The minimum dietary requirement of vitamin C in Atlantic salmon (Salmo salar) fry using Ca ascorbate-2-monophosphate as dietary source     

Kjartan Sandnes  Ole Torrissen  Rune Waagbø 《Fish physiology and biochemistry》1992,10(4):315-319

The minimum dietary vitamin C requirement for optimal growth and normal development in Atlantic salmon (Salmo salar) fry at the onset of feeding was studied, using Ca ascorbate-2-monophosphate (AP) as dietary source. The requirement was established by means of a feeding study lasting for 23 weeks from the beginning of feeding. The practical diets used were supplemented with AP at levels of 0, 10, 20, 40, 80 and 160 mg ascorbic acid (AA) equivalents/kg. Growth, mortality, hydroxyproline content in skin and backbone, and AA in liver were recorded to evaluate the results. The results suggest that the minimum dietary requirement for optimal growth and normal development is in the range of 10–20 mg AA equivalents/kg dry diet during the period studied.  相似文献   

Distribution and induction of cytochrome P450 1A1 in the rainbow trout brain     

Tommy Andersson  Anders Goksøyr 《Fish physiology and biochemistry》1994,13(4):335-342

Cytochrome P450 (CYP) 1A1 participates in the activation as well as detoxification of environmental pollutants such as aromatic hydrocarbons. This CYP form is also efficiently induced by aromatic hydrocarbons. The presence of CYP 1A1 in the brain might thus be of physiological and toxicological importance. In the present investigation on rainbow trout, the distribution of 7-ethoxyresorufin-O-deethylase (EROD) activity, a cytochrome CYP 1A1 catalyzed reaction, was measured in whole tissue homogenates from brain parts. In control fish, a relatively high activity was found in the rainbow trout olfactory bulb compared to the other brain parts. Although an EROD induction (3 to 7-fold) by β-naphthoflavone (BNF) was recorded in all brain parts from the rainbow trout, the highest induced activity was measured in the olfactory bulbs. To ascertain the distribution of EROD activity in cells, whole brain tissue was subfractionated by differential centrifugation. The fractionation scheme separated mitochondria (P2 fraction) and microsomes (P3 fraction) as determined by marker enzymes and electron microscopy. In control rainbow trout, a low EROD activity could be measured in the P2 fraction. BNF induced the EROD activity in both P2 and P3 fractions. Western blotting showed the induction by BNF of a protein band in the P2 and P3 fractions with a molecular mass around 58,000 when highly specific anti-cod CYP 1A1 antibodies were used. ELISA measurements confirmed the induction of CYP 1A1 protein in the rainbow trout brain subcellular fractions.  相似文献   

Compensatory growth in Atlantic halibut: Effect of starvation and subsequent feeding on growth, maturation, feed utilization and flesh quality     

Atle Foss  Albert K. Imsland  Erik Vikingstad  Sigurd O. Stefansson  Birgitta Norberg  Skjalg Pedersen  Trond Sandvik  Bjrn Roth 《Aquaculture (Amsterdam, Netherlands)》2009,290(3-4):304-310

A group of juvenile Atlantic halibut (mean, SD, 199.5 g, 44.7) was split into two experimental groups: one group was fed in excess twice a day and later five days a week (i.e. Control group), and the other group was starved for 5 weeks and then subsequently re-fed for 10 weeks (i.e. 5/10 starvation/re-fed group). This treatment was repeated for three years until the fish were harvested (mean final weight, 4.4 kg). A size +specific compensatory response was seen; partial compensation was observed in halibut weighing less than 500 g whereas full recovery growth was seen in halibut over 2 kg. Food consumption and feed conversion efficiency was measured during two full 5/10 starvation/re-fed cycles. The Control group consumed 86% more food in the measurement period, whereas the FCE 40% higher in the 5/10 starvation/re-fed group (FCE = 0.95) compared to the Control group (FCE = 0.68). In males lower gonadosomatic index (2.2 vs. 3.0) and plasma 11-ketotestosterone (0.22 vs. 1.26 ng ml^− 1) levels in the 5/10 starvation/re-fed group were observed during late winter and spring of 2006 (age 3 years) indicating higher age at 1st maturity. Starvation had a significant effect for improving the quality as starved fish had higher muscle pH (6.4 vs. 6.2), less gaping (0.1 vs. 0.3) and harder texture (61.4 vs. 57.6 N). Overall, the rearing on a repeated 5/10 starvation/re-fed regime for 3 years led to full growth compensation, higher feed conversion efficiency, lower male maturation and improved flesh quality.  相似文献   

Effects of Packaging Method on Salt-Cured Cod Yield and Quality During Storage     

Grete Hansen Aas  Olaug Taran Skjerdal  Iren Stoknes  Ingebrigt Bjørkevoll 《Journal Of Aquatic Food Product Technology》2013,22(3-4):149-161

Different packaging conditions of salt-cured cod fillets were studied during 2-years chilled storage. Yield and quality of salt-cured Atlantic cod fillets packed in sealed polystyrene boxes or in traditional cardboard boxes were compared. After 4 months, yield decreased by approximately 10% and decreased further when packed in cardboard boxes. Lipid oxidation was low after 12 months, and no microbiological spoilage was registered. The total amount of bacteria in the fish reduced with storage time, independent of packaging. The reddish color of halophilic bacteria was detected after 7–8 weeks at 18°C, but not if packed with MAP.  相似文献   

Degradation and effect of hydrogen peroxide in small‐scale recirculation aquaculture system biofilters     

Martin Sune Møller  Erik Arvin  Lars‐Flemming Pedersen 《Aquaculture Research》2010,41(8):1113-1122

From an environmental point of view, hydrogen peroxide (HP) has beneficial attributes compared with other disinfectants in terms of its ready degradation and neutral by‐products. The rapid degradation of HP can, however, cause difficulties with regard to safe and efficient water treatment when applied in different systems. In this study, we investigated the degradation kinetics of HP in biofilters from water recirculating aquaculture systems (RAS). The potential effect of HP on the nitrification process in the biofilters was also examined. Biofilter elements from two different pilot‐scale RAS were exposed to various HP treatments in batch experiments, and the HP concentration was found to follow an exponential decay. The biofilter ammonia and nitrite oxidation processes showed quick recuperation after exposure to a single dose of HP up to 30 mg L^?1. An average HP concentration of 10–13 mg L^?1 maintained over 3 h had a moderate inhibitory effect on the biofilter elements from one of the RAS with relatively high organic loading, while the nitrification was severely inhibited in the pilot‐scale biofilters from the other RAS with a relatively low organic loading. A pilot‐scale RAS, equipped with two biofilter units, both a moving‐bed (Biomedia) and a fixed‐bed (BIO‐BLOK^®) biofilter, was subjected to an average HP concentration of ～12 mg L^?1 for 3 h. The ammonium‐ and nitrite‐degrading efficiencies of both the Biomedia and the BIO‐BLOK^® filters were drastically reduced. The filters had not reverted to pre‐HP exposure efficiency after 24 h, suggesting a possible long‐term impact on the biofilters.  相似文献   

Digestibility of the defatted microalgae Nannochloropsis sp. and Desmodesmus sp. when fed to Atlantic salmon,Salmo salar          下载免费PDF全文

Y. Gong  H.A.D.S. Guterres  M. Huntley  M. Sørensen  V. Kiron 《Aquaculture Nutrition》2018,24(1):56-64

The aim of the study was to investigate the apparent digestibility coefficient (ADC) of defatted biomass derived from microalgae Nannochloropsis sp. and Desmodesmus sp. when fed to Atlantic salmon postsmolts in seawater. Two experiments, one employing cold‐pelleted and the other employing extruded pellets, were carried out to determine the ADC of dry matter (DM), protein, ash and energy. The test feeds consisted of a fishmeal‐based reference feed and the algal biomass, at a ratio of 70:30, and yttrium oxide as the inert marker. The ADC of DM and protein in fish fed microalgae‐incorporated cold‐pelleted feeds were significantly higher for Nannochloropsis sp. compared with Desmodesmus sp. The Nannochloropsis sp. inclusion in extruded feeds also led to higher ADCs of DM and energy compared with Desmodesmus sp. The extrusion processing significantly increased the ADC of ash in both defatted microalgae biomass, as well as ADCs of DM in Nannochloropsis and protein in Desmodesmus sp. In conclusion, the microalga Nannochloropsis sp. was more digestible than Desmodesmus sp., and extrusion processing can be used as a means to improve digestibility of certain nutrients.  相似文献   

Identification of Francisella sp. from Atlantic salmon, Salmo salar L., in Chile     

Birkbeck TH  Bordevik M  Frøystad MK  Baklien A 《Journal of fish diseases》2007,30(8):505-507

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Studies on uptake and intracellular processing of infectious pancreatic necrosis virus by Atlantic cod scavenger endothelial cells     

Martin-Armas M  Sommer AI  Smedsrød B 《Journal of fish diseases》2007,30(11):701-710

Previous work in our group has identified the scavenger endothelial cells (SECs) of heart endocardium in cod, Gadus morhua L., as the major site for elimination of both physiological and foreign macromolecular waste from the circulation. The present study was undertaken to establish the role of cod SECs in the clearance of virus. We focused on infectious pancreatic necrosis virus (IPNV) as it is a well-known virus with a broad host range, and causes significant economic losses in the salmon industry. Our results showed that cod SEC cultures infected by the IPNV produce high titres of new virus. Ligand-receptor inhibition experiments suggested that the virus did not enter the cells through any of the major endocytosis receptors of cod SECs. Yet, the infection lowered the capacity of the cells to endocytose ligands via the scavenger receptor. Inhibitors of receptor recycling and vesicle acidification did not affect virus infectivity. The finding that SEC cultures prepared from 25% of the cod produced high titres of IPNV without being infected in the laboratory, suggests that SECs of cod may serve as reservoirs for IPNV in persistently infected cod.  相似文献   

Pesticide contamination of the upper Elbe River and an adjacent floodplain area     

Karlsson  Anneli S.  Lesch  Michael  Weihermüller  Lutz  Thiele  Björn  Disko  Ulrich  Hofmann  Diana  Vereecken  Harry  Spielvogel  Sandra 《Journal of Soils and Sediments》2020,20(4):2067-2081

Journal of Soils and Sediments - Pesticide contamination of river waters is a global problem, and therefore, authorities regularly monitor the water quality status. Especially, flood events might...  相似文献   

Rate of decomposition of organic matter in soil as influenced by repeated air drying-rewetting and repeated additions of organic material     

L.H. Sørensen 《Soil biology & biochemistry》1974,6(5):287-292

Repeated air drying and rewetting of three soils followed by incubation at 20°C resulted in an increase in the rate of decomposition of a fraction of ¹⁴C labeled organic matter in the soils. The labeled organic matter originated from labeled glucose, cellulose and straw, respectively, metabolized in the soils during previous incubation periods ranging from 1.5 to 8 years.Air drying and rewetting every 30th day over an incubation period of 260–500 days caused an increase in the evolution of labeled CO₂ ranging from 16 to 121 per cent as compared to controls kept moist continuously. The effect of the treatment was least in the soil which had been incubated with the labeled material for the longest time.Additions of unlabeled, decomposable organic material also increased the rate of decomposition of the labeled organic matter. The evolution of labeled CO₂ during the 1st month of incubation after addition was in some cases 4–10 times larger than the evolution from the controls. During the continued incubation the evolution decreased almost to the level of the controls, indicating that the effect was related to the increased biological activity in the soils during decomposition of the added material.Three additions of organic material during the period of incubation resulted totally in an increase over the controls ranging from 36 to 146 per cent.  相似文献