甘蔗根癌农杆菌介导转化海藻糖合酶基因获得抗渗透胁迫能力增强植株   总被引：27，自引：0，他引：27  

王自章  张树珍  杨本鹏  李杨瑞 《中国农业科学》2003,36(2):140-146

 由双拷贝CaMV35S启动子驱动担子菌灰树花 (Grifolafrondosa)的海藻糖合酶基因 (TSase)构建植物表达载体 pBBBT ,通过三亲交配法将 pBBBT导入根癌农杆菌EHA10 5菌株 ,经根癌农杆菌介导转化甘蔗 (Saccha rumhybrid)栽培品种 ,以增强甘蔗的抗旱能力。结果表明 ,甘蔗胚性愈伤组织对EHA10 5菌株敏感 ,甘蔗外植体开始大量形成胚性愈伤组织时是感染的适宜时期 ,用膦丝菌素 (PPT)筛选 ,抗性植株发生频率平均为 4 .5 %。经PCR及dot Southern检测证明 ,TSase已经整合到甘蔗基因组中。部分转化植株根叶畸形、株型异常、生长缓慢。移栽到含PEG80 0 0 17.4 % (w/v)的MS培养基后 ,观察到转基因植株抗渗透胁迫能力增强  相似文献   

三种植物性杀虫剂对烟草码绢金龟生物活性的初步研究   总被引：2，自引：0，他引：2  

刘晓波杨本立  陈国华陈斌　吴兴如 《云南农业大学学报(自然科学版)》2001,16(3):188-190

室内生物活性测定表明,印楝素对烟草码绢金龟成虫具有较强的忌避作用和拒食作用,苦楝油作用稍弱,烟株表现出药害症状,除虫菊反而具有一定的诱集和刺激取食效果,这3种植物性杀虫剂的毒杀作用均很弱.根据1%印楝素安全、活性高,但药效期较短的特点,提出了用印楝素防治码绢金龟的意见.  相似文献   

长白山阔叶红松林CO2廓线和CO2贮存的动态     

韩士杰  林丽莎  于贵瑞  张军辉  吴家兵  赵晓松  关德新 《林业研究》2003,14(4):275-279

从1999年8至10月,2000年的4至6月,2002年8月至2003年9月,在平均树高为26米的长白山阔叶红松林内,用红外气体分析仪(2250D,LI-CORInc.和LI-COR,820)测定了不同高度的二氧化碳浓度。根据测定的数据,分析了阔叶红松林二氧化碳廓线的日变化和季节变化动态。结果表明:CO2浓度的垂直分布在白天和夜间是不同的,在接近地面处CO2浓度始终最大。从季节CO2廓线看出,在植物生长季节林冠处CO2浓度有明显的成层现象,不同高度(60~2.5m)的CO2浓度3月份变化较小差值为10mmol穖ol-1,而在7月份变化较大,差值为60mmol穖ol-1。7月份林冠处(22,26,32m)CO2浓度梯度较大,浓度差为8mmol穖ol-1。计算位于涡度相关仪器之下的40米高空气柱中CO2贮存状况表明,年际贮存是负值,但对NEE的贡献很小。图4参11。  相似文献   

Application of digital photogrammetry to measure distribution of tree postions     

ZhangChao ZhangQing WangXuefeng 《中国林学(英文版)》2004,6(2):16-20

The application of digital photogrammetry to measure distribution of tree positions with stereo image couple is introduced in detail, and the procedure of stereo vision applied in forestry environment is explored. Nonlinear error in measure model is adopted in the camera calibration; the interactive correlation matching is used under constraint of epipolar line and edge of tree detected by Canny operator. Results prove that application of digital photogrammetry technology to measure distribution of tree positions can meet demand of precision in experimental conditions.  相似文献   

风对林木的影响   总被引：7，自引：0，他引：7  

朱教君  刘足根MATSUZAKITakeshi GONDAYutaka 《林业研究》2004,15(2):153-160

风除对森林造成了危害外,还对树木的生长、形态以及森林生态等产生影响。随着全球气候变化的发展,更多和更强的暴风出现的危险性日益增加。为了更好地理解风害对树木、林分和森林生态系统的影响,为森林经营管理提供依据,本文对近10年来国际上有关风对林木影响内容进行归纳总结。结果表明,风对林木影响的研究主要在以下几个方面取得了重要的进展：1)风与林木的空气动力学关系;2)树木在风力荷载下其适应性的生长机理;3)树木对风的生理响应：4)森林风害的危险评估。所有这些研究大都在人工用材林中进行的。此外,本文还介绍了该研究领域需要开展的其它研究,如1)天然林或天然次生林的风害研究;2)风害形成的林窗和森林动态研究;3)风害对森林生态主要过程的影响研究;4)．风害与森林管理研究。图3参61。  相似文献   

长白山典型阔叶红松林土壤二氧化碳排放通量     

王琛瑞  吴劼  梁战备  黄国宏 《林业研究》2004,15(4):268-272

随着大气CO2浓度的升高,主要由其引起的温室效应与对生物新陈代谢的影响变得越来越显著。森林生态系统在全球碳循环中扮演着重要的角色。为了评估和理解森林土壤CO2通量及其随空气和土壤温度的季节和昼夜变化规律,我们在长白山北坡典型阔叶红松林内利用静态箱技术进行了原位观测。实验在整个生长季(6月初至9月末)昼夜进行,利用气相色谱进行气体分析。结果表明: 长白山阔叶红松林土壤是大气二氧化碳源,其CO2通量具有明显的季节和昼夜变化规律。通量的变化范围是(0.30-2.42)μmol穖-2穝-1,平均值为0.98μmol穖-2穝-1。土壤CO2排放的季节规律表明,土壤CO2通量的变化与气温和土壤温度的变化有关。CO2平均通量的最大值出现在7月((1.27±23%)μmol穖-2穝-1),最小值出现在9月((0.5±28%)μmol穖-2穝-1)。土壤CO2的昼夜波动与土壤温度变化有关,而在时间上滞后于温度的变化。森林下垫面土壤CO2通量与土壤温度显著相关,与6cm深度土层温度相关系数最大。基于气温和土壤温度计算的Q10值范围为2.09-3.40。图2表3参37。  相似文献   

The contribution of root respiration of<Emphasis Type="Italic">Pinus koraiensis</Emphasis> seedlings to total soil respiration under elevated CO<Subscript>2</Subscript> concentrations   总被引：5，自引：0，他引：5  

Ying?Liu  Shi-jie?HanEmail author  Xue-feng?Li  Yu-mei?Zhou  Jun-hui?Zhang  Xia?Jia 《林业研究》2004,15(3):187-191

The impacts of elevated atmospheric CO₂ concentrations (500 μmol·mol⁻¹ and 700 μmol·mol⁻¹) on total soil respiration and the contribution of root respiration ofPinus koraiensis seedlings were investigated from May to October in 2003 at the Research Station of Changbai Mountain Forest Ecosystems, Chinese Academy of Sciences, Jilin Province, China. After four growing seasons in top-open chambers exposed to elevated CO₂, the total soil respiration and roots respiration ofPinus koraiensis seedlings were measured by a Li-6400-09 soil CO₂ flux chamber. Three PVC cylinders in each chamber were inserted about 30 cm into the soil instantaneously to terminate the supply of current photosynthates from the tree canopy to roots for separating the root respiration from total soil respiration. Soil respirations both inside and outside of the cylinders were measured on June 16, August 20 and October 8, respectively. The results indicated that: there was a marked diurnal change in air temperature and soil temperature at depth of 5 cm on June 16, the maximum of soil temperature at depth of 5 cm lagged behind that of air temperature, no differences in temperature between treatments were found (P>0.05). The total soil respiration and soil respiration with roots severed showed strong diurnal and seasonal patterns. There was marked difference in total soil respiration and soil respiration with roots severed between treatments (P<0.01); Mean total soil respiration and contribution of root under different treatments were 3.26, 4.78 and 1.47 μmol·m⁻²·s⁻¹, 11.5%, 43.1% and 27.9% on June 16, August 20 and October 8, respectively. Foundation item: This study was supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (KZCX1-SW-01) and the National Natural Science Foundation of China (30070158). Biography: LIU Ying (1976-), female, Ph. D. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China. Responsible editor: Song Funan  相似文献   

Characterisation of and changes in the atmospheric deposition situation in German forest ecosystems using multivariate statistics     

Nicole?WellbrockEmail author  Winfried?Riek  Barbara?Wolff 《European Journal of Forest Research》2005,124(4):261-271

Based on the results of the atmospheric deposition classification of the year 1989, a methodical approach should be introduced, which—based on the modelled total deposition rates—enables us to characterise the input situation of forest monitoring plots and to delimit load areas in Germany. In 1989, the deposition situation in nearly 1,800 forest monitoring sites (BZE/extensive Soil Condition Inventory) in Germany could be explained by four factors (or three, excluding sea salt impact) with the help of a factor analysis. The factor values were grouped into six deposition types with typical compounds and regional patterns. The classified input rates of the soil inventory plots adequately represent the stress situation and deposition changes in Germany. The application of the statistical approach on the level of Brandenburg clarifies the special local input situation. Due to the special combination of deposed elements, the sources of emissions can be characterised as well. When the soil inventory is repeated, a project planned for 2006, this approach can be used in order to determine homogenous areas for stratified data evaluation.  相似文献   

Correction to: Individual based modelling of fish migration in a 2-D river system: model description and case study     

Snyder  Marcía N.  Schumaker  Nathan H.  Ebersole  Joseph L.  Dunham  Jason B.  Comeleo  Randy L.  Keefer  Matthew L.  Leinenbach  Peter  Brookes  Allen  Cope  Ben  Wu  Jennifer  Palmer  John  Keenan  Druscilla 《Landscape Ecology》2019,34(5):1197-1197

Landscape Ecology - In the original publication of the article, the third author name has been misspelt. The correct name is given in this Correction. The original version of this article was revised.  相似文献   

我国农业机械化现状及发展趋势     

阿布来提&#;达吾提 《湖南农机》2017,44(2)

农业发展问题是中国经济增长的根本问题,在经济发展过程中,农业对国民经济具有不可替代的贡献作用.农业机械是现代农业的基础装备,对提高农业劳动生产率、增加农产品供给、保证农业稳步发展起了到至关重要的作用.为此,文章论述了中国农业机械化的现状,探讨了今后中国现代农业机械化的发展趋势,以期为我国农业机械行业的发展提供一定的参考.  相似文献