lu D Ellis P Conte N Yang FT Liu P Bradley A 《Science (New York, N.Y.)》2010,330(6007):1104-1107
75.
A study on evapotranspiration from potato fields was conducted in the Lower Gangetic Plains of India. The input data required for the CROPWAT irrigation management model was collected, and evapotranspiration (ET) and irrigation water requirement (IWR) for potato crops were calculated using the model. Firstly, the CROPWAT model was validated by comparing simulated crop evapotranspiration (SET) with actual ET calculated through the field water balance method. Thereafter, SET and IWR for nine locations in the lower Gangetic plains of India were calculated for the period from 1996–1997 to 2008–2009, for the current situation (using 20-year-average weather data of the stations), and for elevated thermal conditions, i.e. considering 2 and 3 °C increases over the current temperature. The future change in IWR for potato up to 2050 was also calculated considering the projected climatic scenario generated by the PRECIS model. The CROPWAT calculated IWR values showed an increasing, though not statistically significant, trend in requirement of irrigation water for potato across the nine locations during the period from 1996–1997 to 2008–2009. At a temperature increase of 2 °C over normal, the mean SET of potato would increase by 0.06 mm per day and the average IWR would be 6.0 mm per season more. If the mean temperature would be 3 °C more than normal, the SET would be 0.16 mm day?1 higher and the IWR 16.6 mm. Also based on the projected climatic scenario generated by the PRECIS model, the future SET up to 2050 showed an increasing trend. The present study indicates increasing demand for irrigation water, which may significantly affect the agricultural scenario in the region. 相似文献
76.
Triploblastic animals more than 1 billion years ago: trace fossil evidence from india 总被引:3,自引:0,他引:3
Some intriguing bedding plane features that were observed in the Mesoproterozoic Chorhat Sandstone are biological and can be interpreted as the burrows of wormlike undermat miners (that is, infaunal animals that excavated tunnels underneath microbial mats). These burrows suggest that triploblastic animals existed more than a billion years ago. They also suggest that the diversification of animal designs proceeded very slowly before the appearance of organisms with hard skeletons, which was probably the key event in the Cambrian evolutionary explosion, and before the ecological changes that accompanied that event. 相似文献
77.
Determination of critical limits of soil nutrients for use in optimizing fertilizer rates for fish ponds in red, lateritic soil zones 总被引:1,自引:0,他引:1
Mesocosms containing lateritic soil from West Bengal, India and de-ionized water were treated with different amounts of nitrogen (N), phosphorus (P), and potassium (K) fertilizers. Concentrations of available N, P, and K in soils and gross primary productivity were measured for the mesocosms. Critical concentrations of the three nutrients were estimated by the graphical method of Cate and Nelson that has been used widely for establishing critical soil nutrient concentrations in fertilization research on terrestrial crop plants. Critical concentrations of the three nutrients were: available N, 200 mg kg−1; available P, 13 mg kg−1; available K, 80 mg kg−1. Ponds sited in red, lateritic soil in West Bengal were treated monthly with 20, 15, and 3 kg ha−1 application−1 of N, P, and K, respectively. There was an increase in gross primary productivity when the critical concentrations of available N, P, and K established in the mesocosm study were attained in the pond soils. Fish production in fertilized ponds is positively correlated with gross primary productivity. Thus, the concept of critical nutrient concentrations in soils could be a valuable tool in pond fertilization research. 相似文献
78.
79.
Use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. This has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. This study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. Ten pathogenic bacterial species isolated from shrimp, Penaeus monodon, and Artemia cysts were tested for susceptibility to indigenous marine Bacillus subtilis AB65, Bacillus pumilus AB58, Bacillus licheniformis AB69 and compared with oxytetracycline, chloramphenicol, gentamicin and bacitracin, which are common antibiotics used in Asian aquaculture. The Bacillus spp. were isolated from the local marine environment for bioremediation use in shrimp hatcheries and were proven to reduce total ammonium nitrogen. The pathogenic bacterial isolates were 90% susceptible to B. subtilis AB65, 70% susceptible to B. pumilus AB58 and B. licheniformis AB69 and 100% susceptible to oxytetracycline, chloramphenicol and gentamicin but only 40% to bacitracin. Two representative isolates of the vibrio group, Vibrio alginolyticus VaM11 and Vibrio parahaemolyticus VpM1, when tested for competitive exclusion by a common broth method using the marine Bacillus spp., showed decreased viable counts from 10(8) to 10(2) cfu mL(-1). The results suggest that the action of the marine bacteria appears to be significant in protecting the host shrimp against pathogenic bacteria. In addition to the alternative use of antibiotics, the selected marine bacteria had additional bioremediation properties of reducing ammonia. 相似文献
80.