Pesticide contamination of the upper Elbe River and an adjacent floodplain area     

Karlsson  Anneli S.  Lesch  Michael  Weihermüller  Lutz  Thiele  Björn  Disko  Ulrich  Hofmann  Diana  Vereecken  Harry  Spielvogel  Sandra 《Journal of Soils and Sediments》2020,20(4):2067-2081

Journal of Soils and Sediments - Pesticide contamination of river waters is a global problem, and therefore, authorities regularly monitor the water quality status. Especially, flood events might...  相似文献   

Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle   总被引：1，自引：0，他引：1  

Charlier J  De Meulemeester L  Claerebout E  Williams D  Vercruysse J 《Veterinary parasitology》2008,153(1-2):44-51

The objectives of this study were (1) to evaluate available coprological and serological tests for detection of Fasciola hepatica infection in field conditions, (2) to investigate if the season when samples were collected affects the interpretation of the test results, and (3) to evaluate if the test results are associated with the level of infection. During weekly visits to an abattoir, the whole liver, a rectal faecal sample and a blood sample were collected from 100 cows in two seasons each ("spring"=February-May 2006 and "autumn"=October-December 2006). A sedimentation-flotation technique on 4g (SF 4g) or 10g (SF 10g) of faeces, a copro-antigen ELISA and two indirect serum F. hepatica ELISAs (excretory-secretory (ES) and Pourquier ELISA) were performed and the test results were compared with the presence of infection and worm counts at liver necropsy. Over both seasons the sensitivity (Se) and specificity (Sp) were for the SF 4g 43% and 100%, for the SF 10g 64% and 93%, for the copro-antigen ELISA 94% and 93%, for the ES ELISA 87% and 90% and for the Pourquier ELISA 88% and 84%. Significant between-season differences (P<0.05) were observed in the sensitivities of the two serological ELISAs: whereas the Pourquier ELISA had a higher sensitivity in spring than in autumn, the opposite was true for the ES ELISA. There were no significant between-season differences in the specificity for any of the tests. The test results of the SF 4g, copro-antigen ELISA and ES ELISA were associated with the level of infection of the animal. Given a positive test result of the SF 4g it is at least 11 times more likely that the animal is carrying a heavy infection (>10 flukes) than that is free of infection or lightly infected (< or = 10 flukes). Weak ( approximately 0.3) and moderate ( approximately 0.6) correlations were observed within infected animals of level of infection with ES and copro-antigen ELISA results, respectively.  相似文献   

Comparison of two inoculation methods for Microsporum canis culture using the toothbrush sampling technique     

Diana Di Mattia  Alessandra Fondati  Moira Monaco  Mario Pasquetti  Andrea Peano 《Veterinary dermatology》2019,30(1):60-e17

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The effects of starter microbiota and the early life feeding of medium chain triglycerides on the gastric transcriptome profile of 2-or 3-week-old cesarean delivered piglets     

Paolo Trevisi  Davide Priori  Vincenzo Motta  Diana Luise  Alfons J.M.Jansman  Sietse-Jan Koopmans  Paolo Bosi 《畜牧与生物技术杂志(英文版)》2018,(1)

Background: The stomach is an underestimated key interface between the ingesta and the digestive system, affecting the digestion and playing an important role in several endocrine functions. The quality of starter microbiota and the early life feeding of medium chain triglycerides may affect porcine gastric maturation. Two trials(T1, T2) were carried out on 12 and 24 cesarean-delivered piglets(birth, d0), divided over two microbiota treatments, but slaughtered and sampled at two or three weeks of age, respectively. All piglets were fed orally: sow serum(T1) or pasteurized sow colostrum(T2) on d0; simple starter microbiota(Lactobacillus amylovorus, Clostridium glycolicum and Parabacteroides spp.)(d1-d3); complex microbiota inoculum(sow diluted feces, CA) or a placebo(simple association, SA)(d3-d4) and milk replacer ad libitum(d0-d4). The The T1 piglets and half of the T2 piglets were then fed a moist diet(CTRL); the remaining half of the T2 piglets were fed the CTRL diet fortified with medium chain triglycerides and 7% coconut oil(MCT). Total m RNA from the oxyntic mucosa was analyzed using Affymetrix?Porcine Gene array strips. Exploratory functional analysis of the resulting values was carried out using Gene Set Enrichment Analysis.Results: Complex microbiota upregulated 11 gene sets in piglets of each age group vs. SA. Of these sets, 6 were upregulated at both ages, including the set of gene markers of oxyntic mucosa. In comparison with the piglets receiving SA, the CA enriched the genes in the sets related to interferon response when the CTRL diet was given while the same sets were impoverished by CA with the MCT diet.Conclusions: Early colonization with a complex starter microbiota promoted the functional maturation of the oxyntic mucosa in an age-dependent manner. The dietary fatty acid source may have affected the recruitment and the maturation of the immune cells, particularly when the piglets were early associated with a simplified starter microbiota.  相似文献   

Diagnostic and prognostic utility of surface electrocardiography in cats with left ventricular hypertrophy     

G. Romito  C. Guglielmini  M.O. Mazzarella  M. Cipone  A. Diana  B. Contiero  M. Baron Toaldo 《Journal of Veterinary Cardiology》2018,20(5):364-375

Objectives

To assess the ability of electrocardiography (ECG) to predict left ventricular hypertrophy (LVH) in the cat and to investigate the prognostic value of selected ECG variables in cats with LVH.

Animals

Fifty-seven privately owned cats: 22 clinically healthy cats and 35 cats with LVH.

Material and methods

This is a clinical cohort study. Echocardiographic diagnosis and surface ECG were available. Electrocardiography analysis included rhythm diagnosis and specific electrocardiographic measurements. In cats with LVH, cause of death and outcome data were recorded and analyzed using Kaplan–Meier curves.

Results

The presence of arrhythmia had sensitivity and specificity of 31% and 100%, respectively, for identifying LVH. Among ECG measurements, duration of QT interval (QT) and QT interval corrected for heart rate (QTc) was statistically different between healthy cats and cats with LVH (p = 0.007). Overall, the most accurate cutoffs to identify LVH were QT > 170 ms (sensitivity and specificity 48.3% and 91%, respectively) and QTc > 188 ms (sensitivity and specificity 62% and 77%, respectively). In healthy cats, the highest QT and QTc values were 180 ms and 200 ms, respectively. Mean survival time was 58 days and indeterminable for cats with QT > 180 ms and QT ≤ 180 ms, respectively (p = 0.042) and 125 days and indeterminable for cats with QTc > 200 ms and QTc ≤ 200 ms, respectively (p = 0.017).

Conclusions

Arrhythmias as well as prolonged QT and QTc are useful ECG parameters in identifying LVH and predicting survival in affected cats.  相似文献   

Oral soft tissue anatomy in the dog and cat     

Eubanks DL 《Journal of veterinary dentistry》2007,24(2):126-129

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From Natural Xanthones to Synthetic C-1 Aminated 3,4-Dioxygenated Xanthones as Optimized Antifouling Agents     

Diana I. S. P. Resende  Joana R. Almeida  Sandra Pereira  Alexandre Campos  Agostinho Lemos  Jeffrey E. Plowman  Ancy Thomas  Stefan Clerens  Vitor Vasconcelos  Madalena Pinto  Marta Correia-da-Silva  Emília Sousa 《Marine drugs》2021,19(11)

Biofouling, which occurs when certain marine species attach and accumulate in artificial submerged structures, represents a serious economic and environmental issue worldwide. The discovery of new non-toxic and eco-friendly antifouling systems to control or prevent biofouling is, therefore, a practical and urgent need. In this work, the antifouling activity of a series of 24 xanthones, with chemical similarities to natural products, was exploited. Nine (1, 2, 4, 6, 8, 16, 19, 21, and 23) of the tested xanthones presented highly significant anti-settlement responses at 50 μM against the settlement of mussel Mytilus galloprovincialis larvae and low toxicity to this macrofouling species. Xanthones 21 and 23 emerged as the most effective larval settlement inhibitors (EC₅₀ = 7.28 and 3.57 µM, respectively). Additionally, xanthone 23 exhibited a therapeutic ratio (LC₅₀/EC₅₀) > 15, as required by the US Navy program attesting its suitability as natural antifouling agents. From the nine tested xanthones, none of the compounds were found to significantly inhibit the growth of the marine biofilm-forming bacterial strains tested. Xanthones 4, 6, 8, 16, 19, 21, and 23 were found to be non-toxic to the marine non-target species Artemia salina (<10% mortality at 50 μM). Insights on the antifouling mode of action of the hit xanthones 21 and 23 suggest that these two compounds affected similar molecular targets and cellular processes in mussel larvae, including that related to mussel adhesion capacity. This work exposes for the first time the relevance of C-1 aminated xanthones with a 3,4-dioxygenated pattern of substitution as new non-toxic products to prevent marine biofouling.  相似文献   

A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans     

Andrei V. Grinchenko  Alex von Kriegsheim  Nikita A. Shved  Anna E. Egorova  Diana V. Ilyaskina  Tatiana D. Karp  Nikolay V. Goncharov  Irina Y. Petrova  Vadim V. Kumeiko 《Marine drugs》2021,19(12)

C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin-like manner. A new protein MkC1qDC from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific was purified. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC sequence was assembled using de novo mass-spectrometry peptide sequencing complemented with N-terminal Edman’s degradation, and included 176 amino acid residues with molecular mass of 19 kDa displaying high homology to bivalve C1qDC proteins. MkC1qDC demonstrated antibacterial properties against Gram-negative and Gram-positive strains. MkC1qDC binds to a number of saccharides in Ca²⁺-dependent manner which characterized by structural meta-similarity in acidic group enrichment of galactose and mannose derivatives incorporated in diversified molecular species of glycans. Alginate, κ-carrageenan, fucoidan, and pectin were found to be highly effective inhibitors of MkC1qDC activity. Yeast mannan, lipopolysaccharide (LPS), peptidoglycan (PGN) and mucin showed an inhibitory effect at concentrations three orders of magnitude greater than for the most effective saccharides. MkC1qDC localized to the mussel hemal system and interstitial compartment. Intriguingly, MkC1qDC was found to suppress proliferation of human adenocarcinoma HeLa cells in a dose-dependent manner, indicating to the biomedical potential of MkC1qDC protein.  相似文献   

Fishing efficiency of competitive largemouth bass tournament anglers has increased since early 21st century     

Thomas M. Detmer  Kyle J. Broadway  Joseph J. Parkos  Matthew J. Diana  David H. Wahl 《Fisheries Management and Ecology》2020,27(5):540-543

Tournament fishing has risen in popularity over the last half a century. As such, social and financial incentives combined with technological advancements are expected to drive changes in angler's capacity to exploit tournament‐eligible fish stocks, as has been observed in commercial fisheries. The aim of this study was to quantify temporal trends in angler efficiency and their ability to exploit a given fish stock relative to effort in largemouth bass fishing tournaments. A collective analysis across seven Illinois reservoirs comparing change through time in angler catch rates and relative population abundances indicated that angler efficiency has generally improved through time. For the decade from 2005 to 2015, a greater than threefold increase in the efficiency of anglers to exploit a static population of largemouth bass was estimated. Anglers have become more efficient at exploiting populations, which is likely to influence management decisions in the future, particularly in harvest‐orientated fisheries and those reliant upon fishery‐dependent surveys.  相似文献   

Cover Image     

Diana Maria Cuesta‐Gomez  Juan Pablo Lazo  M. del Pilar Snchez‐Saavedra 《Aquaculture Research》2020,51(8):i-i

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