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61.
BackgroundBrucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes.ObjectiveTo describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does.MethodsTwelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 µL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100 µL of an inoculum containing 109 CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination.ResultsBilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs.ConclusionVertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue.  相似文献   
62.
Leaf blight is a common disease affecting Sansevieria trifasciata in many countries, including Malaysia. In the present study, Fusarium isolates were consistently recovered from the diseased leaves collected from various locations throughout the country. Based on morphology and multigene phylogenetic analysis using mitochondrial small subunit (mtSSU), intergenic spacer region (IGS) and translation elongation factor 1-α (TEF1-α) gene sequences, seven Fusarium species were identified, with F. oxysporum being the most prevalent (67.6%) among 34 isolates. Pathogenicity tests resulted in the discovery of pathogenic isolates that belonged to F. oxysporum, F. proliferatum, and F. pseudocircinatum, whereas all isolates of F. brachygibbosum, F. concentricum, F. mangiferae, and F. solani were nonpathogenic. The results suggest that several Fusarium species are accountable for causing disease on S. trifasciata in Malaysia.  相似文献   
63.
Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh.  相似文献   
64.
The influence of dietary supplementation of Lactobacillus acidophilus, on growth performance, digestive enzyme activities, gut histomorphology and gut microflora were evaluated in juveniles striped catfish (Pangasianodon hypophthalmus). Five experimental diets were formulated by supplementing 0, 103, 105, 107 and 109 CFU/g L. acidophilus in fishmeal and casein‐based semi‐purified diet. Triplicate groups of striped catfish (21.69 ± 0.18 g) were stocked in 15 fiberglass tanks with stocking density of 25 individuals per tank and fed twice daily at 2.5% of the fish body weight for 12 weeks. Weight gain, specific growth rate, feed conversion ratio and the protein efficiency ratio were significantly higher in fish fed with 105 and 107CFU/g L. acidophilussupplemented diets compared with the other treatment groups. Compared with the control and fish fed low (103 CFU/g) L. acidophilus supplementation, those fed with 105 and 107 CFU/g had significantly higher (p < 0.05) apparent protein digestibility. Inclusion of L. acidophilus at 107 CFU/g diet significantly increased amylase, protease and lipase activities. Microscopic analysis showed that the villi length in both the anterior and posterior gut and microvilli length in the posterior gut increased significantly in fish fed L. acidophilus supplementation at 105 and 107 CFU/g of diet. The fish fed L. acidophilus supplemented diets significantly increased the total lactic acid bacteria counts in the gut of striped catfish compared with the control‐fed group. Based on gut histomorphology and growth performance, inclusion of L. acidophilus at 105 CFU/g appears to have the most positive effect on striped catfish farming.  相似文献   
65.
Lodging has limited yield and potential of increasing yield in rice. Lodging could be minimized by increasing the mechanical or physical strength of the rice culm. Silicon (Si) is associated with sturdiness and rigidity. Therefore, the objectives of this experiment were to evaluate the effects and optimum rate of Si application to improve yield, growth, and lodging resistance of MR219, a popular rice variety in Malaysia. Si, in the form of fertilizer, was applied at the onset of reproductive stage as topdressing. All Si-treated plants showed an increase in number of tillers, number of spikelets per panicle, number of effective tillers, percentage of filled spikelets, and weight per panicle in Si-treated plants as compared to control. Lodging resistance was higher in plants treated with Si as shown by bending parameters. Si-treated plants showed significant increase in flag leaf area, chlorophyll content, and Si content.  相似文献   
66.
A field experiment was conducted to study the effects of coated urea with urease inhibitor [copper (Cu) and zinc (Zn)], nitrification inhibitor (DMPP), biochar and geopolymer on ammonium, nitrate, Cu, Zn content and crop yield of maize. The treatments were composed of urea alone (control), urea coated Cu and Zn (UCuZn), urea coated with Cu, Zn, and DMPP (UCuZnDMPP), urea impregnated with biochar (Ubio) and urea coated with geopolymer (Ug2). Data showed that treatments with Cu, Zn, and DMPP produced lower ammonium (NH4) and nitrate (NO3) in UCuZn and UCuZnDMPP while they had the highest concentration of Cu and Zn in soil and plant tissues. Plots treated with UCuZn and UCuZnDMPP produced maximum N concentrations in grains and yield, with increases by 79.5% and 74.1%, respectively, as compared with urea (control). This finding demonstrates that by slow down the hydrolysis and nitrification process using urease and nitrification inhibitor were beneficial to increased N uptake, ultimately produced higher yield.  相似文献   
67.
Biochar application has been received much attention because biochar can improve the fertilizer utilization efficiency of soil. However, the effect of biochar produced at different temperature on the nutrient retention and leaching remains poorly understood. In this study, we observed the nutrients leaching from a sandy loam soil amended with biochar produced at different temperature. The properties of biochars produced from wheat straw at four contrasting pyrolysis temperatures (250, 350, 450, and 550°C) showed that increasing pyrolysis temperature increased pH value and specific surface area but reduced the electrical conductivity and cation exchange capacity. With the temperature increased, the nitrogen loss was significant decreased (p > 0.05) from 109.6 mg to 53.3 mg in biochar amended soil. However, dissolved organic carbon (DOC), available P, Na and K were significant increased (p > 0.05). These results demonstrate that the pyrolytic temperature has a great influence on biochar properties, which in turn affect the leaching of the available nutrients.  相似文献   
68.
Gonadotropin (GTH) hormones are glycoprotein which stimulates gonadal maturation in vertebrates. Follicle stimulating hormone is involved in initiation of gametogenesis and regulation of gonadal growth. FSHβ has been cloned and characterized from the brain of Catla catla. The FSHβ full‐length of cDNA sequence of 523 bp comprised 3, 394 and 128 bp of 5′‐UTR, open reading frame (ORF) 3′‐UTR respectively. The coding region of C. catla FSHβ encoded a peptide of 130 amino acids. Phylogenetic analysis of C. catla FSHβ deduced amino acid sequence showed high similarity with Gobiocypris rarus followed by goldfish, Carassius auratus. The qPCR result shows that FSHβ mRNA is mainly expressed in pituitary while moderate and low expression was observed in testis and ovary respectively. Chitosan‐nanoconjugated kisspeptin‐10 (CK‐10) of particle size 125 nm, polydispersity index of 0.335 to 0.65 and zeta potential of ?34.95 mV were synthesized and evaluated at against naked kisspeptin‐10 for their reproductive hormonal profile. Treatment of fish with CK‐10 showed controlled and sustained surge of the reproductive hormones (FSH & LH) with peak at 12 h. The hormone levels of naked kisspeptin‐10 treated fish decline after 6 h. The sustained release of this CK‐10 will help in reducing maturation age, synchronization of ovulation and spawning in fish. This is the first report on use of chitosan‐nanoconjugated kisspeptin‐10 (CK‐10) for reproduction in fish.  相似文献   
69.
Exposure to heat‐shock protein (Hsp) stimulating factors induces Hsp accumulation and confers tolerance to lethal ammonia stress on the common carp Cyprinus carpio. This study investigated whether a non‐lethal heat shock bestowed similar protective effects against ammonia and induced thermotolerance, both thought to be rendered by increased amounts of Hsps. The 30‐min lethal temperature (30 min LHT) and 1‐h lethal ammonia concentration (1 h LCT) for this species occurred at 41°C and 14.2 mg/L NH3 respectively. Heating juvenile carp (5 cm) from 28°C to 32, 34 and 38°C, with a subsequent 8‐h recovery period augmented tolerance to lethal heat and ammonia perturbation by two to threefold as compared with animals held at 28°C. Protection occurred in conjunction with Hsp70 accumulation in gills, substantiating the role of this Hsp in enhancing the stress tolerance of common carp.  相似文献   
70.
The production performance of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) and freshwater prawn (Macrobrachium rosenbergii) in periphyton‐based systems were studied in farmers' ponds at Mymensingh, Bangladesh. Fifteen ponds (200–300 m2 area and 1.0–1.5 m in depth) were used to compare five stocking ratios in triplicate: 100% GIFT, 75% GIFT plus 25% prawn, 50% GIFT plus 50% prawn, 25% GIFT plus 75% prawn and 100% prawn. Ponds were stocked at a total density of 20 000 GIFT and/or prawn ha?1. Bamboo poles (mean diameter 6.2 cm and 5.5 pole m?2) were posted in pond bottoms vertically as periphyton substrate. Periphyton biomass in terms of dry matter (DM), ash‐free DM and chlorophyll a were significantly higher in ponds stocked with prawn alone than in ponds with different combinations of GIFT and prawn. Survival of GIFT was significantly lower in ponds stocked with 100% GIFT (monoculture) whereas, that of prawn was significantly higher in its monoculture ponds indicating detrimental effects of GIFT on prawn's survival. Individual weight gains for both species were significantly higher in polyculture than in monoculture. The highest total fish and prawn yield (1623 kg GIFT and 30 kg prawn ha?1) over 125–140 days culture period was recorded in ponds with 75% GIFT and 25% prawn followed by 100% GIFT alone (1549 kg ha?1), 50% GIFT plus 50% prawn (1114 kg GIFT and 68 kg prawn ha?1), 25% GIFT plus 75% prawn (574 kg GIFT and 129 kg prawn ha?1) and 100% prawn alone (157 kg ha?1). This combination also gave the highest economic return. Therefore, a stocking ratio of 75% GIFT plus 25% prawn at a total density of 20 000 ha?1 appeared to be the best stocking ratio in terms of fish production as well as economics for a periphyton‐based polyculture system.  相似文献   
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