棉花SSR-PCR过程中聚丙烯酰胺凝胶电泳操作常见问题的解决与优化     

张俊  潘兆娥  张亚楠  周琳 《中国棉花》2016,43(2):10-13

在大批量聚丙烯酰胺凝胶电泳过程中,难免会出现条带空白、弯曲或者拖尾等各种异常现象。本文以棉花DNA为例,综述了多年实验操作中遇到的突发情况及解决方法,为广大科研工作者提供参考。  相似文献   

猪胆囊中沙门菌L型的分离培养与invA基因检测     

佘晓玲  王涛  丁文静  吴文娟  潘耀振  汤可立  王丹霓  王和 《动物医学进展》2015,(3):25-28

为了解猪胆囊中沙门菌L型携带情况,在贵阳市屠宰场采集970例健康生猪的胆囊组织与胆汁标本,用常规细菌学方法和非高渗分离培养法分离沙门菌及其细菌L型,用PCR和核酸序列分析方法对稳定L型纯培养物进行沙门菌的invA基因检测。结果显示,970例生猪胆囊标本未检出沙门菌细菌型,细菌L型检出率为8.25%;80例细菌L型分离物中有50例invA检测阳性,占5.15%;占细菌L型阳性分离物62.50%。研究结果为生猪胆囊沙门菌L型感染的流行病学及其检查提供了依据。  相似文献   

蓝莓提取物对改善小鼠睡眠剥夺型记忆力减退的影响研究     

杨闯  潘顺圆  刘鑫悦  李震东  杨帆  裴娜  孙磊  任永宁  孔凡志 《黑龙江八一农垦大学学报》2020,32(6):38-42

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玉米植株数量性状配合力及其利用价值分析     

胡军  魏开锋  刘万  郭鹏程  徐风雨  潘洪玉  张鑫生 《黑龙江农业科学》2020,(1):1-8

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美洲黑杨杂交子代苗期性状遗传变异及选择          下载免费PDF全文

严艳兵  潘惠新 《浙江农林大学学报》2021,38(6):1144-1152

  目的  分析美洲黑杨Populus deltoides杂种苗期生长性状和叶片性状的遗传变异及遗传相关性，为杨树新品种选育提供材料。  方法  以美洲黑杨不同品种为亲本进行杂交试验，测定了9个杂交组合子代苗期生长性状和叶片性状，并通过方差分析、遗传参数估算、遗传相关分析及通径分析等，了解美洲黑杨杂种生长性状和叶片性状的遗传变异规律及性状间遗传互作，并依此开展美洲黑杨优良杂交组合的联合选择。  结果  杂交组合间3个生长性状与5个叶片性状存在显著(P＜0.05)或极显著(P＜0.01)差异。苗高、地径、材积与叶长、叶宽、叶柄长、叶周长、叶面积的家系遗传力均达0.8以上，受强度遗传控制，遗传变异系数为8.6%(叶长)~31.13%(材积)，有利于优良杂交组合的选择。相关分析表明:叶长、叶宽、叶柄长、叶周长、叶面积与苗高、地径、材积间均达极显著正遗传相关(P＜0.01)。相关遗传进度分析表明:除叶形指数、侧脉夹角和叶宽基距外，其他叶片性状对3个生长性状的遗传相关进度和间接选择效率较高。通径分析表明:苗高和地径对材积的直接遗传控制作用较大，叶长、叶宽、叶柄长、叶面积和叶周长通过苗高和地径对材积产生较大的间接遗传控制作用。利用综合指数选择法开展美洲黑杨9个杂交组合的生长与叶片性状联合选择，选出3个速生优质的杂交组合(B106×NL15、S3239×NL15、NL447×SY2)，材积遗传增益达26.90%。  结论  美洲黑杨杂交组合子代1年生苗的3个生长性状和5个叶片性状均存在丰富变异，遗传互作显著；苗高和地径对材积的直接作用最大，5个叶片性状对材积也产生较大的间接控制效应。综合指数选择法能有效地选出速生优质杂交组合，材积遗传增益较高，杨树遗传改良效果较好。表8参18  相似文献   

雪山草鸡感染传染性法氏囊病病毒新型变异株的鉴定     

姜楠  王雨龙  张文英  牛鑫鑫  刘长军  高玉龙  高立  崔红玉  李凯  潘青  张艳萍  刘爱晶  王笑梅  祁小乐 《中国动物检疫》2021,38(5):119-125

传染性法氏囊病病毒(infectious bursal disease virus,IBDV)新型变异株正在我国广泛蔓延,给商品肉鸡群和蛋鸡群带来了新的威胁。本研究对一个疑似发生非典型传染性法氏囊病的地方品种雪山草鸡群进行了RT-PCR检测,并对分离的3株毒株进行了基因测序及序列分析。结果显示,引起该鸡群发病的病原是IBDV新型变异株,属于A2dB1基因型;这3个毒株的VP2高变区编码蛋白上含有IBDV变异株的特征性氨基酸,也具有IBDV新型变异株的独特氨基酸。结果提示,我国地方品种鸡群中也有IBDV新型变异株的流行,现有的部分商品化IBDV疫苗已不能有效预防IBDV新型变异株的流行。  相似文献   

彩色多普勒超声对直径3 cm以上肾上腺肿瘤的诊断价值     

徐园园  孙医学  陆芳  石彦  潘如风  仝贺 《河北北方学院学报(自然科学版)》2020,36(6):11-12,15

目的探讨彩色多普勒超声对直径大于3 cm肾上腺肿瘤的诊断价值。方法使用彩色多普勒超声检测43例直径大于3 cm肾上腺肿瘤的患者,分析超声图像特征及相关临床资料。结果 43例患者中超声诊断正确41例,误诊1例,漏诊1例;定位诊断率约95.3%。肿瘤以单侧单发为主,其中恶性转移瘤多来自肺癌,且大部分与原发灶来自同侧。肾上腺肿瘤形态多规则,内部回声多为均匀低回声,少许瘤体呈高回声或混合性回声,大部分肿瘤无明显血流信号。结论超声检查对直径大于3 cm肾上腺肿瘤的诊断及临床分型有重要价值。  相似文献   

Quantitative trait loci for fruit size and flowering time-related traits under domestication and diversifying selection in cucumber (Cucumis sativus)     

Yunyan Sheng  Yupeng Pan  Yuhong Li  Luming Yang  Yiqun Weng 《Plant Breeding》2020,139(1):176-191

In cucumber, the genetic basis of traits under domestication and/or diversifying selection is not well understood. Here, we reported QTL mapping for flowering time and fruit size-related traits with segregating populations derived from a cultivated × wild cross. Phenotypic data of flowering time (FT), fruit size (FS), fruit number (FN) and fruit weight per plant (FW) were collected in multiple environments. QTL analysis identified 19 QTL for these traits. We found that the major-effect QTL FT1.1 played an important role in regulating flowering time in cultivated cucumber, whereas the minor-effect QTL FT6.3 contributed to photoperiod sensitive flowering time during domestication. Two novel consensus FS QTL, FS1.4 and FS2.3, seem to be the targets of selection during breeding for the US processing cucumber. All other FS QTL were co-localized with previously detected QTL using populations derived from cultivated cucumbers, suggesting that they were under selection during both initial domestication and subsequent improvement. Results from this study also suggested that the wild cucumber is a useful resource for capturing positive transgressive segregation and novel alleles that could be explored in cucumber breeding.  相似文献   

β-carotene attenuates weaning-induced apoptosis via inhibition of PERK-CHOP and IRE1-JNK/p38 MAPK signalling pathways in piglet jejunum     

Ruonan Li  Yu Yang  Pan Hong  Ziqi Zhang  Lingqian Li  Junnan Hui  Xin Zheng 《Journal of animal physiology and animal nutrition》2020,104(1):280-290

Weaning may cause oxidative injury, immune response impairment, apoptosis and other injuries in piglets. Oxidative and endoplasmic reticulum stress (ERS) can elicit inflammatory responses, and persistent oxidative and ERS also may lead to apoptotic cascades, which is associated with the pathogenesis of multiple diseases. β-carotene, a natural carotenoid, has potential anti-inflammatory and antioxidant functions. However, the effect of β-carotene on apoptosis in weaned piglets and the detailed molecular mechanism remain unclear. In this study, we found that β-carotene decreased malondialdehyde (MDA) levels and increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in piglet serum. β-carotene could inhibit the mRNA levels of caspase-3 significantly, but had no significant inhibitory effect of the mRNA levels of caspase-9 and caspase-12 in the piglet jejunum. In addition, β-carotene decreased the activation of GRP78, CHOP, and JNK/p38 MAPK and the ratio of Bax/Bcl-2. Furthermore, β-carotene had a significant influence on the activation of ERS and apoptosis-related signals in TG-induced IPEC-J2. In the present study, β-carotene pre-treatment attenuated the ratio of Bax/Bcl-2 and prevented TG-induced increases in the level of PERK-CHOP and IRE1-JNK/p38 MAPK pathway activation in a dose-dependent manner. Overall, these findings indicate that β-carotene may protect weaning-induced apoptosis through inhibiting ERS.  相似文献   

灰霉菌侵染对番茄幼苗活性氧积累及抗氧化酶活性的影响     

何娟  潘光军  官涵杰  陆光磊  陈江艳  须文 《蔬菜》2020,(5):15-20

为进一步分析活性氧和抗氧化防御系统在番茄抗病信号调控机制中的作用,为提高产量和品质提供科学依据,以4叶1心的番茄幼苗为试验材料,比较番茄幼苗受灰霉菌侵染后叶片O_2~-·以及抗氧化酶、内源褪黑素等的变化。结果表明:番茄幼苗接种灰霉菌后,随着时间的延长,幼苗叶片上病斑斑点逐渐增多,灰霉菌含量显著增多,叶色逐渐变浅,植株表现明显的发病症状;灰霉病菌侵染后番茄体内O_2~-·活性爆发,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降,过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性上升,褪黑素含量增加;上述变化都是番茄幼苗为了抵御灰霉菌的生物胁迫而发生的应激反应。综上,植物在遭受病原菌等生物胁迫时不能简单地推断SOD、CAT和POD等抗氧化酶活性一定升高,植物抵御病原菌侵染更多地依赖于抗性信号路径中信号物质的激活和积累。  相似文献